-
No products found
because this supplier's products are not listed.
Heidi Peussa, et al.,
bioRxiv - Bioengineering 2022
Quote:
... transmitted light (T-PMT) was used to focus the stimulation laser to the DR1-glass layer and defined regions of interest (3 x 5 rectangles á 7×400 pixels ...
-
No products found
because this supplier's products are not listed.
P. Soblechero-Martín, et al.,
bioRxiv - Neuroscience 2021
Quote:
... aiming to skip DMD exon 51 (51-[T*C*A*A*G*G*A*A*G*A*T*G*G*C*A*T*T*T*C*T]-3⍰, Eurogentec, Belgium) by transfection with Lipofectamine as described in43,44 and analysed by either myoblot (96 well plates ...
-
No products found
because this supplier's products are not listed.
Malvina Schatz, et al.,
bioRxiv - Microbiology 2022
Quote:
... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
-
No products found
because this supplier's products are not listed.
D Kaplun, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... PCR product was cloned to t-vector (pAL2-T, Evrogen, Russia) and sequenced by Sanger for at least 10 clones for each point.
-
No products found
because this supplier's products are not listed.
J. Christopher Rounds, et al.,
bioRxiv - Neuroscience 2021
Quote:
... brains were left rocking at 4°C for 1-3 nights in 0.1% PBS-T supplemented with blocking agent normal goat serum (Jackson ImmunoResearch) at a 1:20 dilution ...
-
No products found
because this supplier's products are not listed.
Tea Dodig-Crnković, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
-
No products found
because this supplier's products are not listed.
Jacob G. Smith, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Primary antibodies were diluted in 5% milk TBS-T and incubated with blots at 4°C overnight (BMAL1, Abcam – ab93806; ACTIN, Abcam – ab3280, P84, Genetex – GTX70220). Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate ...
-
No products found
because this supplier's products are not listed.
Elisa Jentho, et al.,
bioRxiv - Immunology 2019
Quote:
... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
-
No products found
because this supplier's products are not listed.
Maria Font Farre, et al.,
bioRxiv - Plant Biology 2023
Quote:
... membranes were blocked in TBS-T/milk 5% overnight at 4°C and incubated in 1:2000 anti-PR2 (Agrisera, AS122366) in TBS-T/milk 5% for 1.5h at room temperature ...
-
No products found
because this supplier's products are not listed.
Isaree Teriyapirom, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or 50μM T-5224 (Tocris) until the end of the experiment ...
-
No products found
because this supplier's products are not listed.
Mahboubeh Yazdanifar, et al.,
bioRxiv - Cancer Biology 2019
Quote:
1×106 CAR T cells expressing CAR-mKate were plated in 35 mm Poly-d-lysine Coated MatTek dish (MatTek #P35GCOL-0-14-C) for 24 hrs ...
-
No products found
because this supplier's products are not listed.
Wren E. Michaels, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Quantitative analysis of the CFTR B and C-bands was performed using Compass software (Protein Simple).
-
No products found
because this supplier's products are not listed.
Jinkai Zang, et al.,
bioRxiv - Microbiology 2020
Quote:
... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
-
No products found
because this supplier's products are not listed.
David Y. Nie, et al.,
bioRxiv - Biochemistry 2023
Quote:
... C-terminally biotinylated SKP1-FBXO22 fusion proteins (WT or C326A) were immobilized onto SA biosensors (Sartorius) to a response level of 1 nm ...
-
No products found
because this supplier's products are not listed.
Tiphaine M.N. Camarasa, et al.,
bioRxiv - Microbiology 2023
Quote:
... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
-
No products found
because this supplier's products are not listed.
Rachel P. Wallace, et al.,
bioRxiv - Bioengineering 2023
Quote:
... For the immunogenic protein challenge experiments 9 week old female Balb/c mice were purchased from Charles River Laboratory.
-
No products found
because this supplier's products are not listed.
Panga Jaipal. Reddy, et al.,
bioRxiv - Biochemistry 2023
Quote:
Log phase B31 and MM1 Borrelia samples were analyzed using 5600+ Triple-TOF mass spectrometry (ABSciex, USA) coupled with Eksigent 400 nano-HPLC (Sciex, USA). Peptides from the 2 isolates were run separately by loading on trap column (200 μm × 0.5 mm ...
-
No products found
because this supplier's products are not listed.
Simon Gemble, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were washed three times using PBS-T (PBS 1X + 0,1% Triton X-100 + 0,02% Sodium Azide) and incubated with PBS-T + BSA (04-100-812-C from Euromedex) 1% for 30 min at RT ...
-
No products found
because this supplier's products are not listed.
Sarah Araten, et al.,
bioRxiv - Genetics 2023
Quote:
... Isolated nuclei were first stained for 40 minutes at 4°C with Cardiac Troponin T (Novus Biological NB120-10214AF488) at a 1:100 dilution in 0.01% BSA ...
-
No products found
because this supplier's products are not listed.
Franziska Jäger, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
No products found
because this supplier's products are not listed.
Tyler N. Starr, et al.,
bioRxiv - Microbiology 2022
Quote:
... Proteins were biotinylated overnight at 4°C using a BirA biotinprotein ligase reaction kit (Avidity) and re-purified using the same HisTrap HP affinity method described above before being flash frozen.
-
No products found
because this supplier's products are not listed.
Alex J.B. Kreutzberger, et al.,
bioRxiv - Microbiology 2021
Quote:
... TRIS (Goldbio, cat. T-400-500), EDTA (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Michael J. McFadden, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Thioflavin T (Cayman Chemical, 5 µM) was added 2 hours prior to endpoint ...
-
No products found
because this supplier's products are not listed.
Wai-Lok Yau, et al.,
bioRxiv - Cell Biology 2024
Quote:
... PI4KB inhibitor T-00127-HEV1 (Calbiochem # 538001 ...
-
No products found
because this supplier's products are not listed.
P. C. Steketee, et al.,
bioRxiv - Microbiology 2021
Quote:
... UK). Libraries were prepared from 8 samples (4× T. brucei, 4× T. congolense) using the TruSeq Stranded mRNA kit (Illumina) and 2 × 75 bp paired-end sequencing was carried out using a HiSeq 4000 system (Illumina) ...
-
No products found
because this supplier's products are not listed.
David A. Russo, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Nostoc was grown in T-75 tissue flasks (Sarstedt) with 20 mL of BG-11 TES medium ...
-
No products found
because this supplier's products are not listed.
Edwin B. Corgiat, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... then washed 5x in PBS-T and mounted in Vectashield (Vector Labs). Antibodies used ...
-
No products found
because this supplier's products are not listed.
Chun Su, et al.,
bioRxiv - Genomics 2019
Quote:
... pGFP-C-shRNA-Lenti-B2M and pGFP-C-scrambled were purchased from Origene. The packaging vectors PmD2G and PsPAX.2 were obtained from Addgene ...
-
No products found
because this supplier's products are not listed.
Ryan J. Garrigues, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi cells were incubated in normal human serum (NHS; Complement Technology) at a final concentration of 15% ...
-
No products found
because this supplier's products are not listed.
Melissa J. Mann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
-
No products found
because this supplier's products are not listed.
Margrethe A. Boyd, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... Protein expression was monitored at 37°C in a SpectraMax i3x plate reader (Molecular Devices). GFP was monitored at ex ...
-
No products found
because this supplier's products are not listed.
Zhihao Zhang, et al.,
bioRxiv - Immunology 2022
Quote:
Spleens from immunized animals were removed at Day 51 for the mice and at Day 110 for the hamsters and splenocytes were isolated for protein S-specific T-cell detection with the ELISpotPLUS mouse IFN-γ or ELISpotPLUS hamster IFN-γ kits (Mabtech) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Tanja Bhuiyan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and ectopic protein expression in Flp-In T-REx HeLa cells was induced with 1 μg/mL doxycycline (MP Biomedicals, 195044, Dox) minimum three hours post-seeding ...
-
No products found
because this supplier's products are not listed.
Chih-Jen Wei, et al.,
bioRxiv - Immunology 2022
Quote:
... in PBS-T (BioVision #2310-100). Serial dilutions of serum were made in 2.5% milk and 0.5% BSA in PBS-T ...
-
No products found
because this supplier's products are not listed.
Anne Kühnel, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the following markers were measured with enzyme-linked immunosorbent assay (ELISA): high-sensitivity C-reactive protein (Tecan Group Ltd. ...
-
No products found
because this supplier's products are not listed.
Xi Mou, Shiau Wei Liew, Chun Kit Kwok,
bioRxiv - Biochemistry 2021
Quote:
... Thioflavin T (ThT) was ordered from Solarbio Life Science ...
-
No products found
because this supplier's products are not listed.
Gabriele Ciceri, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Josephine W. Thinwa, et al.,
bioRxiv - Microbiology 2022
Quote:
... guinea pig anti-p62/SQSTM1 (C-terminus) (Progen, GP62-C), mouse anti-SQSTM1 (Abnova ...
-
No products found
because this supplier's products are not listed.
Amine Driouchi, et al.,
bioRxiv - Biophysics 2023
Quote:
... ribonucleoprotein (RNP) complexes included sgRNAs targeting either N- or C-terminal region (Integrated DNA Technologies - IDT) and Cas9 protein (PNA bio, Cat #CP01) were transfected together with linear dsDNA donors (IDT ...
-
No products found
because this supplier's products are not listed.
Kishio Furuya, et al.,
bioRxiv - Physiology 2021
Quote:
... using a confocal X-Z-T scan (LSM510, Carl Zeiss, Oberkochen, Germany) with a 63× NA1.4 objective ...
-
No products found
because this supplier's products are not listed.
Marion Thépaut, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins were detected using InstantBlue protein stain (Expedeon) according to the supplier’s instructions.
-
No products found
because this supplier's products are not listed.
Stefano Cattaneo, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and incubated with primary antibodies diluted in blocking solution (anti-SST 1:100 v/v, Millipore, and anti-red fluorescent protein 1:400 v/v, Rockland Immunochemicals, overnight at 4°C). Slices were then washed for 1h by alternating PBS and blocking solution every 15 min ...
-
No products found
because this supplier's products are not listed.
Mayumi K. Holly, et al.,
bioRxiv - Microbiology 2020
Quote:
... C-reactive protein (MyBioSource). The optical density was read at 450 nm on an EnVision plate reader (PerkinElmer) ...
-
No products found
because this supplier's products are not listed.
Jing Yang (John) Wang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 100 mM glycine to glow-discharged C-flat CF-2/2 C-T-grids (TED PELLA).
-
LC Laboratories' Product Number D-3197 - Dorsomorphin, Free Base (AMPK Inhibitor I, BML-275, BMP...
Cat# D-3197, SKU# D-3197_10mg,
10 mg, $57.00
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Hui-Hsuan Kuo, et al.,
bioRxiv - Cell Biology 2019
Quote:
... thiazovivin (LC Labs, T-9753), recombinant human TGFβ3 (Cell Guidance Systems ...
-
No products found
because this supplier's products are not listed.
Kim T Melville, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein fluorescence was measured at 25 °C on a CLARIOstar multimode plate reader (BMG Labtech), with excitation at 295 ± 10 nm ...
-
Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Cat# LS001643,
5x1 mg, $166.00
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Annika Müller-Eigner, et al.,
bioRxiv - Physiology 2021
Quote:
... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
-
No products found
because this supplier's products are not listed.
Taro Maeda, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... ground with a T-10 basic homogenizer (IKA, Germany) to a fine powder ...
-
No products found
because this supplier's products are not listed.
Soumen Barman, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Protein expression was induced overnight at 17°C with 0.5 mM IPTG in an Innova 44 Incubator shaker (New Brunswick Scientific). Proteins were purified as follows ...
-
No products found
because this supplier's products are not listed.
S.A. Stern, et al.,
bioRxiv - Neuroscience 2019
Quote:
... washed in PBS-T and mounted in Vectamount with DAPI (Southern Biotech). Antibodies used here were ...