-
No products found
because this supplier's products are not listed.
Alyssa N Coyne, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Antigen retrieval was performed using Tissue-Tek antigen retrieval solution (IHC World) for 1 hour in a steamer ...
-
No products found
because this supplier's products are not listed.
Deivys Leandro Portuondo Fuentes, et al.,
bioRxiv - Immunology 2019
Quote:
... diluted 1:500 or goat anti-feline IgG (Southern Biotech) diluted 1:1000 ...
-
No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
-
No products found
because this supplier's products are not listed.
Kim S. Friedmann, et al.,
bioRxiv - Immunology 2020
Quote:
... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
-
No products found
because this supplier's products are not listed.
Manutsanun Inthawong, et al.,
bioRxiv - Immunology 2022
Quote:
... A T cell antigen control pool (CEF, Mabtech AB, 3616-1) at a final concentration of 1 μg/ml was used as a source of control antigens ...
-
No products found
because this supplier's products are not listed.
Zeqing Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Antigen retrieval was performed with Antigen Retrieval Solution (Solarbio, C1035) before the conventional immunostaining procedure.
-
No products found
because this supplier's products are not listed.
Andrea R. Shiakolas, et al.,
bioRxiv - Immunology 2021
Quote:
... AviTagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
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No products found
Adam K. Wade-Vallance, et al.,
bioRxiv - Immunology 2022
Quote:
... Biosearch Technologies]) or control antigen (ovalbumin; OVA [Worthington Biochemicals]) in 20µl PBS by subcutaneous injection ...
-
No products found
because this supplier's products are not listed.
Jianlong Zhai, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the antigen-antibody complexes were detected using Odyssey XF (LI-COR, USA). The signal intensities of target bands were analyzed using ImageJ software (National Institutes of Health ...
-
No products found
because this supplier's products are not listed.
W.J. Venema, et al.,
bioRxiv - Immunology 2021
Quote:
... S-antigen (α-mGFP, TA180076, Origene, to detect the fusion protein S-antigen-GFP ...
-
No products found
because this supplier's products are not listed.
Jesse M. Hall, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antigen specific antibody titers to PT (List Biological Laboratories #180) were measured by coating ELISA plates with 50 μl of antigen per well ...
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No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
because this supplier's products are not listed.
Ravi Ojha, et al.,
bioRxiv - Microbiology 2023
Quote:
... For CPV infections, MEF cells was transfected with feline TfR1 plasmid (pCTfR, 2.5 μg)25 by using TransIT-LT1transfection reagent (Mirus Bio, WI, USA). To generate MEFDyn1,2,3 TKO cells stably expressing hACE2 ...
-
No products found
because this supplier's products are not listed.
Yanzhao Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The p24 antigen levels in viral supernatants were measured by an HIV-1 p24 antigen capture ELISA (XpressBio). Transfection efficiencies were normalized to the luciferase activity ...
-
No products found
because this supplier's products are not listed.
Nuno Apóstolo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1:50 donkey anti-mouse IgG antigen-binding fragments (Jackson ImmunoResearch). Sections were then washed in PBS-0.5%Triton X-100 at RT and incubated at 4°C overnight with primary antibodies in PBS containing 5% NHS ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Paul Castillo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Synthetic mRNA encoding for full-length model antigen (i.e., CleanCap GFP, catalog L-7601 and CleanCap ovalbumin, OVA, catalog L-7610) were purchased from TriLink Biotechnologies (San Diego ...
-
No products found
because this supplier's products are not listed.
BI Reinfeld, et al.,
bioRxiv - Cancer Biology 2020
Quote:
PyMT GEMM mice were bred by crossing male transgenic mice expressing the polyoma virus middle T antigen (PyMT) oncoprotein under the MMTV-LTR (Jackson Laboratory 022974) with wildtype females on a similar B6/FVB mixed background ...
-
No products found
because this supplier's products are not listed.
Tomaz Martini, et al.,
bioRxiv - Neuroscience 2020
Quote:
... antigen retrieval and antibody staining (anti-PER2, Alpha Diagnostic, cat ...
-
No products found
because this supplier's products are not listed.
Caroline E. Dewar, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The antibody was affinity purified against the antigen by Eurogentec, and used at a 1:50 dilution ...
-
No products found
because this supplier's products are not listed.
Teresa R. Wagner, et al.,
bioRxiv - Immunology 2023
Quote:
... purified antigen (Acrobiosystems) was reacted with Sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Federico Giannitti, et al.,
bioRxiv - Pathology 2022
Quote:
... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
-
No products found
because this supplier's products are not listed.
Yuan Zhang, Zhigang Liu, Bin Zhang,
bioRxiv - Cell Biology 2022
Quote:
... FVIII antigen was quantified by ELISA using the VisuLize FVIII antigen kit from Affinity Biologicals (Ancaster, ON, Canada). FV antigen was quantified by ELISA using matched-pair antibody set for human FV antigen from Affinity Biologicals ...
-
No products found
because this supplier's products are not listed.
Francisco Javier Villaamil, et al.,
bioRxiv - Epidemiology 2019
Quote:
... this was confirmed by antigen capture ELISA (IDEXX BVDV Ag Serum Plus ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Md. Alamgir Hossain, et al.,
bioRxiv - Immunology 2022
Quote:
... incubated with each antigen at certain concentration in PBS/2% FCS and transfer to a 37°C thermomixer (Eppendorf). Cells were collected after each period of incubation and fixed in IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Antigen for immunization was prepared by mixing NP-OVA (Biosearch Technologies), 10% KAL(SO4)2 dissolved in PBS at a ratio of 1:1 ...
-
No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
-
No products found
because this supplier's products are not listed.
Wei Deng, et al.,
bioRxiv - Microbiology 2020
Quote:
... an antigen retrieval kit (AR0022, Boster) was applied to the sections at 37°C for 1 min ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Yitzhak Reizel, et al.,
bioRxiv - Genetics 2020
Quote:
... and loaded into a 2100 Antigen Retriever (Proteogenix). After reaching the target temperature ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIV antigen GP120 was purchased from Immune Technology Corporation (IT-001-022p) ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Gururaj Shivange, et al.,
bioRxiv - Biochemistry 2021
Quote:
... For binding analysis biotinylated antigen (1 μg/mL) were immobilized on streptavidin (SA) biosensors (Pall) for 300 sec to ensure saturation ...
-
No products found
because this supplier's products are not listed.
Katrin Manske, et al.,
bioRxiv - Bioengineering 2023
Quote:
20,000 Human Embryonic Kidney cells (HEK293-CD19+) expressing the CD19 antigen were seeded on a 96-well E-plate (ACEA Biosciences) over night ...
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
-
No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
-
No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Yuka Sakata, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Antigens were retrieved using HistoVT One (Nacalai USA) for 20 min at 70 °C and washed in PBS for 10 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
-
No products found
because this supplier's products are not listed.
Jiahn Choi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 3′-diaminobenzidine (DAB) for visualization of the antigen-antibody complex (Scytek).
-
No products found
because this supplier's products are not listed.
Mathijs P. Verhagen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Antigen retrieval was performed in the 2100 Retriever pressure cooker (BioVendor) at pH 9 with Tris-EDTA buffer ...
-
No products found
because this supplier's products are not listed.
Denisa Bojkova, et al.,
bioRxiv - Microbiology 2022
Quote:
... H1N1 (Influenza A Virus) Nucleoprotein (Bioss, #bs-4976R, 1:4000), ISG15 (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
S. Grabherr, et al.,
bioRxiv - Immunology 2022
Quote:
... Antigen-specific T cells were detected using PE-conjugated s598 tetramers (Sanquin Reagents) after an incubation of 20 min at 37 °C ...
-
No products found
because this supplier's products are not listed.
Jakob Kreye, et al.,
bioRxiv - Immunology 2020
Quote:
... The presence of immobilized antigens was confirmed by incubation with HRP-conjugated anti-rabbit IgG (Dianova). Assays for concentration-dependent RBD binding (Fig ...