-
No products found
because this supplier's products are not listed.
Simon Hänle-Kreidler, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and cullin-RING E3 ubiquitin ligases with 5 µM MLN4924 (Cell Signaling Technology, 85923) for 5 h prior to cell harvest.
-
No products found
because this supplier's products are not listed.
Yijiun Zhang, Joachim Seemann,
bioRxiv - Cell Biology 2023
Quote:
... Expression of the E3 ubiquitin ligase component OsTIR1-2xMyc was induced by 0.5 μg/ml doxycycline (Sigma) for 6 h ...
-
No products found
because this supplier's products are not listed.
Malgorzata Piechota, et al.,
bioRxiv - Cell Biology 2022
Quote:
... E3 ligase reaction buffer (Boston Biochem), and ATP in 25 μl reaction mixture ...
-
No products found
because this supplier's products are not listed.
Bryan A. Killinger, et al.,
bioRxiv - Neuroscience 2023
Quote:
... ubiquitin antibody (Abcam) 1:10,000 diluted in blocking buffer overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Hannah Wapenaar, et al.,
bioRxiv - Biochemistry 2023
Quote:
... ubiquitin was detected using anti-ubiquitin antibody (Santa Cruz sc-8017) and HRP conjugated secondary antibody.
-
No products found
because this supplier's products are not listed.
Hyun-Soo Kim, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and 10x E3 Ligase Reaction Buffer (R&D systems B-71). Addition of Rik1-TAP complex purified from S ...
-
No products found
because this supplier's products are not listed.
Ajit Pal Singh, et al.,
bioRxiv - Plant Biology 2023
Quote:
... or anti-ubiquitin antibodies (Invitrogen #PA1-10023) and detected with HRP conjugated IgG (Invitrogen #31460 ...
-
No products found
because this supplier's products are not listed.
Orsolya Bilkei-Gorzo, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Total-ubiquitin antibody was purchased from DAKO (ZO458). Anti-Syntaxin 7 (sc-514017 ...
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No products found
because this supplier's products are not listed.
Alexander Hanzl, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... E3 ligase cDNAs were then cloned into pLenti6.2-ccdB-Nanoluc (Addgene 87075) via gateway LR-recombination cloning (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Yashwanth Ashok, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Proteins were detected with monoclonal antibodies against ubiquitin (BioLegend Cat. No. 646304; 1:1000), human HIF-1α (#610959 ...
-
No products found
because this supplier's products are not listed.
Muaz Nik Rushdi, et al.,
bioRxiv - Immunology 2022
Quote:
... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Suchitra Mohanty, et al.,
bioRxiv - Microbiology 2020
Quote:
... Ubiquitin antibody was purchased from Enzo Life Sciences. DAPI (4’ ...
-
No products found
because this supplier's products are not listed.
Teresa Zulueta-Coarasa, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Embryos used for imaging were transferred to E3 with 0.003% N-phenylthiourea (PTU-E3, Merck) at 24 hpf to inhibit pigmentation ...
-
No products found
because this supplier's products are not listed.
Alejandro R. Gener, Jason T. Kimata,
bioRxiv - Genomics 2019
Quote:
... The ligase used: T4 DNA ligase (NEB). Libraries were loaded onto one of 5 MinION RevD flow cells ...
-
No products found
because this supplier's products are not listed.
Christos Andreadis, Tianhao Li, Ji-Long Liu,
bioRxiv - Cell Biology 2021
Quote:
... the ubiquitin rabbit polyclonal antibody was used (10201-2-AP, Proteintech). Tubulin or actin were used as loading controls and the detection was done by using anti-tubulin antibody (Sigma T5168 ...
-
No products found
because this supplier's products are not listed.
Shotaro Namba, Hisao Moriya,
bioRxiv - Cell Biology 2024
Quote:
... and ubiquitin was blocked with anti-ubiquitin was detected using an anti-GFP antibody (Roche) and ubiquitin was detected using an anti-ubiquitin antibody (Santa Cruz) ...
-
No products found
because this supplier's products are not listed.
Yashwanth Ashok, et al.,
bioRxiv - Biochemistry 2021
Quote:
... WT ubiquitin was produced in-house while single lysine ubiquitin and K0 ubiquitin were obtained from LifeSensors (Cat. # SI210).
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No products found
because this supplier's products are not listed.
Johannes Popow, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Target engagement and intracellular availability for VHL ligands were assessed using the NanoBRET TE Intracellular E3 ligase assay (Promega). HEK293 cells were transfected with the VHL-NanoLuc fusion constructs using FuGENE HD (Promega ...
-
No products found
because this supplier's products are not listed.
Qianyan Li, et al.,
bioRxiv - Genetics 2022
Quote:
... Worm lysates or E3 ligase reaction mixtures were resolved on 4-20% stain-free SDS-PAGE gels (Bio-RAD) and transferred to Millipore Immobilon-P PVDF membranes ...
-
No products found
because this supplier's products are not listed.
Fugen Yu, et al.,
bioRxiv - Plant Biology 2024
Quote:
The yeast two-hybrid assays were carried out using a mating-based split ubiquitin system (mbSUS) for membrane protein interactions as described “mbSUS tests protocol” (Clontech) (Obrdlik et al. ...
-
No products found
because this supplier's products are not listed.
Wenxin Zhang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... E3 containing fractions were pooled and loaded onto a ResQ anion exchange chromatography column (GE Healthcare). The E3 complex was eluted by applying a salt gradient from 50 to 700 mM NaCl (ResQ buffer base ...
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No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... anti-ubiquitin (Agrisera), anti-GFP (SantaCruz) ...
-
No products found
because this supplier's products are not listed.
Jessica L. Rausch, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and mono-ubiquitin (BD Pharmingen).
-
No products found
because this supplier's products are not listed.
Fia B. Larsen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... pcDNA3.1 expressing strep-Myc-tagged ubiquitin (Genscript) was used.
-
No products found
because this supplier's products are not listed.
Jessica J. Miciak, Fred Bunz,
bioRxiv - Cancer Biology 2023
Quote:
... The isolation of ubiquitinated proteins was performed with the Signal-Seeker Ubiquitin Enrichment kit (Cytoskeleton). Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
-
No products found
because this supplier's products are not listed.
Hirotatsu Imai, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Antibodies against ribosomal proteins uL3 (GTX114725, GeneTex), L10a (A305-061A ...
-
No products found
because this supplier's products are not listed.
Jayashree Kadirvelu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... anti-ubiquitin (Novus Biologicals, NBP2-30132), c-myc (SC Biotech ...
-
No products found
because this supplier's products are not listed.
Fanny Eyboulet, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Kathrin Leppek, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Ligase I (Lucigen) at 68°C for 2 h ...
-
No products found
because this supplier's products are not listed.
Nilsa La Cunza, et al.,
bioRxiv - Neuroscience 2020
Quote:
... E3 or E4 (Amaxa Nucleofector II, Lonza, Rockland, ME). Approximately 1.5 million cells and 5 μg plasmid DNA were used for each transfection ...
-
No products found
because this supplier's products are not listed.
Gianluca Mucciolo, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Proteins were detected using HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories).
-
No products found
because this supplier's products are not listed.
Audrey Noireterre, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... or 1 μL of anti-Ubiquitin antibodies (Calbiochem ST1200-100UG clone FK2), or 20 μL of Dynabeads Pan Mouse IgG (Invitrogen 11041 ...
-
No products found
because this supplier's products are not listed.
Jeffery M.R.B. McAlpine, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Labelled ubiquitin was visualised using an Odyssey Fc system (LI-COR) at 600 nm with a 2 min exposure ...
-
No products found
because this supplier's products are not listed.
Trey Farmer, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Antibodies against the following proteins were used: RPL3 (Bethyl Laboratories #A305-007A,1:100 for immunofluorescence) ...
-
No products found
because this supplier's products are not listed.
Conrad En-Zuo Chan, et al.,
bioRxiv - Microbiology 2022
Quote:
The rabbit polyclonal antibody to S protein (polyS) (Sino Biological, Singapore) and anti-N (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Yuxin Wang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and E3 ligase recruiter building blocks thalidomide - linker 2 (#6468/25) were purchased from Tocris Bioscience (Minneapolis ...
-
No products found
because this supplier's products are not listed.
François-Xavier Lehr, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... with the help of an automatic dispenser (Eppendorf Repeater E3). The volume was then brought up with plasmid DNA constructs containing the genetic circuits and nuclease-free water (0.5 ...
-
No products found
because this supplier's products are not listed.
Lorena Sanchez Felipe, et al.,
bioRxiv - Microbiology 2020
Quote:
... and HRP conjugated secondary antibody (Protein Simple). Chemiluminescence signals were analyzed using Compass software (Protein Simple) ...
-
No products found
because this supplier's products are not listed.
Akshaya Ashok, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Rabbit anti-Ubiquitin (Cell signalling Technology-#3933S) and Rabbit anti-Yap (Abclonal-#A1002)] ...
-
No products found
because this supplier's products are not listed.
Patrik Risteski, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and human anti-centromere protein antibody (Antibodies Incorporated). The secondary antibodies used were donkey anti-mouse IgG-Alexa Fluor 488 (Abcam) ...
-
No products found
because this supplier's products are not listed.
Dorota Raj, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
-
No products found
because this supplier's products are not listed.
Zachary S. Wilson, et al.,
bioRxiv - Immunology 2019
Quote:
... and then reconstituted by intravenous injection of bone marrow cells from a Vclf/fMX1creGFP+ mouse expressing transgenic enhanced green fluorescent protein (EGFP) under the ubiquitin C promoter (The Jackson Laboratory) and a Vclf/f (GFP- ...
-
No products found
because this supplier's products are not listed.
Kit-Yi Leung, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... GLDC protein abundance was determined using anti-GLDC antibody (Atlas antibodies), with GAPDH (detected using anti-GAPDH ...
-
No products found
because this supplier's products are not listed.
Jamie Guenthoer, et al.,
bioRxiv - Immunology 2023
Quote:
... A SARS-CoV-2 nucleocapsid protein monoclonal antibody (Bioss Antibodies, clone 1C7) solution was prepared at 1 μg/mL and added to all wells for one hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Jincong Zhou, et al.,
bioRxiv - Plant Biology 2019
Quote:
... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
-
No products found
because this supplier's products are not listed.
Vlada V. Zakharova, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
-
No products found
because this supplier's products are not listed.
Tom Rankenberg, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Protein-DNA complexes were immunoprecipitated using anti-HA antibodies (Miltenyi Biotec) (Kaufmann et al. ...
-
No products found
because this supplier's products are not listed.
Silvio Schmidt, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Primary antibodies against the activity-regulated cytoskeleton-associated protein (ARC; guinea pig, Synaptic system), the glial fibrillary acidic protein (GFAP ...
-
No products found
because this supplier's products are not listed.
Emily K Don, et al.,
bioRxiv - Neuroscience 2020
Quote:
... flat bottom 96-well plate (Corning; 1 fish per well in 200 ml E3). Prior to experiments ...
-
No products found
because this supplier's products are not listed.
Federica Diofano, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... E3 medium was maintained at 28 °C in a 14 cm diameter Petri dish (VWR), into which 72 hpf embryos were placed in the centre of the dish individually ...