-
No products found
because this supplier's products are not listed.
Monique Liebers, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 28 ng/μL fish sperm DNA (Roche) and 3 mM TCEP) ...
-
No products found
because this supplier's products are not listed.
Róisín Fattorini, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 2 µl 100 ng/µl fish sperm DNA (Sigma-Aldrich), 1 µl Cy3-dCTP labelled oligos ...
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No products found
because this supplier's products are not listed.
Sarah Lecinski, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1mg/ml salmon sperm DNA solution (UltraPure DNA salmon sperm, ThermoFisher)) ...
-
No products found
because this supplier's products are not listed.
Jonathan D. Hibshman, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Salmon sperm DNA (Agilent technologies, Cat #201190) was boiled for 5 min and then stored on ice ...
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No products found
because this supplier's products are not listed.
Justin E. Silpe, et al.,
bioRxiv - Microbiology 2021
Quote:
... herring sperm DNA (Promega) was used ...
-
No products found
because this supplier's products are not listed.
Teisha J. Rowland, et al.,
bioRxiv - Cancer Biology 2019
Quote:
DNA FISH (Empire Genomics, #TERT-20-OR) of all cell lines and karyotyping of LN-18 cells was performed by the WiCell Research Institute Characterization Laboratory.
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No products found
because this supplier's products are not listed.
Kazutoshi Yoshitake, et al.,
bioRxiv - Genomics 2020
Quote:
... DNA extraction from the liver or fin and pectoral fin muscles of the fish from which sperm was collected were performed using DNeasy Blood & Tissue Kit (QIAGEN).
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No products found
because this supplier's products are not listed.
Rutger A.F. Gjaltema, et al.,
bioRxiv - Genomics 2021
Quote:
RNA FISH was performed using Stellaris FISH probes (Biosearch Technologies). Probe details can be found in Supplementary Table 6 ...
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No products found
because this supplier's products are not listed.
Jakob Rupert, et al.,
bioRxiv - Biochemistry 2023
Quote:
... total DNA from herring sperm (Sigma Merck) was resuspended in sterile ...
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No products found
because this supplier's products are not listed.
Thomas C. Nelson, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Genomic DNA from each fish was digested with PstI-HF (New England Biolabs) and ligated to Illumina P1 adaptors with 6 bp inline barcodes ...
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No products found
because this supplier's products are not listed.
Rachel Newcomb, Emily Dean, James V. Alvarez,
bioRxiv - Cancer Biology 2021
Quote:
... FISH was performed using Kreatech FISH probes (Leica Biosystems) for regions of chromosomes 2 and 11 (KI-30501 ...
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No products found
because this supplier's products are not listed.
Luciana I. Gómez Acuña, et al.,
bioRxiv - Genomics 2023
Quote:
... DNA FISH measurements were made using the quantitation module of Volocity (PerkinElmer) and only alleles with single probe signals were analysed to eliminate the possibility of measuring sister chromatids.
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No products found
because this supplier's products are not listed.
Melanie Balbach, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... with a 10 x objective (mouse sperm) and a 20 x objective (human sperm) (UPLSAPO, NA 0.8; Olympus) was combined with a high-speed camera (ORCA Fusion ...
-
No products found
because this supplier's products are not listed.
Bhawana Shrestha, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The sperm pellet was then washed twice with the sperm washing medium (Irvine Scientific) by centrifugation at 300 g for 10 min ...
-
No products found
because this supplier's products are not listed.
Laura Werel, et al.,
bioRxiv - Microbiology 2022
Quote:
... 850 ng sonicated salmon sperm DNA (GE Healthcare), 1 μg of BSA (Sigma ...
-
No products found
because this supplier's products are not listed.
Yijie Geng, et al.,
bioRxiv - Animal Behavior and Cognition 2021
Quote:
... Genomic DNA extracted from whole fish following Fishbook assays were purified (Zymo Research) before PCR to remove PCR inhibitors in the fish tissues.
-
No products found
because this supplier's products are not listed.
Jose Luis Ros-Santaella, et al.,
bioRxiv - Cell Biology 2023
Quote:
... using a Computer Assisted Sperm Analysis (CASA) (NIS-Elements, Nikon, Tokyo ...
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No products found
because this supplier's products are not listed.
Kohei Nagao, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Immunofluorescence and DNA FISH images were captured using an Axio Imager.A2 microscope (Carl Zeiss) equipped with a CCD camera (Carl Zeiss ...
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No products found
because this supplier's products are not listed.
Ryan H Miller, et al.,
bioRxiv - Genomics 2022
Quote:
... The bisulfite converted sperm DNA was hybridized to Illumina Infinium HumanMethylationEPIC microarrays and ran as recommended by the manufacturer (Illumina) at Infinity BiologiX.
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No products found
because this supplier's products are not listed.
Tamara Maiuri, et al.,
bioRxiv - Neuroscience 2022
Quote:
35 nM PARP1 was incubated with 5 μg/mL sonicated salmon sperm DNA (Abcam ab229278) in deposition buffer (12.5 mM HEPES pH 8 ...
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No products found
because this supplier's products are not listed.
Benjamin H. Glass, et al.,
bioRxiv - Physiology 2022
Quote:
Sperm were concentrated by pipetting 2 mL sperm water into a microcentrifuge tube (Eppendorf), followed by centrifugation at 1500 x g for 5 minutes ...
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No products found
because this supplier's products are not listed.
LiangYu Zhao, et al.,
bioRxiv - Genomics 2020
Quote:
... DNA-FISH was performed according to the guidelines of the CEP X SpectrumOrange/Y SpectrumGreen DNA Probe Kit (Abbott, 07J20-050 and 07J20-050). In brief ...
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No products found
because this supplier's products are not listed.
Pabitra K. Parua, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 0.25 mg/ml salmon sperm DNA (Trevigen) was added ...
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No products found
because this supplier's products are not listed.
Zixin Zhang, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Salmon sperm DNA carrying pGBKT7-CmSVP (BD-CmSVP), pGBKT7-VP16-CmSVP (VP16-CmSVP) ...
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No products found
because this supplier's products are not listed.
Gaku Mizuno, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Polymerase chain reaction (PCR) was performed using the primer set Fish-F1 and Fish-R229 and Tks Gflex DNA polymerase (Takara, Japan). The PCR product was directly sequenced at Macrogen Inc ...
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No products found
because this supplier's products are not listed.
Jana Muroňová, et al.,
bioRxiv - Genetics 2023
Quote:
... Whole sperm or sperm flagella were incubated in 2X Laemmli buffer (Bio-Rad), heated at 95 °C for 10 min and centrifuged (15 000 g ...
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No products found
because this supplier's products are not listed.
Benjamin M. Gansemer, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Blots were blocked with fish gelatin (Biotium, #22010) in TBS for 1 h at RT and then probed with anti-C3 (1:2000 ...
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No products found
because this supplier's products are not listed.
Elsa Leitão, et al.,
bioRxiv - Genetics 2020
Quote:
The DNA isolation was performed on the swim up purified sperm using the MasterPure DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA) as previously described [13] ...
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No products found
because this supplier's products are not listed.
Mark E. Warchol, Angela Schrader, Lavinia Sheets,
bioRxiv - Neuroscience 2020
Quote:
... Larval fish were placed in 25 mm ‘baskets’ (Corning Cell Strainer, ~20-30 fish/basket) and transferred into 30 ml EM that contained 100 μM neomycin (Sigma) ...
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No products found
because this supplier's products are not listed.
Simon Schneider, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Sperm pellets were dissolved in RIPA buffer (Cell Signaling Technology) (10 µl per 1×106 sperm ...
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No products found
because this supplier's products are not listed.
Clémence Fraslin, et al.,
bioRxiv - Genomics 2020
Quote:
Pieces of caudal fin sampled from those 1,152 fish were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for DNA extraction using the DNAdavance kit from Beckman Coulter following manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
DC Bersten, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Electrophoretic mobility shift assays were performed with different competitor DNA (ssDNA-salmon sperm DNA) conditions than SELEX-seq using Fam-labled dsDNA probes were generated by annealing upper fam labeled oligos (IDT DNA, Supplementary table 1). Briefly ...
-
No products found
because this supplier's products are not listed.
Wayo Matsushima, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... sperm samples were resuspended in 1000 μl TriSURE (Bioline) and further homogenised by passing through a syringe (26G ...
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No products found
because this supplier's products are not listed.
Jennifer D. Gresham, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... individual fish were held in 473 mL white plastic cups with lids (VWR Multipurpose Containers ...
-
No products found
because this supplier's products are not listed.
Etienne Lelièvre, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... sibling and eng−/− fish at specific ages as pool using Nucleospin RNA kit (Macherey-Nagel) or NucleoZOL (Macherey-Nagel ...
-
No products found
because this supplier's products are not listed.
Lukas Ded, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Sperm cells were mounted (Vectashield, Vector Laboratories) and imaged with confocal microscopes (Zeiss LSM710 Elyra P1 and Olympus Fluoview 1000).
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No products found
because this supplier's products are not listed.
Federica Zacchini, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... sperm fraction was diluted 1:10 in Phosphate Buffered Saline (PBS, Lonza) and CellROX reagents (1 μM/μl ...
-
No products found
because this supplier's products are not listed.
Fatma Pir Cakmak, et al.,
bioRxiv - Biophysics 2021
Quote:
Protamine sulfate from salmon sperm was acquired from MP Biomedicals (Santa Ana, CA) or Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Mélina Blanco, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Sperm cells were sonicated (ON 30sec – OFF 30sec x13 cycles, Bioruptor Pico, Diagenode) and the sheared chromatin was centrifuged at 20 000 x g for 10 min at 4°C ...
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No products found
because this supplier's products are not listed.
Rozhin Penjweini, et al.,
bioRxiv - Cell Biology 2023
Quote:
... pMyo-mCherry was prepared by cloning the myoglobin gene from Physeter catodon (Sperm Whale, Addgene Plasmid pMB413a, #20058) into the pmCherry N1 vector (Clonetech ...
-
No products found
because this supplier's products are not listed.
Xuan T.A. Nguyen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... XM_003200755.5), medaka fish (MeMfsd7c, XM_004082328.4), and frog Mfsd7c (XeMfsd7c, NM_001016982.2) coding sequences were synthesized by GenScript and cloned into pcDNA3.1 plasmid for overexpression ...
-
No products found
because this supplier's products are not listed.
Yuechao Wu, et al.,
bioRxiv - Genetics 2022
Quote:
... Genomic DNA was extracted using Plant Genome DNA Kit (Tiangen). All plant samples were sequenced by the Illumina NovaSeq platform (Novogene ...
-
No products found
because this supplier's products are not listed.
Ryo Yoshimura, et al.,
bioRxiv - Plant Biology 2023
Quote:
... DNA extracted DNA was fragmented by sonication (S220 from Covaris) and then converted to libraries using TruSeq DNA Sample Prep Kits (Illumina ...
-
No products found
because this supplier's products are not listed.
Lydia J. Baker, et al.,
bioRxiv - Microbiology 2021
Quote:
... DNA was extracted using EZNA Tissue DNA Kit (Omega Bio-Tek) and the quality of the extraction was evaluated using the dsDNA HS assay using a Qubit 3.0 Fluorometer ...
-
No products found
because this supplier's products are not listed.
Jie Zhu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Fish were embedded in EMbed-812 (EMS). Hair cells from anterial neuromasts were imaged on Tecnai Biotwin electron microscope.
-
No products found
because this supplier's products are not listed.
Md Ehsanul Haque, Most Shanaj Parvin,
bioRxiv - Plant Biology 2022
Quote:
... Genomic DNAs (Norgen Biotek Corp, Fungi DNA Isolation Kit #26200) (Table B.1. ...
-
No products found
because this supplier's products are not listed.
Pawel Sledzinski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Genomic DNA was extracted using the Genomic DNA Isolation Kit (Norgen, Biotek Corp.) according to the manufacturer’s instructions and quantified using a spectrophotometer/fluorometer (DeNovix) ...
-
No products found
because this supplier's products are not listed.
Aya Abou Hammoud, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Tks5 (fish M-300 sc30122, Santa cruz), Cortactin (clone 4F11 Millipore 5180 ...
-
No products found
because this supplier's products are not listed.
Ryan M. Baxley, et al.,
bioRxiv - Molecular Biology 2020
Quote:
FISH was performed with TelC-Cy3 (PNA Bio F1002) and CENPB-Alexa488 (F3004 ...
-
No products found
because this supplier's products are not listed.
Hao Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
... DNA/PEI (Polysciences) complexes were suspended in Opti-MEM medium (Gibco ...