-
No products found
because this supplier's products are not listed.
Xiaoyu Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... or anti- CTLA-4-APC (Mouse IgG2a, Biolegend) at 37°C for 1hour ...
-
No products found
because this supplier's products are not listed.
Anaïs Cardon, et al.,
bioRxiv - Immunology 2024
Quote:
... and 200µg of anti-mouse CTLA-4 antibody (9H10 clone, BioXCell), using purified Rat IgG2a,κ isotype control antibody (RTK2758 clone ...
-
No products found
because this supplier's products are not listed.
Alan Kennedy, et al.,
bioRxiv - Immunology 2022
Quote:
... CHO cells expressing CTLA-4 WT or CTLA-4 Del36 were surface CTLA-4 stained on ice with anti-CTLA4-PE (BD Biosciences, clone BNI3) for 60 min ...
-
No products found
because this supplier's products are not listed.
Mengdi Guo, et al.,
bioRxiv - Cancer Biology 2023
Quote:
sgRNA sequences against mouse CTLA-4 - TrueGuide™ Synthetic sgRNA (Thermo Fisher Scientific) CRISPR58571_SGM
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with recombinant human CTLA-4-Fc (7268-CT, R&D Systems, Minneapolis, MN, USA) at 1 μg/mL overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
David Knorr, et al.,
bioRxiv - Immunology 2023
Quote:
... human CTLA-4 (Sino Biological, 11159-HNAH), and human FcyRs (human FCGR2A #10374-H08H ...
-
No products found
because this supplier's products are not listed.
Wei Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... bound ACE2-mouse Fc was detected by HRP-conjugated anti mouse IgG (Fc specific) (Sigma-Aldrich). Another way to do hACE2 competition ELISA is to incubate the fixed concentration of biotinylated hACE2-Fc with gradient concentrations of antibody ...
-
No products found
because this supplier's products are not listed.
Peng Wu, Ningning Yang, Mingguo Xu, Chuangfu Chen,
bioRxiv - Cell Biology 2021
Quote:
... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
-
No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Shouyan Deng, et al.,
bioRxiv - Immunology 2022
Quote:
... CTLA-4 (CT4-H5255; Acrobiosystems), CD28 (CD8-H525a ...
-
No products found
because this supplier's products are not listed.
Annette B. Vogel, et al.,
bioRxiv - Immunology 2020
Quote:
... Anti-mouse-Fc-antibody (Jackson ImmunoResearch) was diluted in 10 mM sodium acetate buffer pH 5 (30 µg/mL ...
-
No products found
because this supplier's products are not listed.
Panayiotis Anastasiou, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or 10 mg/kg anti-CTLA-4 (clone 9D9, cat. mctla4-mab10, InvivoGen). Antibodies were administered twice weekly for a maximum of four weeks.
-
No products found
because this supplier's products are not listed.
Susanta Chatterjee, et al.,
bioRxiv - Molecular Biology 2021
Quote:
TET-ON stable HEK293 cells with the Tetracycline-inducible expression constructs were grown in DMEM supplemented with 10% TET-approved FCS (Clontech) and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA) ...
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
CTLA-4 Blockade Bioassay was purchased from Promega (JA3001; Madison, WI, USA) and performed according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Sangsin Lee, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and mouse IgG1 Fc (Addgene #28216) and IgG2a Fc (Addgene #114492) ...
-
No products found
because this supplier's products are not listed.
Anunay J. Pulukuri, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653) in the dark at room temperature for 1 h ...
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
-
No products found
because this supplier's products are not listed.
Yuhao Shi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... pre-treatment and post-treatment/acquired resistant biopsies were obtained from patients receiving various immune checkpoint inhibitor treatments (PD-L1, PD-1, CTLA-4 targeted therapies) for RNA-seq analysis (Illumina HiSeq2500) from formalin fix paraffin embedded samples ...
-
No products found
because this supplier's products are not listed.
Giulia Ferrari, et al.,
bioRxiv - Cell Biology 2021
Quote:
... FACS data analysis was done using FCS Express 4 (De Novo Software). A similar procedure was followed for FACS cell purification ...
-
No products found
because this supplier's products are not listed.
Shreya Ahuja, Iulia M. Lazar,
bioRxiv - Immunology 2021
Quote:
... TGFβ1 (HEK293 derived) and TGFβ2 (HEK293 derived) were acquired from Peprotech (Rocky Hill, NJ). Primary antibody [mouse monoclonal IgG anti-Vimentin (V9)] ...
-
No products found
because this supplier's products are not listed.
Sophie D. Adams, et al.,
bioRxiv - Cell Biology 2020
Quote:
... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Sonal Jaiswal, Srishti Sanghi, Priyanka Singh,
bioRxiv - Cell Biology 2023
Quote:
... and anti-mouse IgG (Fc) TRITC (Merck, #SAB3701020, 1:500).
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No products found
because this supplier's products are not listed.
Christian L. Egly, et al.,
bioRxiv - Biochemistry 2024
Quote:
... All HEK293 cells were cultured in HEK293 media consisting of Minimum Essential Media (MEM, Corning) containing 10% (v/v ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
P.K Smitha, et al.,
bioRxiv - Molecular Biology 2022
Quote:
IgGFc: Human IgG Fc Sequence (Supplementary Material Figure S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
-
No products found
because this supplier's products are not listed.
Shingo Hanaoka, Shinji Saijou, Yasuhiro Matsumura,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... reacted with HRP-conjugated anti-human IgG-Fc antibody or anti-mouse IgG-Fc antibody (Bethyl) for 1 hour ...
-
No products found
because this supplier's products are not listed.
Laurent Gros, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
-
No products found
because this supplier's products are not listed.
Anna L Koessinger, et al.,
bioRxiv - Cancer Biology 2021
Quote:
For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
-
No products found
because this supplier's products are not listed.
Eileen Shiuan, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... samples were blocked in αCD16/32 mouse Fc block (Tonbo Biosciences #70-0161) and stained for extracellular proteins using an antibody master mix made in buffer ...
-
No products found
because this supplier's products are not listed.
Ryan E. Yoast, et al.,
bioRxiv - Cell Biology 2021
Quote:
Total mRNA was isolated from HEK293 (2×106) and Jurkat cells (4×106) parental and MCU-KO cell lines using the RNeasy Mini Kit (Qiagen). Isolated RNA was then analyzed using a NanoDrop spectrophotometer (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Tanne van der Wal, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Membranes were stored at 4°C in TBS and imaged on an Odyssey Fc (LI-COR) for two minutes at 680 nm and 800 nm ...
-
No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Biochemistry 2023
Quote:
HEK293 cells were cultured in DMEM (PAN Biotech). All media were supplemented in with 10% FBS (PAN Biotech ...
-
No products found
because this supplier's products are not listed.
Andrea J. Pruijssers, et al.,
bioRxiv - Microbiology 2020
Quote:
... or 10% FCS fetal clonal serum (FCS)(HyClone, GE Life Sciences), 100 U/ml penicillin and streptomycin (Gibco ...
-
No products found
because this supplier's products are not listed.
Xiyuan Bai, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-CTLA-4 neutralizing antibody and non-immune human IgG antibody were purchased from BPS Bioscience Inc (San Diego ...
-
No products found
because this supplier's products are not listed.
Mathieu C. Husser, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Tagged HEK293 cells were seeded directly onto 4-well μ-slides (Ibidi) for imaging ...
-
No products found
because this supplier's products are not listed.
Xiao Ma, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... HEK293/GC-A+ and HEK293/GC-B+ cell lines were generated from HEK293 parental cell transfected with plasmids (OriGene, Rockville, MD) containing either human GC-A or GC-B cDNA sequences ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Jaehyeon Jeong, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... HEK293 lysates were collected using RIPA buffer (Cell Signaling Technology), and the protein concentrations of the lysates were quantified through Bradford assays (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Zane G. Moreland, et al.,
bioRxiv - Cell Biology 2021
Quote:
Mouse inner ears were fixed in 4% paraformaldehyde (EMS Diasum) in PBS for 2 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Kuan-Ting Huang, et al.,
bioRxiv - Biochemistry 2022
Quote:
Fluorescence stereomicroscope (e.g., Leica M165 FC)
-
No products found
because this supplier's products are not listed.
Justin B. Hackett, et al.,
bioRxiv - Immunology 2022
Quote:
... and 100 μg anti CTLA-4 (clone 9D9, Leinco Technologies) in volume of 200 μg 1X PBS.
-
No products found
because this supplier's products are not listed.
Nicolò Maganzini, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Mouse Fc-capture biosensor tips were purchased from Sartorius. Chicken plasma (cat # ...
-
No products found
because this supplier's products are not listed.
Jordana Griffiths, et al.,
bioRxiv - Immunology 2020
Quote:
... For domain binding determination PE-labelled secondary anti-mouse Fc antibody (Jackson Laboratories) was added ...
-
No products found
because this supplier's products are not listed.
Qin Huang, et al.,
bioRxiv - Neuroscience 2019
Quote:
... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
-
No products found
because this supplier's products are not listed.
Xinyuan Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Izumi Sasaki, et al.,
bioRxiv - Immunology 2023
Quote:
... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Saskia D. van Asten, et al.,
bioRxiv - Immunology 2020
Quote:
... 1% FCS and 12.5µg/ml DNAse (Roche) in IMDM (Gibco) ...