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No products found
because this supplier's products are not listed.
Hari-Hara SK Potula, et al.,
bioRxiv - Microbiology 2019
Quote:
Borrelia burgdorferi sensu stricto low passage strain B31 was (obtained from the American Type Culture Collection Manassas, VA) used for MIC tests and all infection studies in C3H/HeN mice ...
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No products found
because this supplier's products are not listed.
Andrew C. Krusenstjerna, et al.,
bioRxiv - Microbiology 2022
Quote:
... burgdorferi B31 genomic DNA with Taq polymerase and TA cloned into pCR2.1 (Thermo Fisher). Constructs were sequenced to guarantee sequence fidelity.
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No products found
because this supplier's products are not listed.
Emily Petroni, et al.,
bioRxiv - Microbiology 2023
Quote:
... burgdorferi B31 (PA003) spirochetes were diluted into BSK-II or BSK-II +10mM spermidine (Sigma Aldrich, Catalog #S2626-1G) adjusted to pH 7.8 ...
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No products found
because this supplier's products are not listed.
SS Madhavan, et al.,
bioRxiv - Physiology 2023
Quote:
... 2 µL of protein ladder (BIORAD 1610374) was used ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi B31-e2 expression strains using the DNeasy Blood and Tissue Kit (Qiagen). The open reading frame (lacking the putative lipoprotein signal sequence ...
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No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi antibody (Abcam, ab20950) was added to two tubes ...
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No products found
because this supplier's products are not listed.
Nuno M. Gonçalves, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Protein quantification was performed with 2-D Quant kit (GE Healthcare) following manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Bryan T. Murphy, et al.,
bioRxiv - Microbiology 2022
Quote:
... burgdorferi cells (62) using QuickLoad 2x Taq Master Mix (NEB, M0271) and primers 49 and 50 (Table 1) ...
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No products found
because this supplier's products are not listed.
Mariarosa Mezzanotte, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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No products found
because this supplier's products are not listed.
Ling Zhang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Yongjun Sui, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg/ml of SARS-CoV-2 S1 protein (Sino Biological) for PBMC ...
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No products found
because this supplier's products are not listed.
Jingru Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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No products found
because this supplier's products are not listed.
Sanchita Mishra, et al.,
bioRxiv - Immunology 2024
Quote:
... anti-SARS-CoV-2 N protein (Cell Signaling Technology, 33717). HRP-conjugated secondary antibodies used in this study were Jackson Immuno Research Laboratories anti-rabbit (111-035-047 ...
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No products found
because this supplier's products are not listed.
Clotilde Garrido, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... resuspended in protein resuspension buffer (2% SDS, 10 mM NaF, 1x Roche cOmplete Mini proteinase inhibitor cocktail ...
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No products found
because this supplier's products are not listed.
William K. Boyle, et al.,
bioRxiv - Microbiology 2020
Quote:
... burgdorferi dksA was commercially synthesized (GenScript, Piscataway, NJ, United States). The dksA gene was PCR amplified using primers listed in supplemental table 1 and cloned into the NheI site of the pMAL-C5X plasmid expression vector using a Gibson assembly kit (New England Biosciences ...
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No products found
because this supplier's products are not listed.
Naomi-Liza Denning, et al.,
bioRxiv - Immunology 2019
Quote:
... and macrophage inflammatory protein-2 (MIP-2) (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Yan Gao, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Plasmids for SARS-CoV-2 structural proteins were purchased from Addgene (Appendix Table 2). Lentiviral supernatant was collected according to the manual using 293FT cells ...
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No products found
because this supplier's products are not listed.
Claire Yung, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and 2) cytosolic protein markers: anti-TSG101 (mouse, BD Biosciences ...
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No products found
because this supplier's products are not listed.
Monica Delgado-Vergas, et al.,
bioRxiv - Cell Biology 2020
Quote:
... We immunoprecipitated proteins with mouse anti Bcl-2 (C-2) (sc-7382 from Santa Cruz), rabbit anti Beclin1 (ab55878 from Abcam ...
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No products found
because this supplier's products are not listed.
Camila H. Coelho, et al.,
bioRxiv - Immunology 2023
Quote:
... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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No products found
because this supplier's products are not listed.
Xin Liu, et al.,
bioRxiv - Bioengineering 2021
Quote:
Primary human umbilical vein endothelial cells (HUVECs) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were maintained in EMG-2 medium (complete EGM-2 BulletKitTM, Lonza). We obtained human umbilical cords from Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine ...
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No products found
because this supplier's products are not listed.
Zheng Ruan, et al.,
bioRxiv - Biophysics 2021
Quote:
... The solubilized protein was incubated with 2 mL TALON cobalt metal-affinity resin (Takara Bio) for 1 h ...
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No products found
because this supplier's products are not listed.
Natalia G. Herrera, et al.,
bioRxiv - Biochemistry 2020
Quote:
Recombinant SARS-CoV-2 Spike protein was coated onto high-binding 96-well plates (Corning, 3690) at 2 μg/mL ...
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No products found
because this supplier's products are not listed.
Satoru N. Kinoshita, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 2 (Illumina) were used for construction of complementary DNA libraries and the complementary DNA libraries were sequenced on a NextSeq 500 system (Illumina) ...
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No products found
because this supplier's products are not listed.
Zaw Min Htet, et al.,
bioRxiv - Biochemistry 2019
Quote:
... in 2 mL Protein Lo Bind Tubes (Eppendorf) using the following protocol ...
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No products found
because this supplier's products are not listed.
Yubao Fan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 10 ng/ml bone morphogenetic protein-2 (BMP2; Peprotech), 1% penicillin-streptomycin solution (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Steven Z. Chou, Thomas D. Pollard,
bioRxiv - Biochemistry 2023
Quote:
... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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No products found
because this supplier's products are not listed.
Ashley M Ngo, et al.,
bioRxiv - Microbiology 2019
Quote:
... Dengue virus Type 2 NS5 protein (Genetex, GTX629447) and GFP (Genetex ...
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No products found
because this supplier's products are not listed.
Daniela Bottero, et al.,
bioRxiv - Immunology 2023
Quote:
Spike proteins (2 μg/dose) were formulated with Alhydrogel 2% (InvivoGen, San Diego, CA, USA) as previously described (20) ...
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No products found
because this supplier's products are not listed.
Bryce W. Duncan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... coiled-coil containing protein kinase (ROCK1/2) Y27632 (Tocris, Inc.) at 10 µM in PBS for 1-2 hr ...
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No products found
because this supplier's products are not listed.
Kuan-Yi Lu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
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No products found
because this supplier's products are not listed.
Vasiliki-Ilya Gargareta, et al.,
bioRxiv - Neuroscience 2022
Quote:
... protein-arginine deiminase 2 (PADI2, Proteintech #12110-1-AP, 1:1000), tetraspanin-29 (CD9 ...
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No products found
because this supplier's products are not listed.
Ziva Vuckovic, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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No products found
because this supplier's products are not listed.
Noam Zuela-Sopilniak, et al.,
bioRxiv - Cell Biology 2020
Quote:
Total protein levels were calibrated using Bicinchoninic Acid (BCA) assay (ThermoFisher Pierce BCA Protein Assay kit, OD measured with BioTek synergy 2 multi-mode multiplate reader). Samples were ran on a 9% SDS polyacrylamide gel ...
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No products found
because this supplier's products are not listed.
Kylene M. Harold, et al.,
bioRxiv - Physiology 2023
Quote:
... Total protein was stained (Revert Total Protein Stain, LI-COR) and imaged (Odyssey CLx ...
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No products found
because this supplier's products are not listed.
Edward Sullivan, et al.,
bioRxiv - Microbiology 2021
Quote:
... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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No products found
because this supplier's products are not listed.
M. Verónica López, et al.,
bioRxiv - Immunology 2021
Quote:
... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
-
No products found
because this supplier's products are not listed.
Steven Z. Chou, Thomas D. Pollard,
bioRxiv - Biochemistry 2023
Quote:
... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
-
No products found
because this supplier's products are not listed.
Tian Yu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... non-specific protein binding was blocked in a medium consisting of 2% normal donkey serum (Jackson ImmunoResearch) in antibody medium (AB medium ...
-
No products found
because this supplier's products are not listed.
Murphy LY Wan, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
-
No products found
because this supplier's products are not listed.
Takafumi Nishida, et al.,
bioRxiv - Physiology 2023
Quote:
... anti-rabbit anti-sterol regulatory element-binding protein-2 (SREBP2, Cayman chemical, MI), anti PCSK9 (Cayman chemical) ...
-
No products found
because this supplier's products are not listed.
Jianan Zhang, et al.,
bioRxiv - Biophysics 2024
Quote:
... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Braulio Cardenas-Benitez, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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No products found
because this supplier's products are not listed.
Kaare Bjerregaard-Andersen, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
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No products found
because this supplier's products are not listed.
Annika Ullrich, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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No products found
because this supplier's products are not listed.
Sara C.M. Leijon, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 2 software (Olympus) and digitized/stabilized using FIJI (ImageJ) ...
-
No products found
because this supplier's products are not listed.
Christian Hartmann, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Zen 2 (Blue Edition, Carl Zeiss) and Imaris (Bitplane ...
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No products found
because this supplier's products are not listed.
Tomas C. Pascoa, et al.,
bioRxiv - Biochemistry 2023
Quote:
... prior to denaturing intact protein mass spectrometry analysis the protein (2 mg mL-1) was incubated with 200 µM sphinganine (Avanti Polar Lipids), 200 µM fumonisin B1 (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Anne-Sophie Fiorucci, et al.,
bioRxiv - Plant Biology 2019
Quote:
... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...
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Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...
Cat# LS004174,
100 mg, $42.00
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Naoto Soya, et al.,
bioRxiv - Biophysics 2023
Quote:
... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...