-
No products found
because this supplier's products are not listed.
Hanyu Pan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Yang.17 The PD-1 blocking scFv E27 gene was synthesized by GENEWIZ (GENEWIZ, Suzhou, China) according to the previous report.36 3BNC117 CAR and E27 were cloned into a lentiviral vector (pTRPE-GFP-T2A-mRFP ...
-
No products found
because this supplier's products are not listed.
Colton D. Payne, et al.,
bioRxiv - Biochemistry 2020
Quote:
... A CS336X automatic peptide synthesizer (CSBio) was used to couple the remaining residues in the peptide chain ...
-
No products found
because this supplier's products are not listed.
Nikolas Nikolaou, et al.,
bioRxiv - Neuroscience 2020
Quote:
... This was removed and coverslips were incubated with fresh blocking solution containing anti-GFP 1:1000 (Torrey Pines Biolabs-TP401) and anti-SNRNP70 1:200 (Sigma-AV40276 ...
-
No products found
because this supplier's products are not listed.
Rachel J. Harding, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HTT protein was eluted with 1 cell paste volume of buffer supplemented with 250 μg/mL 3xFLAG peptide (Chempep) run twice over the anti-FLAG resin ...
-
No products found
because this supplier's products are not listed.
Gregory A. Breuer, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Blocking was performed in TBS-T with 5% BSA (Gold Biotechnology) for 1h at room temperature ...
-
No products found
because this supplier's products are not listed.
Francesca Boscolo Sesillo, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Slides stained for collagen I were first washed and then incubated in in blocking buffer (10% goat serum, 0.3% Triton x-100, 1% BSA (Gemini Bio-Products, 700-100P) in PBS ...
-
No products found
because this supplier's products are not listed.
Sebastian Boland, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Immunostaining for 2 hours was performed with the following primary antibodies (1:100 dilution in blocking buffer): GM2 (Tokyo Chemical Industry Cat#2576), LAMP1 (Cell Signaling Technology Cat#9091) ...
-
No products found
because this supplier's products are not listed.
Gemma L. Pearson, et al.,
bioRxiv - Cell Biology 2022
Quote:
... blocked for 1 h at room temperature with 1 X Blocking One solution (Nacalai USA Inc; San Diego, CA, USA). Sections were then incubated in the following primary antisera overnight at 4°C in 1 X PBS + 1% tween + 20% Blocking One solution ...
-
No products found
because this supplier's products are not listed.
Sherif Salah, et al.,
bioRxiv - Immunology 2022
Quote:
... and membrane protein peptide (by AMSBIO) were procured ...
-
No products found
because this supplier's products are not listed.
Simona Giunta, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Incubations with primary antibodies were conducted in blocking buffer for 1 h at room temperature using the following antibodies: ACA (1:500; 15-235-0001, Antibodies Incorporated), ATR (1:1000 ...
-
No products found
because this supplier's products are not listed.
Ji-Hoon Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... incubated with primary antibody against spike protein diluted 1:5,000 in blocking buffer (Elabscience, E-AB-V1006) overnight in a 37°C incubator with 5% CO2 injection ...
-
No products found
because this supplier's products are not listed.
Francisco Santos, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and blocking with 5% goat serum (Abbkine Scientific) and 0.3% Triton X-100 in PBS1x for 1h at RT with agitation ...
-
No products found
because this supplier's products are not listed.
D Muñoz-Reyes, et al.,
bioRxiv - Biochemistry 2022
Quote:
... NCS-1ΔH10/Ric-8A-P2 peptide and NCS-1ΔH10/Ric-8A-P3 peptide were performed using a Tycho NT.6 instrument (NanoTemper Technologies). Proteins at 10 μM in their corresponding final buffers (see above ...
-
No products found
because this supplier's products are not listed.
Antonio Dominguez-Meijide, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... a blocking step with 1.5% BSA (NZYTech, Lisbon, Portugal) was performed for 1 hour ...
-
No products found
because this supplier's products are not listed.
Sara R Roig, et al.,
bioRxiv - Cell Biology 2021
Quote:
The following peptides were purchased from EMC microcollections GmbH ...
-
No products found
because this supplier's products are not listed.
Mireia Rovira, et al.,
bioRxiv - Immunology 2022
Quote:
... Peptides were purified using SDB-RPS columns (Affinisep). Briefly ...
-
No products found
because this supplier's products are not listed.
Rick G. Kim, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Biotinylated peptides were searched using exogenous biotin (ExBio) on lysine as a variable modification ...
-
No products found
because this supplier's products are not listed.
Eric E. Gardner, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 0.05% Triton X-100 in 1X PBS pH 7.2 supplemented with mouse-on-mouse blocking reagent (Vector Biolabs; 1:50) and Fc receptor blocker reagent (Innovex Biosciences ...
-
No products found
because this supplier's products are not listed.
Jhansi L. Leslie, et al.,
bioRxiv - Microbiology 2019
Quote:
... each plate had a positive control consisting of toxin coated wells reacted with mouse TcdA monoclonal antibody TGC2 diluted 1:5,000 in blocking buffer (antibodies-online.com ABIN335169). The optical density at 410nm and 650nm was recorded on a VersaMax plate reader (Molecular Devices ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... antibodies blocking the type I IFN receptor (mouse anti-human IFNAR2 antibody, clone MMHAR-2, PBL Assay Science Cat#21385-1) or isotype control (Ultra-LEAF Purified mouse IgG2a ...
-
No products found
because this supplier's products are not listed.
Nikola Cousin, et al.,
bioRxiv - Immunology 2021
Quote:
OT-1 effector T cells were generated by ex vivo culture of total Ly5.1+ OT-1 splenocytes in T cell medium supplemented with 1 ng/ml SIINFEKL peptide and 100 U/ml recombinant mouse IL-2 (ImmunoTools) for 72 h ...
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No products found
Pooja Khanna, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Membranes were blocked with Advanblock blocking buffer (Advansta R-03726-E10) for 1 hour at room temperature and incubated at 4°C in primary antibody overnight ...
-
No products found
because this supplier's products are not listed.
Emily Tubbs, et al.,
bioRxiv - Bioengineering 2024
Quote:
... The cells were permeabilized by a blocking buffer containing FBS (ScienCell Research Laboratories ...
-
No products found
because this supplier's products are not listed.
Natalie S. Haddad, et al.,
bioRxiv - Immunology 2020
Quote:
... Coupled microspheres were washed four times in a blocking buffer consisting of 1% BSA (Bovine Serum Albumin; Boston Bioproducts; Ashland, MA, USA), 1X PBS (VWR ...
-
No products found
because this supplier's products are not listed.
Wei Ding, Walter A. Koltun, Gregory S. Yochum,
bioRxiv - Cell Biology 2024
Quote:
... The cover slips were then incubated with the following primary antibodies diluted in blocking/staining buffer overnight at 4°C: anti-MUC5B (1:200, Atlas Antibodies, HPA008246); anti-E-cadherin (1:200 ...
-
No products found
because this supplier's products are not listed.
Douek-Maba Orit, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... followed by an overnight incubation at 4°C in blocking solution with anti phospho-histone 3 (PhH3) antibody or anti-caspase 3 (Cas3) antibody (both 1:300; Lifespan Bioscience. Washington US). Next ...
-
No products found
because this supplier's products are not listed.
Pragya Parashara, et al.,
bioRxiv - Cell Biology 2024
Quote:
... PLK1PBD was concentrated to 6 mg/ml and mixed with two times molar excess of S54ph peptide The mixture was incubated on ice for at least 1 h before setting up crystallisation trays with Morpheus® (Molecular Dimensions) screen using the ART Robbins Crystal Gryphon crystallisation robot in 96-well sitting drop MRC plates at 18ºC ...
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The CY3 (Red) Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically...
Cat# 5276-60UG,
0.3 mg, USD $290.0
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Amber L. Altrieth, et al.,
bioRxiv - Cell Biology 2023
Quote:
Collagen hybridizing peptide (CHP) conjugated to 5-carboxyfluorescein (5-FAM) (Advanced BioMatrix) was solubilized per manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Ishraq Alshanqiti, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Michael Caterina at Johns Hopkins University) and guinea pig anti-calcitonin gene-related peptides (CGRP) (1:1,000; Peninsula Laboratories International, San Carlos, CA, USA)) ...
-
No products found
because this supplier's products are not listed.
Bijal A. Parikh, et al.,
bioRxiv - Immunology 2019
Quote:
... Fc receptor blocking was performed with 2.4G2 (anti-FcγRII/III) hybridoma (American Type Culture Collection) culture supernatants ...
-
No products found
because this supplier's products are not listed.
Marcin Pęziński, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and 100 nM Actistain conjugated to AlexaFluor 555 (in blocking buffer; catalog no. PHDH1, Cytoskeleton), followed by three washes with PBS ...
-
No products found
because this supplier's products are not listed.
Emilio Boada-Romero, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Experiments using anti-PS blocking antibody were performed in µ-Slide Angiogeneis chambers (IBIDI, 81506) and cell numbers were scaled down accordingly.
-
No products found
because this supplier's products are not listed.
Logan S. Richards, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The peptide crystals were harvested from hanging drops using CryoLoops™ from Hampton Research with no additional cryoprotectant other than the MPD already present and flash-frozen in liquid nitrogen ...
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The Poly DYKDDDDK (FLAG) Peptide lyophilized powder was synthesized by 23 amino acid residues...
Cat# B23111, SKU# B23111-4mg,
4mg, $299.00
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Caillan Crowe-McAuliffe, et al.,
bioRxiv - Biochemistry 2020
Quote:
... RqcH-GS-FLAG3 was eluted by addition of 200 μL opening buffer containing 0.1 mg/mL poly-FLAG peptide (Biotool, Bimake) for 45 min on a turning wheel ...
-
No products found
because this supplier's products are not listed.
Natalie Schindler, et al.,
bioRxiv - Genetics 2022
Quote:
... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
-
No products found
because this supplier's products are not listed.
Matthew P. Ostrowski, et al.,
bioRxiv - Microbiology 2021
Quote:
... sequences were designed to remove N-terminal signaling peptides and to add a histidine tag for immobilized metal affinity chromatography (IMAC) (in many cases using the Lucigen MA101-Expresso-T7-Cloning-&-Expression-System) ...
-
No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
The linear optical properties of the GRAB peptide sensors expressed in HEK293T cells were measured using a Safire 2 plate reader (Tecan). Cells were harvested and transferred to black-wall 384-well plates containing either saline alone or saline containing the corresponding peptides ...
-
No products found
because this supplier's products are not listed.
Bibekananda Sahoo, et al.,
bioRxiv - Immunology 2023
Quote:
... The bound proteins were then reconstituted on-column into peptidisc22 or amphipol by incubation in 5ml of washing buffer supplemented with 10mg peptidisc peptide (Peptidisc Lab) or amphipol A8-35 (Anatrace) for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Xue Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
-
No products found
because this supplier's products are not listed.
Yihong Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Culture medium containing 2 % supplements and different concentration of FOL-026 peptide was added and images recorded with an inverted microscope (Nikon Eclipse TE2000-U ...
-
No products found
because this supplier's products are not listed.
Jennifer E. Wu, et al.,
bioRxiv - Immunology 2023
Quote:
... P14 transgenic mice expressing a TCR specific for the LCMV peptide DbGP33-41 were bred in-house and backcrossed onto the C57BL/6 background (Charles River); recipient WT C57BL/6 mice were purchased from Charles River ...
-
No products found
because this supplier's products are not listed.
Yihong Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Culture medium containing 2 % supplements and different concentration of FOL-026 peptide was added and images recorded with an inverted microscope (Nikon Eclipse TE2000-U, Kawasaki, Japan or Olympus CKX53, Tokyo, Japan) at different time-points ...
-
No products found
because this supplier's products are not listed.
Duy Lan Huong Bui, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
-
No products found
because this supplier's products are not listed.
Tiesuo Zhao, et al.,
bioRxiv - Immunology 2024
Quote:
The tissue samples were incubated with primary antibodies (CD3, 1:100, OmnimAbs; CD4, 1:200, CST; CD8, 1:800, CST; Granzyme B, 1:200, Bioworld; CD86 ...
-
No products found
because this supplier's products are not listed.
Charles R. Heller, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 - 4 tungsten micro-electrodes (FHC, 1-5 MΩ) were inserted to characterize the tuning and response latency of the region of cortex ...
-
No products found
because this supplier's products are not listed.
Marlene Corbet, et al.,
bioRxiv - Pathology 2020
Quote:
DBA/1 (Envigo) mice were maintained in the Central Research Facility of the University of Glasgow and all studies were approved by and performed in accordance with UK Home Office regulations and guidelines (Licences PPL60/26532 ...
-
No products found
because this supplier's products are not listed.
Safiye Aktas, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Math-1 (Bioss), and calretinin (Bioss ...
-
No products found
because this supplier's products are not listed.
Yiran Li, et al.,
bioRxiv - Genomics 2020
Quote:
... 2 □1 water and 5□1 MyTaq™ HS Mix (Bioline). RT-PCR was performed in the following conditions ...
-
No products found
because this supplier's products are not listed.
Sodai Yoshimura, et al.,
bioRxiv - Neuroscience 2024
Quote:
... with an 1× objective (EC Plan-Neofluar 1×/0.025 M27, Carl Zeiss, Jena, Germany). The infarct area was quantified using the AxioVision Rel 4.8 software (Carl Zeiss ...
-
No products found
because this supplier's products are not listed.
Erika H. Dawson, et al.,
bioRxiv - Animal Behavior and Cognition 2024
Quote:
... 1 mm zirconia (BioSpec Products) and approx ...