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No products found
because this supplier's products are not listed.
Ieva Bagdonaite, et al.,
bioRxiv - Microbiology 2022
Quote:
... plasmid encoding myc- and His-tagged full-length EBOV GP (Zaire ebolavirus isolate Ebola virus/H.sapiens-wt/SLE/2014/Makona-G3838) using Lipofectamine™ 3000 (Thermo Fisher Scientific Cat. Nr. L3000) according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Jérémy Dufloo, et al.,
bioRxiv - Microbiology 2024
Quote:
... The Ebola GP-encoding plasmid was obtained from Addgene. Information about RBPs is provided in Table S1.
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No products found
because this supplier's products are not listed.
Edward B. Irvine, et al.,
bioRxiv - Immunology 2021
Quote:
... Zaire ebolavirus glycoprotein (R&D Systems) was used as a negative control for the attenuated Mtb analysis.
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No products found
because this supplier's products are not listed.
Lorena Zuliani Alvarez, et al.,
bioRxiv - Immunology 2022
Quote:
... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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No products found
because this supplier's products are not listed.
Yuting Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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No products found
because this supplier's products are not listed.
Charlotte Guyomar, et al.,
bioRxiv - Cell Biology 2022
Quote:
... in the temperature (35 °C) and humidity-controlled atmosphere (∼95 %) of an automatic plunge-freezer (EM-GP, Leica). Then ...
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No products found
because this supplier's products are not listed.
Seyedehmahsa Moghimi, et al.,
bioRxiv - Microbiology 2022
Quote:
... expressing Moloney murine leukemia virus gag and pol genes was co-transfected with pLNCX2 vector with the FLAG-APEX2-GARG1060 insert and a plasmid coding for the vesicular stomatitis virus envelope glycoprotein (Takara Bio) using Mirus 2020 DNA transfection reagent (Mirus) ...
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No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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No products found
because this supplier's products are not listed.
Robert J. Ragotte, et al.,
bioRxiv - Biochemistry 2021
Quote:
... coli or Expi293™ cells underwent denaturation at 95 °C for 10 min in glycoprotein denaturing buffer (New England Biolabs) followed by immediate cooling on ice for 10 s ...
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No products found
because this supplier's products are not listed.
Zhongyan Lu, et al.,
bioRxiv - Immunology 2021
Quote:
... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
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No products found
because this supplier's products are not listed.
Dennis S. Metselaar, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... permeability glycoprotein (P-gp) (1:1000, #13978, Cell Signaling Technologies), CD31 (1:200 ...
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No products found
because this supplier's products are not listed.
Michael J. Ricciardi, et al.,
bioRxiv - Immunology 2022
Quote:
... To sequence the envelope from serum virus we isolated viral RNA (Qiagen), synthesized cDNA ...
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No products found
because this supplier's products are not listed.
Matthew H. Raymonda, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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No products found
because this supplier's products are not listed.
Masumi Namba, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Nephrin (GP-N2, Progen, Germany); Nestin (66259-1-Ig) ...
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No products found
because this supplier's products are not listed.
Sadra Bakhshandeh, et al.,
bioRxiv - Bioengineering 2023
Quote:
... sterile-filtered (0.22 μm; Steriflip-GP; Merck) and lyophilized ...
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No products found
because this supplier's products are not listed.
Alexandra Paul, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
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No products found
because this supplier's products are not listed.
Toufic Kassouf, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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No products found
because this supplier's products are not listed.
Wakako Furuyama, et al.,
bioRxiv - Microbiology 2021
Quote:
... or Lassa virus (LASV) GP peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APCCy7 ...
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No products found
because this supplier's products are not listed.
Yan Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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No products found
because this supplier's products are not listed.
Hiroyuki Mori, et al.,
bioRxiv - Microbiology 2022
Quote:
... Rabbit polyclonal anti-Ebola VP30 (GTX134035) was purchased from GeneTex (Irvine, CA). Rabbit polyclonal anti-CAPG antibody (10194-1-AP ...
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No products found
because this supplier's products are not listed.
MW Reginatto, et al.,
bioRxiv - Physiology 2021
Quote:
... solution to block nonspecific antibody binding sites and then incubated with primary antibodies against P-glycoprotein (P-gp-1:500; Santa Cruz Biotechnology, Texas, USA), breast cancer resistance protein (Bcrp-1:100 ...
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No products found
because this supplier's products are not listed.
Amy Flaxman, et al.,
bioRxiv - Immunology 2023
Quote:
... SUDV-Gulu GP (Sino Biologicals) and Bundibugyo (BDBV ...
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No products found
because this supplier's products are not listed.
Marissa D. Acciani, et al.,
bioRxiv - Microbiology 2020
Quote:
... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
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No products found
because this supplier's products are not listed.
Gretchen Harms Pritchard, et al.,
bioRxiv - Immunology 2019
Quote:
... 2016). LCMV Glycoprotein (GP) (provided by Dr. John Teijaro, Scripps Research Institute) was biotynylated and tetramerized with streptavidin-APC (Prozyme). Decoy reagent to exclude non-specific binding to the MSP1 tetramer was made by conjugating SA-PE to AF647 using an AF647 protein labeling kit (ThermoFisher) ...
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No products found
because this supplier's products are not listed.
Germano Costa-Neto, et al.,
bioRxiv - Genetics 2021
Quote:
... we refer to this model as “E-GP (BD)” ...
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No products found
because this supplier's products are not listed.
Katherine Minjee Chung, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and envelope (VSV-G) using TransIT-LT1 (Mirus Bio). Supernatant was collected at 48 and 72 hours and applied to target cells with 8 μg/mL polybrene (EMD Millipore ...
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No products found
because this supplier's products are not listed.
Heather Branscome, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and envelope was carried out with SYBR Green Master Mix (Bio-Rad) using the following primers ...
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No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
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No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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No products found
because this supplier's products are not listed.
Manuel Giménez-Andrés, et al.,
bioRxiv - Cell Biology 2020
Quote:
... an sCMOS PRIME 95 (Photometrics) camera ...
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No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
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No products found
because this supplier's products are not listed.
Fátima Lasala, et al.,
bioRxiv - Microbiology 2019
Quote:
... GP-A82 and single N-linked glycosylation mutant pseudoviruses in a 96-well white plate (Corning). After 48 h of incubation ...
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No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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No products found
because this supplier's products are not listed.
Vinayak Uppin, Shylaja M Dharmesh, R Sarada,
bioRxiv - Cancer Biology 2021
Quote:
... 95 % humidity (Eppendorf), and when the cells were confluent washed with PBS (pH 7.4 ...
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No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
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No products found
because this supplier's products are not listed.
Nobuyuki Oguri, et al.,
bioRxiv - Pathology 2023
Quote:
... anti-platelet glycoprotein (GP) IIb/IIIa (Sheep monoclonal, Affinity Biologicals Inc., Ancaster, Canada), glycophorin A (Dako/Agilent) ...
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No products found
because this supplier's products are not listed.
Lisa Dettinger, et al.,
bioRxiv - Microbiology 2021
Quote:
... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ...
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No products found
because this supplier's products are not listed.
Maj Schneider Thomsen, et al.,
bioRxiv - Neuroscience 2020
Quote:
To assess the functionality of the efflux transporter P-gp the Papp of the radiolabeled P-gp substrate [3H]-digoxin (PerkinElmer) was measured in the luminal to abluminal (L-A ...
-
No products found
because this supplier's products are not listed.
Jaclyn Beckinghausen, et al.,
bioRxiv - Neuroscience 2021
Quote:
... VGLUT1 (Ms and Gp, Synaptic Systems, 1:1,000); NFH (Ms ...
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No products found
because this supplier's products are not listed.
Yuting Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... MARV GP was purified using nickel column (GE Healthcare, 11003399). The concentration of proteins and antibodies were quantified by bicinchoninic acid (BCA ...
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No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... sapiens-wt) glycoprotein from Advanced Biomart (San Gabriel, CA, USA) and the anti-Ebola surface glycoprotein [KZ52] from Absolute Antibody (Oxford, GB). The obtained EBOV-GP was expressed with a polyhistidine-tag at the C-terminus and consists of 629 amino acids ...
-
No products found
because this supplier's products are not listed.
Pascal Brandys, et al.,
bioRxiv - Immunology 2022
Quote:
... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
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No products found
because this supplier's products are not listed.
Sophie Quick, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 95% and 100%] and acetone (EMS). Sections were then lifted into resin (Durcupan ACM ...
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No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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No products found
because this supplier's products are not listed.
Jiang-Hui Wang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
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No products found
because this supplier's products are not listed.
Maria Rojec, et al.,
bioRxiv - Systems Biology 2019
Quote:
... Cell envelopes were disrupted using a Bioruptor Plus sonication system (Diagenode s.a., Belgium) for 10 cycles ...