-
No products found
because this supplier's products are not listed.
Ferdinando M. Milazzo, et al.,
bioRxiv - Microbiology 2022
Quote:
... 1.0 μg/mL human ACE2 protein mouse Fc-tag (Sino Biological) was added and incubated for 1 h at 37 °C ...
-
No products found
because this supplier's products are not listed.
Malcolm J. W. Sim, et al.,
bioRxiv - Immunology 2019
Quote:
... The PresentER plasmid encoding a signal peptide from Mouse Mammary Tumor Virus envelope protein followed by the SIINFEKL epitope followed by mCherry was obtained from Addgene (#102945),a kind gift of D ...
-
No products found
because this supplier's products are not listed.
Mayank Verma, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... were incubated with mouse FLT1-FC chimeric protein with His-tag (471-F1-100, R&D Systems). Plates were washed with 0.05% Tween 20/PBS ...
-
No products found
because this supplier's products are not listed.
Mikail Dogan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
-
No products found
because this supplier's products are not listed.
Apurva T. Prabhakar, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... E2 tag [5E11] 1:1000 (Abcam, catalog no. ab977).
-
No products found
because this supplier's products are not listed.
Silvia Kocanova, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were grown for 3 days in phenol red-free media supplemented with 10% charcoal-stripped FCS (-E2) and subsequently treated with 100nM E2 (Sigma) for the indicated times.
-
No products found
because this supplier's products are not listed.
N Ogino, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
-
No products found
because this supplier's products are not listed.
Fang Tian, et al.,
bioRxiv - Biophysics 2021
Quote:
... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
-
No products found
because this supplier's products are not listed.
Katherine E. Uyhazi, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Cyclin E2 polyclonal rabbit anti-mouse (Cell Signaling), Suz 12 polyclonal rabbit anti-mouse (Cell Signaling) ...
-
No products found
because this supplier's products are not listed.
Mona O. Mohsen, et al.,
bioRxiv - Immunology 2021
Quote:
... anti-mouse CD16/CD32 (mouse BD Fc block) mAb clone 2.4G2 (BD Bioscience ...
-
No products found
because this supplier's products are not listed.
Xin Ee Yong, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... vector containing DENV2C protein gene sequence with N-terminal His tag and Tobacco Etch Virus (TEV) digestion site was purchased from GenScript (China). Recombinant capsid protein from DENV2 NGC strain was expressed in Escherichia coli BL21 strain ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Prostaglandin E2 10nM (Tocris) was added only to medium for normal tissue organoids.
-
No products found
because this supplier's products are not listed.
Marco Brustolin, et al.,
bioRxiv - Microbiology 2021
Quote:
... Then cells were labeled with the monoclonal anti-CHIKV E2 envelope glycoprotein clone CHK-48 made in mouse (BEI Resources, Manassas, VA, USA) and the polyclonal anti-Zika virus envelope protein made in rabbit (GeneTex, Irvine, CA, USA). The antibodies used for immunofluorescence included Alexa Fluor 488 conjugated goat anti-rabbit IgG (Invitrogen ...
-
No products found
because this supplier's products are not listed.
June-Hyung Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... and 1 µg of mouse E2 (UBE2E3, Boston Biochem) in 40 µl of reaction buffer (Boston Biochem ...
-
No products found
because this supplier's products are not listed.
Chanchal Chauhan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Virus containing supernatants were harvested five times from transfected Ecopack-HEK293 cells (Clontech/Takara), over a period of three days post-transfection and filtered with 0.45 µm filters ...
-
No products found
because this supplier's products are not listed.
Toufic Kassouf, et al.,
bioRxiv - Cell Biology 2023
Quote:
... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
-
No products found
because this supplier's products are not listed.
Yun-Fei Ma, et al.,
bioRxiv - Immunology 2024
Quote:
... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
-
No products found
because this supplier's products are not listed.
Lei Qiang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... GAPDH and HA-Tag (mouse) (Santa Cruz), E-cadherin ...
-
No products found
because this supplier's products are not listed.
Ayijiang Yisimayi, et al.,
bioRxiv - Immunology 2023
Quote:
Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
-
No products found
because this supplier's products are not listed.
Hangu Nam, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Fc Tag (ACROBiosystems). To obtain comparable live cell counts between conditions ...
-
No products found
because this supplier's products are not listed.
Conor W. Mitchell, et al.,
bioRxiv - Biochemistry 2023
Quote:
... mouse α-His-tag (1:1,000 ; Proteintech), α-O-GlcNAc (RL2 ...
-
No products found
because this supplier's products are not listed.
Amy Lewis, Philip Michael Elks,
bioRxiv - Immunology 2019
Quote:
... Exogenous prostaglandin E2 (Cayman Chemicals) and 15-keto prostaglandin E2 (Cayman Chemicals ...
-
No products found
because this supplier's products are not listed.
Nathalie Bastié, et al.,
bioRxiv - Molecular Biology 2023
Quote:
- Mouse anti-V5 tag (Bio-Rad, MCA1360) used at dilution 1:5000 for Western Blot
-
No products found
because this supplier's products are not listed.
Déborah Lécuyer, et al.,
bioRxiv - Microbiology 2023
Quote:
... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
-
No products found
because this supplier's products are not listed.
Natalia Vydra, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 17β-estradiol (E2; Merck KGaA) was added 48 hours later to a final concentration of 10 nM (an equal volume of ethanol was added as vehicle control ...
-
No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Rebecca S. Brown, et al.,
bioRxiv - Microbiology 2020
Quote:
... E2 signal was imaged and quantified using the Odyssey Fc Imaging System (LI-COR Biosciences) to determine particle number ...
-
No products found
because this supplier's products are not listed.
Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... DSG2 and DSG3-Fc proteins and N-CAD-Fc protein were detected with the following antibodies: mouse-anti-DSG2 (#BM5016, Origene, 1:200); mouse-anti-DSG3 5G11 (#32-6300 ...
-
No products found
because this supplier's products are not listed.
Renée M. van der Sluis, et al.,
bioRxiv - Immunology 2021
Quote:
... the SARS-CoV-2 envelope (E) protein (ABclonal RP01263) or the SARS-CoV-2 spike (S ...
-
No products found
because this supplier's products are not listed.
Annette B. Vogel, et al.,
bioRxiv - Immunology 2020
Quote:
... Anti-mouse-Fc-antibody (Jackson ImmunoResearch) was diluted in 10 mM sodium acetate buffer pH 5 (30 µg/mL ...
-
No products found
because this supplier's products are not listed.
Vidur Garg, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Coding sequences for mouse Gata4 and E2-Crimson preceded by an E2A self-cleaving peptide (E2A-E2-Crimson) were assembled using NEBuilder HiFi DNA assembly (NEB) to generate pCX-Gata4-E2A-E2-Crimson ...
-
No products found
because this supplier's products are not listed.
Nikhil Khatwani, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-mouse FC blocking Ab (Bioxcell) was applied on ice for 15 minutes to samples prepared as described above ...
-
No products found
because this supplier's products are not listed.
Tanne van der Wal, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
-
No products found
because this supplier's products are not listed.
Danny Simpson, et al.,
bioRxiv - Genomics 2023
Quote:
... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
-
No products found
because this supplier's products are not listed.
Sofya A. Polyanskaya, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
-
No products found
because this supplier's products are not listed.
Shingo Hanaoka, Shinji Saijou, Yasuhiro Matsumura,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... reacted with HRP-conjugated anti-human IgG-Fc antibody or anti-mouse IgG-Fc antibody (Bethyl) for 1 hour ...
-
No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
Cellular immunofluorescence for nuclear envelope proteins was performed using 1205Lu cells plated on cleaned coverslips (#1.5, 22 mm x 22 mm, Corning) for 24-72 hours depending on experimental conditions ...
-
No products found
because this supplier's products are not listed.
M. Zeeshan Chaudhry, et al.,
bioRxiv - Microbiology 2021
Quote:
... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
-
No products found
because this supplier's products are not listed.
Peng He, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... cDNA libraries were then tagmented using the Illumina/Nextera DNA prep kit (FC 121-1030) with index tags from Illumina (FC 121-1031), cleaned up with Ampure XP beads ...
-
No products found
because this supplier's products are not listed.
Vineet Choudhary, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
-
No products found
because this supplier's products are not listed.
Laurent Gros, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Kyung-Tae Lee, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
No products found
because this supplier's products are not listed.
Alexander P. Horkowitz, et al.,
bioRxiv - Immunology 2020
Quote:
... Mice were infected with the mouse adapted influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) (Charles River Laboratories Avian Vaccine Services ...
-
No products found
because this supplier's products are not listed.
Javed Akhter, et al.,
bioRxiv - Microbiology 2020
Quote:
... The recombinant Envelope protein was obtained from MyBioSource (Cat#MBS8309649). All other reagents were from Sigma Aldrich.
-
No products found
because this supplier's products are not listed.
Shivani N. Mann, et al.,
bioRxiv - Physiology 2020
Quote:
... 17α-E2 powder (Steraloids, Newport, RI) was dissolved in DMSO at a concentration of 10mg/ml and stored at −80°C ...
-
No products found
because this supplier's products are not listed.
Keith A. Breau, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... and 10 nM Prostaglandin E2 (Peprotech 3632464). For plating of primary crypts and following passaging ...
-
No products found
because this supplier's products are not listed.
A Goikoetxea, et al.,
bioRxiv - Physiology 2021
Quote:
... whereas label for the E2 assay was acquired from Perkin Elmer. Antiserum against E2 was purchased from MyBioSource and antiserum against 11KT was kindly donated by Emeritus Professor Yoshitaka Nagahama ...
-
No products found
because this supplier's products are not listed.
Mateusz Pękacz, et al.,
bioRxiv - Molecular Biology 2023
Quote:
The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...