-
No products found
because this supplier's products are not listed.
Iosifina P. Foskolou, et al.,
bioRxiv - Immunology 2022
Quote:
... CTLA-4 (UC10-4B9, Biolegend); FcR Block (120-000-826 ...
-
No products found
because this supplier's products are not listed.
Amy J. Wisdom, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and anti-CTLA-4 (BioXCell, BE0164) or isotype control (MSD IgG1 control for muDX400 ...
-
No products found
because this supplier's products are not listed.
Musleh M. Muthana, et al.,
bioRxiv - Immunology 2023
Quote:
... Polyhistidine-tagged human CTLA-4 (HIS-hCTLA-4) (Sino Biological Inc, 11159-H08H). Mouse TNF-alpha (Sino Biological Inc ...
-
No products found
because this supplier's products are not listed.
Shouyan Deng, et al.,
bioRxiv - Immunology 2022
Quote:
... CTLA-4-PE (12-1522-82, Invitrogen), CD39-PE (12-0391-82 ...
-
No products found
because this supplier's products are not listed.
Emily F. Davis-Marcisak, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Flow antibodies included anti-human CD152 (CTLA-4) (BD Bioscience 555853), CD28 (Biolegend 302907) ...
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with recombinant human CTLA-4-Fc (7268-CT, R&D Systems, Minneapolis, MN, USA) at 1 μg/mL overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Filip Mihalic, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
-
No products found
because this supplier's products are not listed.
Shouyan Deng, et al.,
bioRxiv - Immunology 2022
Quote:
... CTLA-4 (CT4-H5255; Acrobiosystems), CD28 (CD8-H525a ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
-
No products found
because this supplier's products are not listed.
Alexander Kuzin, et al.,
bioRxiv - Genomics 2021
Quote:
... human Hek293 DNA was purchased from Genscript. S ...
-
No products found
because this supplier's products are not listed.
Panayiotis Anastasiou, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or 10 mg/kg anti-CTLA-4 (clone 9D9, cat. mctla4-mab10, InvivoGen). Antibodies were administered twice weekly for a maximum of four weeks.
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Mélanie Gasser, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
-
No products found
because this supplier's products are not listed.
Rosemary A. Bamford, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 1x penicillin/streptomycin (pen/strep; PAA) and distributed in 6-well plates with HEK293 cells transfected with Neo1.a-AP-His (Addgene #71963) or pcDNA3.1-CNTN4-FLAG ...
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
-
No products found
because this supplier's products are not listed.
Erica L. Stone, et al.,
bioRxiv - Immunology 2021
Quote:
CTLA-4 Blockade Bioassay was purchased from Promega (JA3001; Madison, WI, USA) and performed according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Weihua Qin, et al.,
bioRxiv - Cell Biology 2022
Quote:
... His- tagged human UHRF1 was purified using Ni-NTA sepharose resin (Qiagen). Recombinant E1 (His-UBE1) ...
-
No products found
because this supplier's products are not listed.
Xiyuan Bai, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-CTLA-4 neutralizing antibody and non-immune human IgG antibody were purchased from BPS Bioscience Inc (San Diego ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Kazuhiro Yamamoto, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human HMGB2 (C-terminal His tag, TP720732) from ORIGENE (Rockville, MD); anti-rabbit alkaline phosphatase-linked antibody ...
-
No products found
because this supplier's products are not listed.
Xinyuan Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
-
No products found
because this supplier's products are not listed.
Chloe G Myers, et al.,
bioRxiv - Cell Biology 2024
Quote:
Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Yuhao Shi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... pre-treatment and post-treatment/acquired resistant biopsies were obtained from patients receiving various immune checkpoint inhibitor treatments (PD-L1, PD-1, CTLA-4 targeted therapies) for RNA-seq analysis (Illumina HiSeq2500) from formalin fix paraffin embedded samples ...
-
No products found
because this supplier's products are not listed.
Isadora A. Oliveira, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4 °C with anti-His (Santa Cruz Biotechnologies, USA). The membrane was then washed ...
-
No products found
because this supplier's products are not listed.
Bashir A. Ali, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or anti-His (Cell Signaling #2366 His-Tag 27E8) primary antibodies ...
-
No products found
because this supplier's products are not listed.
Yaobin Liu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... His (Roche), GRASP55 (Proteintech) ...
-
No products found
because this supplier's products are not listed.
Anna L Koessinger, et al.,
bioRxiv - Cancer Biology 2021
Quote:
For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
-
No products found
because this supplier's products are not listed.
Andrew J. Hayes, et al.,
bioRxiv - Microbiology 2021
Quote:
... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Rita Turpin, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and 100ng/mL Human IL-4 (Miltenyi Biotec) for 5 days only adding media once in between ...
-
No products found
because this supplier's products are not listed.
Neil D. Butler, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech. Oligonucleotides were purchases from IDT ...
-
No products found
because this supplier's products are not listed.
Jack Llewellyn, et al.,
bioRxiv - Cell Biology 2021
Quote:
Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Stefania Capone, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Mathieu C. Husser, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Tagged HEK293 cells were seeded directly onto 4-well μ-slides (Ibidi) for imaging ...
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
Qin Huang, et al.,
bioRxiv - Neuroscience 2019
Quote:
... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Vinita Bharat, et al.,
bioRxiv - Cell Biology 2022
Quote:
Human neurons were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 15 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Michelle E. Gray, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
-
No products found
because this supplier's products are not listed.
Isabel Koh, Masaya Hagiwara,
bioRxiv - Bioengineering 2022
Quote:
Human iPSCs (IMR90-4; WiCell Research Institute) were maintained with mTeSR Plus (STEMCELL Technologies, 100-0276) on dishes coated with 9-10 μg/cm2 Growth Factor Reduced Matrigel (Corning ...
-
No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Dilutions of human serum (Human Serum age 4-6, Innovative Research) were performed in TBSM ...
-
No products found
because this supplier's products are not listed.
Astrid Hendriks, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4°C) and incubated with goat anti-human IgG-Alexa Fluor 647 and goat anti-human IgM-PE (both Southern Biotech) for 30 minutes at 4°C at 600 rpm ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...