-
No products found
Shuai Yan, et al.,
bioRxiv - Physiology 2023
Quote:
Adeno-associated virus serotype 8 expressing mouse Aig1 and AAV8-GFP control were purchased from Applied Biological Materials (Canada). AAV injection was carried out as previously described57 ...
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No products found
because this supplier's products are not listed.
Vamshikrishna Reddy Sammeta, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 300 μl of the lysates were added to 3 mL of Cytoscint (MP Biomedicals) and analyzed by scintillation counting (Beckman LS 6000SC).
-
No products found
because this supplier's products are not listed.
Matthew G. Zimmerman, et al.,
bioRxiv - Microbiology 2019
Quote:
... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Leonard Daly, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Lysate was split equally into 3 wells (80 μl per well) of a white 96 well plate (Greiner), and 200 μl of luciferin working solution added (500 μM Luciferin (Abcam #ab145164) ...
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No products found
because this supplier's products are not listed.
Xiaochun Xie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Level of 2′3′-cGAMP within neural cell lysates after oligomeric Aβ42 treatment was measured by 2′3′-cGAMP competitive ELISA kit (501700, Cayman Chemical) accordingly to the manufacture’s instruction.
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No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Kyoko Tossell, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
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Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...
Cat# LS004185,
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Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Wei Huang, et al.,
bioRxiv - Genetics 2023
Quote:
... 14-3-3 polyclonal antibody (Proteintech, Cat#14503-1-AP), and beta tubulin antibody (Abways Technology ...
-
No products found
because this supplier's products are not listed.
Teodors Pantelejevs, Marko Hyvönen,
bioRxiv - Biochemistry 2022
Quote:
... lysate was loaded on a 3 mL Ni-NTA agarose matrix (Cube Biotech), after which column matrix was washed with 10 CV Nickel Buffer A ...
-
No products found
because this supplier's products are not listed.
Antonios Apostolopoulos, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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No products found
because this supplier's products are not listed.
Azra Lari, Britt A. Glaunsinger,
bioRxiv - Microbiology 2022
Quote:
... and then 1mg of lysate was incubated with prewashed MagStrep “type 3” XT magnetic beads (IBA LifeSciences) overnight in IP buffer (150 mM NaCl ...
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No products found
because this supplier's products are not listed.
K Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Lysates for all WTs within a cohort (Cohort 1: 2F + 3M; Cohort 2: 3M; Cohort 3: 4F; Cohort 4: 6M), and all KOs within a cohort were pooled (Cohort 1 ...
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No products found
because this supplier's products are not listed.
Irina V. Chestakova, et al.,
bioRxiv - Microbiology 2023
Quote:
... and H1N1 virus A/California/07/2009 (Sino Biological) using an arbitrary fluorescence cut-off of 6,000 ...
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No products found
because this supplier's products are not listed.
Christine Vazquez, Chin Yee Tan, Stacy M. Horner,
bioRxiv - Microbiology 2019
Quote:
... and rabbit anti-Sendai virus (SV) (1:1000, MBL International). Secondary antibody incubations were done with Alexa Fluor conjugated antibodies (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Yang S. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysates were sonicated by Covaris Sonicator to ~500 bp fragments (130 μL/tube ...
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No products found
because this supplier's products are not listed.
Rhiannon R. Penkert, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GST lysates were incubated with glutathione agarose resin (GoldBio; 50 μL resin per 0.5-1.5 mL of lysate) for 30 min at 4°C and then washed 6x (3x quick washes ...
-
No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
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No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
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No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
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No products found
because this supplier's products are not listed.
Romina Marone, et al.,
bioRxiv - Bioengineering 2023
Quote:
... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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No products found
because this supplier's products are not listed.
Sylvia Varland, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar) and denatured at 95°C for 5 min ...
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No products found
because this supplier's products are not listed.
Casey L. Mahoney-Crane, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Sections were developed using ImmPACT-3 3’-diaminobenzidine (DAB; Vector Labs), sequentially dehydrated as previously described (Stoyka et al. ...
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No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
Virus injection and optic fiber implantation surgery was performed in C57BL/6J mice (The Jackson Laboratory, #000664) at around P60 ...
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No products found
because this supplier's products are not listed.
Senthil T. Kumar, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 5 mg of 1,2-fioleoyl-sn-glycero-3-phosphoethanolamine (DOPE):1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS):1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) 5:3:2 w/w (Avanti Polar Lipids) were resuspended in 0.8 mL methanol:chloroform 1:1 ...
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No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
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No products found
because this supplier's products are not listed.
José Luis Marín-Rubio, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... using a Gemini C18 column (250 mm × 3 mm, 3 μm, 110 Å; Phenomenex). Buffer A consisted of 20 mM ammonium formate pH 8.0 and Buffer B of 100% acetonitrile ...
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No products found
because this supplier's products are not listed.
Vidur Garg, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
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No products found
because this supplier's products are not listed.
Madalyn M. Won, et al.,
bioRxiv - Microbiology 2023
Quote:
... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel ...
-
No products found
because this supplier's products are not listed.
Xiaonan Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The blood samples from enrolled patients were tested for hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) by Abbott AXSYM HBsAg (normal ...
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No products found
because this supplier's products are not listed.
Bei Zhao, Yinghua Liu, Xuemei Tang, Shi Cheng,
bioRxiv - Cancer Biology 2022
Quote:
... RIPA lysate (Solarbio, Beijing, China), N,N′-methylenebisacrylamide (Aladdin ...
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No products found
because this supplier's products are not listed.
Kuei-Chen Wang, et al.,
bioRxiv - Immunology 2023
Quote:
... or heart tissue lysate (Novus Biologicals) was resolved on 4-20% gradient SDS-PAGE (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Robert M. Cooper, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... 3 μM GSK-3 inhibitor (XVI, Calbiochem; 361559) for the first 3 days.
-
No products found
because this supplier's products are not listed.
Alexander Belyy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ...
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Cat# HY-P1916,
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Yanli Chang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 3mM 3-Methyladenine(3-MA, MedChemExpress, HY-19312) and 80μM dynasore (MedChemExpress,HY-15304) ...
-
No products found
because this supplier's products are not listed.
Andrii Slonchak, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus replication foci were counted using the Image Studio Lite software (v5.2.5, LI-COR, USA), and titres were determined based on dilution factors and expressed as focus forming units per mL (FFU mL^−1).
-
No products found
because this supplier's products are not listed.
Leena Hussein Bajrai, et al.,
bioRxiv - Microbiology 2021
Quote:
... the plates were analyzed by observing virus-induced CPE by light microscope (Nikon-ECLIPSE-Ti), and plaque formation was determined by crystal violet staining (C0775 ...
-
No products found
because this supplier's products are not listed.
Truc Do, et al.,
bioRxiv - Microbiology 2019
Quote:
... 0.6% 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS, Anatrace), 0.5% Triton X-100 reduced ...
-
No products found
because this supplier's products are not listed.
Jia-Shuo Yang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... fluo-3 acetoxymethyl (Fluo-3 thereafter) ester (Biotium, US) following a protocol adapted from (Zhang et al. ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
-
No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
-
No products found
because this supplier's products are not listed.
Timm O. Koller, et al.,
bioRxiv - Biochemistry 2022
Quote:
Grids (Quantifoil R3/3 Cu300 with 3 nm holey carbon) were glow discharged and 4 µL of sample (8 OD260/mL ...
-
No products found
because this supplier's products are not listed.
Susmita Khamrui, et al.,
bioRxiv - Biochemistry 2024
Quote:
... 3-hydroxy-3-methylglutaric acid (HMG) was obtained from TCI or Cayman Chemicals.
-
Building Block
Sold for research purposes only.
Cat# 2592.0, SKU# 2592-1000 mg,
1000mg, US $165.00 / EA, EURO, €150 / EA
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Edinson Lucumi Moreno, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3 M CHIR (Axon Medchem) and 150 M Ascorbic Acid (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Soraya H. Foutouhi, et al.,
bioRxiv - Microbiology 2019
Quote:
... serotype Saint Paul (BCW_88) and serotype Newport (BCW_1378) were grown in Luria-Bertani (LB) broth (Teknova, Holister, CA) and incubated with shaking (200 rpm ...
-
No products found
because this supplier's products are not listed.
Prashant Rajbhandari, et al.,
bioRxiv - Cell Biology 2019
Quote:
Whole cell lysate or tissue lysate was extracted using RIPA lysis buffer (Boston Bioproducts) supplemented with complete protease inhibitor cocktail (Roche) ...
-
No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
-
No products found
because this supplier's products are not listed.
Heejoo Kim, et al.,
bioRxiv - Immunology 2020
Quote:
... Lysates were incubated with 2.5 μg Jmjd1A antibody (Bethyl) and protein-G Dynabeads (ThermoFisher ...