-
No products found
because this supplier's products are not listed.
Haiyun Yue, et al.,
bioRxiv - Microbiology 2024
Quote:
... Reactive Oxygen Species Assay Kit (DCFH-DA) was obtained from Solarbio Science & Technology (Beijing ...
-
No products found
because this supplier's products are not listed.
Natalia Akulenko, et al.,
bioRxiv - Molecular Biology 2023
Quote:
About 1.5 µg of total RNA extracted from 106 cells with Extract RNA reactive (Evrogen) was subjected to the DNAse I treatment (Ambion ...
-
No products found
because this supplier's products are not listed.
Franziska Jäger, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
No products found
because this supplier's products are not listed.
Alexandra V. Vylegzhanina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and the bound p53-reactive IgG antibodies were detected with anti-human IgG-HRP (Jackson ImmunoResearch) diluted 1000-fold in Assay Buffer for 1 hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Tyler N. Starr, et al.,
bioRxiv - Microbiology 2022
Quote:
... Proteins were biotinylated overnight at 4°C using a BirA biotinprotein ligase reaction kit (Avidity) and re-purified using the same HisTrap HP affinity method described above before being flash frozen.
-
No products found
because this supplier's products are not listed.
Fernando Navarrete, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Reactive oxygen species production was monitored by luminescence over 30-40 min in a microplate reader (Synergy H1, BioTek). At least 3 N ...
-
No products found
because this supplier's products are not listed.
Vanesa Madan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... were cultured at 27°C in Insect-XPRESS protein-free insect medium with L-Glutamine (Lonza) in shaking suspension ...
-
No products found
because this supplier's products are not listed.
Chun Su, et al.,
bioRxiv - Genomics 2019
Quote:
... pGFP-C-shRNA-Lenti-B2M and pGFP-C-scrambled were purchased from Origene. The packaging vectors PmD2G and PsPAX.2 were obtained from Addgene ...
-
No products found
because this supplier's products are not listed.
Aleksander T. Szczurek, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 1.5 (MatTek, #P35G-1.5-14-C). On the day of measurement the cells were labelled using 100nM PA-JF549-Halo (kind gift of L ...
-
No products found
because this supplier's products are not listed.
Kameron Y. Sugino, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Maternal serum samples collected at 0.6 G were analyzed for C-reactive protein (CRP) using an hsCRP ELISA kit (MP Biomedicals, Solon, OH) according to the manufacturer’s protocol with 1:100 serum dilution and were analyzed for IL-6 using an old world monkey IL-6 ELISA kit (U-CyTech Biosciences ...
-
No products found
because this supplier's products are not listed.
Jenny A. Greig, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Reactive Opal dyes were purchased from Akoya Biosciences ...
-
No products found
because this supplier's products are not listed.
Piotr T. Wysocki, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or c) supplemented with recombinant human IGFBP5 protein (500 ng/ml; Peprotech, Rocky Hill, NJ, USA). Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Jiachen Huang, Darren Diaz, Jarrod J. Mousa,
bioRxiv - Immunology 2020
Quote:
... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
-
No products found
because this supplier's products are not listed.
Santra Brenna, et al.,
bioRxiv - Neuroscience 2019
Quote:
... arabinoside-C (ARA-C, Tocris) was added in a concentration of 15µM to prevent mitotic ...
-
No products found
because this supplier's products are not listed.
Yun Huang, et al.,
bioRxiv - Biophysics 2023
Quote:
... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
-
No products found
because this supplier's products are not listed.
Simeon R. Mihaylov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
-
No products found
because this supplier's products are not listed.
Josephine W. Thinwa, et al.,
bioRxiv - Microbiology 2022
Quote:
... guinea pig anti-p62/SQSTM1 (C-terminus) (Progen, GP62-C), mouse anti-SQSTM1 (Abnova ...
-
No products found
because this supplier's products are not listed.
Amine Driouchi, et al.,
bioRxiv - Biophysics 2023
Quote:
... ribonucleoprotein (RNP) complexes included sgRNAs targeting either N- or C-terminal region (Integrated DNA Technologies - IDT) and Cas9 protein (PNA bio, Cat #CP01) were transfected together with linear dsDNA donors (IDT ...
-
No products found
because this supplier's products are not listed.
Amir Mani, et al.,
bioRxiv - Microbiology 2023
Quote:
... C and D (Illumina). Amplicon concentration across samples was normalized to 200 ng/μl using the Qubit high sensitivity dsDNA assay before pooling ...
-
No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
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Peter D. Szigetvari, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The reactive protein bands were visualized using the WesternBright ECL HRP substrate (Advansta; San Jose, USA). The blots were scanned using Gel DOC XRS+ (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Alexander J. Boys, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Wafers were placed into a PlasmaPro 80 Reactive Ion Etcher (RIE) (Oxford Instrument – Abingdon, UK) to etch out the electrodes and connector array pads ...
-
No products found
because this supplier's products are not listed.
Kristina Haase, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Reactive oxygen species were detected with ROS-ID® Hypoxia/Oxidative stress detection kit (Enzo Life Sciences) according to manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Melissa J. Mann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
-
No products found
because this supplier's products are not listed.
Margrethe A. Boyd, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... Protein expression was monitored at 37°C in a SpectraMax i3x plate reader (Molecular Devices). GFP was monitored at ex ...
-
No products found
because this supplier's products are not listed.
Kim T Melville, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein fluorescence was measured at 25 °C on a CLARIOstar multimode plate reader (BMG Labtech), with excitation at 295 ± 10 nm ...
-
No products found
because this supplier's products are not listed.
Mahta Barekatain, et al.,
bioRxiv - Biophysics 2021
Quote:
... then cut according to marker protein size and incubated with antibodies against marker proteins for mitochondria (Cytochrome c Antibody; Novus Biologicals NB100-56503), ER (SEC61B Polyclonal Antibody ...
-
Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Cat# LS001643,
5x1 mg, $166.00
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Annika Müller-Eigner, et al.,
bioRxiv - Physiology 2021
Quote:
... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
-
No products found
because this supplier's products are not listed.
Rahima Ziane, Alain Camasses, Marta Radman-Livaja,
bioRxiv - Molecular Biology 2020
Quote:
... for 1h in 37°C and proteins were then digested with Proteinase K (Euromedex, final concentration 0.4 mg/ml) for 2h at 37°C and the temperature was then shifted to 65°C overnight to reverse cross-links ...
-
No products found
because this supplier's products are not listed.
Gabriele Ciceri, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Kunihiko Yamashiro, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Whole-cell recordings were made from visually identified or fluorescent protein-positive PCs using upright and fluorescence microscopes at 32°C (BX50W1, Olympus). Patch pipettes (1.5–2.5 MΩ ...
-
No products found
because this supplier's products are not listed.
Wenxiang Zhang, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
-
No products found
because this supplier's products are not listed.
C.B. Greer, et al.,
bioRxiv - Neuroscience 2020
Quote:
... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
-
No products found
because this supplier's products are not listed.
Soumen Barman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Protein expression was induced overnight at 17°C with 0.5 mM IPTG in an Innova 44 Incubator shaker (New Brunswick Scientific). Proteins were purified as follows ...
-
No products found
because this supplier's products are not listed.
Stefano Cattaneo, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and incubated with primary antibodies diluted in blocking solution (anti-SST 1:100 v/v, Millipore, and anti-red fluorescent protein 1:400 v/v, Rockland Immunochemicals, overnight at 4°C). Slices were then washed for 1h by alternating PBS and blocking solution every 15 min ...
-
No products found
because this supplier's products are not listed.
Deike J. Omnus, et al.,
bioRxiv - Microbiology 2021
Quote:
... Samples were boiled at 65 °C for 10 minutes and separated by SDS-Page (Bio-Rad 4–20 % Mini-PROTEAN® TGX Stain-Free™ protein gel), visualized by InstantBlue protein stain (Expedeon ...
-
No products found
because this supplier's products are not listed.
Stefanie Krug, et al.,
bioRxiv - Immunology 2021
Quote:
... Protein concentrations were determined by CB-X protein assay (G-Biosciences) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Stefan Kol, et al.,
bioRxiv - Bioengineering 2019
Quote:
... protein A biosensors (Pall) were equilibrated in sample diluent buffer (PBS containing 0.1% BSA and 0.02% Tween-20) ...
-
No products found
because this supplier's products are not listed.
Paul Wieringa, et al.,
bioRxiv - Bioengineering 2019
Quote:
... via amine-reactive linker molecules of NHS-peg4-Azide (Jena Bioscience) and sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (Sulfo-SMCC ...
-
No products found
because this supplier's products are not listed.
Takashi Ando, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Cells were labeled with DCFH-DA FITC antibody using the Reactive Oxygen Species (ROS) Detection Assay Kit obtained from BioVision, Inc ...
-
No products found
because this supplier's products are not listed.
Chi Wang, et al.,
bioRxiv - Biophysics 2022
Quote:
... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
-
No products found
because this supplier's products are not listed.
Zhen Cui, et al.,
bioRxiv - Biophysics 2022
Quote:
... 986 and 987 and two alanine substitutions at residues 683 and 685 and a C-terminal T4 fibritin foldon domain to facilitate the protein expression and stabilization of the trimer conformation (ACROBiosystems, Cat No. SPN-C52Hz). All the constructs described above were attached with a C-terminal six-His for protein purification ...
-
No products found
because this supplier's products are not listed.
Victoria L. Castro, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... C-terminal HCFC1 (Bethyl labs Cat # 50- 156-0286 purchased through Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Xiangyu Shen, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... × 15 cm pulled tip C-18 column (Jupiter C-18 300 Å, 5 micron, Phenomenex). This system ran in-line with a Thermo Orbitrap Velos Pro hybrid mass spectrometer equipped with a nano-electrospray source (Thermo Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.
-
No products found
because this supplier's products are not listed.
Pablo Castro-Córdova, et al.,
bioRxiv - Microbiology 2020
Quote:
... and protein concentration was quantified by BCA protein kit (RayBiotech USA). Next ...
-
No products found
because this supplier's products are not listed.
Sangwoo Kim, et al.,
bioRxiv - Biophysics 2020
Quote:
... for a dorsal view of the tailbud and imaged at 25°C using a 40x water immersion objective (LD C-Apochromat 1.1 W, Carl Zeiss) on an inverted Zeiss Laser Scanning Confocal (LSM 710 ...
-
No products found
because this supplier's products are not listed.
Rachel J. Hagey, et al.,
bioRxiv - Microbiology 2021
Quote:
... Exceptions to these protocols were as follows: (i) RNA purification after acylation was performed using RNA C&C columns (Zymo Research), rather than ethanol precipitation ...
-
No products found
because this supplier's products are not listed.
Shiyou Zhu, et al.,
bioRxiv - Microbiology 2021
Quote:
Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...