-
No products found
because this supplier's products are not listed.
Pankaj Sharma, et al.,
bioRxiv - Immunology 2023
Quote:
... NP-OVA and NP-PE were procured from SouthernBiotech (Birmingham, AL, USA). B cell enrichment kit was purchased from Miltenyi Biotec (Bergisch Gladbach ...
-
No products found
because this supplier's products are not listed.
Juan García-Bernalt Diego, et al.,
bioRxiv - Microbiology 2023
Quote:
... Nucleoprotein or Hemagglutinin overlapping peptide pools (Miltenyi Biotec: PepTivator® SARS-CoV-2 Prot_S complete ...
-
No products found
because this supplier's products are not listed.
Fatima Amanat, et al.,
bioRxiv - Microbiology 2021
Quote:
... An anti-SARS-CoV nucleoprotein antibody (Novus Biologicals cat. NB100–56576) was used for IHC.
-
No products found
because this supplier's products are not listed.
Dibyadeep Datta, et al.,
bioRxiv - Neuroscience 2023
Quote:
... they were incubated in 70% Ethanol with 0.3% Sudan Black B (MP Biomedicals, Cat# 4197-25-5), to decrease autofluorescence from lipofuscin ...
-
No products found
because this supplier's products are not listed.
Marco Cassani, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Dox-NP (300112, Avanti Polar Lipids); tannic acid (403040 ...
-
No products found
because this supplier's products are not listed.
Balabhadrapatruni VSK Chakravarthi, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... NP-40 lysis buffer (Boston BioProducts, Ashland, MA) with protease inhibitor was used to lyse the cells ...
-
No products found
because this supplier's products are not listed.
Jian Hang Lam, et al.,
bioRxiv - Immunology 2022
Quote:
... with Trans IT-Virus GEN (Mirus). In brief ...
-
No products found
because this supplier's products are not listed.
Brian C. Ware, et al.,
bioRxiv - Immunology 2023
Quote:
... washed twice with PBS then resuspended in cold NP-40 lysis buffer (1% NP-40, 50mM Tris-Cl, 150mM NaCl) with protease inhibitors (Cell Signaling, #5871) on ice for 30 min ...
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No products found
Vivian Tam, et al.,
bioRxiv - Systems Biology 2020
Quote:
... AF and NP were then digested by 0.1% type II collagenase (Worthington Biochemical) for 14 hours and 0.05% type II collagenase for 8 hours ...
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No products found
because this supplier's products are not listed.
Robbert Boudewijns, et al.,
bioRxiv - Microbiology 2020
Quote:
... virus stocks were confirmed to be free of mycoplasma (PlasmoTest, InvivoGen) and other adventitious agents by deep sequencing on a MiSeq platform (Illumina ...
-
No products found
because this supplier's products are not listed.
Arthur Flohr Svendsen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Jenny Y. Pham, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ...
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Jiuzhi Xu, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Nucleoprotein was isolated from cells using a nucleoprotein extraction kit (AR0106, Bosterbio, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... sapiens-wt) glycoprotein from Advanced Biomart (San Gabriel, CA, USA) and the anti-Ebola surface glycoprotein [KZ52] from Absolute Antibody (Oxford, GB). The obtained EBOV-GP was expressed with a polyhistidine-tag at the C-terminus and consists of 629 amino acids ...
-
No products found
because this supplier's products are not listed.
Emily K Mantlo, et al.,
bioRxiv - Microbiology 2021
Quote:
Purified MACV (Carvallo strain) GP1 or NP (Immune Technology) were used as antigens ...
-
No products found
because this supplier's products are not listed.
Alena Salašová, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cells were lysed for 30min shaking in NP lysis buffer (3M Tris-HCl pH8 ...
-
No products found
because this supplier's products are not listed.
José-María Díez, et al.,
bioRxiv - Immunology 2020
Quote:
... Human Anti-MERS-NP IgG ELISA Kit (Alpha Diagnostic Intl. Inc.), against N protein ...
-
No products found
because this supplier's products are not listed.
Kelsey R. Nickerson, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... recombinant mNope-ECD-Fc protein (Biotechne/R&D Systems, 1294-NP) was added at 25 μg/ml (≍ 0.2 μM ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
David L. Goldblatt, et al.,
bioRxiv - Immunology 2019
Quote:
... Sections were stained with Sudan black B (Rowley Biochemical, Danvers, MA) for 1 h and counterstained with Kernechtrot’s nuclear fast red (Rowley ...
-
No products found
because this supplier's products are not listed.
Sidar Aydin, et al.,
bioRxiv - Immunology 2021
Quote:
... or the (VSV) nucleoprotein peptide (RGYVYQGL, BACHEM, Bubendorf, Switzerland) at 0.1 ng/mL for 30 minutes at 37°C or left unpulsed ...
-
No products found
because this supplier's products are not listed.
Sophie L. Winter, Petr Chlanda,
bioRxiv - Biophysics 2021
Quote:
... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Shenglan Shang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
-
No products found
because this supplier's products are not listed.
Santosh Phuyal, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 0.5% NP-40) and GFP- trap (ChromoTek, #gta-100) was performed following manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Joy S. Xiang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 0.66% NP-40) and sonicated with Bioruptor Pico (Diagenode) for 30 seconds on and 30 seconds off for a total of 5 minutes ...
-
No products found
because this supplier's products are not listed.
Luca Iamartino, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... or the negative CaSR modulator NPS 2143 (Tocris Bioscience) dissolved in dimethyl sulfoxide (DMSO) ...
-
No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Juan Jesus Vicente, et al.,
bioRxiv - Cell Biology 2024
Quote:
... then washed 2x with same buffer except NP-40 replaced by 1% PPS detergent (Expedeon). Immunoprecipitated complexes were released from beads with 0.2 M acetic acid and dried.
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
-
No products found
because this supplier's products are not listed.
Youssouf Sereme, et al.,
bioRxiv - Microbiology 2020
Quote:
... The virus-infected MRC5 cells were collected and centrifuged at 700 × g for 15 minutes (Eppendorf Centrifuge 5810R). Supernatant was filtered by serial filtration steps to eliminate cells and cellular debris ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
-
No products found
because this supplier's products are not listed.
Sandra M. Holmberg, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4-NP-N-acetyl-β-glucosamine and 4-NP-α-L-fucopyranoside (Carbosynth, UK). To calculate enzyme activity ...
-
No products found
because this supplier's products are not listed.
Emmanuelle Genoyer, et al.,
bioRxiv - Microbiology 2020
Quote:
Cells were lysed in NP-40 lysis buffer (Amresco) with proteinase inhibitors (Roche Boehringer Mannheim) ...
-
Recombinant Antigen
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Subhasis Mahari, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Human Immunodeficiency Virus (HIV) and Avian Influenza Virus (AIV) Ag were obtained from The Native Antigen Company (Oxford, UK). Aurdino software has been used in the in-house built device and the hardware include a printed Circuit Board (PCB) ...
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
-
No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
-
No products found
because this supplier's products are not listed.
M. Bormann, et al.,
bioRxiv - Microbiology 2021
Quote:
... the infectious virus was recovered by vortexing the carriers placed in plastic containers (SARSTEDT, Nümbrecht, Germany) filled with 2 mL DMEM for 1 minute ...
-
No products found
because this supplier's products are not listed.
Aline Alfaro, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The NPs were supported in a copper mesh covered with carbon (Support Films, Carbon Type-B, Ted Pella, inc.). The observations were performed with a TEM HT7700 (Hitashi ...
-
No products found
because this supplier's products are not listed.
Alex Agelidis, et al.,
bioRxiv - Cell Biology 2020
Quote:
... + 0.05% NP-40) and colorimetric substrate was detected at 410 nm using a microplate reader (Tecan GENious Pro, Mannedorf, Switzerland).