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No products found
because this supplier's products are not listed.
Matthew D. Romero, Rey A. Carabeo,
bioRxiv - Cell Biology 2021
Quote:
... rinsed with HBSS and labeled with rabbit polyclonal anti-Chlamydia trachomatis antibody (Abcam ab252762, Cambridge, MA, USA). Cells were then rinsed in 1x PBS and labeled with Alexa Fluor 488 anti-mouse (ThermoFisher #A28175 ...
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No products found
because this supplier's products are not listed.
Delia Onorini, et al.,
bioRxiv - Microbiology 2022
Quote:
... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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No products found
because this supplier's products are not listed.
Stefan Pan, et al.,
bioRxiv - Microbiology 2022
Quote:
... trachomatis L2 was cloned into pLATE31 (Invitrogen) to generate a C-terminal 6xHis-tagged construct ...
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No products found
because this supplier's products are not listed.
Mary R. Brockett, et al.,
bioRxiv - Microbiology 2020
Quote:
... The wild-type Chlamydia trachomatis ct276 gene was amplified by PCR with Phusion DNA polymerase (NEB) using 100 ng C ...
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No products found
because this supplier's products are not listed.
Beiyuan Liang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... After antigen retrieval using Antigen Unmasking Solution (Vector Laboratories), tissue slides were blocked with 10% Goat Serum (Vector Laboratories ...
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No products found
because this supplier's products are not listed.
Brianna Steiert, et al.,
bioRxiv - Microbiology 2022
Quote:
... and anti-Chlamydia HSP60 (Millipore Sigma). Dylight-488 and Dylight-594 (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Xiaoli Qi, et al.,
bioRxiv - Biophysics 2023
Quote:
... trachomatis Direct Specimen System (#30704, BIO-Rad) according to manufacturer’s recommendations ...
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No products found
because this supplier's products are not listed.
Anastasia-Maria Zavitsanou, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... antigen retrieval was performed using antigen retrieval buffer pH=6 (Leica) for 20min ...
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No products found
because this supplier's products are not listed.
Kok-Siong Chen, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Antigen retrieval was performed using an antigen decloaking chamber (Biocare Medical, Pacheco, California). Mounted sections were heated to 125°C for 4 minutes at 15 psi in 10 mM sodium citrate buffer (pH 6.0 ...
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No products found
because this supplier's products are not listed.
Louis Reot, et al.,
bioRxiv - Immunology 2023
Quote:
Chlamydia trachomatis serovar D (D/UW3/Cx) was obtained from Statens Serum Institut (Copenhagen, Dr Follmann laboratory). A2EN cells were seeded in EpiLife medium with EDGS ...
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No products found
because this supplier's products are not listed.
Thomas N. Burn, et al.,
bioRxiv - Immunology 2022
Quote:
... and antibodies against respective surface antigens (BD Biosciences ...
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No products found
because this supplier's products are not listed.
Neeltje van Doremalen, et al.,
bioRxiv - Microbiology 2023
Quote:
... spike S1 antigen (Sino Biological Inc. ...
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Native Antigen
Cat# CT022-1000,
1mL USD $412.0
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Scott P. Davies, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... subunit 1 (The Native Antigen Company), followed by Alexa Fluor 555-conjugated goat anti-rabbit IgG secondary antibody (Invitrogen ...
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No products found
because this supplier's products are not listed.
Vijay K. Singh, et al.,
bioRxiv - Microbiology 2022
Quote:
... After deparaffinization and antigen retrieval (Antigen Retrieval reagent; R&D Systems, Minneapolis, MN, USA), tissue sections were immersed in PBS/0.1 tween for 10 min and were blocked by Normal Goat Serum ...
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No products found
because this supplier's products are not listed.
Qidong Hu, et al.,
bioRxiv - Immunology 2022
Quote:
... Antibodies for extracellular antigens are CD3 (BioLegend 100220), CD4 (BioLegend 100540) ...
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No products found
because this supplier's products are not listed.
Travis J Gates, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and underwent antigen retrieval in antigen retrieval buffer (AR9, PerkinElmer) in a 95LC water bath ...
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No products found
because this supplier's products are not listed.
Míriam Tarrado-Castellarnau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... with antigen retrieval using Cell Conditioning 1 (CC1, Roche) for 48 minutes and primary antibody incubation for 60 minutes ...
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No products found
because this supplier's products are not listed.
Pattama Wiriyasermkul, et al.,
bioRxiv - Biochemistry 2023
Quote:
... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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No products found
because this supplier's products are not listed.
Chirag Vasavda, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and SV40T antigen (Addgene Plasmid #21826) were obtained from Addgene.
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No products found
because this supplier's products are not listed.
Emma Laporte, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Antigen retrieval in citrate buffer (Merck) was followed by permeabilization with Triton X-100 (Merck ...
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No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Antigens were unmasked by boiling the slides for 15 min in antigen unmasking citrate buffer (Cell Signaling Technology 14746), and cooled at room temperature ...
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No products found
because this supplier's products are not listed.
Valerie S. Forsyth, et al.,
bioRxiv - Microbiology 2020
Quote:
Commercially produced purified antigens were supplied by GenScript as follows ...
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No products found
because this supplier's products are not listed.
Gaël Moquin-Beaudry, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... SV40 LargeT antigen (Santa Cruz, clone Pab 101), and GD2 (Biolegend ...
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No products found
because this supplier's products are not listed.
Lauren E. Stopfer, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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No products found
because this supplier's products are not listed.
Noah Mishkin, et al.,
bioRxiv - Microbiology 2022
Quote:
... the primary antibody against Chlamydia MOMP (NB100-65054, Novus Biologicals, Centennial, CO) was applied at a dilution of 1:500 ...
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No products found
because this supplier's products are not listed.
Linling He, et al.,
bioRxiv - Microbiology 2019
Quote:
... Each immunization consisted of 200 μl of antigen/adjuvant mix containing 50 μg of vaccine antigen and 100 μl of AddaVax adjuvant (Invivogen) via the subcutaneous (s.c. ...
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No products found
because this supplier's products are not listed.
Katherine Minjee Chung, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... in a 2100 Antigen Retriever system (Electron Microscopy Sciences). Sections were then incubated with dual endogenous peroxidase block (Dako ...
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No products found
because this supplier's products are not listed.
Amber Barton, et al.,
bioRxiv - Immunology 2023
Quote:
... trachomatis LPS (Abnova) in PBS followed by incubation with goat-anti-mouse IgG conjugated with AlexaFluor 647 ...
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No products found
because this supplier's products are not listed.
Rafaela Schober, et al.,
bioRxiv - Immunology 2022
Quote:
... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
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No products found
because this supplier's products are not listed.
Andrew Sawyer, et al.,
bioRxiv - Immunology 2022
Quote:
... Antigen retrieval was performed by boiling samples to 100°C for 15 minutes in pH 9 Antigen retrieval buffer (Akoya). Tissues were incubated for 45 minutes at room temperature with primary antibodies specific for CD20 (L26 ...
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No products found
because this supplier's products are not listed.
Elaine Thai, et al.,
bioRxiv - Immunology 2020
Quote:
Antigen ELISA detHigh binding 384 well polystyrene plates (Corning) were coated with recombinantly expressed PfCSP71-104 comprising N-CSP at 50 ng/well overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Frederic Bibollet-Ruche, et al.,
bioRxiv - Immunology 2022
Quote:
... Supernatants were assayed for p27 antigen production by ELISA (Zeptometrix).
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No products found
because this supplier's products are not listed.
Alyssa N Coyne, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Antigen retrieval was performed using Tissue-Tek antigen retrieval solution (IHC World) for 1 hour in a steamer ...
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No products found
because this supplier's products are not listed.
Kim S. Friedmann, et al.,
bioRxiv - Immunology 2020
Quote:
... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
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No products found
because this supplier's products are not listed.
Zeqing Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Antigen retrieval was performed with Antigen Retrieval Solution (Solarbio, C1035) before the conventional immunostaining procedure.
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No products found
because this supplier's products are not listed.
Andrea R. Shiakolas, et al.,
bioRxiv - Immunology 2021
Quote:
... AviTagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
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No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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No products found
because this supplier's products are not listed.
Manutsanun Inthawong, et al.,
bioRxiv - Immunology 2022
Quote:
... A T cell antigen control pool (CEF, Mabtech AB, 3616-1) at a final concentration of 1 μg/ml was used as a source of control antigens ...
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No products found
because this supplier's products are not listed.
Miguel A. B. Mercado, et al.,
bioRxiv - Immunology 2023
Quote:
... Chlamydia-specific Abs were detected using HRP-based SBA Clonotyping System (Southern Biotech).
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No products found
because this supplier's products are not listed.
Jesse M. Hall, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antigen specific antibody titers to PT (List Biological Laboratories #180) were measured by coating ELISA plates with 50 μl of antigen per well ...
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No products found
because this supplier's products are not listed.
Nuno Apóstolo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1:50 donkey anti-mouse IgG antigen-binding fragments (Jackson ImmunoResearch). Sections were then washed in PBS-0.5%Triton X-100 at RT and incubated at 4°C overnight with primary antibodies in PBS containing 5% NHS ...
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No products found
because this supplier's products are not listed.
Jianlong Zhai, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the antigen-antibody complexes were detected using Odyssey XF (LI-COR, USA). The signal intensities of target bands were analyzed using ImageJ software (National Institutes of Health ...
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No products found
because this supplier's products are not listed.
Jerry Z. Xu, et al.,
bioRxiv - Microbiology 2019
Quote:
... trachomatis-infected WT and TLR3-deficient epithelial cells using the RNeasy kit plus (Qiagen, Valencia, CA). The DNA-free RNA was quantified using the NanoDrop spectrophotometer (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Eishani Kumar Sokolowski, et al.,
bioRxiv - Genomics 2024
Quote:
... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 100 ng of antigen was coated on 96 well microtiter plates (High binding, Greiner) in PBS (pH 7.4 ...
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No products found
because this supplier's products are not listed.
Paul Castillo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Synthetic mRNA encoding for full-length model antigen (i.e., CleanCap GFP, catalog L-7601 and CleanCap ovalbumin, OVA, catalog L-7610) were purchased from TriLink Biotechnologies (San Diego ...
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No products found
because this supplier's products are not listed.
Stefanie Koster, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mouse-anti-Chlamydia Hsp60 (A57-E4) (1:500, Enzo Life Sciences, # ALX-804-071-R100 and 1:1000 GeneTex ...
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No products found
because this supplier's products are not listed.
Teresa R. Wagner, et al.,
bioRxiv - Immunology 2023
Quote:
... purified antigen (Acrobiosystems) was reacted with Sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Stefanie Koster, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mouse-anti-Chlamydia Hsp60 (A57-E4) (1:500, Enzo Life Sciences, # ALX-804-071-R100 and 1:1000 GeneTex, # GTX25486) and goat-anti-Chlamydia major outer membrane protein (MOMP ...
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No products found
because this supplier's products are not listed.
Caroline E. Dewar, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The antibody was affinity purified against the antigen by Eurogentec, and used at a 1:50 dilution ...