-
No products found
because this supplier's products are not listed.
Ennio d’Amico, et al.,
bioRxiv - Biochemistry 2022
Quote:
The following Dynactin coding genes were codon-optimized for protein expression in insect cells and synthesized (Epoch Life Science): DCTN1 (Protein name p150 ...
-
No products found
because this supplier's products are not listed.
Erick X. Pérez-Guzmán, et al.,
bioRxiv - Microbiology 2019
Quote:
... anti-ZIKV NS1-IgG (Alpha Diagnostics, San Antonio, TX, USA) at baseline ...
-
No products found
because this supplier's products are not listed.
Aniruddha R. Agnihotri, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Spodoptera frugiperda (Sf9) insect cell line was purchased from the National Centre from Cell Sciences (NCCS) cell repository and was maintained in Grace’s insect medium (HiMedia, ...
-
No products found
because this supplier's products are not listed.
Nia Toshkova, et al.,
bioRxiv - Immunology 2023
Quote:
... measles virus mosaic hemagglutinin (viral proteins were obtained from Immune Technology, New York, NY). Plates were incubated with proteins usually diluted to 5 μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Anthony C. Fredericks, et al.,
bioRxiv - Microbiology 2019
Quote:
... at the time of cell seeding using TransIT-insect transfection reagent (Cambridge Biosciences, Cambridge, UK) as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Luciana Galetto, et al.,
bioRxiv - Microbiology 2020
Quote:
... together with 3 μL of Sharpmass VI Prestained Protein Marker (EuroClone) and 5 μL of Unstained SDS-PAGE Standards ...
-
No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
-
No products found
because this supplier's products are not listed.
Thomas J. Cahill, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Macrophages were incubated with 3 ug/mL biotinylated Hyaluronan binding protein (Amsbio) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Jinsoo Seo, et al.,
bioRxiv - Neuroscience 2020
Quote:
Recombinant human Cdk5 and p25 with N-terminal GST-tags were expressed by Sf9 insect cells via a baculovirus expression system followed by GST affinity purification (BPS Bioscience, San Diego CA).
-
No products found
because this supplier's products are not listed.
Bernarda Calla, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using an E.Z.N.A.® insect DNA kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Hunter K. Walt, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... insects were individually placed into tubes of RNAzol RT reagent (Molecular Research Center, Cincinnati, OH) and frozen at −80°C for total RNA isolation ...
-
No products found
because this supplier's products are not listed.
Xiaoyun Ji, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... caspase-3 activity in cell lysates was measured using a Caspase-3 Fluorescence Assay Kit (Biomol Research Laboratories ...
-
No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
-
No products found
because this supplier's products are not listed.
Laura Maria Florez, et al.,
bioRxiv - Immunology 2023
Quote:
... between passages 3 and 7 in complete mouse endothelial cell medium (from Cell Biologics, Euromedex) composed of mouse endothelial cell medium with the addition of endothelial cell medium supplement kit (from Cell Biologics ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Luca Gerosa, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... cells were pulsed for 3 hr with EdU (Lumiprobe, Hunt Valley, MD). After fixing ...
-
No products found
because this supplier's products are not listed.
Barun Mahata, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Next each of 3 100ul aliquots (∼1/3 of each 24 well) of cells were processed for H3K4me3 antibody (Epicypher, #13-0041), H3K27ac antibody (Epicypher ...
-
No products found
because this supplier's products are not listed.
Ada Nowosad, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Lysates (500 µg for HEK293 or 2 mg for U251N) were incubated with 3 µg of indicated antibodies and 12 µl protein-A sepharose beads (IPA300, Repligen) (co-IP ...
-
No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Katelyn Cuttler, et al.,
bioRxiv - Neuroscience 2020
Quote:
... cells were washed 3 times in 1X PBS and blocked in 0.5% BSA (Amresco) in PBS for 20 minutes ...
-
No products found
because this supplier's products are not listed.
Li Sun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were treated with 5μM ATP analog 3-BrB-PP1 (Toronto Research Chemicals; A602985) during 18 °C incubation for 1.5 hr to inactivate Sty1 kinase activity before being collected ...
-
No products found
because this supplier's products are not listed.
Brijesh Kumar, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Transformed cells with 50% basement membrane matrix (BME) type 3 (3632-005-02, Trevigen) (total 100 µL volume ...
-
No products found
because this supplier's products are not listed.
Nurit P. Azouz, et al.,
bioRxiv - Immunology 2023
Quote:
... glycerol) with 250 ng total protein from cell extracts or 120 ng recombinant GST-tagged OVOL1 protein (Abnova #H00005017-P01) and non-fluorescent competitor containing the wild-type or mutant binding site for 30 minutes at room temperature and cross-linked at 120 mJ/cm2 ...
-
No products found
because this supplier's products are not listed.
Claudia Prahst, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... 3% BSA (Nzytech), 0.5% Triton X100 (Sigma) ...
-
No products found
because this supplier's products are not listed.
H. Tai Nguyen, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Western blot analyses of FANC protein expression were performed using total cell protein extracts (∼40 μg from ∼1-2.5 × 106 cells) prepared in RIPA Lysis Buffer (Boster Biological Technology ...
-
No products found
because this supplier's products are not listed.
Teresa Krammer, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... under accession codes GSExxxxxx (FOXA2+ vs FOXA2- cells at Day 5, Figure 3) and GSExxxxxx (FOXA2+ vs FOXA2- cells after BMP perturbations, Figure 4).
-
No products found
because this supplier's products are not listed.
Xun Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... Expi293 cells were grown to 3×106/mL before transfection with the spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
-
CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor...
Cat# CSC 2F0 V,
1.0 Units, $405.0
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Guneet Kaur, et al.,
bioRxiv - Neuroscience 2023
Quote:
Primary human cerebral cortex microvascular endothelial cells (Passage 3, 12 CPD in vitro, ACBRI 376) were procured from Cell Systems (Kirkland, WA 98034, USA). Brain microvascular endothelial cells (BMECs ...
-
No products found
because this supplier's products are not listed.
Samantha J. Ziegler, et al.,
bioRxiv - Biophysics 2024
Quote:
... Grids were blotted for 3 seconds using a CP3 Cryoplunge 3 (Gatan Ametek Inc.) before being plunged into liquid ethane held at –168 °C ...
-
No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
-
No products found
because this supplier's products are not listed.
Jooman Park, et al.,
bioRxiv - Physiology 2023
Quote:
... total proteins from tissue and SVF cells were lysed with RIPA buffer (Boston BioProducts, Boston, MA) supplemented with protease inhibitor cocktail (MedChem Express ...
-
No products found
because this supplier's products are not listed.
Thomas A. Packard, et al.,
bioRxiv - Cell Biology 2021
Quote:
HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
-
No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
-
No products found
because this supplier's products are not listed.
Subhas C. Bera, et al.,
bioRxiv - Biophysics 2021
Quote:
... Model 3 includes dissociation from RPI and is solved in the context of three assumptions for which we have an analytical solution ...
-
No products found
because this supplier's products are not listed.
Leticia R. Q. Souza, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the primary antibodies [Non-structural protein 1 from ZIKV (NS1, 1:500, BioFront Technologies, BF1225-06;), Class III β-tubulin (TuJ3 ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Constructs were amplified from insect cell codon-optimized DNA fragments (Bio Basic Inc., Canada) for protein production in High Five insect cells ...
-
No products found
because this supplier's products are not listed.
Cecilia Martinez Campos, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cells were split and infected with NL4-3 virus for 48h and then pulsed with 100µM of either 4SU (Carbosynth Cat #NT06186) or 6SG (Sigma Cat #858412 ...
-
No products found
because this supplier's products are not listed.
Olga M. Mazina, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 3 µg of normal mouse IgG (protein A purified, Innovative Research) was incubated with 500 µl of diluted WCE (750 µg ...
-
No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... baculovirus gp64 signal sequence under control of the very late polyhedrin promoter was inserted into the insect cell vector plasmid pACEBac1 (Addgene, LGC Standards Teddington, UK) by using another synthetic gene (VK18 ...
-
No products found
because this supplier's products are not listed.
Natalya Leneva, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... with 3 mol% of dipalmitoyl-phosphatidylinositol-3-phosphate (PI(3)P) (Echelon Biosciences) were prepared at a lipid concentration of 0.5 mg/ml in Buffer A by extrusion through a 0.4 μm polycarbonate filter (Avanti Polar Lipids) ...
-
No products found
because this supplier's products are not listed.
Ann Schirin Mirsanaye, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... diluting 3 μl of protein sample (20 nM) in 15 μl PBS buffer loaded on a gasket (Grace Bio-Labs reusable CultureWell gaskets (GBL103250 ...
-
WB, IF,ELISA
Cat# A5199, SKU# A5199-20ul,
20ul, $47.00
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Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
-
No products found
because this supplier's products are not listed.
Sergio Leone, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cell suspensions were sonicated for 3 minutes in 1 ml Covaris tubes (Covaris, 520130) using Covaris S220 with the following settings ...
-
No products found
because this supplier's products are not listed.
Devon L. Moose, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The identity of this cell line as a derivative of PC-3 cells was validated by short tandem repeat (STR) analysis (IDEXX). Cells of both the PC-3 and GS689.Li lines were cultured in DMEM/F12 (Gibco ...
-
No products found
because this supplier's products are not listed.
Ian Hoskins, et al.,
bioRxiv - Genomics 2023
Quote:
gDNA was extracted from approximately 3-4 million cells with the Cell and Tissue DNA Isolation Kit (Norgen Biotek Corp, 24700), including RNaseA treatment at 37 °C for 15 min ...
-
No products found
because this supplier's products are not listed.
Sherine E. Thomas, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Wizard 3&4 (Molecular Dimensions), JCSG +Suite (Molecular Dimensions) ...
-
No products found
because this supplier's products are not listed.
Vidur Garg, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and 3 µM GSK3βi/CHIR99021 (Reprocell) (‘AGi’ media ...
-
No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... J774.A1 cells expressing GFP-LC3A (human) were assayed with IgG coated magnetic beads (ProMag 3 Series-Amine, Bangs Laboratories). The magnetic beads were prepared according to the manufacture’s guidelines ...
-
No products found
because this supplier's products are not listed.
Andreas K Brödel, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... cell cultures were shifted to 37°C for 3 to 6 hours in an orbital shaker at 180 rpm (New Brunswick Innova 44). Samples were centrifuged for 10 min at 4,500g and cell pellets were resuspended and lysed with B-PER reagent (Thermo Scientific ...