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No products found
because this supplier's products are not listed.
Xiaozhen Liu, et al.,
bioRxiv - Genetics 2022
Quote:
... and vesicular stomatitis virus G protein (VSV-G) plasmid using polyetherimide (PEI) (B600070, ProteinTech Group, Chicago, USA) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
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No products found
because this supplier's products are not listed.
Sunil Shrestha, et al.,
bioRxiv - Bioengineering 2024
Quote:
... supplemented with 50 ng/mL bone morphogenetic protein 4 (BMP4; Tocris; 314-BP) and 100 ng/mL activin A (Tocris ...
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No products found
because this supplier's products are not listed.
Kyoko Tossell, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
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No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
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No products found
Shuai Yan, et al.,
bioRxiv - Physiology 2023
Quote:
Adeno-associated virus serotype 8 expressing mouse Aig1 and AAV8-GFP control were purchased from Applied Biological Materials (Canada). AAV injection was carried out as previously described57 ...
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No products found
because this supplier's products are not listed.
Kristine Farmen, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The primary antibody used was pneumococcal serotype 4 anti-capsule (SSI Diagnostica, 1:250), secondary antibody used was Alexa Fluor 620 (1:1000) ...
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No products found
because this supplier's products are not listed.
Max Paget, et al.,
bioRxiv - Immunology 2022
Quote:
... MDCK cells expressing IAV-NS1 (MDCK-NS1 cells) were infected with IAVdelNS1 in EMEM containing 0.35% bovine serum albumin (BSA, MP Biomedicals), 4 mM L-glutamine ...
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No products found
because this supplier's products are not listed.
Gabriele Liuzzi, Antonello Mallamaci,
bioRxiv - Developmental Biology 2023
Quote:
- αMecP2 (rat polyclonal IgG2a serotype, Active Motif #61291), 3 μg/reaction.
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No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
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No products found
because this supplier's products are not listed.
Thomas Laval, et al.,
bioRxiv - Immunology 2021
Quote:
... coli serotype 055:B5 TLR grade was purchased from Enzo Life Sciences. Pam3Csk4 was obtained from Invivogen ...
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No products found
because this supplier's products are not listed.
Jessie Altieri, Klemens J. Hertel,
bioRxiv - Molecular Biology 2021
Quote:
... A metabolic labeling protocol was followed[6] and the experimental HEK293 cells were incubated in the presence of 40µM unphosphorylated 4-thiouridine (Cayman Chemical, CAY16373-5). Total RNA was harvested from the cells using Trizol (Ambion ...
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No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
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No products found
because this supplier's products are not listed.
Wadie D. Mahauad-Fernandez, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and 1x premix 4 to 7.3 ampholyte (Protein Simple) were loaded onto the capillaries ...
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No products found
because this supplier's products are not listed.
Sandy Bauherr, et al.,
bioRxiv - Microbiology 2019
Quote:
... Cells infected with fluorescently labeled virus were fixed using 4 % paraformaldehyde (PFA) in PBS and observed on a Fluoview FV-1000 confocal microscope (Olympus, Japan) to identify fluorescent spots and record their local environment ...
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No products found
because this supplier's products are not listed.
Iktae Kim, et al.,
bioRxiv - Biophysics 2022
Quote:
The binding of surface-immobilized NS1 to p85β was measured at 25 °C using an Octet RED biolayer interferometry (Pall ForteBio). The N-terminal His6 and SUMO-tagged NS1 proteins were used for immobilization ...
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No products found
because this supplier's products are not listed.
Chun Wai Wong, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Proteins were separated on RunBlue 4-12% bis-tris polyacrylamide gels (Expedeon) and then transferred onto iBlot PVDF membranes (ThermoFisher ...
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No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
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No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
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No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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No products found
because this supplier's products are not listed.
Tiphaine M.N. Camarasa, et al.,
bioRxiv - Microbiology 2023
Quote:
... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
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No products found
because this supplier's products are not listed.
Lydia Meziani, et al.,
bioRxiv - Immunology 2020
Quote:
... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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No products found
because this supplier's products are not listed.
Maximillian Woodall, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Samples were resolved on NuPAGE 4-12% Bis-Tris Protein Gels with mass standards 10-250 kDa (LI-COR). Proteins were transferred to Immobilon®-FL PVDF membrane (Millipore) ...
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No products found
because this supplier's products are not listed.
Jiajing Hu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... with 4×protein loading buffer (P1016, Solarbio), heated to denature the proteins ...
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No products found
because this supplier's products are not listed.
Justine Creff, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
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No products found
because this supplier's products are not listed.
Nitin T. Supekar, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE).
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No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
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No products found
because this supplier's products are not listed.
Filippo Bianchini, et al.,
bioRxiv - Immunology 2022
Quote:
... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
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No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Alissa Muller, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 1.5 nM bone morphogenetic protein 4 (BMP4, Stemcell technologies) was added to the medium for another three days ...
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No products found
because this supplier's products are not listed.
José Miguel Arcas, et al.,
bioRxiv - Neuroscience 2024
Quote:
Whole-cell voltage- and current-clamp recordings were obtained from mouse DRG neurons or transiently transfected HEK293 cells with borosilicate glass patch-pipettes (Sutter Instruments, 4-8 MΩ resistance) and were performed simultaneously with temperature recordings ...
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35 mm glass bottom dish with 4 chambers, 20mm microwell, #1 cover glass (0.13-0.16mm). Designed...
Cat# D35C4-20-1-N,
100/case, $184.00
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Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
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No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
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No products found
because this supplier's products are not listed.
Jianhua Yin, et al.,
bioRxiv - Microbiology 2023
Quote:
... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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No products found
because this supplier's products are not listed.
Emily A. Hemann, et al.,
bioRxiv - Immunology 2022
Quote:
... this virus/serum mixture is incubated with 0.5% chicken red blood cells (Rockland) and hemagglutination inhibition was measured after 30 minutes.
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Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
Cat# LS004191,
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Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
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No products found
because this supplier's products are not listed.
Qin Yang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... RNP complexes consisting of 4 µM sgRNA (IDT or Synthego) and 3 µM SpyCas9 protein (PNA Bio) were prepared in Buffer R provided by the Neon Transfection System Kit to a volume of 6 µl ...
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No products found
because this supplier's products are not listed.
Paul L. Soto, Christopher D. Morrison,
bioRxiv - Animal Behavior and Cognition 2024
Quote:
... followed by 28 days with a low protein (4% casein, by weight) diet (D11092301, Research Diets Inc). A second group of mice received 21 days exposure to the low protein diet followed by 28 days exposure to the normal protein diet ...
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No products found
because this supplier's products are not listed.
Yanyan Geng, et al.,
bioRxiv - Neuroscience 2022
Quote:
Whole cell recordings were obtained from HEK293 cells using an Axopatch 200B amplifier (Molecular Devices, San Jose, CA). Recordings were filtered at 5 kHz with the amplifier internal filter and digitized at 50 kHz using a Digidata 1550B digitizer (Molecular Devices ...
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No products found
because this supplier's products are not listed.
Tapatee Das, et al.,
bioRxiv - Neuroscience 2020
Quote:
Prior to trypsin digestion all the protein samples were gone through reduction and cysteine blocking using the reagents provided with iTRAQ 4-plex kit (AB SCIEX). Digestion and labelling of proteins were done according to manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Gabriele Ciceri, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
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No products found
because this supplier's products are not listed.
Pin-Ji Lei, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... we inoculated 1×105 PFU UV-inactivated PR8 influenza virus + 2μg Anti-CD40 (BioXCell, Cat: BE0016-2) + 2μg poly I:C (InvivoGen ...
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No products found
because this supplier's products are not listed.
John K. Mich, et al.,
bioRxiv - Neuroscience 2023
Quote:
Virus injection and optic fiber implantation surgery was performed in C57BL/6J mice (The Jackson Laboratory, #000664) at around P60 ...
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No products found
because this supplier's products are not listed.
Nadine Nagy, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
4-MU (Alfa Aesar) was pressed into the mouse chow by TestDiet® and irradiated before shipment ...
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No products found
because this supplier's products are not listed.
Ken Matsuura, et al.,
bioRxiv - Genetics 2021
Quote:
... Protein Concentrate (Vector Laboratories), 0.1% Tween-20 ...
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No products found
because this supplier's products are not listed.
Qiuli Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... was coated overnight at 4°C with 4 µg/mL streptavidin (Jackson ImmunoResearch, Cat#016-000-113) in 100 µL PBS ...
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No products found
because this supplier's products are not listed.
Gabrielle L. Turvey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
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No products found
because this supplier's products are not listed.
Zvi Yaari, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Human epididymis protein 4 (HE4) was purchased from RayBiotech. Human cancer antigen 125 (CA-125) ...