-
No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
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No products found
because this supplier's products are not listed.
Maria Vittoria Mancini, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
-
No products found
because this supplier's products are not listed.
Duyen Huynh, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the virus envelope plasmid pMD2.G (Addgene #12259) using JetPRIME (Polyplus-transfection ...
-
No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
-
No products found
because this supplier's products are not listed.
Minyue Qiu, et al.,
bioRxiv - Microbiology 2022
Quote:
... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... proteins were concentrated using Amicon Ultra-4 30 kDa centrifugal filter tubes (#UFC803024, Merck) and resuspended in sterile HBSS containing 1.2 mmol/l Ca2+ ...
-
No products found
because this supplier's products are not listed.
Saeko Aoyama-Ishiwatari, et al.,
bioRxiv - Immunology 2020
Quote:
... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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No products found
because this supplier's products are not listed.
S.A. Ford, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Denis G. Knyazev, et al.,
bioRxiv - Biophysics 2023
Quote:
... 4 °C (TLA-45 Beckman rotor). The solubilized proteins were separated on 4–15 % BN-gels and analyzed by immune detection using polyclonal α-YidC antibodies ...
-
No products found
because this supplier's products are not listed.
Carlos A. Elena-Real, et al.,
bioRxiv - Biophysics 2022
Quote:
... Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg, 4°C) (Sartorius). Protein concentrations were determined by means of fluorescence using an sfGFP calibration curve ...
-
No products found
because this supplier's products are not listed.
Michael J. Ricciardi, et al.,
bioRxiv - Immunology 2022
Quote:
... To sequence the envelope from serum virus we isolated viral RNA (Qiagen), synthesized cDNA ...
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No products found
because this supplier's products are not listed.
Jasmine Moshiri, et al.,
bioRxiv - Microbiology 2020
Quote:
... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
-
No products found
because this supplier's products are not listed.
Myka L. Estes, et al.,
bioRxiv - Neuroscience 2019
Quote:
... the anti-MHCI subtype H2-Kb-PE (AF6-88.5) (BD) and a viability marker (Far Red Dead Cell Stain from Invitrogen ...
-
No products found
because this supplier's products are not listed.
Tsukasa Oikawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Retroviruses with the vesicular stomatitis virus–G (VSV-G) envelope were produced by transfection of GP2-293 cells (Clontech) with the pRetroX construct and pVSV-G (Clontech ...
-
No products found
because this supplier's products are not listed.
Juanjuan Wang, et al.,
bioRxiv - Immunology 2022
Quote:
... The recombinant monoclonal antibodies of IgG subtypes were purified after incubation of filtered Expi293F cell supernatant with Protein G resin beads (GenScript) at 4°C overnight ...
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No products found
because this supplier's products are not listed.
Jie Yeap, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Protein molecular ladder (Color Prestained Protein Standard, Broad Range 11–245 kDa, NEB, P7712S). Dry transfer was performed using an iBlot™ 2 gel transfer device (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Sofya A. Polyanskaya, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
-
No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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No products found
because this supplier's products are not listed.
Arpita Dutta, et al.,
bioRxiv - Cell Biology 2023
Quote:
... for 45 min and fixed with 4% Paraformaldehyde (EMS). RPE1 cells were incubated with 5 μM Itraconazole (Selleckchem ...
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No products found
because this supplier's products are not listed.
Joshua E. McGee, et al.,
bioRxiv - Bioengineering 2023
Quote:
... (Human IFN-alpha All Subtype Quantikine ELISA - R&D Systems) (Human IFN-beta DuoSet ELISA R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Iordana A. Shopova, et al.,
bioRxiv - Microbiology 2019
Quote:
... PMNs were sedimented for 10 min with 1000 × g at 4°C on 45° fixed-angle rotor FA-45-30-11 (Eppendorf). The supernatant was filtered by gravity through sterile PVDF 5.0 µm Millex syringe filters (Merck-Millipore) ...
-
No products found
because this supplier's products are not listed.
Elena Erausquin, Jacinto López-Sagaseta,
bioRxiv - Immunology 2021
Quote:
... Protein samples were concentrated using 10 kDa Nanosep columns (Pall Corporation), aliquoted and frozen in liquid nitrogen for storage at −80°C.
-
No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2020
Quote:
... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
-
No products found
because this supplier's products are not listed.
Danny Simpson, et al.,
bioRxiv - Genomics 2023
Quote:
... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
-
No products found
because this supplier's products are not listed.
Payam A. Fathi, Michelle B. Bales, Julio E. Ayala,
bioRxiv - Physiology 2024
Quote:
... for 4 weeks or 45% HFD (4.73 kCal/gram; Research Diets Inc. D12451) for 3 weeks ...
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No products found
because this supplier's products are not listed.
Sam E.J. Paton, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples and protein ladder (10-250 kDa, Cell Signaling Cat. No. 74124) were pipetted into wells of a 18-well Criterion™ TGX Stain- Free™ pre-cast gel (4-15% ...
-
No products found
because this supplier's products are not listed.
Cormac J. Lucas, et al.,
bioRxiv - Immunology 2023
Quote:
... and then stained for 45 min at 4°C with the following antibodies from BioLegend diluted in FACS buffer (PBS with 2% FBS) ...
-
No products found
because this supplier's products are not listed.
Yunlong Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... G*ΔG-VSV virus (VSV G pseudotyped virus, Kerafast) is used to infect 293T cells (American Type Culture Collection [ATCC] ...
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No products found
because this supplier's products are not listed.
Hua Sun, et al.,
bioRxiv - Physiology 2022
Quote:
... followed by (3) surface stripping by incubating with 100 mM Mesna (Santa Cruz, CAS 19767-45-4) at 4°C to remove biotin from uninternalized nephrin ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Xiaoyi Li, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... filtered using 45 um filters and concentrated 100-fold using PEG-it Virus Precipitation Solution (System Biosciences).
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
-
No products found
because this supplier's products are not listed.
Omer Durak, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein samples were run on 4-20% precast polyacrylamide gel (4-20% Mini-PROTEAN TGX Precast Protein Gels, BioRad) to separate them and transferred onto PVDF membranes using semi-dry transfer ...
-
No products found
because this supplier's products are not listed.
Dimitri Ryczko, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
Carlos A. Elena-Real, et al.,
bioRxiv - Biophysics 2022
Quote:
... 150 mM NaCl) at 4°C using SpectraPor 4 MWCO 12-14 kDa dialysis tubing (Spectrum Labs). Dialyzed protein was then concentrated with 10 kDa MWCO Vivaspin centrifugal concentrators (3,500 xg ...
-
No products found
because this supplier's products are not listed.
Judit Vágó, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Equal amounts of protein (3 µg) were loaded into 12–230 kDa separation modules (Protein Simple, Bio-Techne ...
-
No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
-
No products found
because this supplier's products are not listed.
Sergio López-Manzaneda, et al.,
bioRxiv - Bioengineering 2020
Quote:
... bone morphogenetic protein 4 (BMP-4, 6.7 ng/mL; Peprotech), human interleukin 3 (hIL-3 ...
-
No products found
because this supplier's products are not listed.
Linshan Sun, et al.,
bioRxiv - Neuroscience 2021
Quote:
... AAV virus was injected with a glass pipette using an infusion pump (Micro 4, WPI, USA). Viruses were injected bilaterally into target brain areas using the following coordinates ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
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Meredith J. Crane, et al.,
bioRxiv - Immunology 2020
Quote:
... The tissue was incubated for 45 minutes at 37° C in DMEM containing type 4 collagenase (Worthington Biochemical Corporation) and DNAse I (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Tristan Hynes, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... Sucrose pellets (45 mg, Formula P; Bio-Serv) could be delivered at the opposite wall via a dispenser ...
-
No products found
because this supplier's products are not listed.
Cayla N Rodia, et al.,
bioRxiv - Physiology 2019
Quote:
... Administration of 4-5% DSS (Colitis grade, M.W. 35 – 50 kDa, MP Biomedicals) was given ad libitum in drinking water for 5 days ...
-
No products found
because this supplier's products are not listed.
Xuanjun Feng, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Light scattering at 340 nm was monitored at 45°C in a Synergy 4 spectrophotometer (BioTek) for 90min ...
-
No products found
because this supplier's products are not listed.
Sergio López-Manzaneda, et al.,
bioRxiv - Bioengineering 2020
Quote:
... bone morphogenetic protein 4 (BMP-4, 6.7 ng/mL; Peprotech), human interleukin 3 (hIL-3 ...
-
No products found
because this supplier's products are not listed.
Suchitra Joshi, Cedric L Williams, Jaideep Kapur,
bioRxiv - Neuroscience 2022
Quote:
... the animals were habituated to the arena (45 × 45 × 40 cm, Harvard Apparatus) for 10 min for two days (habituation ...
-
No products found
because this supplier's products are not listed.
Céline Christiansen, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a diamond knife (45°, Diatome), collected on copper slot grids and contrasted with 2 % uranyl acetate (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Hiroshi Nishiyama, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Approximately 200-300 nl of virus was injected over 4-6 minutes using a Nanoject II injector (Drummond Scientific). This injection coordinates primarily label the interposed nucleus of the deep cerebellar nuclei ...