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No products found
because this supplier's products are not listed.
Anne Tersteegen, et al.,
bioRxiv - Immunology 2021
Quote:
Total DNA was isolated from tissues using a DNA isolation kit (Genekam) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Vasiliy S. Chernyshev, et al.,
bioRxiv - Bioengineering 2021
Quote:
The EV isolation followed the protocol provided by the column manufacturer (PURE-EVs, HansaBioMed, Estonia). Briefly ...
-
No products found
because this supplier's products are not listed.
Monika Rakoczy-Trojanowska, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The protein-antibody complex was detected with the ECL Superbright system (Agrisera) and photographed using the ChemiDoc Imaging System (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Katie E. Smith, et al.,
bioRxiv - Physiology 2019
Quote:
... before sectioning at 150 µm intervals on a vibratome (1000 plus system, Intracel). Tissues were not decalcified prior to sectioning ...
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No products found
Girish Nallur,
bioRxiv - Molecular Biology 2023
Quote:
Cells and reagents: DOHH2 cells were purchased from Creative Biomart. Cells were grown at 5% CO2 in 90% RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
L. Robert Hollingsworth, et al.,
bioRxiv - Immunology 2020
Quote:
... The chromatography system was coupled to a three-angle light scattering detector (mini-DAWN TRISTAR) and a refractive index detector (Optilab DSP ...
-
No products found
because this supplier's products are not listed.
Amélie Fréal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... AnkG (Life technologies #33-8800, Neuromab/Antibodies Incorporated #75-146 and Synaptic systems #386-005), βIV-spectrin (Biotrend ...
-
No products found
because this supplier's products are not listed.
Titouan Bonnot, et al.,
bioRxiv - Plant Biology 2024
Quote:
... samples were filtered through a 0.45 μm teflon filtration system (Digifilter, SCP Science, Courtaboeuf, France). Quantification of each element was performed using external standard calibration curves and concentrations were expressed in μg.g-1 of leaves.
-
No products found
because this supplier's products are not listed.
Jakub Gemperle, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The system was recalibrated for the refractive index of the 1x Opti-Klear medium (Marker Gene Technologies Inc) supplemented with 10% (v/v ...
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No products found
Jesse M. Hall, et al.,
bioRxiv - Immunology 2021
Quote:
... the lung homogenate was pushed through a 70 µm cell strainer (BioDesign Cell MicroSives Cat. N70R), creating a single-cell suspension ...
-
No products found
because this supplier's products are not listed.
Daniel A Skelly, et al.,
bioRxiv - Genomics 2019
Quote:
Cell lines were genotyped by Neogen Corp ...
-
No products found
because this supplier's products are not listed.
Sebald A. N. Verkuijl, et al.,
bioRxiv - Genetics 2020
Quote:
... Defibrinated horse blood (TCS Bioscience) was provided using a Hemotek membrane feeding system (Hemotek Ltd) covered with Parafilm (Bemis).
-
No products found
because this supplier's products are not listed.
Michael Stitz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... The polysome profiles were generated by continuous absorbance measurement at 260 nm using a Gradient Fractionation System (Biocomp Instruments), eight fractions collected ...
-
No products found
because this supplier's products are not listed.
Gergely Tarcsay, et al.,
bioRxiv - Neuroscience 2022
Quote:
... KA was injected supra-hippocampally using a custom microinjection system (Zhu, Eom, & Hunt, 2019) (MO-10, Narishige International, USA). The glass pipette was lowered into the brain (DV ...
-
No products found
because this supplier's products are not listed.
GAC Singer, et al.,
bioRxiv - Genomics 2019
Quote:
Filtration and DNA extraction was done in PCR clean laminar flow hoods (AirClean Systems) thoroughly decontamination with ELIMINase (Decon Labs) and 70% EtOH prior to each set of three sample replicates ...
-
No products found
because this supplier's products are not listed.
Liyuan Guo, Bo Jin, Yidan Zhang, Jing Wang,
bioRxiv - Genetics 2019
Quote:
Total RNA was extracted from HEK293 cells via the RNApure Tissue/Cell Kit (Cat. No. CW0560, CoWin Bioscience, China). RT-qPCR was performed to validate the overexpression of ARSA gene by using SYBR Green Master Mix (Cat ...
-
No products found
because this supplier's products are not listed.
Eshwar R. Tammineni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Transient transfections of C2C12 myoblasts were performed upon reaching 70% confluence using the K2 Transfection System (Biontex Laboratories GmbH, Munich, Germany) as described by the manufacturer ...
-
No products found
because this supplier's products are not listed.
Jose L. Nieto-Torres, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The in vitro kinase reaction mixtures were incubated with 350 μl clarified lysate of HeLa LC3B-KO cells (8×105 cell equivalents, prepared as described above) and glutathione-sepharose beads (Bioworld) for 3 h at 4°C on a rotator ...
-
No products found
because this supplier's products are not listed.
D.M.K. Aladin, et al.,
bioRxiv - Cell Biology 2020
Quote:
... The dissociated cells were gently pipetted 5 times to dissociate cell clumps before being deposited on a PLL-PEG-coated (SuSoS), glass-bottomed dish (IWAKI) ...
-
No products found
because this supplier's products are not listed.
Yoon-Suk Kang, James E. Kirby,
bioRxiv - Microbiology 2022
Quote:
Gryphon packaging cells (Allele Biotech, San Diego, CA) were transfected with 10 μg of pRetroX-GFPopt DNA using Lipofectamine LTX (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Takefumi Kimura, et al.,
bioRxiv - Molecular Biology 2023
Quote:
LX-2 cells were obtained from Sigma-Aldrich and HepG2 cells were procured from Cellular Engineering Technologies ...
-
No products found
because this supplier's products are not listed.
Kunal Sahasrabuddhe, et al.,
bioRxiv - Neuroscience 2020
Quote:
... This is achieved by polishing the proximal end of the array to be flat and then exposing the microwires at the proximal ends 10-20 μm by ashing in oxygen plasma using an SPI Plasma Prep III system (SPI Supplies, USA).
-
No products found
because this supplier's products are not listed.
Fernando Salgado-Polo, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Cells were trypsinized into single-cell suspensions and then 8×105 cells were incubated with 5 μl of anti-GPC6 antibody LS-C36518 (LifeSpan Bioscience) and in 4 μl of APC anti-HA antibody (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Tomotaka Okamura, et al.,
bioRxiv - Immunology 2020
Quote:
... Spot-forming cells (SFCs) were counted using the KS ELISPOT compact system (Zeiss) after a 15-min reaction with an activator mix (U-Cytech, Utrecht, Netherlands). An SFC was defined as a large black spot with a fuzzy border.
-
No products found
because this supplier's products are not listed.
Danira Toral-Rios, et al.,
bioRxiv - Neuroscience 2023
Quote:
... microglia were treated either with endotoxin-free pre-formed fibrils of human recombinant tau-441 (2N4R) P301S mutant protein (0.5μm) expressed with a Baculovirus/sf9 system (StressMarq Biosciences, Cat. No. SPR-471) or PBS in serum-free media ...
-
No products found
because this supplier's products are not listed.
Wei Wei, et al.,
bioRxiv - Biochemistry 2024
Quote:
... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150; Adiponectin: Crystal Chem, 80569 ...
-
No products found
because this supplier's products are not listed.
Shumeng Jiang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... passed through a 250 µm metal cell strainer (MIDSCI) using 1x HBSS solution (Gibco 24020-117) ...
-
No products found
because this supplier's products are not listed.
Katherine L. Leiby, et al.,
bioRxiv - Bioengineering 2022
Quote:
Primary rat lung microvascular endothelial cells (RLMVEC, VEC Technologies) were cultured on fibronectin (1 μg/cm2 ...
-
No products found
because this supplier's products are not listed.
Álvaro Inglés-Prieto, et al.,
bioRxiv - Neuroscience 2020
Quote:
... cells were treated with recombinant human GDNF (Shenandoah Biotechnology) and human GFRα1 (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Alexandra-Madelaine Tichy, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... cells were incubated with 10 μM PTX (List Labs) overnight (o/n ...
-
No products found
because this supplier's products are not listed.
Takao Fujisawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
... the cells were mounted with Fluoromount (Diagnostic BioSystems, K024) for confocal microscopy ...
-
No products found
because this supplier's products are not listed.
J.I. Hoare, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... All cell lines underwent 16 locus STR verification (DNA Diagnostics Centre ...
-
No products found
because this supplier's products are not listed.
Nana Naetar, et al.,
bioRxiv - Cell Biology 2020
Quote:
... cells were stained with an anti-mEos2 antibody (Badrilla, Leeds, UK) following the standard IF protocol.
-
No products found
because this supplier's products are not listed.
Noah R. Johnson, et al.,
bioRxiv - Neuroscience 2021
Quote:
... TgF344 rat cells were labeled with chicken anti-tau (PhosphoSolutions, 1:1,000), rabbit anti-Aβ (OC ...
-
No products found
because this supplier's products are not listed.
Biao Huang, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 20,000 cells per well were seeded in iMatrix-511 (Nacalai USA, # 892021) coated Seahorse miniplates 18-20 hours before the assay ...
-
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Sean P Harrison, et al.,
bioRxiv - Cell Biology 2020
Quote:
... depending on the cell line and 3 or 4 μM CHIR99021 (BOC Sciences). Optimal conditions need to be established for each line based on our previously established protocol 4 ...
-
No products found
because this supplier's products are not listed.
Rhalena A. Thomas, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Cells were incubated with primary antibodies: MAP2 (1:1000, EnCor Biotech CPCA-MAP2); Nestin (1:500 ...
-
No products found
because this supplier's products are not listed.
Jason A Iskarpatyoti, et al.,
bioRxiv - Cell Biology 2021
Quote:
... supernatants were collected and cell pellet was lysed in 0.1% Triton-100X (Spectrum Chemical) in Tyrode’s buffer ...
-
No products found
because this supplier's products are not listed.
Benjamin Ng, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... the cell culture supernatant was concentrated using a Polyethylene glycol concentrating solution (90626, Chondrex) and collagen content was quantified using a Sirius red collagen detection kit (9062 ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... Cell supernatants were screened by ELISA using recombinant YFV E protein (Meridian Life Sciences). Wells with positive reactivity were fused to a human-mouse myeloma cell line (HMMA 2.5 ...
-
No products found
because this supplier's products are not listed.
Anupama Shirali, et al.,
bioRxiv - Immunology 2023
Quote:
... IgG control for the cell-penetration assay was obtained from Leinco technologies (I-118).
-
No products found
because this supplier's products are not listed.
Shiyu Liang, et al.,
bioRxiv - Immunology 2023
Quote:
... The cells were stained with an anti-EROS FITC-conjugated antibody (CSB-PA859832LC01HU; CUSABIO Technology ...
-
No products found
because this supplier's products are not listed.
Md Imtiaz Khalil, Arrigo De Benedetti,
bioRxiv - Cancer Biology 2022
Quote:
LNCaP and PC3 cells were treated with 1-5 μM of GLPG 0259 (Medkoo Biosciences, Inc. ...
-
No products found
because this supplier's products are not listed.
Mitsuhiro Abe, et al.,
bioRxiv - Cell Biology 2024
Quote:
... the cells were incubated with 10 nM HaloTag HMSiR Ligand (#A201-01, GORYO Chemical, Inc.) in DMEM supplemented with 0.2% BSA for 16 h ...
-
No products found
because this supplier's products are not listed.
Elisa Jentho, et al.,
bioRxiv - Immunology 2019
Quote:
Stock solution of Heme (Sigma-Aldrich for cell culture experiments and Frontier Scientific for animal experiments) and Protoporphyrin 9 (PPIX ...
-
No products found
because this supplier's products are not listed.
Aditi Madan, et al.,
bioRxiv - Physiology 2023
Quote:
... cells were incubated in Schneider’s Insect Medium without Amino Acids (United States Biological, Cat# S0100-01.10) for the indicated amount of time ...
-
No products found
because this supplier's products are not listed.
Lijun Cong, et al.,
bioRxiv - Microbiology 2021
Quote:
... prior to cell supernatants being collected for quantification of virus production by HIV-1 p24 ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
Qiyu Chen, et al.,
bioRxiv - Developmental Biology 2022
Quote:
Neurohypophyseal cells were enriched by intraperitoneally injecting 5µl of 4.6mM β-Ala-Lys-Nε-AMCA (Biotrend #BP0352) into 37-day-old juvenile ...
-
No products found
because this supplier's products are not listed.
Jason Small, Alison Weiss,
bioRxiv - Microbiology 2021
Quote:
... Cells were washed with PBST and placed in primary antibody (Rabbit Anti-E. coli, cat. 1001, ViroStat, Rabbit Anti-Intimin ...
-
No products found
because this supplier's products are not listed.
Fernando R. Balestra, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Ultra-thin (50 nm thick) serial sections were cut through the entire cell with a diamond knife (Diatome) and ultramicrotome (Leica Microsystems ...