-
No products found
because this supplier's products are not listed.
Chunyu Shang, et al.,
bioRxiv - Plant Biology 2024
Quote:
... The maltose binding protein label protein purification kit was used to further purify the obtained MBP-SlHDA1 fusion protein (Abbkine (Wuhan) Biotechnology Co. ...
-
No products found
because this supplier's products are not listed.
Iosifina P. Foskolou, et al.,
bioRxiv - Immunology 2022
Quote:
... Eu-Protein A (5 nM, Cisbio), Streptavidin-Alexa Fluor 647 (6.25 nM ...
-
No products found
because this supplier's products are not listed.
Ana Lucia Rosales Rosas, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Primary antibody anti-E2 protein [Chk265] (Absolute Antibody, Wilton, UK) was diluted in PBS-T (1:500 ...
-
No products found
because this supplier's products are not listed.
Jan Stuchly, et al.,
bioRxiv - Immunology 2024
Quote:
... Carrier-protein-free moAbs were purchased from Exbio (Prague, Czech Republic), BioLegend (San Diego ...
-
No products found
because this supplier's products are not listed.
Paweł P. Knejski, et al.,
bioRxiv - Biochemistry 2023
Quote:
... purified proteins were diluted to 0.05 mg/mL and applied onto plasma-cleaned (Gatan Solarus) copper grids with a continuous carbon layer (Electron Microscopy Sciences) ...
-
No products found
because this supplier's products are not listed.
Jonas L. Ravn, et al.,
bioRxiv - Microbiology 2022
Quote:
... were inoculated separately with a starting OD600= 0.1 or in different ratios (1:1 and 1:10) in Delft medium + 2% beechwood glucuronoxylan (Megazyme, Ireland) for 120 h at RT ...
-
No products found
because this supplier's products are not listed.
Morgan E. Hernandez Scudder, et al.,
bioRxiv - Neuroscience 2020
Quote:
A 1 m x 1 m three-chambered apparatus (Stoelting, Wood Dale, IL) was used as the testing arena (Bell et al. ...
-
No products found
because this supplier's products are not listed.
Sk. Kayum Alam, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... were incubated with 1 μg commercially available human IKKα protein (SignalChem) for 30 minutes at 30°C ...
-
No products found
because this supplier's products are not listed.
Li Dai, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1% w/v 800 nm Protein G-coated polystyrene beads (Spherotech Inc) were washed in the same way as the streptavidin beads ...
-
No products found
because this supplier's products are not listed.
Vitor Mendes, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was mixed in 1:1 and 1:2 (protein to reservoir) ratio with well solution using a mosquito robot (TTP labtech). Initial conditions were obtained in the Classics lite crystallization screen (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Ugo Dionne, et al.,
bioRxiv - Systems Biology 2020
Quote:
... Protein production was then induced by the addition of 1 mM IPTG (Bio Basic) and incubation at 16°C overnight with agitation ...
-
No products found
because this supplier's products are not listed.
Jing Shao, et al.,
bioRxiv - Physiology 2022
Quote:
... Protein concentration was determined using BCA Protein Assay Kit (TIANGEN) and diluted in loading dye (β-mercaptoethanol 5% ...
-
No products found
because this supplier's products are not listed.
Daria A. Egorova, et al.,
bioRxiv - Microbiology 2022
Quote:
... Total protein quantity was measured with QuDye Protein kit (Lumiprobe) on Qubit fluorometer (ThermoScientific).
-
No products found
because this supplier's products are not listed.
Sebastian Pöhl, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein was then purified using zinc-affinity chromatography using a 1 mL Zn-NTA column (Cube Biotech) equilibrated with lysis buffer containing 5 mM imidazole ...
-
No products found
because this supplier's products are not listed.
Ji-Hoon Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
... incubated with primary antibody against spike protein diluted 1:5,000 in blocking buffer (Elabscience, E-AB-V1006) overnight in a 37°C incubator with 5% CO2 injection ...
-
No products found
because this supplier's products are not listed.
Celia Fernandez-Sanz, et al.,
bioRxiv - Physiology 2021
Quote:
Equal amounts of protein (70 μg) supplemented with 5x Protein Loading Buffer (National Diagnostics, USA) were preheated (95°C ...
-
No products found
because this supplier's products are not listed.
Surya Cayre, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The following primary antibodies were used on mouse cell-derived lysates: anti-mouse milk proteins (Accurate Chemical; YNRMMSP; 1/2000), anti-mouse b-casein (a kind gift from C ...
-
No products found
because this supplier's products are not listed.
Francois Chesnais, et al.,
bioRxiv - Bioengineering 2022
Quote:
... a co-culture of 7.5×103 Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) and 1×105 Normal Human Dermal Fibroblasts (NHDF, Promocell) or Human Mesenchymal Stem cells (MSC ...
-
No products found
because this supplier's products are not listed.
Xiuling Gu, et al.,
bioRxiv - Immunology 2022
Quote:
... Biotinylation of A33 protein was performed by using the biotin-protein ligase kit (GeneCopoeia™, B1001) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Yifang Liu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Recombinant spike protein (BPS bioscience #100810) was captured by ACE2 receptor protein (Acro biosystems #AC2-H82F9 ...
-
No products found
because this supplier's products are not listed.
Joy H. Meserve, et al.,
bioRxiv - Neuroscience 2020
Quote:
... gRNA was combined with Cas9 protein (PacBio) and injected into one-cell stage embryos ...
-
No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
-
No products found
Daniel M. Sapozhnikov, Moshe Szyf,
bioRxiv - Molecular Biology 2020
Quote:
... and dCas9 recombinant protein (Applied Biological Materials Inc.). After 10 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Jens O. Watzlawik, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Non-phosphorylated Ub protein (Boston Biochem, U-100H), free non-phosphorylated ...
-
No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
-
No products found
because this supplier's products are not listed.
Silvia De Cicco, et al.,
bioRxiv - Neuroscience 2020
Quote:
... plus bone morphogenetic protein 4 (BMP4, 50 ng/mL, Immunotools), vascular endothelial growth factor (VEGF ...
-
No products found
because this supplier's products are not listed.
Brogan Ashley, et al.,
bioRxiv - Physiology 2021
Quote:
... or 2 μM Receptor Associated Protein (RAP; Innovative Research, USA), which block pinocytosis ...
-
No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Liver proteins were extracted by physical dissociation (Ultra-turrax, IKA) in PEB Buffer (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Anna Bludau, et al.,
bioRxiv - Neuroscience 2020
Quote:
... nuclear proteins were extracted using the EpiQuick Nuclear Extraction Kit (Epigentek) according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Shane Miersch, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
Fifty micrograms of protein were injected onto a TSKgel BioAssist G3SWxl (Tosoh) fitted with a guard column using an NGC chromatography system and a C96 autosampler (Biorad) ...
-
No products found
because this supplier's products are not listed.
Dhanu Gupta, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 2 µl of protein A conjugated 10 nm gold nanoparticles (BBI Solutions) were added and incubated for 45 minutes.
-
No products found
because this supplier's products are not listed.
Bevin C. English, et al.,
bioRxiv - Immunology 2022
Quote:
... proteins were extracted from samples collected in TRI Reagent (Molecular Research Center) according to a modified protocol (65) ...
-
No products found
because this supplier's products are not listed.
Richard Samuel Edward Babicz, et al.,
bioRxiv - Physiology 2022
Quote:
... Protein phosphorylation was visualized using autoradiography (Hyblot ES, Thomas Scientific, Swedesboro, NJ).
-
No products found
because this supplier's products are not listed.
Bryan D. Ryder, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the protein solution was loaded into 3.5kDa cutoff Biotech CE Dialysis Tubing (Spectrum Labs) and dialyzed overnight at 4°C in 1xPBS to restore native folding ...
-
No products found
because this supplier's products are not listed.
Carmanah Hunter, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Polysialylated proteins were eluted by incubating twice with 200 μL 25 mg/mL colominic acid (Carbosynth) at room temperature for 1 – 2 h and collecting the supernatant ...
-
No products found
because this supplier's products are not listed.
Suel-Kee Kim, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NANOG (AF1997, R&D, 1:200; Reprocell 1:200), OCT4A (MAB17591 ...
-
No products found
because this supplier's products are not listed.
Iris Lindberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... proSAAS (AAV 2/6; Vigene Biosciences) or enhanced green fluorescent protein (GFP; AAV 2/6; Vector Biolabs) was driven by the human SYN1 promoter ...
-
No products found
because this supplier's products are not listed.
Marie E Jönsson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 mM DTT) using a 1 ml tissue douncer (Wheaton). The homogenate was carefully layered on top of a sucrose cushion (1.8 M sucrose ...
-
No products found
because this supplier's products are not listed.
Alexandre P. Martin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... S6 (effect of ERM proteins on Notch2 signaling) were acquired using a Prime 95B Scientific CMOS camera (Photometrics), giving different signal arbitrary units from the ones recorded with the QuantEMTM 512SC CCD camera used in the other experiments ...
-
No products found
because this supplier's products are not listed.
Marijke I. Zonneveld, et al.,
bioRxiv - Immunology 2020
Quote:
... and 1 µg/ml soluble αCD28 (CLB-CD28/1, 15E8 Sanquin) in the presence or absence of milk EVs or EV depleted control ...
-
No products found
because this supplier's products are not listed.
Julia Böhme, et al.,
bioRxiv - Immunology 2020
Quote:
The activity of caspase-1 was determined using fluorescent inhibitor of caspase-1 (FAM-Flica Caspase-1 Assay kit, ImmunoChemistry Technologies). Staining procedure was performed according to manufactures protocol ...
-
No products found
because this supplier's products are not listed.
Maria Florencia Ercoli, et al.,
bioRxiv - Plant Biology 2022
Quote:
... 1% sucrose (pH 5.8) in 0.3% Gellex (Gellan Gum CAS#71010-52-1 Caisson Laboratories) supplemented with or without 1µM of the selected kinase inhibitor (see Supplemental Table S5 for a description of the compounds tested in this study) ...
-
No products found
because this supplier's products are not listed.
Daniela Saderi, Brad N. Buran, Stephen V. David,
bioRxiv - Neuroscience 2019
Quote:
... 1 to 4 high-impedance tungsten microelectrodes (FHC or A-M Systems, impedance 1-5 MΩ) were slowly advanced into cortex with independent motorized microdrives (Alpha-Omega) ...
-
No products found
because this supplier's products are not listed.
Danya Abazari, et al.,
bioRxiv - Neuroscience 2022
Quote:
Protein palmitoylation assay was performed using CAPTUREome S-palmitoylated protein kit (Badrilla, Leeds, UK), as described by manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Rahul Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
... T7-RILP proteins were expressed in Escherichia coli BL21 (500 μM isopropyl β-d-1-thiogalactopyranoside; Wisent Bioproducts; at room temperature for 16 hours) and purified using standard procedure in tris buffer [20 mM tris (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
Sheung Chun Ng, Dirk Görlich,
bioRxiv - Biophysics 2022
Quote:
... purified protein was dissolved in 2 M guanidine hydrochloride and the protein was allowed to react with Atto488 maleimide (ATTO-TEC, Germany) at 1:1 molar ratio at pH 6.8 ...
-
No products found
because this supplier's products are not listed.
George Cameron, et al.,
bioRxiv - Biophysics 2022
Quote:
... Anti-GINS antibody was affinity purified using protein A-sepharose (Covalab). For bulk replication assays ...
-
No products found
because this supplier's products are not listed.
Numan Ibne Asad, et al.,
bioRxiv - Microbiology 2022
Quote:
Our goal was to model grain quality (protein, gluten, BEM and PMT) using the microbial indicators measured (bacterial and fungal alpha diversity ...
-
No products found
because this supplier's products are not listed.
Eric A. Kirk, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Anesthesia was induced with isoflurane (1-5%, Kent Scientific), eye lubricant was applied ...
-
No products found
because this supplier's products are not listed.
James T. McKenna, et al.,
bioRxiv - Neuroscience 2020
Quote:
Mice were deeply anesthetized with isoflurane (1-3%) and viral injections were performed using a 1 µl Hamilton syringe (Cat#7001KH, Point Style 3, Hamilton Company, Reno, NV, USA), targeting BF (AP +0.14 mm ...