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No products found
because this supplier's products are not listed.
Inês Caldeira Brás, et al.,
bioRxiv - Neuroscience 2021
Quote:
Human embryonic kidney (HEK) 293 cells (ATTC, VA, USA) were maintained in DMEM medium (PAN Biotech, Aidenbach, Germany) supplemented with 10% FBS (Anprotec ...
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No products found
because this supplier's products are not listed.
Juan A. Ortega, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Image acquisition for HEK-293 experiments was performed on a Leica DMI4000B laser scanning confocal microscope (Leica, Buffalo Grove, IL) or with Leica DMi8 microscope (Leica ...
-
No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
-
No products found
because this supplier's products are not listed.
Cheng-Tsung Pan, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... A mouse 6X His tag antibody (HRP) (GeneTex Inc.) was then used to recognize Gal3-His at room temperature for 1 hour ...
-
No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Vishant Boradia, et al.,
bioRxiv - Microbiology 2022
Quote:
... Blots were probed with mouse α-His antibody followed by IRDye® 680RD Goat α-Mouse IgG Secondary Antibody (LI-COR) to detect the His tagged PE15 ...
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No products found
because this supplier's products are not listed.
Katarzyna Szymanska, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and RNF34-HIS (Novus Biologicals) fusion proteins in a total volume of 30μl ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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No products found
because this supplier's products are not listed.
Cristina Lao-Peregrin, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK 293 cells were purchased from ATCC (CRL-1573) and authenticated by Bio-Synthesis, Inc ...
-
No products found
because this supplier's products are not listed.
Claudia E. Varela, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 3μg of FSTL1 (Aviscera Biosciences, suspended in 30 μl of sterile PBS) were injected through the port into the refill line followed by 20 μl of PBS to clear the refill line from any FSTL1 and ensure the FSTL1 reached the epicardial reservoir.
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No products found
because this supplier's products are not listed.
Raphael Fettig, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NIH-3T3 fibroblasts and HEK-293 cells (all from ATCC, LGC Standards GmbH, Wesel, Germany) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
-
No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
HEK-NF-κB_luc reporter cells (BPS Bioscience, USA) were transfected with bacterial ETLs or in vitro-synthesized heptose metabolites using Lipofectamine 2000 transfection agent (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Rose Blake, et al.,
bioRxiv - Microbiology 2023
Quote:
... anti-His monoclonal antibody (Raybiotech) and goat anti-rabbit IgG were used as primary and secondary antibodies (Supplementary Table 1) ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
-
No products found
because this supplier's products are not listed.
Vi Pham, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and HEK 293T cells were replenished with mTeSR™ Plus medium (StemCell Technologies). After 48 hours ...
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No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Ava P. Soleimany, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Hi-Myc mice (FVB-Tg(ARR2/Pbsn-MYC)7Key/Nci) were obtained from Jackson Labs. The Hi-Myc model was generated as previously described (35) ...
-
No products found
because this supplier's products are not listed.
Alessio D. Nahmad, et al.,
bioRxiv - Immunology 2020
Quote:
... or anti-mouse IgG or anti-mouse IgG1 or anti-mouse IgM (Jackson ImmunoResearch) at 2μg/ml in PBST and were incubated for two hours ...
-
No products found
because this supplier's products are not listed.
Camila de Britto Pará de Aragão, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mouse anti-mouse Gephyrin (1:1000, Synaptic Systems, catalog #147021), Neuroligin-1 (1:300 ...
-
No products found
because this supplier's products are not listed.
Sandra Wimberger, et al.,
bioRxiv - Molecular Biology 2022
Quote:
HEK293T cells (parental clone HEK-293, ATCC CRL-1573; Genhunter corporation Q401) and HEK293T-KO cell pools (PARP1-/- ...
-
No products found
because this supplier's products are not listed.
Juan A. Ortega, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 40% confluent HEK-293 cells were transfected with HilyMax transfection reagent (Dojindo Molecular Technologies) according to manufacturer guidelines ...
-
No products found
because this supplier's products are not listed.
Maria B. Campaña, et al.,
bioRxiv - Biochemistry 2023
Quote:
Mammalian cell line HEK 293 (ATCC) was maintained in 75 mL vented tissue culture flasks (Celltreat) and kept to 70% confluency before splitting ...
-
No products found
because this supplier's products are not listed.
Magda Krakowczyk, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HEK-293 cells (60%-70% of confluency) were fixed by 2.5% glutaraldehyde and 2% paraformaldehyde (both from Electron Microscopy Science, Inc.) solution for 1 h at 4°C ...
-
No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
-
No products found
because this supplier's products are not listed.
Maxime Lorenzini, et al.,
bioRxiv - Physiology 2020
Quote:
Whole-cell NaV currents were recorded at room temperature from transiently transfected HEK-293 cells using an Axopatch 200A amplifier (Axon Instruments, Molecular Devices, San Jose, CA) 48 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Nikhil J. Parekh, et al.,
bioRxiv - Immunology 2019
Quote:
IFN-β protein was detected using the mouse Hi-Sensitivity IFN-β ELISA kit (PBL Assay Science). CCL4 protein was detected using the mouse CCL4/MIP-1 beta Quantikine ELISA kit (R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Ruud H. Wijdeven, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
-
No products found
because this supplier's products are not listed.
Liisa Nissinen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK and HOK (human oral keratinocytes) were from ScienCell Research Laboratories (Carlsbad ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
-
No products found
because this supplier's products are not listed.
Bekir Altas, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
-
No products found
because this supplier's products are not listed.
Luyao Huang, et al.,
bioRxiv - Plant Biology 2023
Quote:
... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
No products found
because this supplier's products are not listed.
Constanza Feliziani, et al.,
bioRxiv - Cell Biology 2020
Quote:
Triple IP3R knockout human embryonic kidney cell line (TKO-HEK) from Kerafast (Boston, MA, USA; #EUR030) was grown in DMEM (#11995-065 ...
-
No products found
because this supplier's products are not listed.
Heng Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the immobilized FLAG-NBR1 was incubated with 1.5 μg His-MAPT and 1 μg GST or GST-BAG3 (Abnova H00009531-P01) in the BAG buffer (25 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Tara A. Gleeson, et al.,
bioRxiv - Immunology 2024
Quote:
... mouse-anti-mouse NLRP3 (1/1000 dilution; Cryo2, Adipogen, AG-20B-0014), rabbit-anti-mouse gasdermin-D (1/1000 dilution ...
-
No products found
because this supplier's products are not listed.
Maitreyi Rathod, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse DSG1 ((#651111 Progen), rabbit keratin 10 (#905403 Biolegend) ...
-
No products found
because this supplier's products are not listed.
Mitsugu Shimobayashi, et al.,
bioRxiv - Physiology 2020
Quote:
Plasma Leptin levels were measured by mouse mouse Leptin ELISA kit (Crystal Chem) according to the manufacturer’s instructions.