-
No products found
because this supplier's products are not listed.
Mitsunori Matsuo, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
-
No products found
because this supplier's products are not listed.
Jens-Uwe Vogel, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... or HCMV 45 kDa late antigen (MBS320051, MyBioSource via Biozol, Echingen, Germany) and were visualized by enhanced chemiluminescence using a commercially available kit (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Alexandra Paul, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
-
No products found
because this supplier's products are not listed.
Maximillian Woodall, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Samples were resolved on NuPAGE 4-12% Bis-Tris Protein Gels with mass standards 10-250 kDa (LI-COR). Proteins were transferred to Immobilon®-FL PVDF membrane (Millipore) ...
-
No products found
because this supplier's products are not listed.
Haneen S. Alsehli, et al.,
bioRxiv - Cell Biology 2023
Quote:
... >98% purity) and 4-arm 10 kDa PEG-NPC (JenKem Technology, USA). The N-terminal primary amine (lysine ...
-
No products found
because this supplier's products are not listed.
Shunqun Luo, et al.,
bioRxiv - Immunology 2021
Quote:
... and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec). Anti-CD24 antibody-bound cells were then magnetically removed ...
-
No products found
because this supplier's products are not listed.
Eric M. Mulhall, et al.,
bioRxiv - Biophysics 2019
Quote:
... pH 7.4) overnight at 4 C in 3.5 kDa MWCO dialysis tubing (Repligen). 1-1.5 mL of Ni2+ Sepharose Excel (GE Life Sciences ...
-
No products found
because this supplier's products are not listed.
Carlos A. Pinzón-Arteaga, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... in 45 % LCDM 45% FBS and 10% DMSO orProFreeze Freezing medium (Lonza, 12-769E) at 2×10^6 cells per ml.
-
No products found
because this supplier's products are not listed.
Xiaozhen Liu, et al.,
bioRxiv - Genetics 2022
Quote:
... and vesicular stomatitis virus G protein (VSV-G) plasmid using polyetherimide (PEI) (B600070, ProteinTech Group, Chicago, USA) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Polly Downton, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Juan Manuel García-Arcos, et al.,
bioRxiv - Biophysics 2022
Quote:
... 20 μM AACOCF3 inhibiting the nuclear envelope stretchsensitive enzyme cPLA2 (Tocris Bioscience #1462), 100 μM 2-APB blocking stretch-activated inositol triphosphate receptors (InsP3Rs ...
-
No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Malika Gantier, et al.,
bioRxiv - Cell Biology 2022
Quote:
... slices were incubated 45 min in Blocking Solution (Vector Laboratories), then with primary rabbit monoclonal anti-human Ku80 antibody (Abcam ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... the SARS-CoV-2 envelope (E) protein (ABclonal RP01263) or the SARS-CoV-2 spike (S ...
-
No products found
because this supplier's products are not listed.
Aaron R. Massey, et al.,
bioRxiv - Neuroscience 2020
Quote:
... rabbit polyclonal antibody to WNV envelope protein (1:800, Novus Biologicals #NB100-56744), and rabbit polyclonal antibody to Iba-1 (1:200 ...
-
No products found
because this supplier's products are not listed.
Zhongyan Lu, et al.,
bioRxiv - Immunology 2021
Quote:
... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
-
No products found
because this supplier's products are not listed.
Maria Guirro, et al.,
bioRxiv - Systems Biology 2019
Quote:
... or a HFD (45% fat, 35% carbohydrate, and 20% protein; D12451, Research Diets, New Brunswick, USA). Two other groups were also fed either a LFD or a HFD for 11 weeks ...
-
No products found
because this supplier's products are not listed.
Yonggyun Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... SB-269970 (a specific inhibitor to 5-HT receptor subtype 7, 5-HT7) was purchased from Cayman Chemical Company (Korea) ...
-
No products found
because this supplier's products are not listed.
Hyeonji Lim, et al.,
bioRxiv - Bioengineering 2023
Quote:
IMR90-4 hiPSCs (passages 45–60) purchased from WiCell were maintained in mTeSR1 (85850, Stem Cell Technologies) in 6-well cell culture plates (30006 ...
-
No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
-
No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
Sodium Hyaluronate (HA, 40 kDa and 100 kDa) was purchased from Lifecore Biomedical LLC (Chaska ...
-
No products found
because this supplier's products are not listed.
Sandy Bauherr, et al.,
bioRxiv - Microbiology 2019
Quote:
... Cells infected with fluorescently labeled virus were fixed using 4 % paraformaldehyde (PFA) in PBS and observed on a Fluoview FV-1000 confocal microscope (Olympus, Japan) to identify fluorescent spots and record their local environment ...
-
No products found
because this supplier's products are not listed.
Anna L. Tyler, et al.,
bioRxiv - Genomics 2022
Quote:
... or a high-fat, high-sucrose (HF/HS) diet (45% fat, 40% carbohydrates, and 15% protein) (Envigo Teklad TD.08811, Envigo, Madison, WI). Mice were maintained on this diet for 26 weeks.
-
No products found
because this supplier's products are not listed.
Ana Maria Porras, et al.,
bioRxiv - Microbiology 2022
Quote:
... 45 male SPF C57BL/6 mice (The Jackson Laboratory) at 7 weeks of age were obtained for this experiment and housed individually during treatments ...
-
No products found
because this supplier's products are not listed.
Senfeng Zhang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Chun Wai Wong, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Proteins were separated on RunBlue 4-12% bis-tris polyacrylamide gels (Expedeon) and then transferred onto iBlot PVDF membranes (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Irene Fernández-Duran, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 20 μg retroviral plasmid were cotransfected with 2.5 μg pCMV-VSVG envelope plasmid and 7.5 μg pUMVC3-gag-pol helper vector using polyethylenimine linear (Alfa Aesar) into HEK293T cells ...
-
No products found
because this supplier's products are not listed.
Théo Crosson, et al.,
bioRxiv - Neuroscience 2023
Quote:
... captured fluorescence emission (515/45 nm) with a 20X objective on a Ti2 microscope (Nikon). Regions of interest (ROI ...
-
No products found
because this supplier's products are not listed.
John B. Finlay, et al.,
bioRxiv - Neuroscience 2022
Quote:
... tissues were incubated with fluorescent conjugated secondary antibodies for 45 minutes (Jackson ImmunoResearch, West Grove, PA). Vectashield with DAPI (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ...
-
No products found
because this supplier's products are not listed.
Komal Raj Rijal, et al.,
bioRxiv - Microbiology 2020
Quote:
... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015; and after 2015, SD Bioline dengue duo (dengue NS1 Ag+ IgG/IgM), Korea ...
-
No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
... S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). SARS-CoV-2 NSP1 (97-095) ...
-
No products found
because this supplier's products are not listed.
Zehra Karakoese, et al.,
bioRxiv - Immunology 2023
Quote:
... were grown for 24 h before adding serial dilutions of our recombinant IFNα subtypes and commercially available IFNα subtypes (PBL Assay Science) for 4.5 h ...
-
No products found
because this supplier's products are not listed.
Franck Raynaud, et al.,
bioRxiv - Biophysics 2021
Quote:
... It is designed to insert spectrophotometric micro-cuvets (10×4×45 mm Sarstedt®) into 8 wells ...
-
No products found
because this supplier's products are not listed.
Zheng Fang, et al.,
bioRxiv - Microbiology 2024
Quote:
... Antibody subtype identification was performed using the SBA ClonotypingTM System/HRP Kit (Southern Biotech, Birmingham, AL, USA). To minimize the background impurities observed during immunoelectron microscopy ...
-
No products found
because this supplier's products are not listed.
Jiajing Hu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... with 4×protein loading buffer (P1016, Solarbio), heated to denature the proteins ...
-
No products found
because this supplier's products are not listed.
Antje Krüger, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... Protein sample was then transferred to midi-size 1 kDa MWCO Tube-O-Dialyzers (G-Biosciences) and first dialyzed against 100 volumes of simplified high-salt buffer with urea overnight ...
-
No products found
because this supplier's products are not listed.
Filippo Bianchini, et al.,
bioRxiv - Immunology 2022
Quote:
... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jeremy F. Brooks, et al.,
bioRxiv - Immunology 2020
Quote:
... with 50μg anti CD40 (FGK-45, BioXCell – NH, USA) on day 0 ...
-
No products found
because this supplier's products are not listed.
Ragna S. Häussler, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The pre-diluted plasma samples and the pre-diluted protein standards (45 μl) were transferred to 5 μl bead stock in an assay plate (Greiner 384-well assay plate) using a liquid handler (SELMA ...
-
No products found
because this supplier's products are not listed.
Andrea Galisova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... according to the manufacturer’s protocol and purified as described previously 45.The ACE2 or DnbALFA proteins were labeled by CF®640R succinimidyl ester dye (Biotium, USA, cat. 92108) in 0.1 M bicarbonate buffer using a 1:4 protein to dye ratio ...
-
No products found
because this supplier's products are not listed.
Alvaro Román-Fernández, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Membranes were incubated for 45 min in Rockland blocking buffer (Rockland) followed by primary antibody (list in Supplementary Table 3 ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Jianhua Yin, et al.,
bioRxiv - Microbiology 2023
Quote:
... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
-
No products found
because this supplier's products are not listed.
R. Novak, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Stage 45 tadpoles were placed in a 24 well μ-Plate 14mm (ibidi, Gräfelfing, Germany), and underwent anesthesia by incubation with 1x tricaine before proceeding to image ...
-
No products found
because this supplier's products are not listed.
Debabrata Dutta, et al.,
bioRxiv - Biophysics 2023
Quote:
... Protochips) glow-discharged for 45 sec at 30 mA using a PELCO easiGlow (Ted Pella). Grids were rear-side-blotted at 25°C and 95% relative humidity using a Leica EM-GP-2 ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...