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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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No products found
because this supplier's products are not listed.
Kelsey M. Tyssowski, Jesse M. Gray,
bioRxiv - Neuroscience 2019
Quote:
... The temperature was maintained by putting the plate on a 37°C warming plate (Bel-Art). The CO2 was maintained at 5% throughout the duration of the recording using the base provided with the Axion Lumos system ...
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No products found
because this supplier's products are not listed.
Swayam Prakash Srivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Urine albumin levels were assayed using a Mouse Albumin ELISA Kit (Exocell, Philadelphia, PA).
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No products found
because this supplier's products are not listed.
Jesse Garcia Castillo, et al.,
bioRxiv - Immunology 2024
Quote:
... Quantification of IgG for samples was done by diluting serum samples and using the Mouse IgG ELISA commercial kit (Molecular Innovations). For treatment ...
-
No products found
because this supplier's products are not listed.
Isabelle Petit-Härtlein, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 0.1 µL of protein solution and 0.1 µL of reservoir were mixed to equilibrate against 45 µL reservoir solution at 20°C in 96-well CrystalDirect plates (MiTeGen) and automatically imaged in RockImager robot (Formulatrix) ...
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No products found
because this supplier's products are not listed.
Rebecca Soffe, et al.,
bioRxiv - Microbiology 2019
Quote:
... a leaf colonising bacterium was grown overnight on nutrient agar plates (13 gL-1 Lysogeny broth and 15 gL-1 bacteriological Agar, Oxoid) containing 20 mg/L of gentamycin (AG Scientific) at 30 °C.52 The bacteria was then harvested using a sterile inoculation loop and was suspended in 5 ml of sterile phosphate buffer saline (PBS pH 7.4 ...
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No products found
because this supplier's products are not listed.
Thomas T. Thomsen, et al.,
bioRxiv - Microbiology 2021
Quote:
... from which 20 μl was spotted on solid lactose agar plates (Bromthymole Blue plates, SSI Diagnostica). Bacterial counts were recorded after overnight incubation at 37°C in ambient air ...
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No products found
because this supplier's products are not listed.
Ainelen Piazza, et al.,
bioRxiv - Microbiology 2022
Quote:
The quantification of c-di-GMP levels was performed using the Cyclic-di-GMP Assay Kit from Lucerna (Catalog Number: 200-100). Cultures of the strains were adjusted to OD600 = 0.2 and set up for the assay in 30-µl aliquots along with assay reagents and serially diluted c-di-GMP standards ...
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No products found
because this supplier's products are not listed.
Thanh Hoang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Platinum plate electrode tweezers (Protech International Inc.) were used to deliver two 50 ms pulses (75 V with a 1 s pause between pulses ...
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No products found
because this supplier's products are not listed.
Julia L. Daiß, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Top2a was obtained from Inspiralis (c/n HT210). Proteins were incubated together in pulldown buffer (25 mM TrisHCl pH7.9 ...
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No products found
because this supplier's products are not listed.
Yuichiro Honda, et al.,
bioRxiv - Pathology 2020
Quote:
... The sections were blocked with 5% bovine serum albumin in PBS for 60 min and incubated overnight at 4°C with a mouse anti-CD-11b primary antibody (1:2000; BMA Biomedicals, Augst, Switzerland) or a rabbit polyclonal anti-dystrophin primary antibody (1:1000 ...
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No products found
because this supplier's products are not listed.
Valentin Mitterer, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Growth phenotypes of the mutant alleles were analysed on plates containing 1 g/l 5-fluoroorotic acid (5-FOA) (Apollo Scientific, Cat# PC4054) to select for cells that have lost the wild-type SPB4-containing URA3 plasmid ...
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No products found
because this supplier's products are not listed.
Mohamad Ibrahim Cheikh, et al.,
bioRxiv - Biophysics 2022
Quote:
... plates were covered in light halocarbon oil (Halocarbon Oil 27, Sigma). Embryos with a distinctive faint halo in the periphery ...
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No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
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No products found
because this supplier's products are not listed.
Stephanie N. Langel, et al.,
bioRxiv - Immunology 2021
Quote:
... the plate was washed and SULFO-TAG (MSD) anti-hamster IgA (Brookwood Biomedical) detection antibody was added ...
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No products found
because this supplier's products are not listed.
Chang-Yu Chen, et al.,
bioRxiv - Immunology 2021
Quote:
... The endotoxin level of HMGN1 (< 1 endotoxin unit per mg) was assessed by Endospecy ES-50M Kit (Seikagaku Corporation, Japan). The protein sequence of HMGN1 was identified by Applied Biosystems Procise 492 HT (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Sunada Khadka, et al.,
bioRxiv - Biochemistry 2022
Quote:
... hygromycin resistance marker and red fluorescent protein (RFP) were purchased from Cellecta. For transfection ...
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No products found
because this supplier's products are not listed.
Y Audrey Hay,
bioRxiv - Neuroscience 2021
Quote:
... France) mice temperature was monitored and maintained at 34-36 °C using a heating blanket (Basi). A small burr hole was drilled at LDTg coordinates AP = -4.92 mm and ML = 1 mm and an optical fiber (300 μm ...
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No products found
because this supplier's products are not listed.
Keito Okazaki, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... A Histofine Kit (Nichirei Biosciences), which employs the streptavidin-biotin amplification method ...
-
No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... plastic culture plates were coated with 20% Matrigel Matrix (CB40230C) and cells were plated in plating medium (BioIVT Z990003). After 4 h ...
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No products found
because this supplier's products are not listed.
Aske L. Ejdrup, et al.,
bioRxiv - Cell Biology 2022
Quote:
... anti-hNET at 1:1000 (MAb Technologies, NET17-1) and anti-EEA1 at 1:1000 (Abcam ...
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No products found
because this supplier's products are not listed.
Takao Fujisawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Recombinant proteins or ZnCl2 solution for normalization was incubated with 10 µM ZnAF-2 (Goryo Chemical, SK2001-01) in TBS for 30 min at room temperature ...
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No products found
because this supplier's products are not listed.
Eun Jeong Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Plasma ACTH concentrations were measured using the ACTH ELISA kit (MD Biosciences), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Renata Varnaitė, et al.,
bioRxiv - Immunology 2020
Quote:
... SARS-CoV-2 specific IgM antibodies were detected using EDI Novel Coronavirus COVID-19 IgM ELISA kit (Epitope Diagnostics), according to the manufacturer’s instructions ...
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PRG-1 (EDTA -dPBS Solution) prepares the cells for PRG-2 (containing Trypsin) processing. Cell...
Cat# 4Z0-610,
100.0 mL, $68.0
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Africa Fernandez-Nasarre, et al.,
bioRxiv - Immunology 2023
Quote:
... Keratinocytes were cultured in an incubator at 5% CO2 at 35°C in 6-well plates (Falcon) coated with PureCol bovine collagen I solution (Cell Systems) in low calcium homemade culture medium containing recombinant mEGF (Peprotech ...
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No products found
because this supplier's products are not listed.
Kelly M. Hennessey, et al.,
bioRxiv - Cell Biology 2020
Quote:
... the C terminus of GL50803_8445 was tagged with an 11-amino acid flexible linker and the fluorescent protein mNeonGreen (Allele Biotechnology). The mNG-N11-Neo vector was constructed for this purpose by amplifying mNeonGreen using the primers listed in Table S1 ...
-
No products found
because this supplier's products are not listed.
Leticia R. Q. Souza, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the primary antibodies [Non-structural protein 1 from ZIKV (NS1, 1:500, BioFront Technologies, BF1225-06;), Class III β-tubulin (TuJ3 ...
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No products found
because this supplier's products are not listed.
Kevin M. Knox, et al.,
bioRxiv - Neuroscience 2021
Quote:
... FluoroJade-C (FJ-C; catalog #1FJC) was from Histo-Chem Inc (Jefferson ...
-
No products found
because this supplier's products are not listed.
Mayra Martinez-Lopez, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Mitomycin C(0.5mg/mL; Medac)/L-PBS or Mitomycin C/L-Clodronate were injected intratumorally and xenografts were placed immediately in clean E3 medium ...
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No products found
because this supplier's products are not listed.
Alyssa F. Pybus, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were incubated at 4°C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200 ...
-
No products found
because this supplier's products are not listed.
Kimberley El, et al.,
bioRxiv - Cell Biology 2023
Quote:
... levels were normalized to total protein levels (Sigma-Aldrich, #E3138, 1:1000 with Nacalai USA Signal Enhancer HIKARI), and total (i.e ...
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No products found
because this supplier's products are not listed.
Galit H. Frydman, et al.,
bioRxiv - Immunology 2019
Quote:
... 10 µL of cells were then imaged on a disposable C-Chip hemocytometer (In Cyto, SKC, Inc. C-Chip) using a 10X and 20X objective on a Nikon TiE fluorescent microscope ...
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No products found
because this supplier's products are not listed.
Rhett A. Snyder, et al.,
bioRxiv - Microbiology 2019
Quote:
... Quantification of c-di-GMP in sample extracts was determined using a standard curve generated from chemically synthesized c-di-GMP (AXXORA). The standard curve solutions were prepared using twofold serial dilutions of c-di-GMP (1.25 μM – 19 nM ...
-
No products found
because this supplier's products are not listed.
Stephan Brouwer, et al.,
bioRxiv - Microbiology 2020
Quote:
... The primary antibodies used for the detection of SpeC and SSA protein in GAS culture supernatants were rabbit antibody to SpeC (PCI333, Toxin Technology; 1:1,000 dilution) and affinity-purified rabbit antibody to SSA (produced by Mimotopes ...
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No products found
because this supplier's products are not listed.
Shalini Gupta, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The N- and C-peptides were labeled with maleimide-derivatized DY549P1 (Dyomics), DY649P1 (Dyomics) ...
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No products found
because this supplier's products are not listed.
Lindsay Smith, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... zebrafish were incubated for 5 min at 28.5°C with optovin 6b8 (ID 5705191l; ChemBridge), an optovin analog (Kokel et al. ...
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No products found
because this supplier's products are not listed.
Clay D. Jackson-Litteken, et al.,
bioRxiv - Microbiology 2022
Quote:
... Purified protein was then used by Antibody Research Corporation (St. Peters, MO) to generate polyclonal rabbit antibody.
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No products found
because this supplier's products are not listed.
Konstantinos Nakos, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Cells were cultured in 24 well plates on 12 mm round glass coverslips (Bellco Glass, Inc.), coated with 1 mg/mL poly-L-Lysine (OKK-3056 ...
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No products found
because this supplier's products are not listed.
Stijn De Munter, et al.,
bioRxiv - Immunology 2023
Quote:
... 10 μM Rho-associated coiled–coil containing protein kinase (ROCK) inhibitor Y-27632 (Abmole) and 5 μM A83-01 (Tocris Bioscience)) ...
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No products found
because this supplier's products are not listed.
Kei Sugihara, et al.,
bioRxiv - Cell Biology 2020
Quote:
... we used red fluorescent protein (RFP)-labeled HUVECs and GFP-labeled pericytes from Angio-proteomie Inc ...
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No products found
because this supplier's products are not listed.
Blake L. Cooper, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Cells were maintained under standard cell culture conditions (37°C, 5% CO2) in iCell cardiomyocyte maintenance media (Fujifilm Cellular Dynamics) supplemented with 1 mg/mL ciprofloxacin (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Saki Kiuchi, et al.,
bioRxiv - Biochemistry 2024
Quote:
RAW 264.7 cells were grown at 37 °C in DMEM (Dulbecco’s modified Eagle medium) supplemented with 10% EquaFETAL (Atlas Biologicals, Inc.) in a humidified 5% CO2 atmosphere ...
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No products found
because this supplier's products are not listed.
Nicole Fazio, et al.,
bioRxiv - Biophysics 2023
Quote:
... ε260 = 8700 M− 1cm−1 for TMP) and ε260 = 4930 M−1 cm−1 for Cy3 and ε260 = 10000 M−1 cm−1 for Cy5 (Glen Research) based on the sequence of each DNA strand (Supplementary Table S1) ...
-
No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... three-fold dilutions of VHH-Fcs were prepared in V- Bottom 96-well microtiter plates (Globe Scientific, Mahwah, NJ, Cat# 120130) and mixed with 50 µL of CHO-SPK cells ...
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No products found
because this supplier's products are not listed.
Xiaoquan Zhu, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and concentrated using ViraTrap lentivirus purification kit (Biomiga). NIH3T3 or RWPE-1 cells or PC3 or LNCaP at 90% confluence were infected with Lenti-FOXP2 ...
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No products found
because this supplier's products are not listed.
Kevin Zheng, et al.,
bioRxiv - Biochemistry 2020
Quote:
... plates were read on a Berthold Mithras LB940 using pre-warmed media and instrument at 37 °C using a standard RLuc emissions filter (480 nm) with a custom mKO 542 nm long-pass emission filter (Chroma Technology Co., Bellows Falls, VT) as previously described (Smith and Pack et al. ...
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No products found
because this supplier's products are not listed.
C Kimberly Tsui, et al.,
bioRxiv - Cell Biology 2023
Quote:
... CSA (EY Laboratories, BA-3201-1), and SLBR-H and SLBR-N (made in-house in the Mahal Lab).
-
No products found
because this supplier's products are not listed.
Aviad Ben-Shmuel, et al.,
bioRxiv - Immunology 2021
Quote:
... Rabbit anti-pSHP-1 (S591) (ECM Biosciences), Rabbit anti-pPLCγ1 (Y783 ...
-
No products found
because this supplier's products are not listed.
Mohammed Samer Shaban, et al.,
bioRxiv - Immunology 2020
Quote:
... and Dylight488-conjugated (ImmunoReagents #DkxMu-003D488NHSX, 1:100) secondary antibodies were used ...
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No products found
because this supplier's products are not listed.
Sepiedeh Keshavarzi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... submerged in 1 % Tergazyme (in distilled water, Alconox) for at least an hour ...