-
No products found
because this supplier's products are not listed.
Gabrielle L. Turvey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
-
No products found
because this supplier's products are not listed.
Caroline L. Wee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... with a number of core differences: 1) 15 µM instead of 25 µM Optovin (Tocris Biosciences) was used ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
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No products found
Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Avital Licht-Murava, et al.,
bioRxiv - Neuroscience 2022
Quote:
... per ml culture medium at DIV 8 using Lipofectamine 3000 5 h before infection with vesicular stomatitis virus (VSV) at 100 MOI or with adenovirus-eGFP (Ad5CMV-eGFP, lot #ad3586, Viral Vector Core Facility, Carver College of Medicine ...
-
No products found
because this supplier's products are not listed.
Vanessa Brinkmann, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the qPCR Green Core kit (Jena Biosciences, PCR-333L) or the GoTaq® qPCR kit (Promega ...
-
WB, IHC,ELISA
Cat# A5436, SKU# A5436-100ul,
100ul, $157.00
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Mirca S. Saurty-Seerunghen, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and the SYBR Green PCR Core Reagents kit (Bimake.com). The thermal cycling conditions comprised an initial denaturation step at 94°C for 5 min ...
-
No products found
because this supplier's products are not listed.
Liam P. Coyne, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and desalted using 2-core MCX stage tips (3M) (89) ...
-
No products found
because this supplier's products are not listed.
Eveline Ultee, Ariane Briegel, Dennis Claessen,
bioRxiv - Microbiology 2020
Quote:
... 3,5 μl of the culture was mixed with 10 nm gold fiducial markers (Cell Microscopy Core, UMC Utrecht) and applied onto glow-discharged R2/2 200 mesh holey carbon EM grids (Quantifoil). The grid was then rapidly plunged in liquid ethane using the Leica GP automated freeze-plunger ...
-
No products found
because this supplier's products are not listed.
Erin T. Larragoite, et al.,
bioRxiv - Microbiology 2021
Quote:
... Non-adherent PBMCs were then cultured at a density of 1 × 105 cells/100µL in the presence of 100nM compounds from a Cayman Chemical Epigenetics Screening Library obtained from the University of Utah Drug Discovery Core Facility (Item No. 11076) or 100nM ruxolitinib (Cayman Chemical Co., Item No. 11609), a control for cytokine inhibition ...
-
No products found
because this supplier's products are not listed.
Mayumi K. Holly, et al.,
bioRxiv - Microbiology 2020
Quote:
... C-reactive protein (MyBioSource). The optical density was read at 450 nm on an EnVision plate reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
He Liu, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The cytoplasmic and nucleic protein were extracted separately using the protein extraction kit (Boster) according to the manufacture’s introduction ...
-
No products found
because this supplier's products are not listed.
Jingqi Zhou, et al.,
bioRxiv - Genomics 2021
Quote:
Zebrafish’s bone morphogenetic protein (BMP) and bone morphogenetic protein receptor genes were used as query to search the BMP and BMP receptor gene families in P ...
-
No products found
because this supplier's products are not listed.
Elaine C. Chen, et al.,
bioRxiv - Immunology 2021
Quote:
... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Lujie Liang, et al.,
bioRxiv - Microbiology 2023
Quote:
... LPS core (Hycult Biotech) and HA-tag (Cell Signalling ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
-
No products found
because this supplier's products are not listed.
Jiefu Jin, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... from 6 cases of breast invasive ductal carcinoma (two cores per group) with matched adjacent breast tissue (two cores per group) were purchased from US Biomax (Derwood, MD, USA). The TNM stage and grade of 6 cases were TisN0M0 and grade 1 for cores A1 and A2 ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Kevin D. de Young, et al.,
bioRxiv - Microbiology 2019
Quote:
... Samples were sent to the Metabolomics Core Facility of the Cancer Institute of New Jersey (New Brunswick, NJ) for analysis by liquid chromatography/mass-spectrometry (LC/MS) ...
-
No products found
because this supplier's products are not listed.
Justin A. Bosch, et al.,
bioRxiv - Genetics 2019
Quote:
Sanger sequencing was performed at the DF/HCC DNA Resource Core facility and chromatograms were analyzed using Lasergene 13 software (DNASTAR). Next-generation sequencing was performed at the MGH CCIB DNA Core ...
-
No products found
because this supplier's products are not listed.
Aidan Foo, et al.,
bioRxiv - Microbiology 2023
Quote:
... and sent to the UW-Madison Biotechnology Center Next Generation Core facility for library preparation using the Celero EZ DNA-Seq Library Prep kit (Tecan, Männedorf, Switzerland), followed by sequencing on the Illumina NovaSeq6000 platform to generate 150 bp paired end reads.
-
No products found
because this supplier's products are not listed.
Xueyan Zhang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 400 μg total RNA from virus-infected Vero-E6 cells was incubated with an anti-m6A antibody (Synaptic Systems, Goettingen, Germany) or an IgG antibody in 300 μL IP buffer (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Miroslav Homola, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The dialyzed virus particles were concentrated in centrifugal ultrafiltration units to 200 µl and mixed with Turbo Nuclease (Abnova, Taipei, Taiwan) at a final concentration of 25 U ml-1 to digest free nucleic acids released into solution ...
-
No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
-
No products found
because this supplier's products are not listed.
Han Liao, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... La protein (A303-901A, Bethyl), RUVBL1 (A304-716A ...
-
No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
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No products found
because this supplier's products are not listed.
Li Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... The expression of nucleocapsid protein was confirmed by the SARS-CoV-2 Nucleocapsid Protein ELISA Kit (ABclonal, Woburn, MA) and the cell clone supporting the most efficient virus rescue was selected (VeroE6-N) ...
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With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
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Ethan L. Morgan, et al.,
bioRxiv - Microbiology 2020
Quote:
... Proteins were detected using WesternBright ECL (Advansta, USA) and visualised on X-ray film.
-
No products found
because this supplier's products are not listed.
Kun Wang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... To reconstitute MBOAT7 proteins in PMAL-C8 (Anatrace), MBOAT7 in detergent was mixed with PMAL-C8 at a 1:3 (w:w ...
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No products found
because this supplier's products are not listed.
Zhesi Zhu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... with gentle sonication and core histone proteins were extracted with the EpiQuik Total Histone Extraction Kit (Epigentek, Cat# OP-0006-100). The extracted lysates and histone proteins were resolved on a 4-20% gradient gel (Biorad ...
-
No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ...
-
No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
-
No products found
because this supplier's products are not listed.
Sophie E. Cousineau, et al.,
bioRxiv - Microbiology 2022
Quote:
... mouse anti-HCV core (clone B2, Anogen MO-I40015B, 1:7,500); mouse anti-JFH-1 NS5A (clone 7B5 ...
-
No products found
because this supplier's products are not listed.
Sarah Richtmann, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... TMA cores were annotated using the TMA function of the Phenochart software (Akoya Biosciences) by setting a grid with 1.2Lmm punch diameter.
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
... A fixed concentration of SARS-CoV-2 GFP pseudotyped virus (BPS Biosciences) was added and the plate incubated for 60 minutes at 37°C and 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Hanna Jérôme, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... purified virus RNA was treated with 5 units of tobacco acid pyrophosphatase (Epicentre) to generate 5’ monophosphorylated termini ...
-
No products found
because this supplier's products are not listed.
CV Giuraniuc, et al.,
bioRxiv - Microbiology 2023
Quote:
... A DeltaVision Core microscope (Image Solutions Ltd., UK) with a CoolSNAP camera (Photometrics United Kingdom Ltd., UK), a 60× objective and a GFP filter was used for live-cell imaging ...
-
No products found
because this supplier's products are not listed.
Rajagopal Ayana, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
-
No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
-
No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
-
No products found
because this supplier's products are not listed.
Ourania Galanopoulou, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... or C1s proteins (Complement Technologies), or BSA (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Yutong Song, et al.,
bioRxiv - Biophysics 2022
Quote:
... The protein band was stained by One-Step Blue Protein Gel Stain (Biotium, Fremont, CA).
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... or ERK3 protein (M31-34G, SignalChem). ARP2/3 protein complex ...
-
No products found
because this supplier's products are not listed.
Lien D. Nguyen, et al.,
bioRxiv - Neuroscience 2022
Quote:
Total protein was extracted using RIPA buffer (Boston Bioproducts) supplemented with Complete ...
-
No products found
because this supplier's products are not listed.
Lijun Cao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The purified protein was digested with PreScission Protease (APEXBIO) to remove GST tag and treated with 25 mM DTT or 50 μM H2O2 at 25 °C for 30 min ...
-
No products found
because this supplier's products are not listed.
Jordi Lambert, et al.,
bioRxiv - Cell Biology 2019
Quote:
... biotinylated protein was isolated by immunoprecipitation using anti-biotin (Jackson Immunoresearch), and levels of α5 integrin assessed by SDS-PAGE.