-
No products found
because this supplier's products are not listed.
Nisa P. Williams, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The cells were then cultured with bone morphogenic protein-4 (BMP-4; R&D Systems) and CHIR-99021 in RPMI-B27 medium to specify for cardiac mesoderm lineages ...
-
No products found
because this supplier's products are not listed.
Maria Rojec, et al.,
bioRxiv - Systems Biology 2019
Quote:
... Cell envelopes were disrupted using a Bioruptor Plus sonication system (Diagenode s.a., Belgium) for 10 cycles ...
-
No products found
because this supplier's products are not listed.
Hiroshi Nishiyama, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Approximately 200-300 nl of virus was injected over 4-6 minutes using a Nanoject II injector (Drummond Scientific). This injection coordinates primarily label the interposed nucleus of the deep cerebellar nuclei ...
-
No products found
because this supplier's products are not listed.
Dawei Zhou, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Percentages of virus-infected cells was quantified by using the Gen5 Image+ software (BioTek).
-
No products found
because this supplier's products are not listed.
Juan Manuel García-Arcos, et al.,
bioRxiv - Biophysics 2022
Quote:
... 20 μM AACOCF3 inhibiting the nuclear envelope stretchsensitive enzyme cPLA2 (Tocris Bioscience #1462), 100 μM 2-APB blocking stretch-activated inositol triphosphate receptors (InsP3Rs ...
-
No products found
because this supplier's products are not listed.
Jingyi Guo Fuglstad, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
No products found
because this supplier's products are not listed.
Irene Fernández-Duran, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 20 μg retroviral plasmid were cotransfected with 2.5 μg pCMV-VSVG envelope plasmid and 7.5 μg pUMVC3-gag-pol helper vector using polyethylenimine linear (Alfa Aesar) into HEK293T cells ...
-
No products found
because this supplier's products are not listed.
Xuyong Chen, Lingling Liu, Ruoning Wang,
bioRxiv - Immunology 2022
Quote:
... anti–mIL-4 (8 μg/mL, Bio X Cell), and anti–mIFN-γ (8 μg/mL ...
-
No products found
because this supplier's products are not listed.
Audrey Baylet, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were stimulated with 10 ng/ml hIL-4 (Miltenyi Biotec, Germany) and treated with anti-hIL4 scFv (Creative Biolabs ...
-
No products found
Shuai Yan, et al.,
bioRxiv - Physiology 2023
Quote:
Adeno-associated virus serotype 8 expressing mouse Aig1 and AAV8-GFP control were purchased from Applied Biological Materials (Canada). AAV injection was carried out as previously described57 ...
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No products found
because this supplier's products are not listed.
Kristine Farmen, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The primary antibody used was pneumococcal serotype 4 anti-capsule (SSI Diagnostica, 1:250), secondary antibody used was Alexa Fluor 620 (1:1000) ...
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No products found
because this supplier's products are not listed.
Aaron R. Massey, et al.,
bioRxiv - Neuroscience 2020
Quote:
... rabbit polyclonal antibody to WNV envelope protein (1:800, Novus Biologicals #NB100-56744), and rabbit polyclonal antibody to Iba-1 (1:200 ...
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No products found
because this supplier's products are not listed.
Felix Pahmeier, et al.,
bioRxiv - Microbiology 2020
Quote:
Huh7-Lunet-T7 cells expressing the dengue reporter constructs (Lunet-T7-RC) were seeded onto a glass bottom 35 cm2 dish (Mattek) at a density of 2 × 104 ...
-
No products found
because this supplier's products are not listed.
Gabriele Liuzzi, Antonello Mallamaci,
bioRxiv - Developmental Biology 2023
Quote:
- αMecP2 (rat polyclonal IgG2a serotype, Active Motif #61291), 3 μg/reaction.
-
No products found
because this supplier's products are not listed.
Dominik Saul, et al.,
bioRxiv - Physiology 2021
Quote:
... An Insect Pin (Fine Science Tools, 26001-30 ...
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No products found
because this supplier's products are not listed.
Thomas Laval, et al.,
bioRxiv - Immunology 2021
Quote:
... coli serotype 055:B5 TLR grade was purchased from Enzo Life Sciences. Pam3Csk4 was obtained from Invivogen ...
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No products found
because this supplier's products are not listed.
Gaelen G. Guzman, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Drosophila S2 cells were grown in Schneider’s insect medium with 10% FBS (Cambrex, Rockland, ME) at 27°C in a humidified atmosphere ...
-
No products found
because this supplier's products are not listed.
K.M. Martin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Microscopy of insect cells was performed at 72 hours post-transfection using an Eclipse Ts2R (Nikon) epifluorescent microscope using an S Plan Fluor ELWD 40x/.060 objective with a CFI 10x/22 eyepiece ...
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No products found
because this supplier's products are not listed.
Jiajing Hu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... with 4×protein loading buffer (P1016, Solarbio), heated to denature the proteins ...
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No products found
because this supplier's products are not listed.
Xiaozhen Liu, et al.,
bioRxiv - Genetics 2022
Quote:
... and vesicular stomatitis virus G protein (VSV-G) plasmid using polyetherimide (PEI) (B600070, ProteinTech Group, Chicago, USA) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Robert Bucki, et al.,
bioRxiv - Biophysics 2022
Quote:
... Recombinant human protein arginine deiminase 4 (PAD4) was from Cayman Chemical, Inc.
-
No products found
because this supplier's products are not listed.
Kapil Gupta, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Stitched images of 9 fields covering the central 50% of the well were analyzed for virus infected cells using Cell Reporter Xpress software (Molecular Devices). The cell number was determined by automated counted of DAPI stained nuclei and infected cells were identified as those cells in which positive N staining was detected associated with nuclear DNA staining.
-
No products found
because this supplier's products are not listed.
Holli Carden, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cells were fixed with 4% paraformaldehyde (Calbiochem) for 10 min and permeabilised with 0.1% Triton X-100 for 20 min as previously described32 ...
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35 mm glass bottom dish with 4 chambers, 20mm microwell, #1 cover glass (0.13-0.16mm). Designed...
Cat# D35C4-20-1-N,
100/case, $184.00
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Akshay Deshpande, et al.,
bioRxiv - Biophysics 2023
Quote:
... 8·104 or 5·105 TZM-bl cells that stably express the emiRFP670-laminB1 nuclear envelope marker was respectively plated in a 8-well chambered slide (#C8-1.5H-N, CellVis) for single time-point nuclear import experiments ...
-
No products found
because this supplier's products are not listed.
Kyoko Tossell, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
-
No products found
because this supplier's products are not listed.
Komal Raj Rijal, et al.,
bioRxiv - Microbiology 2020
Quote:
... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015; and after 2015, SD Bioline dengue duo (dengue NS1 Ag+ IgG/IgM), Korea ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... the SARS-CoV-2 envelope (E) protein (ABclonal RP01263) or the SARS-CoV-2 spike (S ...
-
No products found
because this supplier's products are not listed.
Javed Akhter, et al.,
bioRxiv - Microbiology 2020
Quote:
... The recombinant Envelope protein was obtained from MyBioSource (Cat#MBS8309649). All other reagents were from Sigma Aldrich.
-
No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
... S protein (SPN-C52H4) and envelope protein (ENN-C5128) were from Acrobiosystems (Newark, DE). SARS-CoV-2 NSP1 (97-095) ...
-
No products found
because this supplier's products are not listed.
Christine Salaun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Insect cells Sf9 (600100, Oxford Expression Technologies, UK) and Sf9 TriEx (71023-3 ...
-
No products found
because this supplier's products are not listed.
Tao Jing, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Viruses were generated by co-transfecting 107 cells plated the previous day in 15 cm dishes with 30 µg of total plasmid DNA (pNLX.Luc.R-.ΔAvrII and a vesicular stomatitis virus G envelope expressor at 6:1 ratio using PolyJet™ DNA transfection reagent; SignaGen Laboratories). Two days post-transfection ...
-
No products found
because this supplier's products are not listed.
Qian Ma, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
-
No products found
because this supplier's products are not listed.
Zhongyan Lu, et al.,
bioRxiv - Immunology 2021
Quote:
... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
-
No products found
because this supplier's products are not listed.
Gabrielle L. Turvey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... At 48 h post-transfection the supernatant containing virus was harvested and filtered through a low-protein binding filter (0.45µm, Sarstedt) to remove HEK debris ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Wadie D. Mahauad-Fernandez, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and 1x premix 4 to 7.3 ampholyte (Protein Simple) were loaded onto the capillaries ...
-
No products found
because this supplier's products are not listed.
Paula Lagan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Successful virus isolation was confirmed either by haemagglutination of 0.5% chicken blood cells (Envigo #S.B-0008) or by RRT-PCR.
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... Infectivity index was quantified by the ratio of green (virus infected cells) and red (total cells) fluorescence using Image Studio Lite software (LI-COR).
-
No products found
because this supplier's products are not listed.
Xien Yu Chua, Arthur Salomon,
bioRxiv - Biochemistry 2021
Quote:
... Approximately 500,000 cell equivalents or 25 μg of protein was loaded into each well of ClearPAGE 4-20% polyacrylamide gradient gels (Expedeon) and resolved by denaturing polyacrylamide gel electrophoresis at 100 Volts for 1 h ...
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
Guangai Xue, et al.,
bioRxiv - Cell Biology 2021
Quote:
Cells were fixed with 4% paraformaldehyde (Boston Bioproducts) at room temperature (RT ...
-
No products found
because this supplier's products are not listed.
Meng Wang, Xiaoxu Yang, Qixi Wu,
bioRxiv - Genomics 2023
Quote:
... cloni 10G ELITE Electrocompetent Cells (Lucigen 60052-4) in 1mm cuvette (Bio-Rad 1652089 ...
-
No products found
because this supplier's products are not listed.
Warren W. Hom, et al.,
bioRxiv - Cell Biology 2024
Quote:
... cells were fixed with 4% paraformaldehyde (Biotium, 22023). For lysosomal staining ...
-
No products found
because this supplier's products are not listed.
Jessie J.-Y. Chang, et al.,
bioRxiv - Immunology 2023
Quote:
... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... virus particle containing supernatant was collected and enriched using LentiConcentrator (OriGene). Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
-
Dried cells. Suitable lysozyme substrate.
Cat# LS008737,
25 gm, $460.00
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Nawei Sun, et al.,
bioRxiv - Systems Biology 2024
Quote:
... cells were harvested using papain (Worthington, Cat#9001-73-4). Cells were prepared according to 10x Genomics general sample preparation protocol.
-
No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
-
No products found
because this supplier's products are not listed.
Vishruth Girish, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 4 million cells were injected in J:NU mice (Jackson Laboratory, cat. no. 007850). For MCF10A cells expressing HRASG12V ...
-
No products found
because this supplier's products are not listed.
Bryan C. Jensen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... pre-cooled cells were incubated at 4°C with biotinylated tomato lectin (Vector Laboratories) at 2 μg/ml ...