-
No products found
because this supplier's products are not listed.
Jessica L. Kelliher, et al.,
bioRxiv - Microbiology 2023
Quote:
... Endpoint kinase assays were performed by mixing 3 μg purified PrkA1- 338 with the indicated combinations of ∼3x molar excess of substrate to kinase (6 μg of the generic kinase substrate myelin basic protein [MBP; Novatein Biosciences, Woburn ...
-
No products found
because this supplier's products are not listed.
Jesica Romina Canizo, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... The Large Tumor Suppressor Kinase (LATS) inhibitor (TRULI, Enamine; Z730688380)3,60 and the PKC inhibitor (Gö6983 ...
-
No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... Blocking solution (sciBLOCK Protein D1M solution, Scienion) was added at 200 μL/well with a multichannel pipet and allowed to incubate without agitation for 1 hour ...
-
No products found
because this supplier's products are not listed.
J. Cody Herron, et al.,
bioRxiv - Systems Biology 2022
Quote:
... supplemented with 2% HI-FBS and 20 μM Rho kinase inhibitor Y-27632 (Hello Bio, HB2297) for 25 min of inhibition during frustrated phagocytosis ...
-
No products found
because this supplier's products are not listed.
Rodrigo Muñoz-Castañeda, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A Neurofilament-M antibody (MCA-3H11, Encor Biotechnology) and NeuN antibody (266004 ...
-
No products found
because this supplier's products are not listed.
Marc-Joseph Antonini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and Ag/AgCl electrode (BASi, 3 M NaCl) were used as the counter and reference electrodes ...
-
No products found
because this supplier's products are not listed.
Nicole Fazio, et al.,
bioRxiv - Biophysics 2023
Quote:
... ε260 = 8700 M− 1cm−1 for TMP) and ε260 = 4930 M−1 cm−1 for Cy3 and ε260 = 10000 M−1 cm−1 for Cy5 (Glen Research) based on the sequence of each DNA strand (Supplementary Table S1) ...
-
No products found
because this supplier's products are not listed.
Rudolf O. Schlechter, et al.,
bioRxiv - Microbiology 2023
Quote:
... gas permeability of 0.6 m3 m−2 day−1 and water loss of 1 g m−2 day−1; Brooks Life Sciences, UK), and incubated at 30°C with shaking ...
-
No products found
because this supplier's products are not listed.
Belinda Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... or the insulin receptor signal peptide were synthesized by Eton Biosciences. Similarly ...
-
No products found
because this supplier's products are not listed.
Francesco Caiazza, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and peptides desalted with C18 Desalting Tips (Rainin, Oakland, CA, USA), lyophilized ...
-
No products found
because this supplier's products are not listed.
Zachary R Zeisler, et al.,
bioRxiv - Neuroscience 2023
Quote:
... transferred to a solution of 0.1 M acetic acid (LabChem) with 1% sodium sulfide (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Mallory Genest, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
-
No products found
because this supplier's products are not listed.
Asha Mary Joseph, et al.,
bioRxiv - Microbiology 2021
Quote:
... appropriate volume of 0.5 mg/ml MMC (AG Scientific, #M-2715) stock (prepared by resuspending in sterile water ...
-
No products found
because this supplier's products are not listed.
Robert C. Hurt, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Cells were lysed by adding 400 μL of Solulyse-M (Genlantis) supplemented with 25 units/mL Benzonase Nuclease (Novagen ...
-
No products found
because this supplier's products are not listed.
Sudha Silwal Gautam, et al.,
bioRxiv - Bioengineering 2020
Quote:
... calcitonin gene-related peptide (CRGP, 1:800, guinea pig polyclonal; Progen Biotechnik GmbH), or vascular endothelial cell marker von Willebrand factor (vWF ...
-
No products found
because this supplier's products are not listed.
Daniel Pokorny, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Both wild-type and kinase-dead (D286A) Sgk3 were expressed in baculvirus-infected Sf9 insect cells grown in ESF 921 medium (Expression Systems) at 27°C ...
-
No products found
because this supplier's products are not listed.
Pavel A. Makhnovskii, et al.,
bioRxiv - Physiology 2023
Quote:
... with 1% beta-mercaptoethanol in a 1.5-ml tube using a polypropylene pestle and a drill and then incubated (55 °C, 10 min) with protein kinase K (Evrogen, Russia). Total RNA was extracted by a silica spin column (CleanRNA Standard ...
-
No products found
because this supplier's products are not listed.
Michael F. Coleman, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Mice used in M-Wnt studies were fed AIN-93G (Research Diets). Mice used in 4T1-PC-FF studies were fed standard chow diet (Teklad Global 2018S ...
-
No products found
because this supplier's products are not listed.
Xuan Yu, et al.,
bioRxiv - Microbiology 2022
Quote:
... tabacinus LZ-M was observed by scanning electron microscopy (JEOL JSM-IT500LA). Spectrophotometry was used to measure the concentrations of AR73 ...
-
No products found
because this supplier's products are not listed.
Tyson J. Ruetz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10] ...
-
No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
... mice were treated with 2 mg of an Ifnar1-blocking antibody (MAR1-5A3, Leinco Technologies) by i.p ...
-
No products found
because this supplier's products are not listed.
Julia M. Gauglitz, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Hexakis (m/z 622.029509; (1H, 1H, 2H difluoroethoxy)phosphazene (Synquest Laboratories, Alachua, FL)) was used for lock mass correction ...
-
No products found
Jan-Renier A.J. Moonen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
PAEC were transduced with adenoviral constructs encoding a constitutive active mutant of dual specificity mitogen-activated protein kinase 5 (caMEK5) (#000101A, Applied Biological Materials Inc); Flag-tagged KLF4 (#VH829440 ...
-
No products found
because this supplier's products are not listed.
Michael T. Maloney, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The resulting graphs for m/z 865.502 (corresponding to BMP 22:6/22:6) and m/z 834.530 (corresponding to PS 40:6 ...
-
No products found
because this supplier's products are not listed.
Peter Verstraelen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Permeabilization was done in blocking buffer (0.1% bovine serum albumin, 10% normal horse serum (Innovative Research IGHSSER) in PBS ...
-
No products found
because this supplier's products are not listed.
Chiara Aloise, et al.,
bioRxiv - Microbiology 2023
Quote:
... The cells were then incubated in blocking buffer containing mouse anti-dsRNA (1:1000; English & Scientific Consulting), goat anti-eIF3η (1:200 ...
-
No products found
because this supplier's products are not listed.
Hsin Yao Chiu, Amos Hong Pheng Loh, Reshma Taneja,
bioRxiv - Cancer Biology 2021
Quote:
... (NC, USA) and cultured in skeletal muscle cell growth medium (#SKM-M, Zen-Bio, USA). Cells were maintained at a confluency of no more than 70% and experiments were performed with cells under 7 passages ...
-
No products found
because this supplier's products are not listed.
Bill Ling, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The CuAAC reaction was prepared by combining 22 mg m-PEG-azide (10 kDa, BroadPharm), 44 µL DMSO ...
-
No products found
because this supplier's products are not listed.
The Nhu Nguyen, et al.,
bioRxiv - Microbiology 2023
Quote:
Antibody responses against IAV-S nucleoprotein (NP) were measured by using a commercial blocking ELISA (IDEXX, Montpellier, France), following the manufacturer’s recommendation ...
-
No products found
Bocheng Yin, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The stock solution of 0.5 M biotin-dPEG®3-benzophenone (biotin-BP, 10267, Quanta BioDesign) in anhydrous DMSO (89139-666 ...
-
No products found
because this supplier's products are not listed.
Salime Bazban-Shotorbani, et al.,
bioRxiv - Bioengineering 2021
Quote:
... MAL-PEG-NHS (2 kDa) and m-PEG-NHS (2 kDa) were obtained from Nanocs (USA).
-
No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... biotinylated flagellin peptides were conjugated on to Streptavidin coated 4um polystyrene beads (Spherotech, Inc. Cat # PAK-4067-8K). Beads conjugated with each individual peptide have different fluorescent intensity in the APC/Cy7 channel so that they can be differentiated from each other by flow cytometry ...
-
No products found
because this supplier's products are not listed.
Kelsey A. Nolden, Megan C. Harwig, R. Blake Hill,
bioRxiv - Biochemistry 2023
Quote:
... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
-
No products found
Katrina B. Velle, et al.,
bioRxiv - Cell Biology 2023
Quote:
... ~3X105 NEG-M cells were seeded into a 6-well glass-bottom plate (Cellvis, P06-1.5H-N). FM™ 4-64 Dye (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Satya Omprabha, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... The nature of NPs was determined using multipurpose X-ray diffraction (M-XRD, Rigaku® SmartLab 9KW). The optical properties of the NPs were analyzed using a UV-VIS spectrophotometer (Analytika Jena SPECORD® 210 PLUS ...
-
No products found
because this supplier's products are not listed.
Colton D. Payne, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The resin used as an anchor for peptide assembly was Tentagel XV 4-hydroxymethyl phenoxyacetic acid (Rapp Polymere, GmbH). Prior to the loading of the C-terminal residue ...
-
No products found
because this supplier's products are not listed.
Jhansi L. Leslie, et al.,
bioRxiv - Microbiology 2019
Quote:
... each plate had a positive control consisting of toxin coated wells reacted with mouse TcdA monoclonal antibody TGC2 diluted 1:5,000 in blocking buffer (antibodies-online.com ABIN335169). The optical density at 410nm and 650nm was recorded on a VersaMax plate reader (Molecular Devices ...
-
No products found
because this supplier's products are not listed.
Barr Tivon, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 4 µl of 20 mM alkyne-peptide in DMSO were mixed with 10 µl of 5 mM BDP-TMR-azide (Lumiprobe). To the mixture was added 50 µl water ...
-
No products found
because this supplier's products are not listed.
Takafumi Kato, et al.,
bioRxiv - Pathology 2021
Quote:
... PRR4 cDNA without a signal peptide sequence (corresponding to amino acids 17 to 134) was cloned into the pM-secSUMOstar Vector (7121, LifeSensors). SUMOstar-PRR4 vectors were transfected into Expi293 cells (1 mg of DNA per liter of transfection ...
-
No products found
because this supplier's products are not listed.
Fei Yang, et al.,
bioRxiv - Pathology 2023
Quote:
... overnight at 4℃ in a blocking solution and then with colloidal gold-conjugated anti-rabbit IgG (1.4-nm diameter; 1:200; #2003, Nanoprobes, Yaphank, NY, USA) in a blocking solution for 2 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Kaarjel K. Narayanasamy, et al.,
bioRxiv - Biophysics 2021
Quote:
... Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek) pre-coated with 30 µg/ml of fibronectin from human plasma (Sigma ...
-
No products found
because this supplier's products are not listed.
Erin M. Euliano, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Near-infrared fluorescent peptides were made by coupling 2 equivalents N-hydroxysuccinimide (NHS) ester-functionalized ATTO 647N (ATTO-TEC, Siegen, Germany) to the N-terminus with 4 equivalents of DIEA overnight protected from light ...
-
No products found
because this supplier's products are not listed.
Toshiharu Ichinose, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The ribosome-bound mRNA was eluted with 50 µl of 100 µg/ml 3× FLAG peptide (GEN-3XFLAG- 25, Protein Ark) dissolved in the lysis buffer.
-
No products found
because this supplier's products are not listed.
Jonathan M Budzik, et al.,
bioRxiv - Microbiology 2019
Quote:
Immunostaining for LC3 was performed with blocking and permeabilization buffer (0.3% Triton X-100, 2% bovine serum albumin) and anti-LC3 (clone 2G6, Nanotools #0260-100/LC3-2G6) at a dilution of 1:200 for 3 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Luca Pesce, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Blocks were embedded in low melting agarose (4% in 0.01 M PBS) and cut into 450±50 μm-thick coronal sections with a vibratome (Vibratome 1000 Plus, Intracel, UK). After cutting ...
-
No products found
because this supplier's products are not listed.
Roger D. Latham, et al.,
bioRxiv - Biochemistry 2019
Quote:
... of ferriprotoporphyrin IX were prepared fresh by dissolution in 0.1 M sodium hydroxide of either bovine hemin chloride (ferriprotporphyrin IX chloride, Frontier Scientific), or porcine hematin (ferriprotoporphyrin IX hydroxide ...
-
No products found
because this supplier's products are not listed.
James F. Pelletier, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... using reactive ion etching to define 3.1 µm deep chambers arrayed along a 100 µm wide by 22 µm deep channel defined by SU-8 photoresist (MicroChem). After treating with a silane release layer ...
-
No products found
because this supplier's products are not listed.
Yury A. Nikolaev, et al.,
bioRxiv - Neuroscience 2023
Quote:
The bill skin samples were then sectioned into 300 µm thick slices in 0.13 M cacodylate buffer using a Compresstome (Precisionary, MA). The slices were washed in cacodylate buffer (0.13 M) ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Khaled Youssef, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and 1 mL 1 M MgSO4 in 1 L distilled water) using a RotoFlex™ tube rotator (RK-04397-40, Cole-Parmer, Canada). L1 larvae were collected by centrifuging and seeded on top of NGM plates ...