-
No products found
because this supplier's products are not listed.
Diana N. Medina-Pérez, et al.,
bioRxiv - Microbiology 2020
Quote:
... B burgdorferi strains were grown in BSK-II media supplemented with 6% normal rabbit serum (Pel-Freez Biologicals, Rogers, AR) under conventional microaerobic conditions at 32°C ...
-
No products found
because this supplier's products are not listed.
Thekla Cordes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The cell suspension was centrifuged at 20,000 g for 10 min at 4 °C and 20 µl of the supernatant was used for protein quantification using BCA protein assay kit (Cat. #G1002, Lamda Biotech. Inc) as per manufacturer’s instruction.
-
No products found
because this supplier's products are not listed.
Lorena P. Samentar, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Thioflavin T (ThT Chem-Impex #22870) staining was done to verify the presence of Aβ plaques in the brain of APP/PS1 AD mouse ...
-
No products found
because this supplier's products are not listed.
Mia P. Sams, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Samples were denatured at 100°C and separated on 10% SDS-PAGE gels with BLUeye Prestained Protein Ladder (FroggaBio, Concord ON). Protein was then semi-dry transferred to a PVDF membrane and incubated in Li-Cor Intercept® Blocking Buffer (Cedarlane ...
-
No products found
because this supplier's products are not listed.
Zachary Ingram, et al.,
bioRxiv - Microbiology 2024
Quote:
... Primary CD4+ T cells were isolated from whole blood (BioIVT) using the CD4+ T Cell Isolation Kit (Miltenyi Biotec ...
-
No products found
because this supplier's products are not listed.
Yuxin Lin, et al.,
bioRxiv - Biophysics 2024
Quote:
... supplemented with 10% tetracycline-free FBS (Neuromics, no. FBS002-T) and 1 × penicillin-streptomycin (Corning ...
-
No products found
because this supplier's products are not listed.
Kevin M. Knox, et al.,
bioRxiv - Neuroscience 2021
Quote:
... FluoroJade-C (FJ-C; catalog #1FJC) was from Histo-Chem Inc (Jefferson ...
-
No products found
because this supplier's products are not listed.
Franziska Blaeschke, et al.,
bioRxiv - Immunology 2022
Quote:
... T cells were incubated with NY-ESO-1 specific dextramer (Immudex) for 12 min at RT (1:50 dilution) ...
-
No products found
because this supplier's products are not listed.
Zhijie Yang, et al.,
bioRxiv - Microbiology 2023
Quote:
... iranensis was constructed using pESAC13-A from Bio S&T (Montreal, Canada). Based on a high-throughput screening method (unpublished) ...
-
No products found
because this supplier's products are not listed.
John Joseph, et al.,
bioRxiv - Bioengineering 2023
Quote:
Madin-Darby canine kidney cells (ATCC®) were cultured in T-175 flasks (CELLTREAT) at 37°C and 5% CO2 in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Tyler B. Waltz, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant rat protein p11 (S100A10 Recombinant Protein, Aviva Systems Biology, OPCD06771) was dissolved in distilled water to obtain a final concentration of 100ug/mL and stored at -80C for less than one month ...
-
No products found
because this supplier's products are not listed.
Natalia Gebara, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and 2% Chang Medium C (Irvine Scientific), 20% Fetal Calf Serum (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Washington C. Agostinho, Paulo E. Brandão,
bioRxiv - Molecular Biology 2019
Quote:
FITC-antibody control protein (Gelantis) complexed with Bioporter® Protein Delivery Reagent (Genlantis) per manufacturer’s instructions were injected by the intracerebral route in two mice and the CNS of each mouse was collected at 4 and 6 hours post-infections ...
-
No products found
because this supplier's products are not listed.
Luisa de Lemos, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Fluoro-Jade C labelling was performed on retinal organoids cryosections using the Fluoro-Jade® C staining kit (Biosensis) and following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Danya Abazari, et al.,
bioRxiv - Neuroscience 2022
Quote:
Protein palmitoylation assay was performed using CAPTUREome S-palmitoylated protein kit (Badrilla, Leeds, UK), as described by manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Sonu Kumar, et al.,
bioRxiv - Microbiology 2020
Quote:
The SEC-purified Fab M4H2K1 and complex were each concentrated to 12 mg/ml before being screened at both 4 °C and 20 °C using our high-throughput CrystalMationTM robotic system (Rigaku) at TSRI (103) ...
-
No products found
because this supplier's products are not listed.
Domokos I. Lauko, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and in adherent culture on T-25 tissue culture flasks in Grace’s media (Gemini Bio-Products; Expression systems) with 10% FBS (Gemini Bio-Products ...
-
No products found
because this supplier's products are not listed.
Mohan Kumar Muthu Karuppan, et al.,
bioRxiv - Immunology 2019
Quote:
... Viral proteins were purchased from ImmunoDx, Woburn ...
-
No products found
because this supplier's products are not listed.
Xiufang Kong, Amr H Sawalha,
bioRxiv - Genetics 2019
Quote:
... on the Omega Lum C imaging system (Gel Company).
-
No products found
because this supplier's products are not listed.
Saejeong Park, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... fixed in warm 4% paraformaldehyde (Thomas Scientific, 37 °C) for 5 min and washed with PBS 3 times ...
-
No products found
because this supplier's products are not listed.
Kenneth Johnson, et al.,
bioRxiv - Microbiology 2022
Quote:
... at 37°C in flow chambers (IBI scientific, UK). The system was sterilized by pumping a 0.2 % of hypochlorite solution for 1 hour using a peristaltic pump ...
-
No products found
because this supplier's products are not listed.
Satish Rojekar, et al.,
bioRxiv - Bioengineering 2023
Quote:
... or at 37 °C in a water bath (PolyScience), and analyzed by dynamic light scattering or protein thermal shift assay ...
-
No products found
because this supplier's products are not listed.
Danielle L. Michell, et al.,
bioRxiv - Immunology 2019
Quote:
... total RNA was isolated from splenic CD4+ T cells (isolated MACS) using the Total RNA Isolation kits (Norgen Biotek). Total RNA sequencing (RNA-seq ...
-
No products found
because this supplier's products are not listed.
Junichi Suzuki,
bioRxiv - Physiology 2020
Quote:
... Total protein concentrations were measured using PRO-MEASURE protein measurement solution (iNtRON Biotechnology Inc., Gyeonggi-do, Korea).
-
No products found
because this supplier's products are not listed.
Aman Y. Husbands, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Membranes were washed three times with PBS-T and incubated with 1:2000 anti-His-tag antibody (Abiocode M0335-1) for 1h with gentle shaking at RT ...
-
No products found
because this supplier's products are not listed.
Donna Ye, et al.,
bioRxiv - Microbiology 2019
Quote:
... Media supplemented with milk protein (Hardy Diagnostics) was made by first sterilizing 2x media stocks (TSB or R2B ...
-
No products found
because this supplier's products are not listed.
Li-Ying Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Recombinant MANF protein (P-101-100, Icosagen) in PBS at 200 ng/ul was used as positive control ...
-
No products found
because this supplier's products are not listed.
Artyom Luzhin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and Cas9 3NLS protein (IDT DNA Technologies) was added to make functional ribonucleoprotein (RNP) ...
-
No products found
because this supplier's products are not listed.
Rebecca O’Cleirigh, Roslyn Gibbs,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and incubated at 37°C and 5% CO2 (Nuaire, DH Autoflow). Media was changed every 48 hours and cells passaged when they reached confluence as recommended by the supplier ...
-
No products found
because this supplier's products are not listed.
Yuki Muroyama, et al.,
bioRxiv - Immunology 2022
Quote:
... HiFi SpCas9 protein (SpyFiTM) was purchased commercially (Aldevron) and aliquoted to avoid frequent freeze/thawing.
-
No products found
because this supplier's products are not listed.
Alexis Vivoli, et al.,
bioRxiv - Cell Biology 2021
Quote:
... islets were washed once with D-PBS + 2 mM EDTA solution (339xg, 3 min, 4°C) and digested by enzymatic disaggregation for 10 min at 37°C using Accutase (Innovative Cell Technologies Inc., San Diego, CA, USA). The reaction was stopped using islet culture medium and the islet cell suspension was washed once with PBS and dead cells labeled using the LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (405 nm ...
-
No products found
because this supplier's products are not listed.
Aliya Sharipova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... the Fe with 1wt% VH precursor mixture was loaded into a custom-build consolidation die and high-pressure cold-sintered at 2.5 GPa (corresponding to 5 t for 5 mm diameter die) and RT using manual press (Carver, Wabash, IN, USA) to obtain the drug-loaded metal (Supplementary Materials ...
-
No products found
because this supplier's products are not listed.
Emanuel J. Novais, et al.,
bioRxiv - Cell Biology 2024
Quote:
... deparaffinized sections were blocked in 5% normal serum in PBS-T and incubated with antibodies against COL2 (1:400, Fitzgerald 70R-CR008), COMP (1:200 ...
-
No products found
because this supplier's products are not listed.
Ah-Lai Law, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Protein A bead precleared lysates were incubated with primary antibody or non-immune control IgG followed by 1% BSA blocked protein A beads (Pierce) or protein A/G beads (Alpha Diagnostics). For GFP-trap or Myc-trap pulldowns ...
-
No products found
because this supplier's products are not listed.
Yu-Yi Kuo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The supernatants were collected and the protein concentration was quantified by the Bradford protein assay (BioShop Canada Inc., Burlington, Ontario, Canada). Protein samples were stored at −80°C for subsequent western blotting assessments.
-
No products found
because this supplier's products are not listed.
Linshan Sun, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Primary antibodies: c-Fos (CST, #2250) and tyrosine hydroxylase (TH) (ImmunoStar, #22941). The numbers of c-Fos ...
-
No products found
because this supplier's products are not listed.
Adam B. Yasunaga, Isaac T.S. Li,
bioRxiv - Biophysics 2021
Quote:
Protein G-coated polystyrene beads (Spherotech, PGP-60-5) were functionalized with PSGL1-Fc Chimera (R&D systems ...
-
No products found
because this supplier's products are not listed.
Reza Nouri, et al.,
bioRxiv - Bioengineering 2022
Quote:
... LwaCas13a proteins were purchased from MCLAB (cat# CAS13a-100). Cas13a and crRNA were mixed in 1×PBS to form the non-activated Cas13a/crRNA at room temperature for 20 min and stored at -80°C ...
-
No products found
because this supplier's products are not listed.
Mackenzie E. Ryan, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein sequence alignments were performed using MegAlign Pro (DNAstar). Superimposed images of protein structures were generated using ChimeraX Matchmaker84 ...
-
No products found
because this supplier's products are not listed.
Shao-Jung Hsu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... one given adeno-associated virus (AAV)8-mouseVEGF-C (Vector Biolabs, Malvern, PA) and the other given AAV8-GFP (control ...
-
No products found
because this supplier's products are not listed.
Deborah L. Gater, et al.,
bioRxiv - Biophysics 2022
Quote:
... Vitamin D binding protein (DBP) was purchased from Athens Research and Vitamin D Binding protein (VDR ...
-
No products found
because this supplier's products are not listed.
Yosif Zaki, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Layers of adhesive cement (C&B Metabond) followed by dental cement (A-M Systems) were spread over the surgical site ...
-
No products found
because this supplier's products are not listed.
Mastura Akter, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Stainless steel guide cannulae (Double/O.D.0.41mm-27G/C, cat # 62069, RWD life science.com.ltd.) were bilaterally positioned into ACC region (2.4 mm anterior to bregma and 0.5 mm lateral from midline ...
-
No products found
because this supplier's products are not listed.
Anna Bludau, et al.,
bioRxiv - Neuroscience 2020
Quote:
... nuclear proteins were extracted using the EpiQuick Nuclear Extraction Kit (Epigentek) according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Yuxin Lin, et al.,
bioRxiv - Biophysics 2024
Quote:
... The sample was then positioned onto a heated stage (37 °C. Heating elements: Bioscience Tools, TC-E35 ...
-
No products found
because this supplier's products are not listed.
Evelyn Ploetz, et al.,
bioRxiv - Biophysics 2020
Quote:
... The radio-labelled compounds [3 H]-asparagine and [14 C]-glutamine were obtained from American Radiolabeled Chemicals and PerkinEllmer ...
-
No products found
because this supplier's products are not listed.
Panga Jaipal. Reddy, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Analytical column (PICOCHIP: 105 cm, 1.9 µm, REPROSIL Pur C-18-AQ, 120 Å, New Objective, USA) with a flow rate of 300 nL/min was used for the separation of the peptides with a linear gradient of 5–35% buffer-B (90% acetonitrile in 0.1% FA ...
-
No products found
because this supplier's products are not listed.
Catherine A. Cotter, et al.,
bioRxiv - Microbiology 2022
Quote:
... wells were washed three times with 250 μl PBS + 0.05% Tween-20 (PBS-T, Accurate Chemical). Plates were blocked for 2 h at room temperature with 200 μl PBS-T + 5% nonfat milk and subsequently washed three times with PBS-T prior to incubation with a series of eight 4-fold dilutions of mouse sera for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Alexander Sheh, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cultures were incubated at 37° C in an anaerobic chamber (Coy Lab Products) with mixed gas (10% CO2 ...
-
No products found
because this supplier's products are not listed.
Carl-Fredrik Bowin, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Venus fluorescence was measured first and subsequently 10 µl of Coelenterazine h (Biosynth C-7004) (2.5 µM final concentration ...