1 - 50 of 546
suppliers found for
Dengue Virus Serotype 3 NS1 Protein Insect Cells
» view 10000+ matched products
-
Genetex
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in eLife doi: 10.7554/eLife.48469Quote: ... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Structure doi: 10.1016/j.str.2019.11.005Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Journal of Virology doi: 10.1128/JVI.00951-19Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ... -
Expression Systems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: Sf9 insect cells (Expression Systems) were grown in suspension in ESF 921 media (Expression Systems ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... The diagnosis of dengue fever was established by the documentation of dengue NS1 antigen (Bioline Dengue NS1 Ag kit ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2020.01456Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.Cited in Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell EntrybioRxiv - Microbiology 2018, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.00856Quote: ... virus-exposed cells were harvested and lysed using Cell Lysis Buffer (Cell Signaling Technology) with cOmplete Protease Inhibitor Cocktail (Sigma ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: Cre-dependent adeno-associated virus (AAV; serotypes 5 or 9) was used to express GCaMP6s (AAV5-Syn-Flex-GCaMP6s, Addgene), or GCaMP7f (AAV9-Syn-Flex-jGCaMP7f-WPRE ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Pathology 2021Quote: ... Cytopathic effects of the virus were measured after 3 days using the CellTiter-Glo luminescent cell viability assay (Promega), according to the manufacturer’s protocol ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of Virology doi: 10.1128/jvi.01985-18Quote: The PlateliaTM NS1 Ag (Bio-Rad, #72830 Hercules, CA) commercial kit was used to determine the levels of soluble NS1 in cell supernatants collected from silenced ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416, Abcam, WB 1:1000 ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Nature Microbiology doi: 10.1038/s41564-020-0749-2Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNAeasy columns (Qiagen) ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in Nature doi: 10.1038/s41586-020-1975-8Quote: ... Lysing of cells and particle purification were carried out using AAVpro® Purification Kit (All Serotypes, TAKARA) according to the manufacturer’s instructions ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Cell doi: 10.1016/j.cell.2020.09.032Quote: ... Cells transduced with GFP virus were fixed with BD Cytofix (BD Biosciences ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Nature Communications doi: 10.1038/s41467-020-16695-7Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2020, published in Scientific Reports doi: 10.1038/s41598-020-73146-5Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020, published in Virology doi: 10.1016/j.virol.2020.06.007Quote: The HEK 293T virus producer cells were briefly washed with PBS and resuspended using Acutase (Corning) cell suspension solution ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ... -
Agilent
No products found because this supplier's products are not listed.Cited in HIV-1 accessory protein Vpr interacts with REAF and mitigates its associated antiviral activitybioRxiv - Microbiology 2019, published in Journal of Virology doi: 10.1128/JVI.01591-19Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Oncotarget doi: 10.18632/oncotarget.24284Quote: ... Cells were transduced with high titer virus using polybrene (sc-134220, Santa Cruz Biotech) and spinoculated at 800 xg at 32°C for 30 minutes ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Molecular Cell doi: 10.1016/j.molcel.2019.05.015Quote: ... Cells were transduced with concentrated virus and 5μg/ mL polybrene (TR-1003-G, Merck) at 800g for 30 min ... -
Tib Molbiol
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... or dengue virus RNA (TIB Molbiol, Lightmix kit ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... construction and expression in insect cells (GenScript, Piscataway, NJ, USA). Briefly ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in mBio doi: 10.1128/mBio.00939-17Quote: ... NS1 substrates were labeled with Tran35S label (Perkin Elmer, Waltham, MA) by incubation in methionine- and cysteine free media containing 10% dialyzed FBS for 20 min before the addition of 200 μCi/mL of Tran35S label ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies), 500 μg/mL BSA (Sigma Aldrich) ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: ... Virus was pelleted by ultracentrifugation in an SW-28 rotor (Beckman) at 21,000 rpm for 2 hours at 4oC ... -
Cell Biolabs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in eLife doi: 10.7554/eLife.44928Quote: ... pHelper and pAAV-RC (serotype 9 or DJ; purchased from Cell Biolabs Inc., San Diego, CA) using a standard calcium phosphate method ... -
Vector Biolabs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0224527Quote: ... Ad-Laconic (serotype 5) was custom made by Vector Biolabs. LV_Laconic was generated in CECs using Viral Power System from ThermoFisher ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... grubii (serotype A) H99 and RAW264.7 murine monocytic macrophage cell-line were obtained from American Type Culture Collection (ATCC). Environmental strains S48B ... -
Zymo Research
No products found because this supplier's products are not listed.Cited in A hidden gene in astroviruses encodes a cell-permeabilizing protein involved in virus releasebioRxiv - Microbiology 2019Quote: ... at 48 hpe cells were washed 3 times with PBS to eliminate extracellular virus and total RNA was extracted using Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s protocol ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... 50 ng/mL interleukin-3 (IL-3) (these six proteins were purchased from Peprotech, Inc. Rocky Hill, NJ, USA), 2 IU/mL heparin (Ajinomoto Pharmaceutical Co ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in Scientific Reports doi: 10.1038/s41598-018-35231-8Quote: Duplicate samples of mock-infected and Sendai virus-infected cells for 6 hours were used to prepare RNA libraries for sequencing on Illumina HiSeq2000 platform (Illumina) to generate 100 bp paired-end sequencing reads ... -
MyBioSource
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0215708Quote: Recombinant ZKV NS1 (rZKVNS1Ag) antigen expressed by baculovirus in insect cells (MyBioSource Inc, San Diego, USA) was used both to coat the solid-phase where appropriate and ... -
Sino Biological
No products found because this supplier's products are not listed.Cited in Structural insights into GlcNAc-1-phosphotransferase that directs lysosomal protein transportbioRxiv - Biochemistry 2021Quote: ... Sf21 insect cells grown in SIM SF medium (Sino Biological Inc.) were used to generate and amplify the baculoviruses ... -
System Biosciences
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Oncotarget doi: 10.18632/oncotarget.24284Quote: HeLa cells were transduced with miRZip Virus Library (MZIPPLVA, System Biosciences), selected for Puromycin resistance and split to single cell dilutions in 96-well plates ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Cell Reports doi: 10.1016/j.celrep.2019.11.072Quote: Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... The micropipette was then frontloaded with virus or cells using a controller (WPI, Sys-Micro4). In general ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... treated insects were dissected under a stereo microscope (SZX9, Olympus, Japan) to count melanized nodule numbers ... -
Biotek
No products found because this supplier's products are not listed.Cited in Combinatorial deployment of F-actin regulators to build complex 3D actin structures in vivobioRxiv - Cell Biology 2020Quote: ... after genome extraction from larva or adults (E.Z.N.A insect DNA kit, Omega Biotek). -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Scientific Reports doi: 10.1038/s41598-017-17689-0Quote: Subconfluent monolayers (90 % confluency) of A549 cells were infected with Sendai virus (SeV, Cantell strain, Charles River Laboratories) at 5-40 hemagglutinin units (HAU)/106 cells as indicated in serum free medium (SFM) ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2017, published in Development doi: 10.1242/dev.166215Quote: ... Cells are differentiated for 3 days in N2B27 supplemented by 3 µM Chiron99021 (Tocris) and 20 ng/ml bFgf (PeproTech ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in Vitamin D Sufficiency Enhances Differentiation of Patient-Derived Prostate Epithelial OrganoidsbioRxiv - Cell Biology 2020, published in iScience doi: 10.1016/j.isci.2020.101974Quote: ... Protein was quantified using Human Dkk-3 DuoSet Solid Phase Sandwich ELISA (DY1118, R&D Systems, MN), per manufacturer’s instructions ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... 3 × 104 HEK 293T cells grown on 48-well plate (Eppendorf) were co-transfected with (B ... -
ibidi
No products found because this supplier's products are not listed.Cited in In Troyer Syndrome Spartin loss induces Complex I impairments and alters pyruvate metabolismbioRxiv - Cell Biology 2018Quote: ... 3×104 cells were plated in µ-Slide 15 Well (Ibidi, Germany) coated with Poly-D-Lysine (Sigma-Aldrich ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Genetics 2019Quote: ... Virus transduced cells were maintained for 2 weeks under blasticidin (10 μg/ml, Invivogen) selection ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2018Quote: ... stem cell factor (SCF) and FMS-like tyrosine kinase-3 (Flt-3; 2.5 ng ml−1; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) on agarose-coated 24-well plates at 37 °C with 5% CO2 in a water-jacked incubator.