-
No products found
because this supplier's products are not listed.
Yi-Pin Lin, et al.,
bioRxiv - Microbiology 2020
Quote:
... Borrelia burgdorferi genomic equivalents were calculated using an Applied Biosystems 7500 Real-Time PCR system (ThermoFisher Scientific) in conjunction with PowerUp SYBR Green Master Mix (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Michael J. Pereira, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi antibody (Abcam, ab20950) was added to two tubes ...
-
No products found
because this supplier's products are not listed.
Xiaotian Tang, et al.,
bioRxiv - Microbiology 2021
Quote:
... burgdorferi N40 were grown at 33°C in Barbour-Stoenner-Kelly H (BSK-H) complete medium (Sigma-Aldrich, #B8291) with 6% rabbit serum ...
-
No products found
because this supplier's products are not listed.
Hari-Hara SK Potula, et al.,
bioRxiv - Microbiology 2019
Quote:
Borrelia burgdorferi sensu stricto low passage strain B31 was (obtained from the American Type Culture Collection Manassas, VA) used for MIC tests and all infection studies in C3H/HeN mice ...
-
No products found
because this supplier's products are not listed.
William K. Boyle, et al.,
bioRxiv - Microbiology 2020
Quote:
... burgdorferi dksA was commercially synthesized (GenScript, Piscataway, NJ, United States). The dksA gene was PCR amplified using primers listed in supplemental table 1 and cloned into the NheI site of the pMAL-C5X plasmid expression vector using a Gibson assembly kit (New England Biosciences ...
-
No products found
because this supplier's products are not listed.
Jack M. Botting, et al.,
bioRxiv - Microbiology 2022
Quote:
... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
-
No products found
because this supplier's products are not listed.
Sandhya Singh, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Precision Plus Protein Western C ladder (BioRad) and Precision protein Streptactin-HRP conjugate (BioRad ...
-
No products found
because this supplier's products are not listed.
Bryan T. Murphy, et al.,
bioRxiv - Microbiology 2022
Quote:
... burgdorferi cells (62) using QuickLoad 2x Taq Master Mix (NEB, M0271) and primers 49 and 50 (Table 1) ...
-
No products found
because this supplier's products are not listed.
Eun Na Kim, et al.,
bioRxiv - Pathology 2020
Quote:
C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
-
No products found
because this supplier's products are not listed.
Jun-Jie Zhang, et al.,
bioRxiv - Microbiology 2022
Quote:
... burgdorferi cultures (around 108 cells) using the RNeasy Mini kit (Qiagen). DNA contamination in the RNA samples was removed by DNase I (New England Biolabs ...
-
No products found
because this supplier's products are not listed.
K. Ehring, et al.,
bioRxiv - Cell Biology 2023
Quote:
... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
-
No products found
because this supplier's products are not listed.
Bernd M. Mitic, et al.,
bioRxiv - Microbiology 2022
Quote:
... 55 mmol L-1 NaCl (Merck KGaA, pH 8.2; T = −27 °C). The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm ...
-
No products found
because this supplier's products are not listed.
Ali Hosseini Rad SM, et al.,
bioRxiv - Immunology 2020
Quote:
CAR T cells were stained with biotin anti-c-myc antibody (Biolegend #908805) detected with Streptavidin-Brilliant Violet 421 (Biolegend #405225) ...
-
No products found
because this supplier's products are not listed.
Jared Hollinger, et al.,
bioRxiv - Biochemistry 2023
Quote:
Two vectors with C-terminal MBP (maltose-binding protein)-His6 Tag (2Cc-T; Addgene Plasmid #55209) or N-terminal His10-MBP Tag (2CT-10 ...
-
No products found
because this supplier's products are not listed.
Matilda Toivakka, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-T-bet and anti-c-Myc (Cell Signaling) antibodies ...
-
No products found
because this supplier's products are not listed.
Ci Zhu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... CD8+ T cells were fixed 10 min at 37 °C with Cytofix (BD), permeabilized 20 min on ice with methanol and stained with the indicated phospho-Abs for 30 min in the dark at 4°C in PBS/2% FCS ...
-
No products found
because this supplier's products are not listed.
Sk. Kayum Alam, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... immunostained using a C-terminal antibody that recognize both DARPP-32 and t-DARPP (Santa Cruz Biotechnology ...
-
No products found
because this supplier's products are not listed.
Shipra Das, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
-
No products found
because this supplier's products are not listed.
Maike Sperk, et al.,
bioRxiv - Microbiology 2020
Quote:
... C-reactive protein (CRP, R&D systems), and CCL20 (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Catherine M. Porter, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and cultured at 37°C and 5% CO2 in T-175 flasks (Corning) in minimum essential medium (MEM ...
-
No products found
because this supplier's products are not listed.
Marta Varela-Eirín, et al.,
bioRxiv - Cell Biology 2019
Quote:
... in the T/C-28a2 chondrocyte cell after transfection with a pIRESpuro2 plasmid construct (Clontech) containing the human Cx43 sequence ...
-
No products found
because this supplier's products are not listed.
Marta Sanz, et al.,
bioRxiv - Microbiology 2023
Quote:
Phosphorylation status of p65 and c-JUN were analyzed by flow cytometry in isolated CD4 T cells (StemCell Technologies) as previously described (44) ...
-
No products found
because this supplier's products are not listed.
Douglas C. Palmer, et al.,
bioRxiv - Immunology 2020
Quote:
... T cells were measured at 37°C using an Xfe96 extracellular analyzer (Seahorse Bioscience). Briefly ...
-
No products found
because this supplier's products are not listed.
Nicholaus J. Trenton, et al.,
bioRxiv - Biophysics 2019
Quote:
... program T-024 and Amaxa Nucleofector Solution T (Lonza). Briefly ...
-
No products found
because this supplier's products are not listed.
Igor A. Gak, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Ribosomal protein L23/RPL23 rabbit polyclonal (Bethyl Laboratories, A305-008A-T, 1:1000), Ribosomal protein L26/RPL26 (D8F6 ...
-
No products found
because this supplier's products are not listed.
Vamseedhar Rayaprolu, et al.,
bioRxiv - Biophysics 2022
Quote:
C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
-
No products found
because this supplier's products are not listed.
Nako Okamoto, et al.,
bioRxiv - Microbiology 2022
Quote:
... Membranes were incubated overnight at 4°C with a primary antibody for SARS-CoV-2 (2019-nCoV) S protein (Sino Biological, 40589-T62; diluted 1:2,000 in PBS-T). Next ...
-
No products found
because this supplier's products are not listed.
Lester Frei, et al.,
bioRxiv - Immunology 2023
Quote:
... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
-
No products found
because this supplier's products are not listed.
Michela Winters, et al.,
bioRxiv - Microbiology 2021
Quote:
... Wells of the 12-well microtiter plate (VWR 734-2324 T C-treated 12-well plates) were prepared just before inoculation and imaging by coating the bottom of each well with 15 µl of Con A and allowing the wells to dry ...
-
No products found
because this supplier's products are not listed.
Luis-Alberto Perez-Quintero, et al.,
bioRxiv - Immunology 2023
Quote:
To evaluate protein phosphorylation in CD8 T cells after stimulation with IFN-β (Peprotech), day 4 CTL were washed twice in serum-free media and incubated for 2 hours under serum and cytokine deprivation before stimulation ...
-
No products found
because this supplier's products are not listed.
Wenjie Wang, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The target protein images were obtained with C-DiGit Blot scanner (LI-COR) and analyzed by ImageStudio (LI-COR) ...
-
No products found
because this supplier's products are not listed.
Veronika Thallmair, et al.,
bioRxiv - Cell Biology 2020
Quote:
... DS-Red-T (590-650 nm) and CFP-T (460-500 nm) emission filters (Leica). Images were acquired with an ORCA-Flash4.0 C13440-20C camera (Hamamatsu photonics ...
-
No products found
because this supplier's products are not listed.
Chun Su, et al.,
bioRxiv - Genomics 2019
Quote:
... prior to sorting naïve T cells (CD4+CD45RO-) and T follicular helper cells (CD4+CD45RO+CD25loCXCR5hiPD1hi) on a MoFlo Astrios EQ (Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Deepak K. Singh, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 0.5 × 106 T-HEp3-GFP cells were injected subcutaneously in 8-week old female BALB/c nu/nu mice (Jackson Laboratories). Mice were inspected regularly for arising tumors and when tumors reached ∼300 mm3 ...
-
No products found
because this supplier's products are not listed.
Daniel Levy, et al.,
bioRxiv - Bioengineering 2023
Quote:
... were cultured in T-75 tissue culture flasks coated with 0.1% gelatin using endothelial growth medium (C-C22121; PromoCell, Heidelberg, Germany). RAW264.7 mouse macrophages (T1B71 ...
-
No products found
because this supplier's products are not listed.
Brandon M Bensel, et al.,
bioRxiv - Biophysics 2023
Quote:
... Liposomes were extruded through a 1 μm pore diameter extruder (T&T Scientific) and incubated overnight with 1 μM thiolated neutravidin (SH-NaV) ...
-
No products found
because this supplier's products are not listed.
Karansher S. Sandhu, et al.,
bioRxiv - Genetics 2021
Quote:
... Grain protein content (%) and grain yield (t/ha) was collected using a Perten DA 700 NIR analyzer (Perkin Elmer, Sweden) and Wintersteiger Nursery Master combine (Ried im Innkreis ...
-
No products found
because this supplier's products are not listed.
Seraina A. Domenig, et al.,
bioRxiv - Cell Biology 2023
Quote:
... at 37°C using DirectPCR Lysis Reagent (mouse tail) (VIG102-T, Viagen Biotech). PCR for Pax7-nGFP was performed using primer Pax7nGFP.F/ Pax7nGFP.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
-
No products found
because this supplier's products are not listed.
Josephine L Tan, et al.,
bioRxiv - Biophysics 2021
Quote:
MR experiments were performed at 7 T (Bruker BioSpec 7 T). A home-built transmit/receive surface coil (46.1 MHz ...
-
No products found
because this supplier's products are not listed.
Bartosz Gabryelczyk, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
The experiments were performed at room temperature (T = 23 ± 1 °C)and visualized with an inverted microscope (Nikon Eclipse Ti) equipped with a 20x objective and associated with a 1.5x magnification device ...
-
No products found
because this supplier's products are not listed.
Goutam Dey, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... T-LCK (1:1000 Proteintech), P-LCK 394 (1:1000 R&D Systems) ...
-
No products found
because this supplier's products are not listed.
Gemma Gou, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Special chow (T.2019.12, Envigo) was administered to pregnant mothers and litter until weaning (postnatal day [PND] 21) ...
-
No products found
because this supplier's products are not listed.
Adam McNee, et al.,
bioRxiv - Immunology 2020
Quote:
... To deplete T cell populations BALB/c mice received one intraperitoneal injection of 500 µg of anti-mouse CD4 (BE0003-1, BioXCell, West Lebanon, NH) and 500 µg of anti-mouse CD8α (BioXCell ...
-
No products found
because this supplier's products are not listed.
C. Keating, et al.,
bioRxiv - Microbiology 2020
Quote:
... Slides were examined by light microscopy using an Olympus Vanox-T microscope and images were taken with a digital camera (Olympus camedia C-2020 Z).
-
No products found
because this supplier's products are not listed.
Selene García-Hernández, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
-
No products found
because this supplier's products are not listed.
Huafeng Wang, et al.,
bioRxiv - Physiology 2021
Quote:
... from adult male mice were transferred to the fibronectin-coated 37°C chamber for Delta T culture dish controller (Bioptechs) filled with HS and HEPES-buffered HTF medium (H-HTF)(Chung et al ...
-
No products found
because this supplier's products are not listed.
Angelique Peret, et al.,
bioRxiv - Neuroscience 2019
Quote:
... We confirmed that expression of the fosGFP transgene correlated with the synthesis of the endogenous c-fos protein using a c-fos specific antibody (Synaptic Systems GmbH ...
-
No products found
because this supplier's products are not listed.
Alok V. Joglekar, et al.,
bioRxiv - Immunology 2019
Quote:
... Blood was collected using plain hematocrit tubes (Drummond; 1-000-7500-C/50) into K2-EDTA tubes (Terumo CapiJect; T-MQK or Greiner; 450480) and processed the same day ...
-
No products found
because this supplier's products are not listed.
Alexandra A. Silverman, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and 1 μL/mL CellBrite Steady Membrane Dye (Biotium, 30105-T and 30108-T). Initially ...
-
No products found
because this supplier's products are not listed.
Catherine F. Ruff, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and somatostatin (Peninsula Laboratories, LLC; T-4547), followed by the corresponding Alexa Fluor-conjugated secondary antibodies (1:500 ...