-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Eduardo Olmedillas, et al.,
bioRxiv - Microbiology 2023
Quote:
... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
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No products found
because this supplier's products are not listed.
Takuya Kobayashi, et al.,
bioRxiv - Biochemistry 2021
Quote:
... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
-
No products found
because this supplier's products are not listed.
Maurice Michel, et al.,
bioRxiv - Biochemistry 2024
Quote:
... goat anti-human IgG-Fc Alexa488 (Dianova, 1:400) or goat antihuman IgM Alexa 594 (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Julianne B. Riggs, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Vanessa K.A. Silva, et al.,
bioRxiv - Microbiology 2022
Quote:
... fungal cells were blocked with 1% bovine serum albumin (BSA) for 1h at 37°C and incubated respectively with the primary antibody Fc-Dectin 1 (AdipoGen AG-40B-0138) (5 μg/ml ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Seána Duggan, et al.,
bioRxiv - Microbiology 2019
Quote:
Human neutrophil defensin-1 (hNP-1) (AnaSpec Incorporated, California, USA) susceptibility assay was performed as described previously (15) ...
-
No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 10% FCS (PromoCell, Germany). HEK-NF-κB_luc were supplemented for routine growth with 50 µg/mL Hygromycin B (Invivogen ...
-
No products found
because this supplier's products are not listed.
Ilona Berestjuk, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Human lymphatic fibroblasts (FRC#1 and #2) were purchased from ScienCell Research Laboratories ...
-
No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
-
No products found
because this supplier's products are not listed.
Miquel Rosas-Salvans, et al.,
bioRxiv - Cell Biology 2021
Quote:
... CREST (1:100, human, Antibodies Incorporated, 15-234-0001), Hec1 (1:200 ...
-
No products found
because this supplier's products are not listed.
Valeria Manriquez, et al.,
bioRxiv - Microbiology 2021
Quote:
... and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit ...
-
No products found
because this supplier's products are not listed.
Simon J. Cleary, et al.,
bioRxiv - Immunology 2024
Quote:
... These sequences were codon-optimized and antibodies were expressed as chimeric hIgG1 with or without Fc point mutations in a HEK293 cell system and purified using protein A and buffer exchange (Absolute Antibody).
-
No products found
because this supplier's products are not listed.
Eduardo U. Anaya, et al.,
bioRxiv - Immunology 2020
Quote:
HEK-293 cells expressing Dectin-1A were plated at 40,000 cells in a 35 mm (MatTEK dishes) 24 hours prior to imaging ...
-
No products found
because this supplier's products are not listed.
Jan Fischer, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
-
No products found
because this supplier's products are not listed.
Ethan Iverson, et al.,
bioRxiv - Microbiology 2021
Quote:
... recombinant human IFNβ (1 nM, 11415-1, PBL Assay Science), IFNλ3 (10 nM ...
-
No products found
because this supplier's products are not listed.
Will Macnair, et al.,
bioRxiv - Neuroscience 2023
Quote:
... rabbit anti-human GPR17 (Cayman Chemical, 10136, 1:100), MBP (Rat anti MBP aa82-87 BioRad 1:300) ...
-
No products found
because this supplier's products are not listed.
Yang Liu, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Lin- c-Kit+ cells were sorted into 2%FCS-PBS with 100µM IWR-1 (Tocris, 3532) or control vehicle ...
-
No products found
because this supplier's products are not listed.
Tiffany Russell, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 50 U/mL penicillin/streptomycin and 1% human serum (BioIVT). HSV1 strain Sc16 was used routinely ...
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Marisol Sampedro-Castañeda, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti Cav α1E 1:1000 (Synaptic Systems, 152411, human neurons), rabbit anti CDKL5 1:2000 (Atlas ...
-
No products found
because this supplier's products are not listed.
Sonali A. Gandhi, et al.,
bioRxiv - Biophysics 2023
Quote:
FCS was performed with an inverted microscope (IX71, Olympus) that was customized to resolve up to four fluorophores as described previously.26 The optical setup included a super-continuum laser (SC-Pro ...
-
No products found
because this supplier's products are not listed.
Pawel Stocki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 100 µl of either a 1:5,000 peroxidase-conjugated anti-human Fc antibody or 1:20,000 streptavidin Poly-HRP40 Conjugate (Fitzgerald) diluted in 0.5% BSA in PBST was added and incubated for 1 hr ...
-
No products found
because this supplier's products are not listed.
Daichi Yamasoba, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293-ACE2 cells and HEK293-ACE2/TMPRSS2 cells were stained with rabbit anti-TMPRSS2 polyclonal antibody (BIOSS, Cat# BS-6285R, 1:100). Normal rabbit IgG (SouthernBiotech ...
-
No products found
because this supplier's products are not listed.
Eric E. Gardner, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and Fc receptor blocker reagent (Innovex Biosciences; 1:25). Slides were then briefly washed in TBST and primary antibodies were incubated overnight in blocking buffer at 4C in a humidified chamber ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
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No products found
because this supplier's products are not listed.
Kang Wang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-human SLC7A11(Abclonal, #A13685, 1:1000), anti-human GPX4(Abcam ...
-
No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
-
96 well glass bottom plate. Black polystyrene frame with #1 glass(0.13-0.16mm), with lid,...
Cat# P96-1-N,
20/case, $226.00
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Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
-
No products found
because this supplier's products are not listed.
Loyda M. Morales Rodríguez, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
HEK293 cells were plated onto 25mm coverslips (Electron Microscopy Sciences, #50949050) coated with poly-D-lysine (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Tamina Lebek, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and resuspended in 10% FCS in PBS with either DAPI (1 μM; Biotium, #40043) or DRAQ7 (300 nM ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... 3 ug-mL-1 Human IgM (Innovative Research), a classical pathway activator ...
-
No products found
because this supplier's products are not listed.
Morten S. Hansen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... human GIP (0.1, 1, 10 and 100nM, Bachem), human GIP(3-30)NH2 (10µM ...
-
No products found
because this supplier's products are not listed.
Mirren Charnley, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Fc-DL4 (86 nM; BioVision, P1163), Fc-VCAM-1 (19 nM ...
-
No products found
because this supplier's products are not listed.
MT Heemskerk, et al.,
bioRxiv - Immunology 2021
Quote:
... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...
-
No products found
because this supplier's products are not listed.
Matthew J. Reynolds, et al.,
bioRxiv - Biophysics 2022
Quote:
... Lyophilized human cofilin 1 was purchased from Cytoskeleton (CF01) and reconstituted in MB buffer (20 mM MOPS pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Yu Zhang, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and rabbit-anti-human S100 (1:100, CP021A, Biocare) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Marija Zivaljic, et al.,
bioRxiv - Neuroscience 2022
Quote:
... rabbit anti-human TMPRSS2 (Atlas antibodies HPA035787, 1:1,000), rabbit anti-human actin (Sigma A2066 ...
-
No products found
because this supplier's products are not listed.
Oliver J. Gough, et al.,
bioRxiv - Genomics 2023
Quote:
... Human Negative Control Primer Set 1 (Active Motif, #71001), Human Negative Control Primer Set 3 (Active Motif ...
-
No products found
because this supplier's products are not listed.
Takahiro Yamada, et al.,
bioRxiv - Microbiology 2019
Quote:
... 2 % FCS (MP Biomedicals, Santa Ana, CA), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Natasha S. Kelkar, et al.,
bioRxiv - Immunology 2023
Quote:
... 1:300 dilution of ms-anti-human-C3b (Cedarlane Labs, CL7636AP) was added as primary antibody while goat anti-ms-IgG-PE ...
-
No products found
because this supplier's products are not listed.
Md. Alamgir Hossain, et al.,
bioRxiv - Immunology 2022
Quote:
... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
-
No products found
because this supplier's products are not listed.
Alice Lu-Culligan, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with 20 ng per well of human Syncytin-1 recombinant protein (Abnova #H00030816-Q01) in PBS and were incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Anne-Claire Langlois, et al.,
bioRxiv - Microbiology 2020
Quote:
Human HDL lipoproteins (LP3, Calbiochem) were labeled using the Cy5 monoreactive Dye pack (PA25001 ...
-
No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
-
No products found
because this supplier's products are not listed.
Michael Tellier, et al.,
bioRxiv - Molecular Biology 2021
Quote:
RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...