-
No products found
because this supplier's products are not listed.
Carolyn M. Highland, et al.,
bioRxiv - Biochemistry 2022
Quote:
HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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No products found
because this supplier's products are not listed.
Margarita V. Rangel, et al.,
bioRxiv - Microbiology 2021
Quote:
... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
-
No products found
because this supplier's products are not listed.
Gui-Zhen Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 0.5 μg E1 (abcam), 2.5 μg UbcH5a/UBE2D1 (Boston Biochem) ...
-
No products found
because this supplier's products are not listed.
Tino Pleiner, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 100 nM GST-tagged Ubiquitin-activating enzyme E1 and 250 nM Ubiquitin-conjugating enzyme E2 UbcH5 [both from Boston Biochem, USA]) ...
-
No products found
because this supplier's products are not listed.
Zanetta Kechagia, et al.,
bioRxiv - Cell Biology 2022
Quote:
... mouse anti-Lamin A/C (E1) 1:100 (Santa Cruz, Cat# sc376248), rabbit anti-Tri-Methyl-Histone H3 (Lys27 ...
-
No products found
because this supplier's products are not listed.
Barbara Clough, et al.,
bioRxiv - Immunology 2023
Quote:
The E1 inhibitor PYR41 (Tocris #2978) was added to cells prior to infection at a concentration of 50μM and incubated at 37°C for 2h ...
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No products found
because this supplier's products are not listed.
Rachel Grazda, et al.,
bioRxiv - Immunology 2023
Quote:
Resolvin E1 (250ng; Cayman Chemical) was diluted in PBS and administered to mice via intraperitoneal injection on days 7 ...
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No products found
because this supplier's products are not listed.
Apurva T. Prabhakar, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... HA) (E1), HPV16 E2, pGL3 Basic, pGL3 Control, ptk6E2 (22, 68, 69) E2-K mutant plasmids were generated by GenScript.
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No products found
because this supplier's products are not listed.
Yang Yang, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 0.2 mM E2 conjugase (UbcH5c, #E2-627 R&D systems, or RAD6 #E2-613 R&D Systems), 0.2 mM E3 ligase (recombinant bacterial RAD18-RAD6 complex or RNF168 both purified in-house) ...
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No products found
because this supplier's products are not listed.
Daniel Loftus, et al.,
bioRxiv - Molecular Biology 2023
Quote:
dCas9-VPR and Cas9 experiments were performed using a published multiplex four sgRNA construct system.79 The four sgRNAs for each of the sgRNA target sites (Kcnk9 TSS, E1, and E2) were cloned into four sgRNA backbones each containing different small RNA promoters (Addgene #53186, #53187, #53188, #53189). Golden Gate cloning into pLV-GG-hUbC-dsRED (Addgene #84034 ...
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No products found
because this supplier's products are not listed.
Diana P. Baquero, et al.,
bioRxiv - Microbiology 2020
Quote:
... the VLPs were concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor), resuspended in buffer A and observed by TEM ...
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No products found
because this supplier's products are not listed.
Shirley Bikel, et al.,
bioRxiv - Genomics 2020
Quote:
The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
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No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus NS4B protein (Genetex, GTX124250) at 1:1000 ...
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No products found
because this supplier's products are not listed.
Julia S. Spear, Katharine A. White,
bioRxiv - Cell Biology 2023
Quote:
... α-cyclin E1 (1:1000; Cell Signaling, 4129), actin (1:1,000 ...
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No products found
because this supplier's products are not listed.
Vedita Anand Singh, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... is inserted in n-terminal of E2 protein of CHIKV 181/25 structural genes (C-E3-E2-6K-E1) and cloned into pcDNA3.1(+) expression vector using gibson assembly cloning kit (NEB, USA). The resulting plasmid is designated as ChAC-VLP ...
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No products found
because this supplier's products are not listed.
Daniel Thoresen, et al.,
bioRxiv - Microbiology 2023
Quote:
... Total protein concentration of purified DENV VLP was measured by Quick Start Bradford Protein Assay (Bio-Rad). Purity of the DENV VLP was assessed by SDS-PAGE followed by Coomassie dye-based staining using QC colloidal Coomassie stain (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Emily Delgouffe, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... and E2 (E170 Modular, Roche, Gen III ...
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No products found
because this supplier's products are not listed.
Anabel Guedán, et al.,
bioRxiv - Microbiology 2021
Quote:
... VLP were treated with 20 units/mL RQ1-DNase (Promega) in 10mM MgCl2 for 1h at 37°C before infection ...
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No products found
because this supplier's products are not listed.
Janina Rahlff, et al.,
bioRxiv - Microbiology 2023
Quote:
... Prokaryotic cells and virus-like particles (VLPs) from site #1 – #3 were measured on a flow cytometer (BD Accuri C6 ...
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No products found
because this supplier's products are not listed.
Natalia Vydra, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 17β-estradiol (E2; Merck KGaA) was added 48 hours later to a final concentration of 10 nM (an equal volume of ethanol was added as vehicle control ...
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No products found
because this supplier's products are not listed.
Jakub Jankowski, et al.,
bioRxiv - Genetics 2024
Quote:
CRISPR-Cas9 targeted mice were generated using B6D2F1/J (deletion of enhancer 1, E1) or C57BL/6N (E2) (Charles River) or by the transgenic core of the National Heart ...
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No products found
because this supplier's products are not listed.
D. Puigoriol-Illamola, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The DNA sequence for E2-Crimson was sourced from Clontech. The gene for the fluorescent-HSD11B1 was ligated into vector pcDNA3.1 (Invitrogen ...
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No products found
because this supplier's products are not listed.
Ayat Zawawi, et al.,
bioRxiv - Immunology 2019
Quote:
... The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6, 10/300 GL;GE Healthcare). The level of endotoxin in all the purified VLPs was measured with an ELISA-based endotoxin detection assay (Hyglos ...
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No products found
because this supplier's products are not listed.
N Ogino, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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No products found
because this supplier's products are not listed.
Laura Schenkel, et al.,
bioRxiv - Cell Biology 2023
Quote:
... either at the Visitron Spinning Disk (experiments e1 (internal Lab ID: EXP345) and e2 (internal Lab ID: EXP337)) or a Nikon Wide Field microscope (Nikon Ti2-E (inverse), experiments e3 and e4 (internal Lab ID ...
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No products found
because this supplier's products are not listed.
Yunlong Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... G*ΔG-VSV virus (VSV G pseudotyped virus, Kerafast) is used to infect 293T cells (American Type Culture Collection [ATCC] ...
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No products found
because this supplier's products are not listed.
Lee Sherry, et al.,
bioRxiv - Microbiology 2022
Quote:
... quantified PV VLPs were diluted in phosphate buffered saline (Corning 46-013-CM) to provide a uniform quantity of D antigen ...
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No products found
because this supplier's products are not listed.
Sophie L. Winter, Petr Chlanda,
bioRxiv - Biophysics 2021
Quote:
VLPs were plunge frozen in liquid ethane kept at - 183°C using GP2 (Leica). A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion ...
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No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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No products found
because this supplier's products are not listed.
Shivani N. Mann, et al.,
bioRxiv - Physiology 2020
Quote:
... 17α-E2 powder (Steraloids, Newport, RI) was dissolved in DMSO at a concentration of 10mg/ml and stored at −80°C ...
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No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
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No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
-
No products found
because this supplier's products are not listed.
Daniel Perez-Zsolt, et al.,
bioRxiv - Microbiology 2021
Quote:
... Virus particles were immunostained with rabbit anti-SARS-CoV-2 N protein (Sino Biological) or rabbit anti-GM1 Ab (Abcam ...
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No products found
because this supplier's products are not listed.
Joseph M Gibbons, et al.,
bioRxiv - Microbiology 2019
Quote:
... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
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No products found
because this supplier's products are not listed.
Jean-Philippe Guégan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... E2 UbcH5c (2.5 µM, Enzo Life Sciences), biotinylated Ubiquitin (2.5 µM ...
-
No products found
because this supplier's products are not listed.
Thao Thu Mai, et al.,
bioRxiv - Immunology 2021
Quote:
... The culture supernatant containing the virus was centrifuged at 4,500 g for 5 min at 4°C (Eppendorf 5810R) and stored at -80°C ...
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No products found
because this supplier's products are not listed.
A Goikoetxea, et al.,
bioRxiv - Physiology 2021
Quote:
... whereas label for the E2 assay was acquired from Perkin Elmer. Antiserum against E2 was purchased from MyBioSource and antiserum against 11KT was kindly donated by Emeritus Professor Yoshitaka Nagahama ...
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No products found
because this supplier's products are not listed.
Lucy Gordon, et al.,
bioRxiv - Microbiology 2021
Quote:
... A 5 μg/ml concentration of biotinylated stable O1/Manisa/TUR/69 FMD VLP (61) (kindly provided by Alison Burman) was immobilised on streptavidin-coated biosensors (Sartorius UK Limited) for 900 s ...
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No products found
because this supplier's products are not listed.
Angie Molina, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Electroporated E2 embryos were collected in PBS and 100 μm slices were obtained using a McIlwain tissue chopper (WPI), from the brachial region corresponding to somites 12 to 17 which generates the greatest number of motoneurons (Oppenheim ...
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No products found
because this supplier's products are not listed.
Enrique Lin-Shiao, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Concentrated VLPs were resuspended in SE buffer (Lonza) and electroporated with 2-4pmol HDR template using a 4-D Nucleofector with pulse code CM-150 (Lonza) ...
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No products found
because this supplier's products are not listed.
Carina Sandoval, Karly Nisson, Oliver I. Fregoso,
bioRxiv - Microbiology 2024
Quote:
... Virus-like particles (VLPs) packaging Vpr WT or mutant proteins were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). VLPs were harvested 48 hrs post transfection ...
-
No products found
because this supplier's products are not listed.
Shao-Jung Hsu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... one given adeno-associated virus (AAV)8-mouseVEGF-C (Vector Biolabs, Malvern, PA) and the other given AAV8-GFP (control ...
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No products found
because this supplier's products are not listed.
Jared R. Mayers, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli E1 strain was grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
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No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Zhaoqi Li, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-Pyruvate Dehydrogenase E1-alpha subunit (total) (Proteintech, 18068-1-AP), MPC2 (Cell Signaling Technologies ...
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Recombinant Antigen
Cat# CHIKV-VLP-100,
100µg USD $773.0
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Carmen Mirabelli, et al.,
bioRxiv - Microbiology 2021
Quote:
... HNoV GII.4 virus-like particles (VLPs) were purchased from The Native Antigen Company, Poly (I:C ...
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No products found
because this supplier's products are not listed.
Keith A. Breau, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and 10 nM Prostaglandin E2 (Peprotech 3632464). For plating of primary crypts and following passaging ...
-
No products found
because this supplier's products are not listed.
Paulo V. M. Boratto, et al.,
bioRxiv - Microbiology 2020
Quote:
... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...