1 - 50 of 562
suppliers found for
Dengue Virus Serotype 2 Envelope Protein Insect Cells
» view 10000+ matched products
-
Genetex
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in eLife doi: 10.7554/eLife.48469Quote: ... Dengue virus Type 2 NS5 protein (Genetex, GTX629447) and GFP (Genetex ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Structure doi: 10.1016/j.str.2019.11.005Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ... -
Addgene
No products found because this supplier's products are not listed.Cited in HLA-C-restricted presentation of a conserved bacterial epitope to an innate NK cell receptorbioRxiv - Immunology 2019, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1903781116Quote: ... The PresentER plasmid encoding a signal peptide from Mouse Mammary Tumor Virus envelope protein followed by the SIINFEKL epitope followed by mCherry was obtained from Addgene (#102945),a kind gift of D ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Journal of Virology doi: 10.1128/JVI.00951-19Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2020.01456Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ... -
Expression Systems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: Sf9 insect cells (Expression Systems) were grown in suspension in ESF 921 media (Expression Systems ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... and pMD.G (vesicular stomatitis virus glycoprotein [VSV-G] envelope plasmid) [53] at a ratio of 1:1:0.5 using FuGENE 6 (Promega) according to the manufacturer’s protocol ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.Cited in Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell EntrybioRxiv - Microbiology 2018, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.00856Quote: ... virus-exposed cells were harvested and lysed using Cell Lysis Buffer (Cell Signaling Technology) with cOmplete Protease Inhibitor Cocktail (Sigma ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in Fascetto Interacting Protein (FIP) Regulates Fascetto (PRC1) to Ensure Proper Cytokinesis and PloidybioRxiv - Cell Biology 2018Quote: Nuclear envelope breakdown (NEB) to anaphase timing was determined based on a sudden change in the nuclear outline and the first detectable separation of sister chromatids respectively ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416, Abcam, WB 1:1000 ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Nature Microbiology doi: 10.1038/s41564-020-0749-2Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNAeasy columns (Qiagen) ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in Nature doi: 10.1038/s41586-020-1975-8Quote: ... Lysing of cells and particle purification were carried out using AAVpro® Purification Kit (All Serotypes, TAKARA) according to the manufacturer’s instructions ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in The Journal of Neuroscience doi: 10.1523/JNEUROSCI.2279-19.2019Quote: ... using an insect pin pushed through the anterior rim of cuticle and into 2 mm of Sylgard (184 Silicone Elastomer, Dow Corning) on the base of a 30 mm diameter petri dish ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... 2 mg of gel filtration protein standard (Bio-Rad 151-1901) was run after sample collection to estimate approximate molecular weight of protein peaks ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... construction and expression in insect cells (GenScript, Piscataway, NJ, USA). Briefly ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2018, published in Scientific Reports doi: 10.1038/s41598-018-27346-9Quote: ... packaging and envelope plasmids (at a ratio 2:1.5:1) using polyethylenimine (PEI; Merck-Millipore). Virus production was enhanced by addition of 10 mM sodium butyrate (Merck-Millipore ... -
Agilent
No products found because this supplier's products are not listed.Cited in HIV-1 accessory protein Vpr interacts with REAF and mitigates its associated antiviral activitybioRxiv - Microbiology 2019, published in Journal of Virology doi: 10.1128/JVI.01591-19Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Cell doi: 10.1016/j.cell.2020.09.032Quote: ... Cells transduced with GFP virus were fixed with BD Cytofix (BD Biosciences ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... Human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography using a MonoQ column (GE Healthcare ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Oncotarget doi: 10.18632/oncotarget.24284Quote: ... Cells were transduced with high titer virus using polybrene (sc-134220, Santa Cruz Biotech) and spinoculated at 800 xg at 32°C for 30 minutes ... -
Tib Molbiol
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... or dengue virus RNA (TIB Molbiol, Lightmix kit ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Epidemiology 2018Quote: ... dengue IgM (Bioline Dengue Duo kit ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... grubii (serotype A) H99 and RAW264.7 murine monocytic macrophage cell-line were obtained from American Type Culture Collection (ATCC). Environmental strains S48B ... -
Beckman
No products found because this supplier's products are not listed.Cited in Automated 3-D mapping of single neurons in the standard brain atlas using single brain slicesbioRxiv - Neuroscience 2018, published in Cell Reports doi: 10.1016/j.celrep.2020.107682Quote: ... The filtered virus supernatant was concentrated using ultracentrifugation at 19,200 rpm for 2 hours (Beckman, SW32 Ti rotor), media was removed from the pellet with viral particles ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Genetics 2019Quote: ... Virus transduced cells were maintained for 2 weeks under blasticidin (10 μg/ml, Invivogen) selection ... -
Cell Biolabs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in eLife doi: 10.7554/eLife.44928Quote: ... pHelper and pAAV-RC (serotype 9 or DJ; purchased from Cell Biolabs Inc., San Diego, CA) using a standard calcium phosphate method ... -
Sino Biological
No products found because this supplier's products are not listed.Cited in Structural insights into GlcNAc-1-phosphotransferase that directs lysosomal protein transportbioRxiv - Biochemistry 2021Quote: ... Sf21 insect cells grown in SIM SF medium (Sino Biological Inc.) were used to generate and amplify the baculoviruses ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... and macrophage inflammatory protein-2 (MIP-2) (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in Journal of Virology doi: 10.1128/JVI.00895-18Quote: ... p24 protein in each virus sample was quantified by using the AlphaLISA HIV p24 Biotin-Free detection kit (Perkin Elmer, Waltham, MA), and input virus was normalized to 5-10ng/ml for the assay ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2017, published in Nature Communications doi: 10.1038/ncomms15665Quote: ... a transcriptional profile of 10T 1/2 fibroblasts transduced with empty virus was generated using RNA-seq analysis (paired-end library layout using Illumina sequencing platform) and a list of all genes with RPKM values below 1.5 compiled using Strand NGS software (Ver ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... heparin (2 μg/ml; Stem Cell Tech, #07980), and Antibiotic-Antimycotic solution (0.1% ... -
Vector Biolabs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0224527Quote: ... Ad-Laconic (serotype 5) was custom made by Vector Biolabs. LV_Laconic was generated in CECs using Viral Power System from ThermoFisher ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... in 2 mL Protein Lo Bind Tubes (Eppendorf) using the following protocol ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2020Quote: ... using ZR Tissue & Insect RNA MicroPrep kit with DNAse treatment (Zymo Research®). RNA purity was analysed with NanoDrop® ND-1000 spectrophotometer (Thermo Fisher Scientific ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019Quote: ... and 106 cells were transferred onto each retronectin/DL1-coated virus-bound well supplemented with 5 ng/mL SCF (Peprotech), 5 ng/mL Flt3-L ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.Cited in A new plant-specific syntaxin-6 protein may define an intracytoplasmic route for begomovirusesbioRxiv - Plant Biology 2020Quote: ... Total protein extracts were obtained from infiltrated leaves expressing the recombinant proteins and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ... -
ibidi
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in eLife doi: 10.7554/elife.40712Quote: HeLa cells were grown in 2-well chamber slides (iBidi 80286), stained with MitoTracker Deep Red ... -
System Biosciences
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Oncotarget doi: 10.18632/oncotarget.24284Quote: HeLa cells were transduced with miRZip Virus Library (MZIPPLVA, System Biosciences), selected for Puromycin resistance and split to single cell dilutions in 96-well plates ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Cell Reports doi: 10.1016/j.celrep.2019.11.072Quote: Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... The micropipette was then frontloaded with virus or cells using a controller (WPI, Sys-Micro4). In general ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... treated insects were dissected under a stereo microscope (SZX9, Olympus, Japan) to count melanized nodule numbers ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Scientific Reports doi: 10.1038/s41598-020-65664-zQuote: ... envelope (pMD2-VSV) and pLenti6/V5 plasmids using linear polyethylenimine (PEI) (23966-1, PolySciences Europe ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Scientific Reports doi: 10.1038/s41598-017-17689-0Quote: Subconfluent monolayers (90 % confluency) of A549 cells were infected with Sendai virus (SeV, Cantell strain, Charles River Laboratories) at 5-40 hemagglutinin units (HAU)/106 cells as indicated in serum free medium (SFM) ... -
Lucigen
No products found because this supplier's products are not listed.bioRxiv - Genomics 2016, published in Nature Methods doi: 10.1038/nmeth.4177Quote: ... coli cells (Lucigen #60242-2) using pre-chilled 1 mm electroporation cuvettes (BioRad #1652089 ... -
PromoCell
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020, published in Lab on a Chip doi: 10.1039/D0LC00738BQuote: ... were cultured at 37°C under 5% CO2 in a sterile humidified incubator in complete growth medium (Endothelial Cell Basal Medium-2 C-22211, supplemented with Endothelial Cell Growth Medium 2 Supplement Pack C-39211, Promocell) and 1X Antibiotic-Antimycotic (Cat ...