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No products found
because this supplier's products are not listed.
Sean J. Ravel, Victoria M. Hollifield,
bioRxiv - Microbiology 2023
Quote:
... Controls samples included an 8-member positive controls (Zymo Research), as well as an extraction negative and a PCR negative controls ...
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Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Cat# LS001643,
5x1 mg, $166.00
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M Fejzo, et al.,
bioRxiv - Genetics 2023
Quote:
... To digest the polypeptide 10 µL of Glu C enzyme (Worthington) at 100 µg/mL was added and the samples digested overnight at 37 °C ...
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No products found
because this supplier's products are not listed.
Emily S. Mathews, et al.,
bioRxiv - Microbiology 2019
Quote:
... Primary antibodies used include anti-HSP40 (1:250) and anti-PDI (1D3) mouse 1:100 (ADI-SPA-891-D; Enzo Life Sciences, Farmingdale, NY). Secondary antibodies were added at 1:30 for 1 hour at room temperature ...
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No products found
because this supplier's products are not listed.
Alexandre M. Paré, et al.,
bioRxiv - Cell Biology 2024
Quote:
The DNAJ cDNA (corresponding to residues 4316-4420 of sacsin) was subcloned using mouse pEGFP-sacsin full length cDNA (OriGene Technologies, Rockville, MD, USA) and inserted in frame with GST into the pGEX6 vector ...
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No products found
because this supplier's products are not listed.
Helena Hinterding, et al.,
bioRxiv - Genetics 2023
Quote:
... The IBD between long-lived family members was calculated using the --ibd module from Merlin using previously generated genome-wide genetic data from Illumina BeadChips 72.
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No products found
because this supplier's products are not listed.
Yutaro Hama, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and guinea pig polyclonal antibodies against p62 (GP62-C; PROGEN) as primary antibodies ...
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No products found
because this supplier's products are not listed.
Alexandra Schäfer, et al.,
bioRxiv - Microbiology 2021
Quote:
Even groups (n=10, or less as indicated) of 10-week-old female BALB/c mice (Envigo; #047) were used in all in vivo efficacy studies ...
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No products found
because this supplier's products are not listed.
Zhenzhen Wang, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... or 2) subsequently stimulated with 5 μg/kg T cell chemokine – C-X-C motif chemokine ligand 10 (CXCL10, PeproTech, Rocky Hill, USA) (23) ...
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No products found
because this supplier's products are not listed.
Mehran Najibi, et al.,
bioRxiv - Immunology 2019
Quote:
... Anti-Mucolipin 1 (MCOLN1) (C-Term) antibody (Antibodies-Online, ABIN571446). Cells were washed thrice in PBS and incubated with the fluorescent secondary antibody plus Hoechst stain (Anaspec ...
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No products found
because this supplier's products are not listed.
Tsan-Ting Hsu, Chien-Yao Wang, Yi-Ping Hsueh,
bioRxiv - Neuroscience 2023
Quote:
... with intensified oChIEF-Citrine (stained with GFP antibody) and C-FOS (stained with C-FOS antibody) signals were imaged with a high-content imaging system (Molecular Devices) hosting a 4x objective ...
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No products found
because this supplier's products are not listed.
Marine Oberkampf, et al.,
bioRxiv - Microbiology 2021
Quote:
... The remainder of the sample was heated to 95°C for 10 min and used to quantify c-di-AMP using a c-di-AMP ELISA kit (Cayman Chemical) according to the recommendations of the supplier ...
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No products found
because this supplier's products are not listed.
Duo Xu, et al.,
bioRxiv - Immunology 2023
Quote:
... we immunized two groups of BALB/c mice (n=10) with 5 µg protein antigens adjuvanted with 10 µg Monophosphoryl lipid A (MPLA, Invivogen) and 10 µg Quil-A (Invivogen ...
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No products found
because this supplier's products are not listed.
Clement Gallay, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cellular growth was then monitored every 10 min at either 37°C or 30°C in a microtiter plate reader (TECAN Infinite F200 Pro). Each growth assay was performed in triplicate ...
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No products found
because this supplier's products are not listed.
Christopher I Cazzonelli, et al.,
bioRxiv - Plant Biology 2019
Quote:
... 10 μg of total protein was loaded to the gel and anti-DET1 polyclonal antibody (Agrisera Antibodies AS153082) was used at a 1:1000 dilution ...
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No products found
because this supplier's products are not listed.
Jonathan M. Goodwin, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were fixed for 10 min in either -20°C methanol or 4% paraformaldehyde (Electron Microscopy Sciences). Cells were blocked and permeabilized in a solution containing 1:1 Odyssey blocking buffer (LiCor)/PBS (Invitrogen ...
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No products found
because this supplier's products are not listed.
Emily E. Blythe, Mark von Zastrow,
bioRxiv - Cell Biology 2023
Quote:
... 5 mM d-glucose) twice before a 10 minute incubation in a 37°C plate reader (Synergy H4, BioTek), optionally with DMSO or Dyngo4a (30 μM ...
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No products found
because this supplier's products are not listed.
Aisha A. AlJanahi, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... CD34+ cells were purified from the apheresis PBMC collection via immunoabsorption and cultured overnight in at 37°C in X-VIVOTM 10 (Lonza) supplemented with 1% HSA (Baxter ...
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No products found
because this supplier's products are not listed.
Sonny R. Elizaldi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All 4 µm paraffin sections were subjected to a heat antigen retrieval step before application of primary antibodies by treating slides with AR-10 (Biogenex) for 2 minutes at 125°C in a Digital Decloaking Chamber (Biocare), followed by cooling to 90°C for 10 minutes ...
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No products found
because this supplier's products are not listed.
Gitanjali A. Narayanan, et al.,
bioRxiv - Immunology 2019
Quote:
... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...
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No products found
because this supplier's products are not listed.
Luka Jarc, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... C-peptide detection was performed using the human C-peptide ELISA kit (Mercodia, 10-1141-01), readings were taken using an ELISA plate reader and the standard curve was generated ...
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No products found
because this supplier's products are not listed.
Jennifer Schwarz, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 1×107 cells were starved for 30 min at 37°C in 1 ml FCS-free RPMI and then treated with 10 µg/ml anti-λ antibody (Southern Biotech) under shaking at 750 rpm for the indicated time points ...
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No products found
because this supplier's products are not listed.
Thomas Fischer, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... at 37°C in 5% CO2 on 10 cm dishes (Greiner Bioscience). For cell detachment Trypsin (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Marina Vortmeyer-Krause, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20×, NA 0.30, Leica; and 10×, NA 0.25, Nikon DiaPhot 300). Images were collected by CCD cameras (Sentech or Hamamatsu C8484-05G) ...
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No products found
because this supplier's products are not listed.
Linh Le, et al.,
bioRxiv - Cell Biology 2020
Quote:
... All cells were cultured at 37°C and 10% CO2 in RPMI 1640 medium supplemented with 10% FBS (Atlanta Biologicals) and 200 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) ...
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No products found
because this supplier's products are not listed.
Shuang Wang, et al.,
bioRxiv - Microbiology 2020
Quote:
... Then 10 μL anti-FcγRI antibody-FITC (Cat: 10256-R401-F, Sino Biological), anti-FcγRIIa antibody-FITC (Cat ...
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No products found
because this supplier's products are not listed.
Yuanjiu Lei, et al.,
bioRxiv - Immunology 2023
Quote:
Circular Z-and B-DNA were diluted to 10 ng/µL (0.43 µM) in 10 mM HEPES buffer (10 mM MgCl2, pH 7.5) and incubated with anti-Z-DNA antibody (Absolute antibody, Ab00783-23.0) at a 1:1 or 1:3 DNA:antibody molar ratio for 4 h at 25 °C ...
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No products found
because this supplier's products are not listed.
Yung-Tian A. Gau, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000 ...
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No products found
because this supplier's products are not listed.
Shunji Nakamura, Tokitaka Oyama,
bioRxiv - Plant Biology 2021
Quote:
... http://www.hamamatsu.com/) under a macro zoom microscope (MVX-10 with an MVPLAPO 2×C lens; Olympus Optical ...
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No products found
because this supplier's products are not listed.
Fatima Amanat, et al.,
bioRxiv - Microbiology 2022
Quote:
... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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No products found
because this supplier's products are not listed.
Adeline Simon, et al.,
bioRxiv - Genomics 2022
Quote:
... Presence of the subfamilies in the genomes of additional strains (Table 1) was tested by PCR using the MyTaq polymerase (Bioline) and the primers listed in Sup ...
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10-Gingerol is a bioactive compound found in ginger with anti-inflammatory and antioxidant activity.
Cat# S9091, SKU# S9091-1mg,
1mg, $147.00
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Giovanni C. Forcina, et al.,
bioRxiv - Cell Biology 2021
Quote:
A 261-member bioactive compound library were obtained from Selleck Chemicals (Cat# L2000), formatted as described previously (6) ...
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No products found
because this supplier's products are not listed.
Maik Wolfram-Schauerte, et al.,
bioRxiv - Microbiology 2024
Quote:
... -10 °C for 10 min and aqueous upper phase was filtered through 0.2 µm PFTE filters (Phenomenex) and stored at -80 °C until analysis.
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No products found
because this supplier's products are not listed.
Chengyuan Wang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... concentrated by centrifugation at 4,000xg at 4°C through VivaSpin 10 kDa MWCO concentrators (Sartorius), aliquotted ...
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No products found
because this supplier's products are not listed.
Pelin Kaya, et al.,
bioRxiv - Biochemistry 2023
Quote:
... after 10 μM coelenterazine 400a (#C-320, Gold Biotechnology) addition ...
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No products found
because this supplier's products are not listed.
Dmitry Sutormin, et al.,
bioRxiv - Molecular Biology 2021
Quote:
10 µg of S9.6 antibodies (Kerafast, ENH001) were incubated with 20 µL of protein-G Sepharose (Bialexa ...
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Cat# HY-126297-25 mg,
25 mg, USD $1100.0
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Ling-Zhen Liu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were treated with 0–10 μM compound C or 0–20 μM SBI-0206965 (MedChemExpress, Cat HY-13418 and Cat HY-16966 ...
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No products found
because this supplier's products are not listed.
Mireille M.J.P.E. Sthijns, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The absorbance was measured for 10 minutes at 37°C on a microplate reader (CLARIOstar, BMG LABTECH) at 412 nm and the slope of the curve was calculated ...
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No products found
because this supplier's products are not listed.
Eric W. Fowler, et al.,
bioRxiv - Bioengineering 2021
Quote:
Expression of TGF-β family members was investigated using a Human TGF beta Array C2 (AAH-TGFB-2-2; RayBiotech®, Peachtree Corners, GA). Medium collected from hydrogel cell constructs from days 3-6 was pooled from three biological replicates and used to carry out the assay following the manufacturer’s procedure ...
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No products found
because this supplier's products are not listed.
Richard Drexler, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and incubated in secondary antibodies (1:10 10 nm gold-conjugated IgG, TED Pella, 15796) for 1 h at room temperature and rinsed with PBST followed by water ...
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No products found
because this supplier's products are not listed.
Alice Abreu Torres, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 10% heat-treated (56 °C, 1 h) foetal bovine serum (FBS, Pan Biotech), 100 U/mL penicillin and 100 μg/mL streptomycin (P/S ...
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No products found
because this supplier's products are not listed.
Dayana C. Farhat, et al.,
bioRxiv - Microbiology 2021
Quote:
... cultures were ice cooled to 20°C for 10 min then induced with 500 μM of IPTG (Euromedex) for 12h after which cultures were centrifuged and stored as dry pellets at −80°C.
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No products found
because this supplier's products are not listed.
Audrey Tze Ting Khoo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... (ii) the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA ...
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No products found
because this supplier's products are not listed.
Yan Zhang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... was typically acquired at eight to ten frames using a 10× C Epiplan-Apochromat objective (0.4-NA, 5.4-mm free working distance, Carl Zeiss) at typically 512×512 pixel resolution with lasers tuned at 488 nm and at 561 nm and emission at 500–550 nm for green and 620–700 nm for red fluorescence ...
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No products found
because this supplier's products are not listed.
Alexandre D’Halluin, et al.,
bioRxiv - Microbiology 2022
Quote:
... followed by centrifugation at 5000 rpm for 10 minutes at 4°C and finally RNA extraction using the FastRNA Pro Blue Kit (MP Biomedicals) following the instruction of the supplier ...
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No products found
because this supplier's products are not listed.
Rupert L. Mayer, et al.,
bioRxiv - Immunology 2022
Quote:
... The Ultimate 3000’s column oven was set to 50°C and for proper ionization a fused silica PicoTip emitter (10 μm inner diameter, New Objective, Littleton, MA, US) was connected to the μPAC outlet union and a grounded connection was provided to this union ...
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No products found
because this supplier's products are not listed.
Benjamin J. Reisman, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The crude F1 preparation was loaded onto a 5mL HisTrap HP 5 mL column at 4 °C and washed with 300 mL of 97 % wash buffer (10% MeOH, 10% glycerol, 0.3M NaCl ...
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No products found
because this supplier's products are not listed.
Michaela S. Matthes, et al.,
bioRxiv - Plant Biology 2024
Quote:
... 37°C) and secondary rabbit Cy3-Antibodies (BSA/PBS for 3.5h at 37°C; Jackson ImmunoResearch/USA supplied by Dianova/Hamburg). After repeated washes with PBS and H20 the embryos were embedded in Citifluor antifadent mounting medium and covered with a coverslip ...
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No products found
because this supplier's products are not listed.
Eric Cortada, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Sections were incubated overnight at 4 °C with anti-Mac2 antibody (Cedarlane cat. CL8942AP), followed by incubation with corresponding biotinylated secondary antibodies ...
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No products found
because this supplier's products are not listed.
Juan Martín D’Ambrosio, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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No products found
because this supplier's products are not listed.
Lyan van der Sleen, et al.,
bioRxiv - Biophysics 2023
Quote:
... a solution of 500 μM glycine betaine in buffer C was added in steps of 2 or 10 μL using a Hamilton syringe pump (Harvard apparatus). Excitation and emission wavelengths of 295 and 360 nm (and slit widths of 1 and 5 nm) ...