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No products found
because this supplier's products are not listed.
Alexandria N. Hughes, Bruce Appel,
bioRxiv - Neuroscience 2019
Quote:
Bafilomycin A1 (Tocris, cat. 1334) was diluted to 1 mM in DMSO and stored at −20° ...
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Cat# HY-P70373-50 μg,
50 μg, USD $150.0
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Philip R Coryell, et al.,
bioRxiv - Cell Biology 2019
Quote:
... bafilomycin A1 (MedChemExpress HY-100558), or MG132 (MedChemExpress HY-13259).
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No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
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No products found
because this supplier's products are not listed.
Berislav Bošnjak, et al.,
bioRxiv - Immunology 2022
Quote:
... FCS files were analyzed using FCS Express V7 (De Novo Software) or FlowJo V10 (BD).
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No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
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No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
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No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
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No products found
because this supplier's products are not listed.
Jordana Griffiths, et al.,
bioRxiv - Immunology 2020
Quote:
... For domain binding determination PE-labelled secondary anti-mouse Fc antibody (Jackson Laboratories) was added ...
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No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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No products found
because this supplier's products are not listed.
Tugsan Tezil, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and Bafilomycin A1 (Adipogen) were dissolved in dimethyl sulfoxide (DMSO ...
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No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
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No products found
because this supplier's products are not listed.
Charlotte Degorre, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... then observed on laser confocal microscope (model FV1000, Olympus or Nikon-A1). Images were processed using Image J.
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No products found
because this supplier's products are not listed.
Cheng-Tsung Pan, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... A mouse 6X His tag antibody (HRP) (GeneTex Inc.) was then used to recognize Gal3-His at room temperature for 1 hour ...
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No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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No products found
because this supplier's products are not listed.
Arka Banerjee, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242) in the normal media described above ...
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No products found
because this supplier's products are not listed.
Ahmed A. Zarea, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... A1:100 dilution of anti-RFP antibody (Rockland Immunochemicals, 600-401-379) in 5% BSA / PBS was used for labeling (1 h) ...
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No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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No products found
because this supplier's products are not listed.
Simon J. Cleary, et al.,
bioRxiv - Immunology 2024
Quote:
... These sequences were codon-optimized and antibodies were expressed as chimeric hIgG1 with or without Fc point mutations in a HEK293 cell system and purified using protein A and buffer exchange (Absolute Antibody).
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No products found
because this supplier's products are not listed.
Yanrui Yang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... rabbit anti-endophilin A1 (Synaptic Systems GmbH, Germany); rabbit anti-Myc ...
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No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
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No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
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No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
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Bafilomycin A1(Baf-A1) is a vacuolar H+-ATPase inhibitor with IC50 of 0.44 nM. Bafilomycin A1 is...
Cat# S1413, SKU# S1413-1mg,
1mg, $347.00
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Nitin Sai Beesabathuni, Priya S. Shah,
bioRxiv - Systems Biology 2021
Quote:
Bafilomycin A1 (Selleck Chemicals, S1413), rapamycin (Selleck Chemicals ...
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No products found
because this supplier's products are not listed.
Danielle L. Michell, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... human Apolioprotein A1 ELISAPRO kits (Mabtech) were used to quantify apoA-I levels (as normalized to total protein ...
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No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
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No products found
because this supplier's products are not listed.
Rose Blake, et al.,
bioRxiv - Microbiology 2023
Quote:
... anti-His monoclonal antibody (Raybiotech) and goat anti-rabbit IgG were used as primary and secondary antibodies (Supplementary Table 1) ...
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No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
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No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
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No products found
because this supplier's products are not listed.
Mukundan Attur, et al.,
bioRxiv - Cell Biology 2019
Quote:
... supplemented with 10% FCS (Atlanta Biologicals). Cells at 50-70% confluency were transfected by overnight incubation with the indicated cDNAs in complete growth medium using TransIT®-LT1 - Transfection Reagent according to the manufacturer’s instructions (Mirus Bio ...
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No products found
because this supplier's products are not listed.
Sumona P. Dhara, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Reactions were quenched in heat inactivated fetal calf serum (HI-FCS; Gemini Bio-Products) in DMEM/F12 (Gibco ...
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No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
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No products found
because this supplier's products are not listed.
Adam T. Hilterbrand, et al.,
bioRxiv - Microbiology 2021
Quote:
... Bafilomycin A1 was purchased from ApexBio. Ammonium chloride was purchased from Fisher Scientific ...
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LC Laboratories' Product Number P-1680 - Phorbol 12-Myristate 13-Acetate (PMA, TPA,...
Cat# P-1680, SKU# P-1680_25mg,
25 mg, $339.00
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Nina Meyer, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and bafilomycin A1 (LC Laboratories, B-1018) for the indicated time periods ...
-
No products found
because this supplier's products are not listed.
Li Xu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
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No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
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No products found
because this supplier's products are not listed.
Amrita Sule, et al.,
bioRxiv - Cancer Biology 2021
Quote:
YFP-ATM was immunoprecipitated from stably transfected HEK293 cells by GFP-TRAP (Chromotek). The immunoprecipitates were suspended in kinase assay buffer containing 2 μCi of [γ-32P] ATP ...
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No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
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No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
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No products found
because this supplier's products are not listed.
Heng Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the immobilized FLAG-NBR1 was incubated with 1.5 μg His-MAPT and 1 μg GST or GST-BAG3 (Abnova H00009531-P01) in the BAG buffer (25 mM HEPES ...
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No products found
because this supplier's products are not listed.
Maitreyi Rathod, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse DSG1 ((#651111 Progen), rabbit keratin 10 (#905403 Biolegend) ...
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No products found
because this supplier's products are not listed.
Pablo Canales-Herrerias, et al.,
bioRxiv - Immunology 2023
Quote:
... Sections were stained with primary antibodies: Goat anti-mouse IgD (goat polyclonal GAM/IgD, FC/7S, Cedarlane Labs), biotin anti mouse GL7 (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
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Recombinant Human IL3RA (NP_002174.1) extracellular domain (Met 1-Arg 305), fused with the...
Cat# IL3RA-3248H,
20ug , USD $298
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
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No products found
because this supplier's products are not listed.
Peng Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Keigi Fujiwara (University of Texas MD Anderson Cancer Center, Houston, TX) and IP3R1-null HEK293 cells (Kerafast, Inc., Boston, MA) (Alzayady ...
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No products found
because this supplier's products are not listed.
Niko Schwenzer, et al.,
bioRxiv - Cell Biology 2024
Quote:
HEK293 cells with constitutive expression of CaV subunits β3 and α2δ1 and inducible expression of α1D (Charles River Laboratories CT6232) were cultured in DMEM/F12 medium containing selection antibiotics and 0.6 µM isradipine (Sigma I6658) ...
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No products found
because this supplier's products are not listed.
Mitsugu Shimobayashi, et al.,
bioRxiv - Physiology 2020
Quote:
Plasma Leptin levels were measured by mouse mouse Leptin ELISA kit (Crystal Chem) according to the manufacturer’s instructions.