-
No products found
because this supplier's products are not listed.
Maria Körner, et al.,
bioRxiv - Cell Biology 2023
Quote:
Pulldown assays using immobilized GST fusion proteins or biotinylated peptide (Biotin- CQGLYFHINQTLREAHFHSLQHRG-COOH; PANATecs GmbH, Tübingen, Germany) were essentially performed as described (Böhm et al ...
-
No products found
because this supplier's products are not listed.
Zahra Hosseinzadeh, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The insulin of each sample was measured with the C-Peptide & Insulin AccuBind VAST ELISA Kits (Monobind Inc., USA). Three independent experiments were performed for each group ...
-
No products found
because this supplier's products are not listed.
Alyssa F. Pybus, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were incubated at 4°C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200 ...
-
No products found
because this supplier's products are not listed.
Charlotta Hansson, et al.,
bioRxiv - Immunology 2023
Quote:
EAE was induced in 8-9 weeks old C57BL/6 mice or SJL/J mice after an intradermal injection with 100µg myelin oligodendrocyte glycoprotein (MOG35-55) or proteolipid protein (PLP139-151) peptide (MD Biosciences) emulsified in Complete Freund’s Adjuvant (CFA ...
-
No products found
because this supplier's products are not listed.
Tyson J. Ruetz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Slides were treated with 50-70 µL blocking solution (5% normal donkey serum [NDS, ImmunoReagents, SP-072-V×10] ...
-
No products found
because this supplier's products are not listed.
Pavlo Gilchuk, et al.,
bioRxiv - Immunology 2020
Quote:
... mice were treated with 2 mg of an Ifnar1-blocking antibody (MAR1-5A3, Leinco Technologies) by i.p ...
-
No products found
because this supplier's products are not listed.
Andrew V. Grassetti, Rufus Hards, Scott A. Gerber,
bioRxiv - Cell Biology 2021
Quote:
... lyophilized peptides were dissolved in 50% acetonitrile (ACN; Honeywell) / 2M lactic acid (Lee Biosolutions), incubated with 1.25 mg TiO2 microspheres (GL Sciences ...
-
No products found
because this supplier's products are not listed.
Sarath Vijayakumar, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Protein kinases predicted to act on phosphorylation sites within the array peptide sequences were identified using GPS 3.0 and Kinexus Phosphonet (Kinexus Bioinformatics) (102–104) ...
-
Protein Assay Kit (QCPR-500)
Cat# QCPR-500,
1.0 kit, 500 tests, USD $199.0
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Anne L. Rosen, et al.,
bioRxiv - Immunology 2023
Quote:
... Total protein in the urine was measured using Quantichrom Total Protein Assay Kit (CAS: QTPR-100, BioAssay Systems) according to the manufacturer’s instructions with samples detected at a 1:4-1:20 dilution ...
-
No products found
because this supplier's products are not listed.
Ekaterina Kozaeva, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... rapidly frozen by plunging into slushed liquid nitrogen (N2) at −210°C and transferred to the preparation chamber stage at −180°C (PP2000 CryoTransferTM System; Quorum Technologies, Laughton, East Sussex, United Kingdom). Frozen samples were cleaved with a cold knife (facilitating an exposed surface in the fractured sample) ...
-
No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
-
No products found
because this supplier's products are not listed.
Gabriela Zurawska, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mice received i.v.solution of liposomes containing clodronic acid (LIPOSOMA, #C-SUV-005) (5 ml/kg ...
-
No products found
because this supplier's products are not listed.
Andrew I. Wong, et al.,
bioRxiv - Microbiology 2022
Quote:
... samples were separated on a Cogent Diamond Hydride Type C column (Microsolv Technologies). The mobile phase consisted of solvent A (ddH2O with 0.2% formic acid ...
-
No products found
because this supplier's products are not listed.
Garth Blackler, et al.,
bioRxiv - Physiology 2023
Quote:
... overnight at 4°C and decalcified in Decal Stat (StatLab, PC-1212-1) for 24 hours ...
-
No products found
because this supplier's products are not listed.
Kelsey E. Zuk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The tissue was superfused with oxygenated ACSF at 35°C via a peristaltic pump (Gilson) at a rate of 3-3.5 ml/min.
-
No products found
because this supplier's products are not listed.
Josiane Reist, et al.,
bioRxiv - Microbiology 2019
Quote:
... influenzae isolates were frozen at −70 °C in cryogenic MicrobankTM vials (Pro-Lab Diagnostics, Birkenhead, UK). Prior to testing ...
-
No products found
because this supplier's products are not listed.
E Hsu, NR Zemke, AJ Berk,
bioRxiv - Molecular Biology 2020
Quote:
... were grown at 37°C in a BronchiaLife medium complete kit (LL-0023; Lifeline Cell Technology) in a 5% CO2 incubator until they reached confluence ...
-
No products found
because this supplier's products are not listed.
Benoît P. Nicolet, et al.,
bioRxiv - Immunology 2019
Quote:
... were pre-coated overnight at 4°C with 4μg/mL rat α-mouse IgG2a (MW1483, Sanquin) in PBS ...
-
No products found
because this supplier's products are not listed.
Maxwell C. Coyle, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... Genotyping PCRs were performed in 96-well plates (Brooks Life Sciences Cat. No. 4ti-0770/c) using Q5 polymerase (NEB Cat ...
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No products found
because this supplier's products are not listed.
Katy Pilarzyk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibody was applied overnight at 4°C for PDE11A (Fabgennix PD11-101 at 1:500). The following day ...
-
No products found
because this supplier's products are not listed.
Kellie A. Cotter, et al.,
bioRxiv - Genomics 2022
Quote:
... to reduce uncapped RNAs to 5′ hydroxyls and make them incapable of ligating to 5′ adaptor and Cap-Clip (catalog no. C-CC15011H; Cambio) to remove the 5′ cap of transcripts that had undergone guanylation and allow them to be incorporated into the library through 5′ adapter ligation ...
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No products found
Yuriko Tachida, et al.,
bioRxiv - Neuroscience 2022
Quote:
Human brain microvascular endothelial cells (BMECs, Applied Cell Biology Research Institute) were cultured in CS-C complete medium (Cell Systems) with FBS and used within four passages ...
-
No products found
because this supplier's products are not listed.
Saki Kiuchi, et al.,
bioRxiv - Biochemistry 2024
Quote:
RAW 264.7 cells were grown at 37 °C in DMEM (Dulbecco’s modified Eagle medium) supplemented with 10% EquaFETAL (Atlas Biologicals, Inc.) in a humidified 5% CO2 atmosphere ...
-
No products found
because this supplier's products are not listed.
Wakana Sato, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... at 37°C for 1 hour and agarose gel-purified with GenCatch Advanced Gel Extraction Kit (Epoch Life Science, Inc., No. 2260050). eGFP fluorescence was measured at λex 488 nm and λem 509 nm with plate reader PMT setting “medium” and 6 reads per well ...
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No products found
because this supplier's products are not listed.
László Imre, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cyclodextrin/peptide complex formation was performed by mixing 30 μM peptide and 300 μM SBECD (Sulfobutylether-β-Cyclodextrin; CycloLab, Budapest, Hungary) diluted in colorless ...
-
No products found
because this supplier's products are not listed.
Hui Zhang, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Samples were boiled at 100 °C in a metal bath for 10 min in protein loading buffer (CoWin Biosciences, Beijing, China) and equal amount proteins were separated by 10% SDS-PAGE gel ...
-
The peptide is used to block Anti-C-Reactive Protein Antibody (#CPA1275) reactivity.
Cat# CBP1275,
1 mg USD $100.0, 5 mg USD $300.0
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Rafał Zdrzałek, et al.,
bioRxiv - Plant Biology 2024
Quote:
... membranes were incubated with appropriate antibodies diluted in blocking buffer (α-FLAG: Cohesion Biosciences, at 1:3000 dilution ...
-
No products found
because this supplier's products are not listed.
Milena Petkova, et al.,
bioRxiv - Cell Biology 2022
Quote:
Mouse Vascular Endothelial Cell Growth Factor C (VEGF-C) ELISA Kit from CUSABIO (CSB-E07361m) was used for detection of VEGF-C protein concentration ...
-
No products found
because this supplier's products are not listed.
Sylvain Perriot, et al.,
bioRxiv - Immunology 2024
Quote:
... -C (1:500, AffinityImmuno, clone W6/32) were incubated in 250ul blocking buffer with neurons overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Cheng Wu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Microglass pipettes filled with 1 μl Aβ-555 peptide solution were connected to a microinjection system (BASi, USA). Aβ40-555 were used among hypertension groups and Aβ42 555 were used among APP/PS1 groups ...
-
No products found
because this supplier's products are not listed.
Dennis Alejandro Escolástico-Ortiz, et al.,
bioRxiv - Microbiology 2023
Quote:
... followed by 1 h at 45°C and 2 h at 65°C in a heating block digestion system (DigiPREP, SCP Sciences). After digestion ...
-
No products found
because this supplier's products are not listed.
Colton D. Payne, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The resin used as an anchor for peptide assembly was Tentagel XV 4-hydroxymethyl phenoxyacetic acid (Rapp Polymere, GmbH). Prior to the loading of the C-terminal residue ...
-
No products found
because this supplier's products are not listed.
Nikola Cousin, et al.,
bioRxiv - Immunology 2021
Quote:
OT-1 effector T cells were generated by ex vivo culture of total Ly5.1+ OT-1 splenocytes in T cell medium supplemented with 1 ng/ml SIINFEKL peptide and 100 U/ml recombinant mouse IL-2 (ImmunoTools) for 72 h ...
-
No products found
because this supplier's products are not listed.
Eric Blanc, et al.,
bioRxiv - Genomics 2019
Quote:
For immunisation 8-12-week old ABabDII mice were injected subcutaneously with 100 µg of mutant short peptide (9-10mers, JPT) supplemented with 50 µg CpG 1826 (TIB Molbiol), emulsified in incomplete Freund’s adjuvant (Sigma) ...
-
No products found
because this supplier's products are not listed.
Li-Juan Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and PRRSV nucleocapsid protein (rabbit monoclonal, VMRD) antibodies were used to localize JEV ...
-
No products found
because this supplier's products are not listed.
Jonathan M Budzik, et al.,
bioRxiv - Microbiology 2019
Quote:
Immunostaining for LC3 was performed with blocking and permeabilization buffer (0.3% Triton X-100, 2% bovine serum albumin) and anti-LC3 (clone 2G6, Nanotools #0260-100/LC3-2G6) at a dilution of 1:200 for 3 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Kevin Christian M. Gulay, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Endogenous peroxidases were quenched with 0.3% H2O2 in methanol for 15 min at RT before blocking the tissue sections with 10% normal rabbit serum (Nichirei biosciences, Tokyo, Japan) for an hour at RT and incubating with KDM2B antibody (sc-293279 ...
-
No products found
because this supplier's products are not listed.
Bruno Raposo, et al.,
bioRxiv - Immunology 2022
Quote:
... All clones were initially identified as ACPAs by screening using antigen microarray binding to citrullinated peptides and CCP2 by CCPlus ELISA (Svar Life Science) at 5 μg/ml ...
-
No products found
because this supplier's products are not listed.
Janneke G.C. Peeters, et al.,
bioRxiv - Immunology 2024
Quote:
... and 200 μg MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK, Genemed Synthesis) and received intraperitoneal injections of 200 ng Pertussis Toxin from Bordetella pertussis (List biological Laboratories) at the time of immunization and 48h later ...
-
No products found
because this supplier's products are not listed.
Niamh E. Harrington, et al.,
bioRxiv - Microbiology 2019
Quote:
... Tissue was incubated at 37 °C with a Breathe-Easier® membrane (Diversified Biotech) for the desired length of time.
-
No products found
because this supplier's products are not listed.
Clay D. Jackson-Litteken, et al.,
bioRxiv - Microbiology 2022
Quote:
... Purified protein was then used by Antibody Research Corporation (St. Peters, MO) to generate polyclonal rabbit antibody.
-
Cat# SC22000,
Coupling buffer+ Washing buffer + EDC, USD $76.00/KIT
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
-
No products found
because this supplier's products are not listed.
Chance J. Cosgriff, et al.,
bioRxiv - Microbiology 2019
Quote:
... Precipitated proteins were washed with 1 mL of 100% ethanol (Decon Laboratories Inc.) and incubated at 4°C for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Suchitra Kamle, et al.,
bioRxiv - Immunology 2022
Quote:
... The pseudovirus with omicron S protein was obtained from eEnzyme (Gaithersburg, MD, USA). Pseudoviruses containing S protein mutations of COVID variants used in this study can be seen in Supplementary Table S1 ...
-
No products found
because this supplier's products are not listed.
Jimi L. Rosenkrantz, et al.,
bioRxiv - Developmental Biology 2021
Quote:
BeWo cells were cultured at 37°C 5% CO2 in Ham’s F-12 (Kaighn’s Modification) media (Caisson Labs, HFL06-500ML) supplemented with 10% FBS (Sigma ...
-
No products found
because this supplier's products are not listed.
Soo-Yeon Kang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HLOs were cleared by incubating in 30 μL of 1x pre-warmed (at 37°C) RapiClear 1.52 (Sunjin Lab, Taiwan) or Visikol® Histo-M™ (SKU ...
-
No products found
because this supplier's products are not listed.
Shanshan He, et al.,
bioRxiv - Genomics 2022
Quote:
The breast cancer biopsy used for protein assay was CTRL301 tissue microarray supplied by US Biomax, Inc ...
-
No products found
because this supplier's products are not listed.
An Phu Tran Nguyen, et al.,
bioRxiv - Neuroscience 2019
Quote:
... sections were pre-incubated in 4% PFA (in 0.1 M PB) for ≥2 days at 4°C before processing with the FD NeuroSilver™ kit II (FD Neurotechnologies, Inc.) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Mobashar Hussain Urf Turabe Fazil, et al.,
bioRxiv - Immunology 2021
Quote:
Real-time trans-well migration of T-cells was monitored at 37°C using the CIM-Plate 16 and xCELLigence impedance-based detection system (ACEA biosciences, Singapore) to evaluate transmigration of T-cells towards the chemoattractant SDF-1α (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Abigail A. Mornement, et al.,
bioRxiv - Physiology 2023
Quote:
... Lysate was incubated for 1.5 hours at 55 °C then transferred to screw cap vials containing 0.4 g sterilised low binding 100-micron silica beads (OPS Diagnostics, BLBG 100-200-11) and vortexed for 10 minutes at max speed ...