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No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
N-terminally His-tagged Pasteurella multocida toxin (PMT) was coated at 10 µg/mL (500 ng/well ...
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No products found
because this supplier's products are not listed.
Joshua T. Rose, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... cholera toxin to 100ng/mL (Thomas Scientific, Swedesboro, NJ), hydrocortisone to 500ng/mL (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Sweta Ghosh, et al.,
bioRxiv - Microbiology 2023
Quote:
... Difficile Toxin AB Qualitative ELISA Assay Kit (Eagle Biosciences). Cells were removed from the supernatant via centrifugation ...
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No products found
because this supplier's products are not listed.
Michelle Wang, Sheya Xiao Ma, Andrew J. Darwin,
bioRxiv - Microbiology 2023
Quote:
... coli Δcya mutant strain BTH101 (Euromedex) by calcium chloride transformation ...
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No products found
because this supplier's products are not listed.
Feng Long, et al.,
bioRxiv - Genomics 2020
Quote:
... Wild-type or mutant SMOC2 EC domain (Bioss) was added to the wells at increasing doses (10 ...
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No products found
because this supplier's products are not listed.
Chad W. Hicks, et al.,
bioRxiv - Biophysics 2024
Quote:
... acidic patch mutant H2A(E61A) (EpiCypher 16-0029); H2AK119ub1 (EpiCypher 16-0395) ...
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No products found
because this supplier's products are not listed.
Joshua R. Fletcher, et al.,
bioRxiv - Microbiology 2022
Quote:
... JE2 and fadX::tn transposon mutant (obtained from the Nebraska Transposon Mutant Library) were routinely cultured aerobically at 37ºC on LB agar (IBI Scientific IB49020) or with shaking at 220 rpm in LB broth ...
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No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
Protein toxins were incubated with either human sera (Human Serum age 4-6, Innovative Research) or PBS in a 1:1 ratio ...
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No products found
because this supplier's products are not listed.
Julia L. E. Willett, et al.,
bioRxiv - Microbiology 2023
Quote:
... Tn mutants were inoculated using a metal replicating pin (Boekel Scientific) into 2 mL deep 96-well plates (Biotix ...
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No products found
because this supplier's products are not listed.
Vera Kovaleva, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The MANF K96A and K128A C130S mutant recombinant proteins were produced by Icosagen using the same technology ...
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No products found
because this supplier's products are not listed.
Andrés M. Baraibar, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Toxins ω-conotoxin-GVIA (ω-CTx-GVIA) and ω-agatoxin-IVA (ω-Aga-IVA) (Peptide Institute, Osaka, Japan) were dissolved in distilled water at 0.1 mM stock concentration and stored frozen.
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No products found
because this supplier's products are not listed.
Marta Rivas, et al.,
bioRxiv - Microbiology 2021
Quote:
All fecal samples were screened with the SHIGA TOXIN QUIK CHEK (STQC) test (Abbott™, TechLab®, Blacksburg, VA) according to the manufacturer’s instructions and the standard guidelines established by the National Reference Laboratory of Argentina ...
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No products found
because this supplier's products are not listed.
Stephen F. Fitzgerald, et al.,
bioRxiv - Microbiology 2020
Quote:
... The level of Stx toxin in each sample was assayed using the RIDASCREEN® Verotoxin ELISA kit (R-Biopharm) according to manufacturer guidelines ...
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No products found
because this supplier's products are not listed.
Benjamin B. Kim, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Mutants were expressed and screened in constitutively active bacterial expression vector pNCS (Allele Biotech). Plasmids were transformed into chemically competent XL-10 Gold (Agilent) ...
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No products found
because this supplier's products are not listed.
Poornima Bhat-Nakshatri, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... PIK3CA antibody that preferentially recognizes H1047R mutant was purchased from Assay Biotechnology (Catalogue # V0111). Phospho-p65 antibody (Ser 536 ...
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No products found
because this supplier's products are not listed.
J. Matthew Watson, et al.,
bioRxiv - Genetics 2019
Quote:
... All mutants and genotyping conditions have been previously described: tert (Fitzgerald, Riha et al., 1999), ku70 (Riha et al. ...
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No products found
because this supplier's products are not listed.
Eleni Petsouki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Nde1-null C3H10T1/2 or double mutant cell lysates were incubated with α-FBW7 antibody (Abnova) overnight to immunoprecipitate FBW7 ...
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No products found
because this supplier's products are not listed.
Jack A. Bryant, et al.,
bioRxiv - Microbiology 2023
Quote:
... Δskp and ΔdegP mutants were transformed with the EZ-Tn5™ Tnp Transposome (Cambio) as previously described (Goodall et al. ...
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No products found
because this supplier's products are not listed.
Li He, Jiayu Wen, Qi Dai,
bioRxiv - Developmental Biology 2023
Quote:
... Prdm16_E and Prdm16 mutant NSCs were cultured in NeuroCult™ Proliferation Kit (Stem cell technologies, 05702) for 3 days with or without BMP4 at concentration of 25ng/ml medium (Sigma ...
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No products found
Asiya Gusa, et al.,
bioRxiv - Microbiology 2020
Quote:
... Rapamycin/FK506-resistant mutants were selected on YPD supplemented with 1 μg/mL of rapamycin (LC Laboratories) and 1 μg/mL FK506 (Astellas) ...
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No products found
because this supplier's products are not listed.
Kaori Matsuyama, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... WT and mutants were purified by using SkillPak TOYOPEARL Phenyl-650M (c.v. = 5 ml, Tosoh, Tokyo, Japan) equilibrated with 20 mM sodium acetate buffer ...
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No products found
because this supplier's products are not listed.
Wolf-Rűdiger Scheible, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Arabidopsis WT and phr1 mutants were germinated on ½ MS media with vitamins (M519, PhytoTechnology Laboratories, KS, USA) buffered to pH 5.7 with 150 mg/L MES ...
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No products found
because this supplier's products are not listed.
Kathrin Olschok, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... or goat anti-mouse IgG Alexa Fluor 647 (for CALR mutant, clone poly4053, BioLegend, San Diego, CA, USA) for 1 h in the dark ...
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No products found
because this supplier's products are not listed.
Johan Zeelen, et al.,
bioRxiv - Microbiology 2020
Quote:
... and VSGsur-H122A mutants were cultivated in vitro in HMI-9 media (formulated as described by PAN Biotech without FBS ...
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No products found
because this supplier's products are not listed.
Thomas G. Flower, et al.,
bioRxiv - Biophysics 2020
Quote:
... Selenomethionine labeled ORF8 double mutant protein was expressed using SelenoMet Medium Base according to manufacturer’s instructions (Molecular dimensions). Purification was carried out in the same manner as wild-type but the TEV-cleavage step was omitted.
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No products found
because this supplier's products are not listed.
Hiep Tran, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and Fmr1 mutants was reserve transcribed to cDNA using MMLV High-Performance Reverse Transcriptase Kit (Biosearch Technologies #RT80125K) and oligo(dT ...
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No products found
because this supplier's products are not listed.
Dorothee Jakob, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... we applied the spider toxin peptide Grammostola spatulata mechanotoxin 4 (GsMTx4) L-isomer (10 μmol/L, H20 as solvent, CSBio, Menlo Park, CA, USA), a known blocker of cation non-selective SAC ...
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No products found
because this supplier's products are not listed.
Can Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The mutants were initially screened in pea broth medium amended with 30 μg/ml geneticin (Amresco, Solon, United States) and then on V8 agar plates containing 50 μg/ml geneticin ...
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April L. Solon, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells expressing mCherry and mCherry-KIFBP (wild type or indicated mutants) were treated with 20 μM MG132 (Selleck Chemicals) for 2 hours before fixation ...
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No products found
because this supplier's products are not listed.
Sauratej Sengupta, et al.,
bioRxiv - Bioengineering 2022
Quote:
... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
-
No products found
because this supplier's products are not listed.
Norman Tran, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 5 mg/mL H163A mutant (∼148 µM) was incubated with 400 µM GC376 (BPS Bioscience; San Diego, CA, USA) at room temperature for two hours prior to setting up the crystallization experiment ...
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No products found
because this supplier's products are not listed.
Jun Cao, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mutant sequence or wild-type sequence of HDAC6 were respectively cloned on the overexpression plasmid vector Lv206 (GeneCopoeia) (Fig EV5B) ...
-
No products found
because this supplier's products are not listed.
Ryutaro Ariyoshi, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and MTG mutant was purified with size exclusion chromatography using ProteoSEC-D 16/60 6-600 HR (Protein Ark) with PBS (pH 7.4 ...
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No products found
because this supplier's products are not listed.
Seungjae Lee, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Wildtype and mutant CNS were dissected from 1st instar larvae in cold PBS using Dumont #5 forceps (Fine Science Tools). The CNS were fixed in 4% paraformaldehyde in PBS (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Shiqin Xiong, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The AxxQ and FxxQ mutants were generated by the substitution of 27 tyrosines with 27 alanines or phenylalanines using gene synthesis (MCLab) and verified by sequencing and Western blots ...
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No products found
because this supplier's products are not listed.
Radhika Thokala, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Surface expression of EGFR and its mutants was detected by CFP and APC-conjugated cetuximab antibody (Novus Biologicals, Centennial, CO). EGFRvIII expression was detected by anti-EGFRvIII Antibody ...
-
No products found
because this supplier's products are not listed.
Soumita Dutta, et al.,
bioRxiv - Microbiology 2021
Quote:
... Colonies of potential recombinant mutants were screened using colony PCR with 2.0 X Taq RED Master Mix (Genesee Scientific, USA). For colony PCR ...
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No products found
because this supplier's products are not listed.
Akihiro Kawamoto, et al.,
bioRxiv - Microbiology 2020
Quote:
CryoEM images of the wild-type (CCW) and CW mutant basal bodies were collected using a JEM-3200FSC electron cryomicroscope (JEOL) equipped with a liquid-nitrogen cooled specimen stage ...
-
No products found
because this supplier's products are not listed.
Andrew J. Aman, et al.,
bioRxiv - Developmental Biology 2021
Quote:
dio2vp42rc1 and tbx6vp43rc1 mutant lines were produced by injecting in-vitro transcribed single guide RNAs and recombinant Cas9 protein (PNA Bio) as previously described (Saunders et al. ...
-
No products found
because this supplier's products are not listed.
Tatiana Agback, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The synthetic gene blocks encoding mutant nsp1 were ordered from Integrated DNA Technologies and cloned into pE-SUMOpro-3 plasmid (LifeSensors Inc) between Eco RI and Xho I restriction sites ...
-
No products found
because this supplier's products are not listed.
Eric Blanc, et al.,
bioRxiv - Genomics 2019
Quote:
For immunisation 8-12-week old ABabDII mice were injected subcutaneously with 100 µg of mutant short peptide (9-10mers, JPT) supplemented with 50 µg CpG 1826 (TIB Molbiol), emulsified in incomplete Freund’s adjuvant (Sigma) ...
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No products found
because this supplier's products are not listed.
Jun-ichi Kishikawa, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Δnqr cells containing the wild-type or mutant nqr operon on a pBAD plasmid were grown in Luria-Bertani (Miller) medium in 30-liter fermenters (New Brunswick BF-5000 ...
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No products found
because this supplier's products are not listed.
Salli Talvi, et al.,
bioRxiv - Developmental Biology 2021
Quote:
G4 embryonic stem cells derived from 129S6/SvEvTac x C57BL/6NCrl mice (Mutant Mouse Resource & Research Center (MMRRC)) were cultured on neomycin-resistant primary embryonic fibroblast (Neo-resistant MEF feeder cells, Applied StemCell) feeder layer in KnockOut DMEM medium (Gibco ...
-
No products found
because this supplier's products are not listed.
Michael J. Sheedlo, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cag T4SS core complex preparations purified from wild-type or Δcag3 mutant strains were applied to glow discharged Lacey 400 mesh copper grids (TED PELLA) coated with home-made ultra-thin (∼2 nm ...
-
No products found
because this supplier's products are not listed.
Yosef Fichman, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Arabidopsis plants (wild-type and different mutants) were germinated on 0.5X Murashige and Skoog media plates (Caisson Labs, Smithfield, UT, USA). An incision was made in seven-day-old stock seedlings to insert a scion into the cut while keeping the rosette of the stock plant intact ...
-
No products found
because this supplier's products are not listed.
Yasuhiro Hirano, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The mobility of wild-type Bqt4 and its mutants was analyzed using an LSM780 confocal microscope system (Carl Zeiss, Jena, Germany) equipped with a 63× oil-immersion objective lens (NA = 1.4) ...
-
No products found
because this supplier's products are not listed.
Denis Jallet, et al.,
bioRxiv - Microbiology 2024
Quote:
... operated at 200 kV in parallel beam condition using either a K2 (for mutant cells) or K3 (for WT condition) Summit direct electron detector and a BioQuantum energy filter (Gatan Inc.) operated in zero-loss mode with a slit width of 20 eV ...
-
No products found
because this supplier's products are not listed.
Arnaud P. J. Giese, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cochlear explants from Cib2 and Cib3 mutant mice were dissected at postnatal day 5 (P5) and cultured in a glass-bottom petri dish (MatTek, Ashland, MA). They were maintained in Dulbecco’s modified Eagle medium (DMEM ...
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No products found
because this supplier's products are not listed.
Dimitris Dimakis, et al.,
bioRxiv - Genetics 2022
Quote:
Kinetic analysis of wild-type and mutant UapA was measured by estimating uptake rates of [3H]-xanthine uptake (40 Ci mmol-1, Moravek Biochemicals, CA, USA), as previously described in (Krypotou and Diallinas ...
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No products found
because this supplier's products are not listed.
Danira Toral-Rios, et al.,
bioRxiv - Neuroscience 2023
Quote:
... microglia were treated either with endotoxin-free pre-formed fibrils of human recombinant tau-441 (2N4R) P301S mutant protein (0.5μm) expressed with a Baculovirus/sf9 system (StressMarq Biosciences, Cat. No. SPR-471) or PBS in serum-free media ...