-
No products found
because this supplier's products are not listed.
Darwin W. Kwok, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and anti-CD137 antibody (4-1BB; Biolegend Cat #309804) to assess CD8+ T-cell degranulation and TCR activation ...
-
No products found
because this supplier's products are not listed.
Devin Dersh, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and 4-1BB/CD137 to monitor T-cell activation (clone 4B4-1, APC, BD).
-
No products found
because this supplier's products are not listed.
Luiz Henrique Geraldo, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and anti-4-1BB (clone LOB12.3, BioXCell) treatment ...
-
No products found
because this supplier's products are not listed.
Suzanne M. Castenmiller, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and then stained with anti-CD137 Alexa647 (Invitrogen), anti-CD154 BV510 ...
-
No products found
because this supplier's products are not listed.
Elisa Claeys, et al.,
bioRxiv - Immunology 2021
Quote:
... OX40 and 4-1BB reporter constructs were cloned from vectors purchased from Sino Biological. Sequences were confirmed by automated capillary Sanger sequencing (Macrogen Europe).
-
No products found
because this supplier's products are not listed.
Caitlin M. MacCarthy, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... cynomolgus and porcine fibroblasts were cultured in high-glucose DMEM (Sigma) supplemented with 15% FBS ...
-
No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and cynomolgus IL11 were measured on Biacore T200 (GE Healthcare). VVB011 was immobilised on an anti-mouse capture chips and a concentration range of each of the analytes (hIL11 ...
-
No products found
because this supplier's products are not listed.
Joseph R. Palmeri, et al.,
bioRxiv - Immunology 2023
Quote:
... α4-1BB or α4-1BB-LAIR was administered intratumorally (i.t.) in 20 μL of sterile PBS (Corning) at a dose of 30 μg or 36.1 μg (molar equivalents) ...
-
No products found
because this supplier's products are not listed.
Gwen Swinnen, et al.,
bioRxiv - Plant Biology 2021
Quote:
... whereas selective media additionally lacked His (−4) (Clontech).
-
No products found
because this supplier's products are not listed.
Yuan Wang, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 4-1BB (Acrobiosystems) or BSA (Solarbio ...
-
No products found
because this supplier's products are not listed.
Eswari Dodagatta-Marri, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and 4-1BB (MAB9371, R&D systems) 1mg/kg was injected on days 0 and 4 through an intravenous route ...
-
No products found
because this supplier's products are not listed.
Ruhang Tang, et al.,
bioRxiv - Physiology 2019
Quote:
... anti-CD137 rabbit polyclonal antibody (1:1000, Abcam, ab203391), total OXPHOS rodent WB antibodies (Abcam ...
-
No products found
because this supplier's products are not listed.
Gamze Gulden, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The lentiviral vector encoding CD19 specific CAR (CD19-CD8-4-1BB-CD3z) was designed and synthesized by GenScript. The envelope pCMV-VSV-G plasmid (from Bob Weinberg (Addgene #8454 ...
-
No products found
because this supplier's products are not listed.
Rosemary A. Bamford, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 1x penicillin/streptomycin (pen/strep; PAA) and distributed in 6-well plates with HEK293 cells transfected with Neo1.a-AP-His (Addgene #71963) or pcDNA3.1-CNTN4-FLAG ...
-
No products found
because this supplier's products are not listed.
Sharanjot Saini, et al.,
bioRxiv - Genetics 2020
Quote:
... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
-
No products found
because this supplier's products are not listed.
Ravi Jagasia, et al.,
bioRxiv - Neuroscience 2022
Quote:
NPCs derived from cynomolgus macaque (Cips_BC671A_NSC line generated at Roche) were differentiated in NPC differentiation medium (42) ...
-
No products found
because this supplier's products are not listed.
Masayoshi Kukida, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Total RNA of the renal cortex in cynomolgus monkeys was extracted using Maxwell RSC simplyRNA tissue kit (Promega, AS1340) by an automated Maxwell RSC Instrument (Promega) ...
-
No products found
because this supplier's products are not listed.
Isadora A. Oliveira, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4 °C with anti-His (Santa Cruz Biotechnologies, USA). The membrane was then washed ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Bashir A. Ali, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or anti-His (Cell Signaling #2366 His-Tag 27E8) primary antibodies ...
-
No products found
because this supplier's products are not listed.
Anna L Koessinger, et al.,
bioRxiv - Cancer Biology 2021
Quote:
For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
-
No products found
because this supplier's products are not listed.
Andrew J. Hayes, et al.,
bioRxiv - Microbiology 2021
Quote:
... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
-
No products found
because this supplier's products are not listed.
Rosario Francisco-Velilla, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... or anti-His (Merck) for His-G5845–1508) ...
-
No products found
because this supplier's products are not listed.
Amy Q. Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and male Cynomolgus monkey plasma (BioIVT) using a Pierce Rapid Equilibration Dialysis (RED ...
-
No products found
because this supplier's products are not listed.
Benjamin J. Meckiff, et al.,
bioRxiv - Immunology 2020
Quote:
... CD137-expressing CD4+ memory T cells cells were positively selected by staining with biotin-conjugated CD137 antibody (clone REA765; Miltenyi Biotec) followed by anti-biotin MicroBeads and applied to a new MS column ...
-
No products found
because this supplier's products are not listed.
Adrian Westhaus, et al.,
bioRxiv - Bioengineering 2022
Quote:
Cynomolgus and Rhesus macaque primary hepatocytes were purchased from Lonza (Cat# MKC118 and Cat# MKR103). Human hepatocytes were also purchased from Lonza (Cat# HUM181971 ...
-
No products found
because this supplier's products are not listed.
Corinna L. Kufner, et al.,
bioRxiv - Biophysics 2022
Quote:
... The sequencing (Step 4) was performed using a Hi-Seq sequencer (Illumina, US) and the data was then filtered using the framing sequence ACAC with a quality score larger then 20 and analyzed (Step 5 ...
-
No products found
because this supplier's products are not listed.
Xinyuan Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
-
No products found
because this supplier's products are not listed.
Yoshifumi Abe, et al.,
bioRxiv - Neuroscience 2023
Quote:
... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
-
No products found
because this supplier's products are not listed.
Neil D. Butler, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech. Oligonucleotides were purchases from IDT ...
-
No products found
because this supplier's products are not listed.
Mathieu C. Husser, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Tagged HEK293 cells were seeded directly onto 4-well μ-slides (Ibidi) for imaging ...
-
No products found
because this supplier's products are not listed.
Shreya Ahuja, Iulia M. Lazar,
bioRxiv - Immunology 2021
Quote:
... TGFβ1 (HEK293 derived) and TGFβ2 (HEK293 derived) were acquired from Peprotech (Rocky Hill, NJ). Primary antibody [mouse monoclonal IgG anti-Vimentin (V9)] ...
-
No products found
because this supplier's products are not listed.
Eider Valle-Encinas, et al.,
bioRxiv - Cell Biology 2020
Quote:
... subsequently diluted in water (1:4) and mixed with SensiFAST SYBR Hi-Rox (Bioline) and the appropriate primers at a final concentration of 10 μM ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Qin Huang, et al.,
bioRxiv - Neuroscience 2019
Quote:
... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
-
No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Loyda M. Morales Rodríguez, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
HEK293 cells were plated onto 25mm coverslips (Electron Microscopy Sciences, #50949050) coated with poly-D-lysine (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
William J Dower, et al.,
bioRxiv - Immunology 2023
Quote:
... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
-
No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
-
No products found
because this supplier's products are not listed.
Xiao Ma, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... HEK293/GC-A+ and HEK293/GC-B+ cell lines were generated from HEK293 parental cell transfected with plasmids (OriGene, Rockville, MD) containing either human GC-A or GC-B cDNA sequences ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Hwoi Chan Kwon, et al.,
bioRxiv - Biophysics 2020
Quote:
Transfected HEK293 cells were identified by the green fluorescence under the Leitz DM IRB inverted microscope (Leica) equipped with GFP filter (Chroma Technology ...
-
No products found
because this supplier's products are not listed.
Timothy S. Strutzenberg, et al.,
bioRxiv - Biophysics 2019
Quote:
... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...