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No products found
because this supplier's products are not listed.
Surya Cayre, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The following primary antibodies were used on mouse cell-derived lysates: anti-mouse milk proteins (Accurate Chemical; YNRMMSP; 1/2000), anti-mouse b-casein (a kind gift from C ...
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No products found
because this supplier's products are not listed.
Avinoam Bar-Zion, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Full chemical lysis of cells using SoluLyseTM Protein Extraction Reagent (Genlantis) was used as positive control.
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No products found
because this supplier's products are not listed.
Vitor Mendes, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was mixed in 1:1 and 1:2 (protein to reservoir) ratio with well solution using a mosquito robot (TTP labtech). Initial conditions were obtained in the Classics lite crystallization screen (Qiagen) ...
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No products found
because this supplier's products are not listed.
Ritesh K. Aggarwal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Nuclear protein was then isolated from cells using the EpiQuik Nuclear Extraction Kit (EpiGentek Group Inc.), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Cecilia Martinez Campos, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cells were split and infected with NL4-3 virus for 48h and then pulsed with 100µM of either 4SU (Carbosynth Cat #NT06186) or 6SG (Sigma Cat #858412 ...
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No products found
because this supplier's products are not listed.
Dora Buzas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Control wells containing virus only (no ADAH11) as well as a positive control (a commercial monoclonal antibody (Absolute Antibody; Sb#15) recognising the S protein RBD ...
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No products found
because this supplier's products are not listed.
Ji-Hoon Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... incubated with primary antibody against spike protein diluted 1:5,000 in blocking buffer (Elabscience, E-AB-V1006) overnight in a 37°C incubator with 5% CO2 injection ...
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No products found
because this supplier's products are not listed.
Andrew K.J. Boyce, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Strips were then transferred to blocking buffer containing purified protein of interest: PI(4,5)P2 Grip (1 μg/mL, positive control from Echelon Biosciences), GST (5 μg/mL) ...
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No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
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No products found
because this supplier's products are not listed.
Malgorzata Piechota, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The cells were homogenized with a pre-cooled 1 ml Dounce homogenizer (Wheaton) on ice 10× using a tight pestle ...
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No products found
because this supplier's products are not listed.
Jonathan G Williams, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were then centrifuged and simultaneously stained with 1 x 660-YVAD-FMK (FLICA 660 Caspase-1 assay kit, ImmunoChemistry Technologies) for caspase-1 activation and with fluorochrome-conjugated antibodies CD45-BV421 (clone 30-F11) ...
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No products found
because this supplier's products are not listed.
Jing Zeng, et al.,
bioRxiv - Genetics 2023
Quote:
... 100 ng ml-1 Preclinical Stem Cell Growth Factor (SCF) (CellGenix, cat# 1418- 050) and 100 ng ml-1 Preclinical FMS-like Tyrosine Kinase 3 Ligand (FLT3L ...
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Recombinant HTLV-1 gp46 protein, fused to His-tag, was expressed in HEK293.
Cat# GB46-01VH,
50ug , USD $298
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Yan Qi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the presence of anti- Ad and anti-hemagglutinin antibodies was measured by ELISA against a purified Ad6 virus preparation (Greffex, Inc.) and a recombinant hemagglutinin protein (Creative Biomart). In the first case ...
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No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
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No products found
because this supplier's products are not listed.
Meropi Aravantinou, et al.,
bioRxiv - Immunology 2020
Quote:
... Virus titer was determined in CEMx174 cells (ATCC, Manassass, VA) by p27 ELISA quantification (ZeptoMetrix, Buffalo, NY) and syncytia scoring after 14 days with the calculation method of Reed and Meunch ...
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No products found
because this supplier's products are not listed.
Han Kang Tee, et al.,
bioRxiv - Microbiology 2019
Quote:
... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
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No products found
because this supplier's products are not listed.
Shiwei Liu, et al.,
bioRxiv - Genomics 2023
Quote:
... we grew parasites at 37°C in vitro at 3% hematocrit (serotype A positive human erythrocytes, Valley Biomedical, VA or BioIVT, NY) in RPMI 1640 medium (Invitrogen ...
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No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2020
Quote:
... the original stock of this virus (wild type, WT) was prepared by inoculation of Vero-TMPRSS2 cells with Mynox mycoplasma elimination reagent (Minerva Biolabs) [11] ...
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No products found
because this supplier's products are not listed.
Angela Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... adenovirus (D3Ultra Respiratory Virus Screening & Identification kit, Quidel, San Diego, CA), and cytomegalovirus (D3 DFA Cytomegalovirus Immediate Early Antigen Identification kit ...
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Lenti/Retro Virus
Cat# LVB1000,
Conservation Buffer (1mL), USD $95.00/mL
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
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No products found
because this supplier's products are not listed.
Marwa K. Ibrahim, et al.,
bioRxiv - Microbiology 2021
Quote:
Cells were lysed with PRO-PREP protein extraction solution (Intron Biotechnology). Protein samples were separated on a 12% gel via SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Yabing Nan, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Nuclear proteins were extracted from cells using the ExKine Nuclear and Cytoplasmic Protein Extraction Kit (KTP3001; Abbkine, Wuhan, Hubei, China) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
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No products found
because this supplier's products are not listed.
Liqiao Hu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the cells were collected and subjected to the CAPTUREome S-Palmitoylated Protein Kit (Badrilla). Briefly ...
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No products found
because this supplier's products are not listed.
Mariano Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
Blots were incubated with antibodies (1/3000 dilution) purified on G-protein (Proteogenix, France), followed by horseradish peroxidase-conjugated (HRP ...
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No products found
because this supplier's products are not listed.
Parameswaran Hariharan, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The MSP proteins from the cell lysate were purified with INDIGO Ni-Agarose (Cube biotech) using a protocol as described28,63 ...
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No products found
because this supplier's products are not listed.
Janhavi Prasad Natekar, et al.,
bioRxiv - Microbiology 2022
Quote:
Tissues harvested from virus-inoculated animals were weighed and homogenized in a bullet blender (Next Advance, Averill Park, NY, USA) using stainless steel or zirconium beads ...
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No products found
because this supplier's products are not listed.
Devika Andhare, Michael J Ragusa,
bioRxiv - Biochemistry 2023
Quote:
... Protein expression was induced by adding 1 mM isopropylthio-β-D-galactopyranoside (IPTG; IBI Scientific, IB02125) and the cultures were grown for an additional 18 h at 18°C ...
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No products found
because this supplier's products are not listed.
Kartika Padhan, et al.,
bioRxiv - Immunology 2020
Quote:
... We sorted 1-2 million cells from each cell types for experiments involving FCS2 chamber (Bioptechs) and 100,000-200,00 cells for experiments involving 8-well chamber (Lab-Tek) ...
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No products found
because this supplier's products are not listed.
M Seyfrid, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 1×105 cells in XSFM media (Irvine Scientific, Cat#91141) were activated with anti-CD3/CD28 beads at a 1:1 ratio (Dynabeads ...
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No products found
because this supplier's products are not listed.
Chandima Bulumulla, et al.,
bioRxiv - Neuroscience 2022
Quote:
... cells were seeded in attachment media [1:1 (v/v) plating media to NbActiv4 (BrainBits-NB4)] and cultures were maintained in a 5% CO2 humid incubator at 37°C to facilitate cell adherence ...
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No products found
because this supplier's products are not listed.
Celia Fernandez-Sanz, et al.,
bioRxiv - Physiology 2021
Quote:
Equal amounts of protein (70 μg) supplemented with 5x Protein Loading Buffer (National Diagnostics, USA) were preheated (95°C ...
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No products found
because this supplier's products are not listed.
Anvita Bhargava, et al.,
bioRxiv - Microbiology 2020
Quote:
... cells were incubated with 1 μM of SiR-DNA (Tebu Bio), directly in the culture medium ...
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No products found
because this supplier's products are not listed.
Molly M. Wilson, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The pull-down of ubiquitinated proteins from protein extracts was performed with TUBE 2 agarose beads (LifeSensors), following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Jens O. Watzlawik, et al.,
bioRxiv - Neuroscience 2024
Quote:
... the supernatant of each single B cell well was screened for antigen specificity through direct ELISA for targeting full-length monomeric p-S65-Ub protein (Boston Biochem, U-102), free p-S65-Ub peptides 1 and 2 and BSA-conjugated p-S65-Ub peptides 1 and 2 (provided by 21st Century Biochemicals) ...
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No products found
because this supplier's products are not listed.
Mads Kuhlmann Andersen, et al.,
bioRxiv - Physiology 2022
Quote:
... 5 μL of crude homogenate and protein standards (0 to 2 mg mL-1 bovine serum albumin, ALB001.25, Bioshop Canada, Burlington, ON, CA) was loaded into wells in triplicate followed by 250 μL of Bradford reagent (B6916 ...
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No products found
because this supplier's products are not listed.
Yasunori Watanabe, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein was then stained using methylamine tungstate (Nanoprobes) before being allowed to dry at room temperature for 15 minutes ...
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No products found
because this supplier's products are not listed.
Bao Gia Vu, et al.,
bioRxiv - Genetics 2021
Quote:
... Cells were then lysed with 1 ml glass beads (Scientific Industries Inc, Bohemia, NY) at 4°C for 10 min ...
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No products found
because this supplier's products are not listed.
Viktória Szentgyörgyi, et al.,
bioRxiv - Neuroscience 2024
Quote:
... cells were immunostained with the anti-IIIβ-tubulin (mouse, 1:1000; EXBIO, Czech Republic), anti-GFAP (chicken ...
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No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
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No products found
because this supplier's products are not listed.
Gabriella A. Bertaccini, et al.,
bioRxiv - Biophysics 2023
Quote:
... Cells were resuspended in 1 mL of media CnT-Pr media (Cat. No. CNT-PR, CellnTec) and 1/10th of total cells were plated on 10 µg/mL Fibronectin (Cat ...
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No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Liver proteins were extracted by physical dissociation (Ultra-turrax, IKA) in PEB Buffer (150 mM NaCl ...
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No products found
because this supplier's products are not listed.
Jonard C. Valdoz, et al.,
bioRxiv - Bioengineering 2022
Quote:
... respectively and strained using a 100 µm cell strainer (15-1100-1, Biologix Research Company, MO, USA) twice ...
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No products found
because this supplier's products are not listed.
Alicia Kairouani, et al.,
bioRxiv - Plant Biology 2023
Quote:
10 mg of dried alcohol insoluble cell wall residues were incubated with 10U.ml-1 of endo-1,4-D-xylanase (E-XYLNP) from MEGAZYME previously desalted with centrifulgal filters (Amicon Ultra-0.5ml ...
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No products found
because this supplier's products are not listed.
Numan Ibne Asad, et al.,
bioRxiv - Microbiology 2022
Quote:
Our goal was to model grain quality (protein, gluten, BEM and PMT) using the microbial indicators measured (bacterial and fungal alpha diversity ...
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No products found
because this supplier's products are not listed.
Yirui Li, Julie A. Champion,
bioRxiv - Bioengineering 2024
Quote:
OVA protein vesicles were imaged by a TEM (JEM 100CX-II, JEOL). 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences ...
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No products found
because this supplier's products are not listed.
Paweł P. Knejski, et al.,
bioRxiv - Biochemistry 2023
Quote:
... purified proteins were diluted to 0.05 mg/mL and applied onto plasma-cleaned (Gatan Solarus) copper grids with a continuous carbon layer (Electron Microscopy Sciences) ...
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No products found
because this supplier's products are not listed.
Audrey Tze Ting Khoo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA, 1:2000, MilliporeSigma anti-parvalbumin, 1:2000, Abcam; anti-somatostatin, 1:1000, Peninsula Laboratories) (iii ...
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No products found
because this supplier's products are not listed.
Immacolata Porreca, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... T cells were lysed using DirectPCR (cell) (Viagen Biotech, LA, USA) lysis buffer supplemented with proteinase K (10 µg/mL ...
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No products found
because this supplier's products are not listed.
Alexandre P. Martin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... S6 (effect of ERM proteins on Notch2 signaling) were acquired using a Prime 95B Scientific CMOS camera (Photometrics), giving different signal arbitrary units from the ones recorded with the QuantEMTM 512SC CCD camera used in the other experiments ...