-
No products found
because this supplier's products are not listed.
Andrew B Janowski, et al.,
bioRxiv - Microbiology 2022
Quote:
... the virus RNA was isolated from the virus stocks using Direct-zol RNA MicroPrep (Zymo research) and used for RT-PCR and Sanger sequencing of the entire virus genome.
-
No products found
because this supplier's products are not listed.
Andrew Sawyer, et al.,
bioRxiv - Immunology 2022
Quote:
... Antigen retrieval was performed by boiling samples to 100°C for 15 minutes in pH 9 Antigen retrieval buffer (Akoya). Tissues were incubated for 45 minutes at room temperature with primary antibodies specific for CD20 (L26 ...
-
No products found
because this supplier's products are not listed.
Kim S. Friedmann, et al.,
bioRxiv - Immunology 2020
Quote:
... Afterwards antigen specific CD8+ T cells were isolated using IFN-γ secretion assay (Miltenyi Biotec). Single cells were seeded into individual wells (1 cell/200 μl in each well ...
-
No products found
because this supplier's products are not listed.
Jong Hyuk Kim, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Cell line authentication for canine cells were conducted by IDEXX BioAnalytics (Columbia ...
-
No products found
because this supplier's products are not listed.
Timothy Morris, et al.,
bioRxiv - Cell Biology 2021
Quote:
ShRNA for canine synaptopodin cloned into puromycin-selectable pRS mammalian expression vector (Origene) has previously been described (Kannan and Tang ...
-
No products found
because this supplier's products are not listed.
Alyssa N Coyne, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Antigen retrieval was performed using Tissue-Tek antigen retrieval solution (IHC World) for 1 hour in a steamer ...
-
No products found
because this supplier's products are not listed.
Manutsanun Inthawong, et al.,
bioRxiv - Immunology 2022
Quote:
... A T cell antigen control pool (CEF, Mabtech AB, 3616-1) at a final concentration of 1 μg/ml was used as a source of control antigens ...
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No products found
Adam K. Wade-Vallance, et al.,
bioRxiv - Immunology 2022
Quote:
... Biosearch Technologies]) or control antigen (ovalbumin; OVA [Worthington Biochemicals]) in 20µl PBS by subcutaneous injection ...
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No products found
because this supplier's products are not listed.
Jianlong Zhai, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the antigen-antibody complexes were detected using Odyssey XF (LI-COR, USA). The signal intensities of target bands were analyzed using ImageJ software (National Institutes of Health ...
-
No products found
because this supplier's products are not listed.
Carlos A. Rodriguez, et al.,
bioRxiv - Microbiology 2023
Quote:
... Anti-canine IgG conjugated with phycoerythrin (Rockland, Inc., Limerick, PA) was used as a secondary antibody at a working dilution of 1:100 ...
-
No products found
because this supplier's products are not listed.
Andrea R. Shiakolas, et al.,
bioRxiv - Immunology 2021
Quote:
... AviTagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
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No products found
Yang Li, et al.,
bioRxiv - Immunology 2021
Quote:
PAOC cells were immortalized with Lenti-hTERT virus (ABM; cat# G200) following manufacturer’s instructions and clonal sorting/expansion ...
-
No products found
because this supplier's products are not listed.
Paul Castillo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Synthetic mRNA encoding for full-length model antigen (i.e., CleanCap GFP, catalog L-7601 and CleanCap ovalbumin, OVA, catalog L-7610) were purchased from TriLink Biotechnologies (San Diego ...
-
No products found
because this supplier's products are not listed.
Zeqing Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Antigen retrieval was performed with Antigen Retrieval Solution (Solarbio, C1035) before the conventional immunostaining procedure.
-
No products found
because this supplier's products are not listed.
Yanzhao Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The p24 antigen levels in viral supernatants were measured by an HIV-1 p24 antigen capture ELISA (XpressBio). Transfection efficiencies were normalized to the luciferase activity ...
-
No products found
because this supplier's products are not listed.
Jesse M. Hall, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antigen specific antibody titers to PT (List Biological Laboratories #180) were measured by coating ELISA plates with 50 μl of antigen per well ...
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No products found
because this supplier's products are not listed.
Nuno Apóstolo, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1:50 donkey anti-mouse IgG antigen-binding fragments (Jackson ImmunoResearch). Sections were then washed in PBS-0.5%Triton X-100 at RT and incubated at 4°C overnight with primary antibodies in PBS containing 5% NHS ...
-
No products found
because this supplier's products are not listed.
Joshua J. Sims, et al.,
bioRxiv - Genetics 2021
Quote:
... We measured reporter virus transduction activity on a luminometer (BioTek) using the Renilla Glo Kit (Promega ...
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No products found
because this supplier's products are not listed.
Marije Risselada, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... and three had canine plasma with dipotassium EDTA (K2-EDTA) (IGCNPLAK2E500ml Canine Plasma lot 41273, Innovative research, Novi, MI). A 10 multi-position hotplate with magnetic stir bars was used (RT 10 ...
-
No products found
because this supplier's products are not listed.
Tomaz Martini, et al.,
bioRxiv - Neuroscience 2020
Quote:
... antigen retrieval and antibody staining (anti-PER2, Alpha Diagnostic, cat ...
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No products found
because this supplier's products are not listed.
Qixiang He, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The virus titers were measured by flow cytometry assay (Expression Systems).
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No products found
because this supplier's products are not listed.
Md. Alamgir Hossain, et al.,
bioRxiv - Immunology 2022
Quote:
... incubated with each antigen at certain concentration in PBS/2% FCS and transfer to a 37°C thermomixer (Eppendorf). Cells were collected after each period of incubation and fixed in IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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No products found
because this supplier's products are not listed.
BI Reinfeld, et al.,
bioRxiv - Cancer Biology 2020
Quote:
PyMT GEMM mice were bred by crossing male transgenic mice expressing the polyoma virus middle T antigen (PyMT) oncoprotein under the MMTV-LTR (Jackson Laboratory 022974) with wildtype females on a similar B6/FVB mixed background ...
-
No products found
because this supplier's products are not listed.
Julianne Meisner, et al.,
bioRxiv - Microbiology 2022
Quote:
... 3) anti dog IgG-HRP as linker (Southern BioTech goat anti-canine IgG) and 4 ...
-
No products found
because this supplier's products are not listed.
Jian Hang Lam, et al.,
bioRxiv - Immunology 2022
Quote:
... with Trans IT-Virus GEN (Mirus). In brief ...
-
No products found
because this supplier's products are not listed.
Caroline E. Dewar, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The antibody was affinity purified against the antigen by Eurogentec, and used at a 1:50 dilution ...
-
No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Antigen for immunization was prepared by mixing NP-OVA (Biosearch Technologies), 10% KAL(SO4)2 dissolved in PBS at a ratio of 1:1 ...
-
No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Gururaj Shivange, et al.,
bioRxiv - Biochemistry 2021
Quote:
... For binding analysis biotinylated antigen (1 μg/mL) were immobilized on streptavidin (SA) biosensors (Pall) for 300 sec to ensure saturation ...
-
No products found
because this supplier's products are not listed.
Tamami Suzuki, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Canine aortic endothelial cells (CnAOEC: #Cn304-05, Cell Applications, CA, USA), seven canine HSA cell lines (JuA1 ...
-
No products found
because this supplier's products are not listed.
Teresa R. Wagner, et al.,
bioRxiv - Immunology 2023
Quote:
... purified antigen (Acrobiosystems) was reacted with Sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Federico Giannitti, et al.,
bioRxiv - Pathology 2022
Quote:
... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
-
No products found
because this supplier's products are not listed.
Yuan Zhang, Zhigang Liu, Bin Zhang,
bioRxiv - Cell Biology 2022
Quote:
... FVIII antigen was quantified by ELISA using the VisuLize FVIII antigen kit from Affinity Biologicals (Ancaster, ON, Canada). FV antigen was quantified by ELISA using matched-pair antibody set for human FV antigen from Affinity Biologicals ...
-
No products found
because this supplier's products are not listed.
Qiulong Yan, et al.,
bioRxiv - Microbiology 2020
Quote:
The DNA and RNA of virus were extracted by using TIANamp Virus DNA / RNA Kit (TIANGEN) according to the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIV antigen GP120 was purchased from Immune Technology Corporation (IT-001-022p) ...
-
No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
-
No products found
because this supplier's products are not listed.
Katrin Manske, et al.,
bioRxiv - Bioengineering 2023
Quote:
20,000 Human Embryonic Kidney cells (HEK293-CD19+) expressing the CD19 antigen were seeded on a 96-well E-plate (ACEA Biosciences) over night ...
-
No products found
because this supplier's products are not listed.
Wei Deng, et al.,
bioRxiv - Microbiology 2020
Quote:
... an antigen retrieval kit (AR0022, Boster) was applied to the sections at 37°C for 1 min ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yitzhak Reizel, et al.,
bioRxiv - Genetics 2020
Quote:
... and loaded into a 2100 Antigen Retriever (Proteogenix). After reaching the target temperature ...
-
No products found
because this supplier's products are not listed.
Pengxiang Chang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in HBS-EP buffer (TEKnova) containing 10 μM oseltamivir carboxylate (Roche ...
-
No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
-
No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
-
No products found
because this supplier's products are not listed.
Deepanwita Pal, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Antigen retrieval using citrate buffer for the ColI staining was performed using the PELCO Biowave microwave (Ted Pella, Inc). A monoclonal antibody for My32 (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
-
No products found
because this supplier's products are not listed.
Jakob Kreye, et al.,
bioRxiv - Immunology 2020
Quote:
... The presence of immobilized antigens was confirmed by incubation with HRP-conjugated anti-rabbit IgG (Dianova). Assays for concentration-dependent RBD binding (Fig ...
-
No products found
because this supplier's products are not listed.
William B. Klimstra, et al.,
bioRxiv - Microbiology 2020
Quote:
... 2 ml/well of virus growth medium containing 0.1% (w/v) immunodiffusion agarose (MP Biomedicals) was added and incubation was continued for 96 hours ...
-
No products found
because this supplier's products are not listed.
Kate A Parham, et al.,
bioRxiv - Immunology 2022
Quote:
Multivalent MOG-bead antigens were generated using non-functionalized 1 μm diameter silica beads (Bangs Laboratories, Inc. (SS04000) using the manufacturer’s protocol ...