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Dengue Virus Serotype 3 NS1 Protein Insect Cells
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Genetex
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in eLife doi: 10.7554/eLife.48469Quote: ... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248). -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Structure doi: 10.1016/j.str.2019.11.005Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ... -
Expression Systems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: Sf9 insect cells (Expression Systems) were grown in suspension in ESF 921 media (Expression Systems ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... The diagnosis of dengue fever was established by the documentation of dengue NS1 antigen (Bioline Dengue NS1 Ag kit ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Circuit-specific viral expression was achieved using the retrograde properties of adeno-associated virus (AAV) serotype 5: AAV5.hSyn.HI.eGFP-Cre.WPRE.SV40 (Addgene; #105540). Sparse labelling was obtained using Cre-inducible ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2020.01456Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ... -
Vector Biolabs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... an adeno-associated virus (AAV) serotype 2 expressing GFP (1010 GU; Vector Biolabs) was injected into layer 2/3 (300µm below the dura ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ... -
GenScript
No products found because this supplier's products are not listed.Cited in The secretory fate of flavivirus NS1 in mosquito cells is influenced by the caveolin binding domainbioRxiv - Molecular Biology 2019Quote: The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.Cited in Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell EntrybioRxiv - Microbiology 2018, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.00856Quote: ... virus-exposed cells were harvested and lysed using Cell Lysis Buffer (Cell Signaling Technology) with cOmplete Protease Inhibitor Cocktail (Sigma ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... cholerae serotype specific antisera (BD Difco) was performed using glass slides to confirm serotypes ... -
Cell Biolabs
No products found because this supplier's products are not listed.Cited in Quantitative single-cell transcriptome-based ranking of engineered AAVs in human retinal explantsbioRxiv - Bioengineering 2021Quote: ... Each AAV serotype was packaged separately using a triple transfection method32using 293AAV cells (Cell Biolabs). AAVs were purified by iodixanol gradient ultracentrifugation ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in Nature doi: 10.1038/s41586-020-1975-8Quote: ... Lysing of cells and particle purification were carried out using AAVpro® Purification Kit (All Serotypes, TAKARA) according to the manufacturer’s instructions ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs) using forward primer OSV 389 and sequential reverse primers OSV 381 ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ... -
Sino Biological
No products found because this supplier's products are not listed.Cited in Structural insights into GlcNAc-1-phosphotransferase that directs lysosomal protein transportbioRxiv - Biochemistry 2021Quote: ... Sf21 insect cells grown in SIM SF medium (Sino Biological Inc.) were used to generate and amplify the baculoviruses ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... neoformans Serotype A strain H99 (American Type Culture Collection (ATCC) 208821) ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Myc (9E10) and pan 14-3-3 (K-19) proteins were from Santa Cruz Biotechnology (Heidelberg ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in mBio doi: 10.1128/mBio.00939-17Quote: ... NS1 substrates were labeled with Tran35S label (Perkin Elmer, Waltham, MA) by incubation in methionine- and cysteine free media containing 10% dialyzed FBS for 20 min before the addition of 200 μCi/mL of Tran35S label ... -
Zymo Research
No products found because this supplier's products are not listed.Cited in Silencing Doublesex expression triggers three-level pheromonal feminization in Nasonia malesbioRxiv - Molecular Biology 2021Quote: ... ZR Tissue & Insect RNA MicroPrep™ (Zymo Research Corp. ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Purified NS1 was then concentrated using Vivaspin columns (Sartorius). -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... The virus was passaged three times in VeroE6 cells and genotype sequence confirmed by Illumina sequencing ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Rescued virus was then amplified via inoculation of virus into 10-day-old embryonated chicken eggs (Charles River) at 37 °C for three days. -
System Biosciences
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Oncotarget doi: 10.18632/oncotarget.24284Quote: HeLa cells were transduced with miRZip Virus Library (MZIPPLVA, System Biosciences), selected for Puromycin resistance and split to single cell dilutions in 96-well plates ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Journal of Biological Chemistry doi: 10.1074/jbc.RA119.008450Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ... -
Peprotech
No products found because this supplier's products are not listed.Cited in Human Deep Cortical Neurons Promote Regeneration and Recovery After Cervical Spinal Cord InjurybioRxiv - Neuroscience 2021Quote: ... Rho-associated protein kinase inhibitor (3 uM; 1293823; PeproTech) was added to the media for 1 day post replating. -
Biotek
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... Percentages of virus-infected cells was quantified by using the Gen5 Image+ software (BioTek). -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... The micropipette was then frontloaded with virus or cells using a controller (WPI, Sys-Micro4). In general ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ... -
ibidi
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... cells were plated onto 3 cm gridded dishes (ibidi) at a density of 125k/dish ... -
Tib Molbiol
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... or dengue virus RNA (TIB Molbiol, Lightmix kit ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... treated insects were dissected under a stereo microscope (SZX9, Olympus, Japan) to count melanized nodule numbers ... -
Greiner
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...