-
No products found
because this supplier's products are not listed.
Nathan J. Dwarshuis, et al.,
bioRxiv - Bioengineering 2019
Quote:
The anti-CD19-CD8-CD137-CD3z chimeric antigen receptor with the EF1α promotor [28] was synthesized (Aldevron) and subcloned into a FUGW lentiviral transfer plasmid (Emory Viral Vector Core) ...
-
No products found
because this supplier's products are not listed.
Lorna Ewart, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Cryopreserved human Kupffer cells (Samsara Sciences) and human Stellate cells (IXCells) were thawed according to their respective vendor/Emulate protocols on the day of seeding ...
-
No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
-
No products found
because this supplier's products are not listed.
Neil J Rzechorzek, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HEK293-F cells were grown in 400 mL batches in 2 L non-baffled Erlenmeyer flasks (TriForest Enterprises Inc, #FPC2000S) to a cell density of ∼1×106 cells/mL.
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No products found
because this supplier's products are not listed.
Justine Creff, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
-
No products found
because this supplier's products are not listed.
Anne Rehkamp, et al.,
bioRxiv - Biochemistry 2020
Quote:
LC separation of peptides originating from HEK293 cell membranes was performed via selfpacked C18 columns (PicoFrits, 75 μm x 50 cm, 15 μm tip, New Objective, self-packed with ReproSil-Pur 120 C18-AQ ...
-
No products found
because this supplier's products are not listed.
Ronan Shaughnessy, et al.,
bioRxiv - Cell Biology 2023
Quote:
... lentivi-ral particles were produced in the HEK293 FT packaging cells transfected with MiniBAR constructs and the Excelenti LTX Lentivirus Packaging mix (Oxford Genetics) using lipo-fectamine following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Andrew R. McEwan, et al.,
bioRxiv - Genetics 2021
Quote:
... from human DNA (Cambio, UK) using the following primers ...
-
No products found
because this supplier's products are not listed.
Edward G. Meloni, et al.,
bioRxiv - Neuroscience 2023
Quote:
... with laboratory bedding (Alpha Chip; Northeastern Products Co.) and one square (5 x 5 cm) of nesting material (Nestlet; Ancare). All mice were maintained on 12/12 h light dark cycles (lights on at 700 h ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Parul Verma, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 100 µM L-alpha-phosphatidyl-D-myo-inositol 4,5-diphosphate, dioctanoyl (PIP2, KDR enhancer) were diluted in NbActiv4 recording media (BrainBits, Springfield, IL, USA). Complete saline solution (CSS ...
-
No products found
because this supplier's products are not listed.
Anna M.R. Hayes, et al.,
bioRxiv - Physiology 2023
Quote:
... Total starch in each diet was determined using a total starch analysis kit (amyloglucosidase/alpha-amylase method; Total Starch Assay Kit [AA/AMG], K-TSTA-50A, Megazyme, Wicklow, Ireland). Amounts of rapidly digestible starch (RDS) ...
-
No products found
because this supplier's products are not listed.
José Miguel Arcas, et al.,
bioRxiv - Neuroscience 2024
Quote:
Whole-cell voltage- and current-clamp recordings were obtained from mouse DRG neurons or transiently transfected HEK293 cells with borosilicate glass patch-pipettes (Sutter Instruments, 4-8 MΩ resistance) and were performed simultaneously with temperature recordings ...
-
No products found
because this supplier's products are not listed.
R. Oliver Goral, et al.,
bioRxiv - Neuroscience 2022
Quote:
... animals were single-housed in regular cages on white cellulose ALPHA-DRI bedding for 4-5 days and habituated to the rewarded sugar pellets (Dustless Precision Rodent Pellets, F05684, Bio-Serv, Flemington, NJ) with regular feed and water ad libitum ...
-
No products found
because this supplier's products are not listed.
Yun Jin Chae, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Tissue samples were incubated for 3 h at RT with the primary antibody (anti-CD8 alpha) before visualization with the Polink-2 HRP Plus Broad DAB Detection System for Immunohistochemistry kit (GBI Labs, Bothell, WA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
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No products found
because this supplier's products are not listed.
Christopher W. Benson, et al.,
bioRxiv - Genomics 2023
Quote:
Fungal DNA was isolated from tissue grown in culture on potato dextrose agar (PDA; Alpha Biosciences Inc., Baltimore, MD) using the Fungi/Yeast Genomic DNA Isolation Kit (Norgen Biotek Corp., Ontario, Canada). High molecular weight DNA was prepared for sequencing using the SMRTbell Template Preparation kit (v.1.0) ...
-
No products found
because this supplier's products are not listed.
Chanchal Thomas Mannully, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 1 μg/mL of human TGFβ1 (Biogems, Peprotech).
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No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Cunxi Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The Mpp75Aa1.1-His fractions identified by SDS-PAGE were pooled and further processed using EndoTrap® HD (Hyglos GmbH, Germany) to reduce endotoxin contents (manufacturer’s instruction) ...
-
No products found
because this supplier's products are not listed.
Yan Wang, Meiling Lian, Liping Song, Shengzhou Wu,
bioRxiv - Neuroscience 2020
Quote:
... fractions using commercially available kits (Cat#CSB-E08299h for human Aβ40; Cat#CSB-E10684h for human Aβ42, CUSABIO TECHNOLOGY LLC, Wuhan, China) per the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Takahiro Sanada, et al.,
bioRxiv - Microbiology 2022
Quote:
... AB human serum (Pel-Freez Biologicals, Rogers, AR, USA) was used as control serum.
-
No products found
because this supplier's products are not listed.
Alice Melocchi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... with 5% AB human serum (Access Biologicals, UC-CA) and 1% L-glutamine (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jeanne Corriveau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Human umbilical vein ECs (HUVECs) obtained from VEC Technologies were cultured in M199 media supplemented with 20% FBS ...
-
No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
-
No products found
because this supplier's products are not listed.
Sofia Rossini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... while recombinant human IDO1 protein was obtained by Giotto Biotech. Construct expressing murine Src was obtained from Origene.
-
No products found
because this supplier's products are not listed.
Padmapriya Sekar, et al.,
bioRxiv - Immunology 2023
Quote:
... 330 μg/mL iron-saturated human holo-transferrin (BBI solutions), 10 μg/mL recombinant human insulin (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Xiaowei Sun, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Recombinant human insulin (M9194) was purchased from AbMole (Houston, USA). The ARF1 inhibitor Golgicide A (HY-100540 ...
-
No products found
because this supplier's products are not listed.
Natsuki Shichinohe, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human bone ALP was purchased from CALZYME (San Luis Obispo, CA). Human CD73 His-tag was purchased from BPS Bioscience (San Diego ...
-
No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
-
No products found
because this supplier's products are not listed.
Erica Misner, Min Zhang, Eva Sapi,
bioRxiv - Microbiology 2022
Quote:
... PCR products were analyzed by standard agarose gel electrophoresis in a 1% gel referenced against a Hi-Lo DNA ladder (Bionexus, Inc., Oakland, CA, USA; BN2050). An infected zebrafish sample which was positive for B ...
-
No products found
because this supplier's products are not listed.
Camilla Kjeldgaard Larsen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The cleaved proteins were incubated with Ni-NTA beads for revers his-purification and cleaved proteins were collected in the flow through and visualized on SDS-PAGE (Mini Protean Tris-Tricine Precast gels, BioRad).
-
No products found
because this supplier's products are not listed.
Pirunthan Perampalam, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Cytokeratin (human specific cytokeratin 7 and 8, ZETA Corporation #Z2018ML, 1:200), EpCAM (Cell Signaling Technologies ...
-
No products found
because this supplier's products are not listed.
Erdem D. Tabdanov, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Human CD4+ T cells were plated on a glass-bottom dish (Willco Wells) pre-coated with either ICAM-1 (Life Technologies ...
-
No products found
because this supplier's products are not listed.
S. Karkampouna, et al.,
bioRxiv - Cell Biology 2021
Quote:
Mouse and human liver tissues were homogenised with UltraTurrax homogenizer (T25 basic, IKA) and TRIpure reagent (Roche) ...
-
No products found
because this supplier's products are not listed.
Guillaume N. Fiches, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 10% heat-inactivated human serum (Advanced Biotechnologies Inc, Cat#P2-203), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Brian D. Adair, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Chrono-luminescence reagent and human thrombin were purchased from Chrono-log (Havertown, PA).
-
No products found
because this supplier's products are not listed.
Barnabe D. Assogba, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Membranes were overnight probed in 1/1000 Rabbit Anti-Human APOBEC3G (Immunodiagnostics Inc.), washed three times ...
-
No products found
because this supplier's products are not listed.
Madelon M.E. de Jong, et al.,
bioRxiv - Immunology 2023
Quote:
... slides were incubated with anti-human CD15 (1:10, C3D-1, Diagnostic BioSystems) for 1 hour at 37°C ...
-
No products found
because this supplier's products are not listed.
Sonja Giger, et al.,
bioRxiv - Bioengineering 2021
Quote:
... recombinant human epidermal growth factor (hEGF, 20 ng/ml, Chimerigen Laboratories, CHI-HF-210EGF), recombinant human platelet derived growth factor (hPDGF-AB ...
-
No products found
because this supplier's products are not listed.
Rossana Aprigliano, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Patient-derived human induced pluripotent stem cells (hiPSCs) were generated by Applied StemCell (California, USA) by viral-free episomal reprogramming of JMS individual III-2 fibroblasts trough electroporation with three reprogramming plasmids containing human sequences for OCT4 ...
-
No products found
because this supplier's products are not listed.
Lisa Maria Metz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... For quantification of Panx1 plasma concentration in AAA patients a human Panx1 ELISA (#39097, Signalway Antibody) was performed ...
-
No products found
because this supplier's products are not listed.
Julia R. Port, et al.,
bioRxiv - Microbiology 2021
Quote:
... 200 µL blood was collected and were measured using the Piccolo® Lipid Panel Plus for humans (Abraxis) according to the manufacturer’s instruction.
-
No products found
because this supplier's products are not listed.
Chengyao Wang, et al.,
bioRxiv - Bioengineering 2020
Quote:
Human intestinal epithelial cells Caco-2 (ATCC® HTB-37™) were cultured in a 75 cm2 cell culture flask (Nest Biotechnology) with complete 1X Eagle’s Modified Eagle Medium (EMEM ...
-
No products found
because this supplier's products are not listed.
Hanyuan Shen, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
A431 cells were treated with different injections for 48 hours in 96-well plates and the cell culture supernatant was collected and tested for the level of IL-1β by ELISA using human interleukin-1 beta ELISA kit (Biosensis, CA, USA) according to the kit protocol ...
-
No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
... Human IgM anti-SARS-CoV-2 Spike (S1) Antibody CR3022 (Native Antigen, Oxford, UK) and anti-Spike RBD (SARS-CoV-2/COVID 19) human monoclonal antibody (eEnzyme, Gaithersburg, USA) were used as positive controls in ELISA ...
-
No products found
because this supplier's products are not listed.
Balázs Knakker, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... For stimulus presentation and data acquisition we used the CAmbridge Neuropsychological Test Automated Battery (CANTAB) specifically designed for non-human primates (Monkey CANTAB Cognitive Testing system with Intellistation and Whisker software, Campden Instruments, UK). The stimulus set (camcog_mdms0 ...
-
No products found
because this supplier's products are not listed.
Xiaoyi Zheng, et al.,
bioRxiv - Immunology 2021
Quote:
We quantified human serum Esm-1 by ELISA (Lunginnov, Lille, France) and mouse plasma Esm-1 by ELISA (Aviscera Biosciences, Santa Clara, CA), following the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Alaullah Sheikh, et al.,
bioRxiv - Microbiology 2022
Quote:
... in vitro grown differentiated polarized monolayers of human ileal enteroid samples as well as mouse intestinal biopsy samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Ted Pella, Inc., Redding, CA) in 100 mM sodium cacodylate buffer ...
-
No products found
because this supplier's products are not listed.
Xindi Li, et al.,
bioRxiv - Neuroscience 2024
Quote:
... human-derived microglia were subjected to a seven-day chronic intermittent exposure (CIE) paradigm using 200 Proof ethanol from Decon Laboratories (catalog# 2716) at concentrations of 0 mM (control) ...