-
No products found
because this supplier's products are not listed.
Galina A. Gusarova, et al.,
bioRxiv - Physiology 2021
Quote:
... and 20 mM HEPES at pH 7.4) supplemented with 4% dextran (70 kDa; TCI America) and 1% FBS (HyClone ...
-
No products found
because this supplier's products are not listed.
Karolina Honzejkova, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 3.5 μL of protein solution was applied to a freshly glow-discharged (45 s total time, Gatan Solarus II 955 (Gatan ...
-
No products found
because this supplier's products are not listed.
Shane Riddell, et al.,
bioRxiv - Immunology 2021
Quote:
... and 501Y.V2.HV001 Passage 4 virus stocks were grown in VeroE6 cells (CCL81; American Type Culture Collection (ATCC), Manassas ...
-
No products found
because this supplier's products are not listed.
Mireia Berdiel-Acer, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... was added for 45 minutes prior to cell acquisition with MetaXpress microscope (Molecular Devices) at the desired time points.
-
No products found
because this supplier's products are not listed.
Qin Yang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... RNP complexes consisting of 4 µM sgRNA (IDT or Synthego) and 3 µM SpyCas9 protein (PNA Bio) were prepared in Buffer R provided by the Neon Transfection System Kit to a volume of 6 µl ...
-
No products found
because this supplier's products are not listed.
Christopher M. Hoel, et al.,
bioRxiv - Biophysics 2022
Quote:
... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Veronika Altmannova, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and contained constant concentration of 45 nM RED-NHS labelled Mer3 (labelling was performed according to the manufacturer’s protocol - Nanotemper Technologies) and increasing concentrations of Top3/Rmi1 ...
-
No products found
because this supplier's products are not listed.
Yuanzhong Zhang, et al.,
bioRxiv - Biophysics 2023
Quote:
... SARS-CoV-2 envelope protein antibody (ProSci; 9169; 1:1000), SARS-CoV-2 Nucleocapsid protein (RayBiotech ...
-
No products found
because this supplier's products are not listed.
Tao Jing, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
-
No products found
because this supplier's products are not listed.
Nia Toshkova, et al.,
bioRxiv - Immunology 2023
Quote:
... measles virus mosaic hemagglutinin (viral proteins were obtained from Immune Technology, New York, NY). Plates were incubated with proteins usually diluted to 5 μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Alexander M Pham, et al.,
bioRxiv - Microbiology 2022
Quote:
... or 45 minutes (AAT Bioquest) at 37ºC ...
-
No products found
because this supplier's products are not listed.
Elizabeth R. Everman, et al.,
bioRxiv - Genetics 2020
Quote:
... flies were homogenized with 3-4 glass beads in 600μL distilled water for 45 seconds using a Mini-Beadbeater-96 (BioSpec Products). Homogenate was centrifuged for 5 minutes at 14,000rpm ...
-
No products found
because this supplier's products are not listed.
David C McKinney, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Protein was concentrated to 10 mg/ml using a Proteus X-spinner with 10 kDa filter (Anatrace) and snap frozen in liquid nitrogen ...
-
No products found
because this supplier's products are not listed.
Valérie Pinarello, et al.,
bioRxiv - Microbiology 2022
Quote:
... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
-
No products found
because this supplier's products are not listed.
Kelly MT Shekiro, et al.,
bioRxiv - Bioengineering 2020
Quote:
... cells were incubated in phenol-red free RPMI with 2 μM calcein-AM and 4 μm ethidium homodimer-1 for 45 min prior to imaging via confocal microscopy (Zeiss NLO LSM 710, Carl Zeiss) where live cells stain green and dead cells are indicated by red nuclear staining.
-
No products found
because this supplier's products are not listed.
Zvi Yaari, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Human epididymis protein 4 (HE4) was purchased from RayBiotech. Human cancer antigen 125 (CA-125) ...
-
No products found
because this supplier's products are not listed.
Laura L Gathercole, et al.,
bioRxiv - Physiology 2021
Quote:
... 45 and 90 minutes (Alphatrak, Abbott, UK).
-
No products found
because this supplier's products are not listed.
Lucia Gonzalo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 45% for IgG-IP (AS09 605, Agrisera) negative control ...
-
No products found
because this supplier's products are not listed.
Simona Notova, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 45% precipitant mix 6 Morpheus II (Molecular Dimensions) for SeMet protein ...
-
Cat# HY-124944-50 mg,
50 mg, USD $1700.0
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Juveriya Qamar Khan, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 45% FBS and 10% DMSO (MedChemExpress # HY-N7060). To test for mycoplasma contamination in all the cell lines ...
-
No products found
because this supplier's products are not listed.
Maria A. Cruz, et al.,
bioRxiv - Cell Biology 2022
Quote:
... envelope (pMD2.G) and packaging (psPAX2) plasmids were amplified in Escherichia coli (GCI-L3, GeneCopoeia) and silica column purified (Qiagen Maxiprep ...
-
No products found
because this supplier's products are not listed.
Christian Schmerling, et al.,
bioRxiv - Microbiology 2024
Quote:
... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...
-
No products found
because this supplier's products are not listed.
Shijie Jin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 45-degree bevel needle on a stereotaxic apparatus (Stoelting). A single dose of AAV5-mCherry-Gfap-Cre (0.5 μL ...
-
No products found
because this supplier's products are not listed.
Paola Munoz-Tello, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
-
No products found
because this supplier's products are not listed.
Kristin Metzdorf, et al.,
bioRxiv - Immunology 2022
Quote:
... 3 μm thick FFPE sections were stained for SARS-CoV-2 with mouse-anti-nucleocapsid CoV-1/2 (Synaptic Systems, HS-452 11, clone 53E2, subtype: IgG2a) and for macrophages with rat-anti-mouse-MAC2 (Biozol Diagnostica/CEDARLANE,CL8942AP ...
-
No products found
because this supplier's products are not listed.
Jesse D. Rogers, et al.,
bioRxiv - Systems Biology 2021
Quote:
... were prepared by mixing 8-arm 40 kDa PEG-Nb with 5 kDa PEG-dithiol crosslinker (JenKem) and 2 mM CRGDS cell adhesive peptide (Bachem) at a 0.99:1 thiol-to-ene ratio in phosphate buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
-
No products found
because this supplier's products are not listed.
Eri Hirata, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 45 μg 16:0-06:0 NBD-PC (Avanti Polar Lipids 810130C) and ∼40,000 cpm [3H]M5-DLO (21 ...
-
No products found
because this supplier's products are not listed.
Kellie A. Heom, et al.,
bioRxiv - Genomics 2023
Quote:
... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
-
No products found
because this supplier's products are not listed.
Anthony J. Asmar, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Protein eluates were dialyzed overnight at 4°C in dialysis buffer (0.1M Tris [pH 8.0], 0.3M NaCl) and concentrated using amicon ultra concentrators ...
-
No products found
because this supplier's products are not listed.
Kim Reid, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay, BMG Labtech POLARstar Omega plate reader).
-
No products found
because this supplier's products are not listed.
Mazdak M. Bradberry, et al.,
bioRxiv - Neuroscience 2022
Quote:
... followed by a second elution with 45 µl 2% n-β-dodecylmaltoside (DDM) (Gold Biotechnologies) for 5 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Larise Oberholster, et al.,
bioRxiv - Neuroscience 2023
Quote:
... consisting of 1 ug/ml of JC virus BIO-PROBE® (Enzo Life Sciences) in 1x In Situ Hybridization buffer (Enzo Life Sciences) ...
-
No products found
because this supplier's products are not listed.
Prashant Kumar, et al.,
bioRxiv - Immunology 2023
Quote:
... a 10 kDa MW internal standard (SDS-MW kit, AB SciEx LLC) was added ...
-
No products found
because this supplier's products are not listed.
Lin-Jie Shu, et al.,
bioRxiv - Plant Biology 2021
Quote:
... accumulation of ROS was monitored for 45 min in a microplate luminometer (Infinite F200 PRO, Tecan, or Luminoskan Ascent 2.1 ...
-
No products found
because this supplier's products are not listed.
Gabriele Ciceri, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Megan L. Norris, Joshua T. Mendell,
bioRxiv - Molecular Biology 2022
Quote:
... 4.5-5 mg total protein were used for each replicate with 4 ug of KIF1C antibody (Bethyl Kif1c/LTXS1 antibody, A301-072A). For western blots ...
-
No products found
because this supplier's products are not listed.
B. Laurent, et al.,
bioRxiv - Microbiology 2020
Quote:
... Chromatographic separation was achieved at 45°C with a Kinetex™ 2.6 μm XB-C18 column (150 × 4.6 mm, Phenomenex, Torrance, USA). Solvent A consisted of methanol/water (10/90 ...
-
No products found
because this supplier's products are not listed.
Yukiko Yamaguchi, et al.,
bioRxiv - Immunology 2022
Quote:
Collected mouse tissue was fixed in 4% paraformaldehyde (4% PFA, Boston BioProducts) and stored in 70% ethanol until processed further ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Joshua E. McGee, et al.,
bioRxiv - Bioengineering 2023
Quote:
... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
-
No products found
because this supplier's products are not listed.
Salime Bazban-Shotorbani, et al.,
bioRxiv - Bioengineering 2021
Quote:
... MAL-PEG-NHS (2 kDa) and m-PEG-NHS (2 kDa) were obtained from Nanocs (USA).
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
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35 mm glass bottom dish with 4 chambers, 20mm microwell, #1 cover glass (0.13-0.16mm). Designed...
Cat# D35C4-20-1-N,
100/case, $184.00
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Akshay Deshpande, et al.,
bioRxiv - Biophysics 2023
Quote:
... 8·104 or 5·105 TZM-bl cells that stably express the emiRFP670-laminB1 nuclear envelope marker was respectively plated in a 8-well chambered slide (#C8-1.5H-N, CellVis) for single time-point nuclear import experiments ...
-
No products found
because this supplier's products are not listed.
Kellie A. Heom, et al.,
bioRxiv - Genomics 2023
Quote:
... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
-
No products found
because this supplier's products are not listed.
Matteo Rossi, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 1:200 in TNB blocking buffer) for 45 min followed by DAPI staining for 5 min (Spectral DAPI, Akoya Biosciences ...
-
No products found
because this supplier's products are not listed.
Sjoerd J. D. Tjalsma, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... the coverslips were incubated with a secondary antibody (anti-rabbit antibodies conjugated with Alexa fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex). Coverslips were then washed three times with PBS for 5 min at RT ...
-
No products found
because this supplier's products are not listed.
Joji Tsunada, Steven J. Eliades,
bioRxiv - Neuroscience 2024
Quote:
... The arrays consist of a 4×4 grid of individually moveable electrodes (4 Mohm tungsten, FHC). Neural signals were sampled at 24 kHz and stored for offline analysis (RA16CH ...
-
No products found
because this supplier's products are not listed.
Michael D. Vahey, Daniel A. Fletcher,
bioRxiv - Microbiology 2019
Quote:
... replacing media with virus growth media supplemented with or without a specified concentration of oseltamivir carboxylate (Toronto Research Chemicals O700980), but without TPCK-treated trypsin ...
-
No products found
because this supplier's products are not listed.
He Liu, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The cytoplasmic and nucleic protein were extracted separately using the protein extraction kit (Boster) according to the manufacture’s introduction ...