-
No products found
because this supplier's products are not listed.
Aniruddha R. Agnihotri, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Spodoptera frugiperda (Sf9) insect cell line was purchased from the National Centre from Cell Sciences (NCCS) cell repository and was maintained in Grace’s insect medium (HiMedia, ...
-
No products found
because this supplier's products are not listed.
Nia Toshkova, et al.,
bioRxiv - Immunology 2023
Quote:
... measles virus mosaic hemagglutinin (viral proteins were obtained from Immune Technology, New York, NY). Plates were incubated with proteins usually diluted to 5 μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Anthony C. Fredericks, et al.,
bioRxiv - Microbiology 2019
Quote:
... at the time of cell seeding using TransIT-insect transfection reagent (Cambridge Biosciences, Cambridge, UK) as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
-
No products found
because this supplier's products are not listed.
Jinsoo Seo, et al.,
bioRxiv - Neuroscience 2020
Quote:
Recombinant human Cdk5 and p25 with N-terminal GST-tags were expressed by Sf9 insect cells via a baculovirus expression system followed by GST affinity purification (BPS Bioscience, San Diego CA).
-
No products found
because this supplier's products are not listed.
Bernarda Calla, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using an E.Z.N.A.® insect DNA kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Hunter K. Walt, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... insects were individually placed into tubes of RNAzol RT reagent (Molecular Research Center, Cincinnati, OH) and frozen at −80°C for total RNA isolation ...
-
No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Anthony Ravussin, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Jason M. Müller, et al.,
bioRxiv - Systems Biology 2023
Quote:
... Asynchronously proliferating cells were metabolically labeled with 4-thiouracil (4sU; Carbosynth) before subcellular fractionation to obtain the nuclear and cytoplasmic RNA fractions or total cellular RNA fraction ...
-
No products found
because this supplier's products are not listed.
Alexey V Sarapulov, et al.,
bioRxiv - Immunology 2020
Quote:
... cells were stained with 4 μg/mL 7-AAD (ABD-17501, Biomol) for live/dead cell discrimination and samples were acquired on BD LSR II equipped with 3 laser lines (405 nm ...
-
No products found
because this supplier's products are not listed.
Lavinia Gambelli, et al.,
bioRxiv - Microbiology 2019
Quote:
... Data were collected using a 4 × 4 k CCD camera or a 4×4 k K2 Summit direct electron detector (Gatan Inc., Pleasanton, USA) running in counting mode ...
-
No products found
because this supplier's products are not listed.
Jonathan Perr, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
-
No products found
because this supplier's products are not listed.
Jennine M. Dawicki-McKenna, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Total protein extraction from cells was performed using 1.5x Laemmli buffer [15% glycerol (Amresco #M152), 3% SDS (Sigma #L5750) ...
-
No products found
because this supplier's products are not listed.
Michael D. Vahey, Daniel A. Fletcher,
bioRxiv - Microbiology 2019
Quote:
... replacing media with virus growth media supplemented with or without a specified concentration of oseltamivir carboxylate (Toronto Research Chemicals O700980), but without TPCK-treated trypsin ...
-
No products found
because this supplier's products are not listed.
Tan Jimin, et al.,
bioRxiv - Microbiology 2020
Quote:
... Annexin 4 (ANXA4) (ABclonal), L-lactate dehydrogenase (LDHA ...
-
No products found
because this supplier's products are not listed.
Brunno R. Levone, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Proteins were quantified using a validated BCA (Bicinchoninic Protein Assay Kit, EuroClone) protocol ...
-
CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor...
Cat# CSC 2F0 V,
1.0 Units, $405.0
Ask
Trevor S. Wendt, Rayna J. Gonzales,
bioRxiv - Physiology 2023
Quote:
... HBMECs at density of 3 to 4 × 105/cm2 were seeded onto glass coverslips coated with attachment factor (Cell Systems; Catalogue number: 4Z0-201) within 6-well plates at passage 7 ...
-
No products found
because this supplier's products are not listed.
Maryann P. Platt, et al.,
bioRxiv - Microbiology 2022
Quote:
... anti-Spn serotype 4 (#16747, Statens Serum Institut) and cleaved-caspase-3 (AF835SP ...
-
No products found
because this supplier's products are not listed.
Leticia R. Q. Souza, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the primary antibodies [Non-structural protein 1 from ZIKV (NS1, 1:500, BioFront Technologies, BF1225-06;), Class III β-tubulin (TuJ3 ...
-
No products found
because this supplier's products are not listed.
Anne-Catherine Fluckiger, et al.,
bioRxiv - Immunology 2019
Quote:
... was used as molecular weight ladder and recombinant ZIKV E and NS1 protein as standard controls (Meridian Life Sciences). Total protein concentration in eVLP samples was determined using Bicinchoninic Acid (BCA ...
-
No products found
because this supplier's products are not listed.
Ennio d’Amico, et al.,
bioRxiv - Biochemistry 2022
Quote:
The following Dynactin coding genes were codon-optimized for protein expression in insect cells and synthesized (Epoch Life Science): DCTN1 (Protein name p150 ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Constructs were amplified from insect cell codon-optimized DNA fragments (Bio Basic Inc., Canada) for protein production in High Five insect cells ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Purified human complement proteins (4 µg/mL, Complement Technology) were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL ...
-
No products found
because this supplier's products are not listed.
Joshua E. McGee, et al.,
bioRxiv - Bioengineering 2023
Quote:
... All saRNA templates were generated from a plasmid encoding NSP1-4 of the Venezuela Equine Encephalitis Virus (VEEV) under either a CleanCap AU (TriLink BioTechnologies) compatible T7 promoter (Promoter seq ...
-
No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... baculovirus gp64 signal sequence under control of the very late polyhedrin promoter was inserted into the insect cell vector plasmid pACEBac1 (Addgene, LGC Standards Teddington, UK) by using another synthetic gene (VK18 ...
-
No products found
because this supplier's products are not listed.
Denisa Bojkova, et al.,
bioRxiv - Microbiology 2022
Quote:
... H1N1 (Influenza A Virus) Nucleoprotein (Bioss, #bs-4976R, 1:4000), ISG15 (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Jieqiong Qu, et al.,
bioRxiv - Microbiology 2023
Quote:
... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
-
No products found
because this supplier's products are not listed.
Bill Ling, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... Cells were lysed by incubation in 4 mL SoluLyse (Genlantis) supplemented with 100 µg DNaseI (Roche ...
-
No products found
because this supplier's products are not listed.
Yue Wen Deng, et al.,
bioRxiv - Microbiology 2023
Quote:
... BL21(DE3) cells were cultured in 4 L Terrific Broth (RPI) media with kanamycin (50 µg/mL ...
-
No products found
because this supplier's products are not listed.
Laiyin Nie, et al.,
bioRxiv - Biochemistry 2020
Quote:
Initial protein crystals were grown at 4°C in condition E10 of the MemGold2-ECO Screen (Molecular Dimensions; 0.05 M Na-acetate pH 4.5 ...
-
No products found
because this supplier's products are not listed.
David Cluet, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HA tagged proteins were labeled overnight at 4°C with the mouse HA.11 Clone 16B12 Monoclonal Antibody (Eurogentec) 1/2000 in PBS + tween 0.2% (v/v ...
-
No products found
because this supplier's products are not listed.
Chelsie A Kadgien, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 500μg of protein at 1μg/pL was rotary-incubated overnight at 4°C with VPS35 antibody (Abnova H00055737-M02) or Mouse IgG2a control antibody (Abcam ab18414 ...
-
WB, IHC,ELISA
Cat# A5444, SKU# A5444-20ul,
20ul, $47.00
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Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
-
No products found
because this supplier's products are not listed.
Joji Tsunada, Steven J. Eliades,
bioRxiv - Neuroscience 2024
Quote:
... The arrays consist of a 4×4 grid of individually moveable electrodes (4 Mohm tungsten, FHC). Neural signals were sampled at 24 kHz and stored for offline analysis (RA16CH ...
-
No products found
because this supplier's products are not listed.
Joshua D. Powell, et al.,
bioRxiv - Microbiology 2024
Quote:
... NJ) and were seronegative to IAV antibodies by a commercial ELISA kit (Swine Influenza Virus Ab Test, IDEXX) prior to the start of the study ...
-
No products found
because this supplier's products are not listed.
Ian Hoskins, et al.,
bioRxiv - Genomics 2023
Quote:
gDNA was extracted from approximately 3-4 million cells with the Cell and Tissue DNA Isolation Kit (Norgen Biotek Corp, 24700), including RNaseA treatment at 37 °C for 15 min ...
-
No products found
because this supplier's products are not listed.
Minji Kim, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 4 ug protein were incubated with 50 uM Suc–Leu–Leu–Val–Tyr– AMC peptide substrate (chymotrypsin-like activity; Bachem, cat. no. I-1395) or 50 uM Ac– NIe–Pro–NIe–Asp–AMC (caspase-like activity ...
-
No products found
because this supplier's products are not listed.
M. Julhasur Rahman, et al.,
bioRxiv - Microbiology 2021
Quote:
The mCherry-E3L integration sites in the purified HGT virus genomes were located by inverse PCR amplification and by PacBio sequencing ...
-
No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
-
No products found
because this supplier's products are not listed.
Weigao Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 300 μL pure protein solution was added to a 1 mm pathlength quartz cuvette (Starna Cells, USA). The scanning wavelength starts from 300 nm and ends at 190 nm ...
-
No products found
because this supplier's products are not listed.
Josh Tycko, et al.,
bioRxiv - Systems Biology 2023
Quote:
... an input of ∼400,000 cells per antibody condition and an overnight antibody incubation at 4°C with H3K4me3 (0.5 μg/ml, EpiCypher), H3K9me3 (0.9 μg/ml ...
-
No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
-
No products found
because this supplier's products are not listed.
Christopher W Fell, et al.,
bioRxiv - Neuroscience 2021
Quote:
... were washed once with PBS and incubated for 4 hours with 100µg/ml fluoresceinamine labelled glycosaminoglycans: 4-O-sulfated chondroitin sulphate (AMS.CSR-FACS-A1, AMSBIO), poly-sulphated chondroitin sulphate (AMS.CSR-FACS-P1 ...
-
No products found
because this supplier's products are not listed.
Iosifina P. Foskolou, et al.,
bioRxiv - Immunology 2022
Quote:
... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
-
No products found
because this supplier's products are not listed.
Basel M. Al-Barghouthi, et al.,
bioRxiv - Genetics 2021
Quote:
... high protein diet (5% fat and 20.3% protein) (D12109C and D12083101, respectively, Research Diets, New Brunswick, NJ), and were euthanized and analyzed at 24–25 weeks of age ...
-
No products found
because this supplier's products are not listed.
Mohamed Abdelgied, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the Opal 4-color Manual ICH kit (NEL810001KT, AKOYA Biosciences) was used according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Shafali Gupta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Rabbit antibodies against the following proteins were used: GAPDH (Trevigen 2275-PC-100; 1:5000), p114 RhoGEF (Abcam ab96520 ...