-
No products found
because this supplier's products are not listed.
Matthew Prideaux, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Antibody against fatty acid binding protein 4 (FABP4) was purchased from ProSci, Inc ...
-
No products found
because this supplier's products are not listed.
Maude Giroud, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... triglycerides and non-esterified fatty acids using a serum analyzer (AU480 Chemistry analyzer Beckman Coulter). Plasma levels of leptin and insulin were assessed using ELISAs used according to the manufacturer’s instructions (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Gautier Langin, et al.,
bioRxiv - Plant Biology 2023
Quote:
... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
-
No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ying Li, et al.,
bioRxiv - Cell Biology 2020
Quote:
... BODIPY-dodecanoic acid fluorescent fatty acid analog was added (QBT™ Fatty Acid Uptake Assay Kit, Molecular Devices) and the plate was immediately transferred to a fluorescence microplate reader for kinetic reading (every 20 seconds for 30-60 minutes ...
-
No products found
because this supplier's products are not listed.
Nicole D. Hryckowian, et al.,
bioRxiv - Microbiology 2024
Quote:
... Fatty acids were purchased from Nu-Chek Prep (linoleic acid ...
-
No products found
because this supplier's products are not listed.
Bo Peng, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Fatty acid synthase (FAS) inhibitor cerulenin was purchased from Enzo life sciences (USA).
-
No products found
because this supplier's products are not listed.
Xin-Hua Liu, et al.,
bioRxiv - Physiology 2021
Quote:
... Alanine aminotransferase (ALT) and free fatty acid (FFA) assay kits were purchased from MyBioSource Inc ...
-
No products found
because this supplier's products are not listed.
Ksenija Radic Shechter, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 pmol lactosylceramide (d18:1 with N-acylated C17 fatty acid, Avanti Polar Lipids), 10 pmol phosphatidic acid (21:0/22:6 ...
-
No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
PMA-differentiated THP1 macrophages in 24-well plates were pretreated with fatty acids (2.5 μM and 10 μM) for 30 min prior to stimulation with 4 μg/mL cGAMP (InvivoGen) using Lipofectamine 2000 (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Nishant Mehta, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Binding of VISTA was detected indirectly by first adding 1:750 rabbit anti-6-HIS (Bethyl, A190-114F) and then adding 1:7500 anti-rabbit-HRP (Novus Biologicals ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Jeremy R. Egbert, et al.,
bioRxiv - Cell Biology 2019
Quote:
... antibody binding was detected using fluorescent secondary antibodies (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Wenwen Tang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mice were placed on an essential fatty acid-free Diet (TD.84224, Envigo) for 4 days ...
-
No products found
because this supplier's products are not listed.
Kathryn M. Spitler, et al.,
bioRxiv - Physiology 2020
Quote:
... Non-esterified fatty acid concentrations were determined using a standard curve prepared from Oleic Acid standard (TCI, O0180, Lot Z8RVM). Fasting plasma glucose concentration was measured once per mouse immediately following a tail nick using a glucometer (One Touch Ultra) ...
-
No products found
because this supplier's products are not listed.
Kaito Nagashima, et al.,
bioRxiv - Immunology 2021
Quote:
... AviTagged Y2 COBRA HA proteins containing the Y98F mutation to reduce sialic acid binding were biotinylated using the BirA biotin-protein ligase in the BirA500 kit (Avidity) and complexed to streptavidin-fluorophores SA-PE (1:500 dilution ...
-
No products found
because this supplier's products are not listed.
Julia Rogal, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The uptake of the fatty acids was monitored in real-time using a fluorescence microscope with incubation (Leica DMi8 with incubation unit ...
-
No products found
because this supplier's products are not listed.
Hoang V. Dinh, et al.,
bioRxiv - Systems Biology 2019
Quote:
... 20nM isotope-labeled fatty acid standards (U-13C-C16:0, U-13C-C18:1, U-13C-C18:2; Cambridge Isotope) were added before saponification as internal standards ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Leah Cuthbertson, et al.,
bioRxiv - Microbiology 2022
Quote:
... holo (human) (10 μg/ml) & retinoic acid (0.1 ng/ml) (Promocell, Germany) and Primocin (Invivogen ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Yann Waltenspühl, et al.,
bioRxiv - Biochemistry 2022
Quote:
Ligand-induced IP1 accumulation and ligand-binding experiments were measured using transiently transfected Human Embryonic Kidney (HEK) 293T/17 cells (American Type Culture Collection). The cells were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Sonwabile Dzanibe, et al.,
bioRxiv - Immunology 2021
Quote:
... 15 and 36 weeks of age were used to measure the concentration of intestinal fatty-acid binding protein (iFABP) using a commercial ELISA kit (Elabscience). Test samples were diluted 1/400 in assay diluent and measured in duplicates according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Luke Izzo, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Fatty acid supplementation used palmitate and/or oleate conjugated to fatty acid free BSA (Bioworld 22070023) and fatty acid free BSA in an equivalent volume was supplemented as control.
-
No products found
because this supplier's products are not listed.
Albert Pérez-Martí, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The Monounsaturated Fatty Acid-High Fat Diet (MUFA-HFD) and Saturated Fatty Acid-High Fat Diet (SFA-HFD) were obtained from Research Diets. The HFDs (Research Diets ...
-
No products found
because this supplier's products are not listed.
Kuan-Yi Lu, et al.,
bioRxiv - Microbiology 2020
Quote:
... 3% fatty acid-free BSA) was probed to preblocked PIP strips (Echelon Biosciences) and incubated at 4 °C for 16 h ...
-
No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
-
No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
-
No products found
because this supplier's products are not listed.
Akos Nyerges, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... and the supernatant was mixed in a 1:1 ratio with HIS-Binding/Wash Buffer (G-Biosciences, USA) and 50 µl HisTag Dynabeads (Thermo Fischer Scientific ...
-
No products found
because this supplier's products are not listed.
Solveigh C. Koeberle, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
Glycerophospholipids were detected by multiple reaction monitoring in the negative ion mode based on their fatty acid anion fragments using a QTRAP 5500196 or QTRAP 6500+ Mass Spectrometer (Sciex), which were equipped with electrospray ionization (ESI ...
-
No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Leandre M. Glendenning, et al.,
bioRxiv - Immunology 2023
Quote:
Sialic acids were removed from human polyclonal IgG (Innovative Research IHIUGGAP) via recombinant neuraminidase (NEB P0720L ...
-
No products found
because this supplier's products are not listed.
Leslie E. Lupien, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... human DiI-VLDLs (1 mg protein/mL; Alfa Aesar Chemicals), LPL from bovine milk (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
-
No products found
because this supplier's products are not listed.
Goonho Park, et al.,
bioRxiv - Neuroscience 2022
Quote:
... harboring T34 isoform of microtubule-associated protein tau with one N-terminal insert and four microtubule binding repeats (1N4R) encoding the human P301S mutation were obtained from Jackson Laboratory and maintained in a C57Bl/6j genetic background in standard vivarium environment (12h light:12h dark cycle) ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Yining Liu, et al.,
bioRxiv - Bioengineering 2023
Quote:
... they were stained in Phosphomolybdic Acid-Phosphotungstic Acid (EMS) for 15 minutes and then in Aniline Blue (EMS ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Human primary astrocytes (ScienCell) were cultivated as recommended by ScienCell ...
-
No products found
because this supplier's products are not listed.
Firyal Ramzan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkyne-tagged fatty acid analogs (alkyne-myristate (13-tetradecylnoic acid; 13-TDYA; Click Chemistry Tools 1164) or alkyne-palmitate (15-hexadecynoic acid ...
-
No products found
because this supplier's products are not listed.
Yilie Liao, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Fatty acids from total lipids were released by saponification (refluxing with 3mL 0.3M KOH in 90% methanol at 80°C for 1 hour and remaining ∼300μL solvent) ...
-
No products found
because this supplier's products are not listed.
Fransky Hantelys, et al.,
bioRxiv - Cell Biology 2019
Quote:
Binding analyses were performed with recombinant protein VASH1 (Abnova H00022846-P01) at 100 µg/ml over the immobilized FGF1 ...
-
No products found
because this supplier's products are not listed.
Katarzyna Bogucka-Janczi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human recombinant GST-fusion ERK3 protein (SignalChem) and E.coli purified ARP3 ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Purified human complement proteins (4 µg/mL, Complement Technology) were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL ...
-
No products found
because this supplier's products are not listed.
Tao Guo, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The fusion proteins were detected with HRP-conjugated mouse anti-His monoclonal antibody (ABclonal, AE028, 1:3000 dilution) and visualized by enhanced chemiluminescence.
-
No products found
because this supplier's products are not listed.
Nathaniel Felbinger, et al.,
bioRxiv - Immunology 2022
Quote:
... slides were again aspirated and probed with a mixture of fluorescent secondary antibodies that would be used for peptide binding detection consisting of Rabbit Anti-Human IgG DyLight™ 800 (Rockland Immunochemicals), Alexa Conjugated Goat Anti-Human IgA 680 (Jackson Immuno Research Labs ...
-
No products found
because this supplier's products are not listed.
Jean-Philippe Guégan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif) according to manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Ella N. Hoogenboezem, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Nucleic acid was visualized with GelRed Nucleic Acid Stain (Biotium) under ultraviolet imaging and protein visualized by Coomassie Blue stain under visible light imaging.
-
No products found
because this supplier's products are not listed.
Sanjib Saha, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein expression was normalized to total protein (DM-TP01, Protein Simple) and compared between cell types.