-
No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Michele Martins, et al.,
bioRxiv - Biochemistry 2020
Quote:
... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
-
No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
-
No products found
because this supplier's products are not listed.
Qinrui Wang, et al.,
bioRxiv - Biophysics 2019
Quote:
... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ...
-
No products found
because this supplier's products are not listed.
Maria Vittoria Mancini, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
-
No products found
because this supplier's products are not listed.
Kateryna Nechyporenko, et al.,
bioRxiv - Neuroscience 2024
Quote:
... to express channelrhodopsin (ChR2) or control virus (n = 4, AAV-Ef1a-fDIO-mCherry, 200 mL, 3 × 1012 GC/mL; Serotype: 9; Addgene) was unilaterally injected over 10 minutes into the MePD using a 2-µL Hamilton microsyringe (Esslab ...
-
No products found
because this supplier's products are not listed.
Hanbo Guo, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The protein was expressed in the Sf9 insect cell (Expression system) in the ESF921 medium using the Bac-to-Bac baculovirus system ...
-
No products found
because this supplier's products are not listed.
Minyue Qiu, et al.,
bioRxiv - Microbiology 2022
Quote:
... sections were incubated with anti-dengue virus 1+2+3+4 primary antibodies (ab26837, Abcam, England) at 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Jonas N. Conde, et al.,
bioRxiv - Microbiology 2023
Quote:
... and recLI9-NS1-GFP11 reporter virus was purified using RNeasy kit (Qiagen). cDNA synthesis was performed as above using random hexamer primers (25°C for 10 min ...
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No products found
because this supplier's products are not listed.
Jasmine Moshiri, et al.,
bioRxiv - Microbiology 2020
Quote:
... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
-
No products found
because this supplier's products are not listed.
Alexandre Prola, et al.,
bioRxiv - Physiology 2024
Quote:
... anterior hindlimb compartments of 2/3-months-old C57BL6/J mice were injected with adeno-associated virus serotype 9 (AAV9 – Vector Biolabs) carrying either transgenes for STIM1 (AAV9-CMV-mStim1-2A-eGFP ...
-
No products found
because this supplier's products are not listed.
Sophie M. Jegouic, et al.,
bioRxiv - Microbiology 2020
Quote:
Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
-
No products found
because this supplier's products are not listed.
Wei Zhang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
-
No products found
because this supplier's products are not listed.
Tai L. Ng, et al.,
bioRxiv - Systems Biology 2021
Quote:
... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
S.A. Ford, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Yu Cao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Romel Rosales Ramirez, Juan E. Ludert,
bioRxiv - Molecular Biology 2019
Quote:
The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ...
-
No products found
because this supplier's products are not listed.
Ferdos Abid Ali, et al.,
bioRxiv - Cell Biology 2023
Quote:
Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
-
No products found
because this supplier's products are not listed.
Bolutife Fakoya, et al.,
bioRxiv - Microbiology 2021
Quote:
... cholerae serotype specific antisera (BD Difco) was performed using glass slides to confirm serotypes ...
-
No products found
because this supplier's products are not listed.
Anuttoma Ray, et al.,
bioRxiv - Cell Biology 2023
Quote:
Proteins were extracted from 3-day differentiated SKM cells using cell lysis buffer (9803S, Cell Signaling Technology), supplemented with a complete protease and phosphatase inhibitor cocktail (78442 ...
-
No products found
because this supplier's products are not listed.
Anne Weiß, Ivan Haralampiev, Susann Kummer,
bioRxiv - Microbiology 2022
Quote:
... anti-NS1 (Santa Cruz Biotechnology), and anti-beta-actin (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Charles-Francois V. Latchoumane, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Viruses were produced with Serotype 1 or DJ (Cell Biolabs, Inc.) and purified by CsCl gradients 67 ...
-
No products found
because this supplier's products are not listed.
Fabiana Sassone, et al.,
bioRxiv - Pathology 2023
Quote:
... encoding the AAV serotype 8 capsid and pHelper (Agilent) encoding the AV helper functions ...
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No products found
because this supplier's products are not listed.
Komal Raj Rijal, et al.,
bioRxiv - Microbiology 2020
Quote:
... The data presented in this study represents serological diagnosis using rapid test kit (SD Bioline dengue IgG/IgM antibody up to 2015; and after 2015, SD Bioline dengue duo (dengue NS1 Ag+ IgG/IgM), Korea ...
-
No products found
because this supplier's products are not listed.
Pei Xuan Lee, et al.,
bioRxiv - Microbiology 2020
Quote:
... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Laurie-Anne Lamotte, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... together with Anti-Influenza A Non-Structural Protein 1 (NS1) Mouse Monoclonal Antibody (EMB005, Kerafast). Cells were then washed and incubated with a mixture of secondary antibodies ...
-
No products found
because this supplier's products are not listed.
Elaine Cristina Matos Vicentin, et al.,
bioRxiv - Immunology 2020
Quote:
... of 5 μg of each mAb (αDEC-NS1, αDCIR2-NS1, αDEC or αDCIR2) together with 50 μg poly (I:C) (Invivogen), exactly as described in (17) ...
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No products found
because this supplier's products are not listed.
Sylwia D. Tyrkalska, et al.,
bioRxiv - Immunology 2022
Quote:
Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
-
No products found
because this supplier's products are not listed.
Matthias Höllerhage, et al.,
bioRxiv - Neuroscience 2024
Quote:
Adenoviral vectors of serotype 5 (BioFocus, Charles River Laboratories Nederland B.V. ...
-
No products found
because this supplier's products are not listed.
Conor J. Crawford, et al.,
bioRxiv - Microbiology 2019
Quote:
... neoformans Serotype A strain H99 (American Type Culture Collection (ATCC) 208821) ...
-
No products found
because this supplier's products are not listed.
Pooja Hoovina Venkatesh, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs) using forward primer OSV 389 and sequential reverse primers OSV 381 ...
-
No products found
because this supplier's products are not listed.
Meredith A. Redd, et al.,
bioRxiv - Physiology 2020
Quote:
... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Vincent Basse, et al.,
bioRxiv - Microbiology 2021
Quote:
... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
-
No products found
because this supplier's products are not listed.
Monika Moissidis, et al.,
bioRxiv - Neuroscience 2024
Quote:
Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
-
No products found
because this supplier's products are not listed.
Yidong Wang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... ZR Tissue & Insect RNA MicroPrep™ (Zymo Research Corp. ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Justin A. Spanier, et al.,
bioRxiv - Immunology 2023
Quote:
... Soluble peptide:IAg7 proteins were produced using S2 insect cells as previously described and made into tetramers by conjugating the soluble peptide:IAg7 molecules with BV421 (BioLegend), PE ...
-
No products found
because this supplier's products are not listed.
A.A. Shaldzhyan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
-
No products found
because this supplier's products are not listed.
Yoshitomo Kurogi, et al.,
bioRxiv - Physiology 2022
Quote:
... The dissected tissues were held in a glass-bottom dish (35 × 10 mm, IWAKI #3910-035) with an insect pin (φ0.10 mm, Ento Sphinx Insect Pins) and silicone grease (Beckman), and 20 μL of SDM was added to cover the tissue ...
-
No products found
because this supplier's products are not listed.
Jiejun Zhu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 400nl of virus was delivered to 3 locations respectively by a microinjection system (Nanoliter 2010, WPI Ltd) through a craniotomy (<1 mm2) ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
Jonathan Leis, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
-
No products found
because this supplier's products are not listed.
Fangwu Wang, et al.,
bioRxiv - Immunology 2024
Quote:
... Gene expression and surface protein libraries were generated using the Chromium Next GEM Single Cell 3 ’Reagent Kits v3.1 and sequenced by Illumina MiSeq or NextSeq2000 ...
-
No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
No products found
because this supplier's products are not listed.
S. Gulberk Ozcebe, et al.,
bioRxiv - Bioengineering 2020
Quote:
The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Wei-Hua Chiu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and adeno-associated virus (AAV) particles were injected with a Nanoject 3 (Drummond scientific) at a speed of 1 nl/sec for 30 secs injection with 30 secs pause for a total 33 cycles (i.e. ...
-
No products found
because this supplier's products are not listed.
Sayumi Oishi, et al.,
bioRxiv - Microbiology 2023
Quote:
The insects were dissected under a stereomicroscope (SZ61, Olympus, Japan) in phosphate buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Dawei Zhou, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Percentages of virus-infected cells was quantified by using the Gen5 Image+ software (BioTek).
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Ankita Leekha, et al.,
bioRxiv - Immunology 2022
Quote:
... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...