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No products found
because this supplier's products are not listed.
Riki Kawamura, Masato Nikaido,
bioRxiv - Neuroscience 2022
Quote:
... dehydroepiandrosterone 3-sulfate (DHEA-s), β-estradiol 17-(β-D-glucuronide) (E2-17g), and β-estradiol 3,17-disulfate (E2-3, 17s) were respectively purchased from Tokyo Chemical Industry, Cayman Chemical Co. ...
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No products found
because this supplier's products are not listed.
Christine Vazquez, Chin Yee Tan, Stacy M. Horner,
bioRxiv - Microbiology 2019
Quote:
... and rabbit anti-Sendai virus (SV) (1:1000, MBL International). Secondary antibody incubations were done with Alexa Fluor conjugated antibodies (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Keith R. Carney, et al.,
bioRxiv - Developmental Biology 2019
Quote:
Embryos were dechorionated at 24 hpf and embedded in 1.6% low melting point agarose (in E2+gentamycin) in Delta T dishes (Bioptechs (#0420041500C)) ...
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No products found
because this supplier's products are not listed.
Dennis M Defoe, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... then incubated overnight at 4°C in combined primary antibodies (chicken anti-green fluorescent protein [GFP]; Aves Labs, Tigard ...
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No products found
because this supplier's products are not listed.
Marie S. Prevost, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The supernatant containing solubilized proteins was bound overnight at 4°C on a gravity flow Rho1D4 resin (Cube Biotech) equilibrated with buffer A supplemented with 0.05% DDM ...
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No products found
because this supplier's products are not listed.
Chitra Latka, et al.,
bioRxiv - Biophysics 2020
Quote:
... 2 M ammonium sulfate and 2% PEG 400 at 24 °C and confirmed as protein crystals by addition of Izit dye (Hampton Research). In order to obtain crystals reproducibly ...
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No products found
because this supplier's products are not listed.
V D’Arienzo, et al.,
bioRxiv - Microbiology 2020
Quote:
... cDNA synthesis was performed with 0.25-1 µg of RNA in a 20 µL total reaction volume using a random hexamer/oligo dT strand synthesis kit as per the manufacturer’s instructions (10 min at 25°C; 15 min at 42°C; 15 min at 48°C; SensiFast, Bioline). All oligonucleotide sequences are listed in Table 1.
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No products found
because this supplier's products are not listed.
Gyu Rie Lee, et al.,
bioRxiv - Biochemistry 2023
Quote:
... anti-c-Myc fluorescein isothiocyanate (anti-c-Myc-FITC, Immunology Consultants Laboratory) and streptavidin-phycoerythrin (SAPE ...
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No products found
because this supplier's products are not listed.
Florian J. Bock, et al.,
bioRxiv - Cell Biology 2020
Quote:
... S63845 (Chemgood, C-1370), Sytox Green (Thermo ...
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No products found
because this supplier's products are not listed.
Yuan Xue, Ido Braslavsky, Stephen R. Quake,
bioRxiv - Biochemistry 2021
Quote:
Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Nadezda A. Brazhe, et al.,
bioRxiv - Biophysics 2022
Quote:
... The temperature of the coverslips was maintained at 340 °C (magnetic stirrer with heating IKA C-MAG HS 4 digital) during the experiment and 15 min after ...
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No products found
because this supplier's products are not listed.
Zhang Feng, Omar E. Alvarenga, Alessio Accardi,
bioRxiv - Biochemistry 2024
Quote:
... and resuspended in buffer C and 40 mM sodium cholate (Anatrace) at a final lipid concentration of 20 mM ...
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No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
A modified protocol using a commercially available human anti-zika virus Envelope protein (ZIKV-Env) IgG kit (Alpha Diagnostics International) was used to quantify ZIKV-117 mAb levels in NHP serum samples ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
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No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
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No products found
because this supplier's products are not listed.
Liriye Kurtovic, et al.,
bioRxiv - Immunology 2020
Quote:
... A volume of 50 μL of the CSP-dS VLP preparation was applied to TSKgel® G6000PWXL 7.8*300 mm column (Tosoh Bioscience GmbH, Griesheim, Germany) in loading buffer (1.7 mM KH2PO4 ...
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No products found
because this supplier's products are not listed.
Georges Raad, et al.,
bioRxiv - Genetics 2020
Quote:
... adiponectin and cholesterol levels were measured with the C-Reactive Protein ELISA (Mouse CRP, Elabscience, CliniSciences S.A.S. ...
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No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
... A fixed concentration of SARS-CoV-2 GFP pseudotyped virus (BPS Biosciences) was added and the plate incubated for 60 minutes at 37°C and 5% CO2 ...
-
WB, IF, IHC,ELISA
Cat# A5006, SKU# A5006-100ul,
100ul, $157.00
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Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
-
No products found
because this supplier's products are not listed.
Juan F. Codocedo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... One portion of the homogenate was further sonicated for protein extraction and western blot and ELISA analysis and the other was stored at -80 °C in RNA bee (Amsbio, CS-501B) for RNA extraction and gene expression analysis.
-
No products found
because this supplier's products are not listed.
Han Kang Tee, et al.,
bioRxiv - Microbiology 2019
Quote:
... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
-
No products found
because this supplier's products are not listed.
Pin-Ji Lei, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... we inoculated 1×105 PFU UV-inactivated PR8 influenza virus + 2μg Anti-CD40 (BioXCell, Cat: BE0016-2) + 2μg poly I:C (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Gloria Asantewaa, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 75 µg of protein lysates were heated for 10 mins at 95°C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml ...
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No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
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No products found
because this supplier's products are not listed.
Bibiana Rius, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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No products found
because this supplier's products are not listed.
Brunno R. Levone, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Proteins were quantified using a validated BCA (Bicinchoninic Protein Assay Kit, EuroClone) protocol ...
-
No products found
because this supplier's products are not listed.
Ourania Galanopoulou, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
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No products found
because this supplier's products are not listed.
Flavia Ferrantelli, et al.,
bioRxiv - Microbiology 2022
Quote:
... virus dilutions and number of deaths/group after challenge were used to calculate the LD50 (Quest Graph™ LD50 Calculator, AAT Bioquest, Inc.). The Kaplan-Meier curve was used to show survival rate differences among groups of animals challenged with different doses of SARS-CoV-2 virus ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... or C1s proteins (Complement Technologies), or BSA (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Sharon Tran, et al.,
bioRxiv - Cell Biology 2023
Quote:
... by their overnight incubation at 56°C followed by 1 hour at 85°C in DirectPCR (Tail) Lysis Reagent (Viagen) supplemented with 0.2 % (v/v ...
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No products found
because this supplier's products are not listed.
Isadora Matias, et al.,
bioRxiv - Neuroscience 2021
Quote:
Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore ...
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No products found
because this supplier's products are not listed.
Lisa-Marie Appel, et al.,
bioRxiv - Biochemistry 2022
Quote:
Crystalization was performed at at 22°C or 4°C using a sitting-drop vapour diffusion technique and micro-dispensing liquid handling robot Mosquito (TTP labtech). The best diffracting crystals of SHARP SPOC:1xS5P CTD were grown at 22°C in conditions E9 from ShotGun HT screen (SG1 HT96 Molecular Dimensions ...
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No products found
because this supplier's products are not listed.
Muhammad Rizwan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Recombinant protein G (Cedarlane Labs Inc). All solvents were purchased from Caledon ...
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No products found
because this supplier's products are not listed.
Lingling Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... feeder cells were treated with Mitomycin C (Apexbio Technology) at the concentration of 10 μg/mL for 2 hours and then washed with PBS ...
-
No products found
because this supplier's products are not listed.
Krzysztof Kucharz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... at 70°C using a mini-extruder (Avanti Polar Lipids) to form nanoparticles.
-
No products found
because this supplier's products are not listed.
Ahmed E.I. Hamouda, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 37°C and a Cascade 512B EMCCD camera (Photometrics, USA) operated with IPlab Spectrum software (Scanalytix ...
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No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
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No products found
because this supplier's products are not listed.
Teresa Attenborough, et al.,
bioRxiv - Genomics 2024
Quote:
... incubating overnight at 4°C in DAPI fluoromount-G (Southern Biotech) and then mounting and imaging on a confocal laser microscope (Leica Sp8).
-
With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
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Ethan L. Morgan, et al.,
bioRxiv - Microbiology 2020
Quote:
... Proteins were detected using WesternBright ECL (Advansta, USA) and visualised on X-ray film.
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No products found
because this supplier's products are not listed.
Kevin W. Bollinger, et al.,
bioRxiv - Microbiology 2024
Quote:
... and Ldt3 was raised against purified protein (ProSci). To analyze protein levels by Western immunoblotting ...
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No products found
because this supplier's products are not listed.
Paul D. Simonson, Itzel Valencia, Sanjay S. Patel,
bioRxiv - Immunology 2022
Quote:
... The standard Akoya CODEX protocols were followed (Akoya CODEX manual, revision C). For tyramide-barcode staining ...
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No products found
because this supplier's products are not listed.
Komal Soni, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... was homogenized at 4°C using 700 µL zirconia beads (BioSpec, 110791) in Precellys 24 homogenizer (Bertin Technologies ...
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No products found
because this supplier's products are not listed.
Annemarie Lang, et al.,
bioRxiv - Bioengineering 2024
Quote:
... KS) and moved onto a heating pad (37 °C; Kent Scientific, Torrington, CT). Anesthesia was maintained at ∼1.5–2% with an individual a nose cone ...
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No products found
because this supplier's products are not listed.
Kartikeya Vijayasimha, et al.,
bioRxiv - Immunology 2022
Quote:
... The ubiquitin E1 inhibitor MLN7243 (ChemieTek), and NEDD8 E1 inhibitor MLN4924 (Millipore ...
-
No products found
because this supplier's products are not listed.
Ida Søgaard Larsen, et al.,
bioRxiv - Microbiology 2023
Quote:
... and C-peptide by Mouse C-peptide ELISA kit (Crystal Chem) following the manufacturers’ protocols.
-
No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/ml of cytosine arabinoside (TriLink BioTechnologies), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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No products found
because this supplier's products are not listed.
Huacheng Luo, et al.,
bioRxiv - Genetics 2019
Quote:
... followed by drying at 37°C and Giemsa staining (Amresco)28.
-
No products found
because this supplier's products are not listed.
Arnaud Rondelet, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and derivatives were grown at 5% CO2 and 37 °C in DMEM (PAN Biotech) supplemented with 10% filtered Fetal Bovine Serum (FBS ...