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No products found
because this supplier's products are not listed.
Wanda M. Figueroa-Cuilan, et al.,
bioRxiv - Microbiology 2022
Quote:
... Hi-FBS was achieved by placing the FBS (Atlas Biologicals, FP-0500-A) in a water bath at ∼56 °C for 30 minutes ...
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No products found
because this supplier's products are not listed.
Kathleen M. Mulvaney, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the N-terminal tag was removed by incubation with HRV 3C protease (AG Scientific) and further purified by Superose 6 column ...
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No products found
because this supplier's products are not listed.
Mohd Farid Abdul Halim, et al.,
bioRxiv - Microbiology 2023
Quote:
... maripaludis MM1265 or Δfdh1 Δfdh2 expressing fdh2-His were grown in 1-liter anaerobic Schott bottles (Chemglass) containing McCas-formate medium with a N2:CO2 (80:20 ...
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No products found
because this supplier's products are not listed.
Dewi Safitri, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... HEK293T cells overexpressing full-length GIPR or GLP-1R with an N-terminal FLAG tag were purchased from Multispan, Inc (Hayward ...
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No products found
because this supplier's products are not listed.
Markus K. Klose, Junghun Kim, Edwin S. Levitan,
bioRxiv - Neuroscience 2023
Quote:
... A plasmid for UAS-Kir2.1 without a GFP tag provided by Sean Sweeney (University of York) was used by Bestgene Inc ...
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No products found
because this supplier's products are not listed.
Raveen Rathnasinghe, et al.,
bioRxiv - Microbiology 2021
Quote:
... were maintained in Dulbecco’s Modified Eagle Medium (DMEM) complemented with 10% heat-inactivated Fetal Bovine Serum (HI-FBS; PEAK serum), penicillin-streptomycin (Gibco ...
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No products found
because this supplier's products are not listed.
Cunxi Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... The Mpp75Aa1.1-His fractions identified by SDS-PAGE were pooled and further processed using EndoTrap® HD (Hyglos GmbH, Germany) to reduce endotoxin contents (manufacturer’s instruction) ...
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No products found
because this supplier's products are not listed.
Nirupa Nagaratnam, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The supernatant containing detergent solubilized His-tagged FopA was filtered through a 0.22 μm cellulose acetate membrane filter (Foxx Life Sciences) and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP ...
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No products found
because this supplier's products are not listed.
Sabina Marciano, et al.,
bioRxiv - Neuroscience 2021
Quote:
... primary antibody incubation was performed for 24 h at room temperature (VMAT2: 1:5000, 20042, Immunostar, Hudson, USA, HA-tag: 1:5000, MMS- 101R, Nordic BioSite, Taby ...
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No products found
because this supplier's products are not listed.
Gerda E. Breitwieser, et al.,
bioRxiv - Neuroscience 2023
Quote:
WT GPR37L1 and variants (without or with the Glu-Glu tag) were transfected into GPR37L1-KO SK cells (SK-N-MC Transfection Kit, Altogen Biosystems). Cells were lysed after 48 hours ...
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No products found
because this supplier's products are not listed.
Cassidy M.R. Blackburn, et al.,
bioRxiv - Immunology 2020
Quote:
... media was removed and replaced with either D-10 (untreated) or D-10 + 50μg/ml oxidized LDL (hi oxLDL Kalen biomedical 770252-60) for 2 hours ...
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No products found
because this supplier's products are not listed.
Vadim Malis, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and their subtraction provides flow-out signals from the tag pulse (Miyazaki et al., 2008; 2011; 2012; 2015; Wheaton et al., 2012). Both tag-on and tag-off acquisitions have the non-sel IR pulse and thus the background signals with exponential T1 relaxation magnetization returns are the same in both images ...
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No products found
because this supplier's products are not listed.
Martina Damo, et al.,
bioRxiv - Immunology 2020
Quote:
293T cells were cultured in complete DMEM (10% HI-FBS, 1x Pen/Strep) and then transfected using the LipoD293 Transfection reagent (SignaGen Laboratories, cat. SL100668) according to manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Haiyan Jia, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... Small RNA-seq library construction was performed using the Perkin Elmer’s Bio Scientific NEXTflex Small RNA-Seq Kit v3 and bar-coded with individual tags following the manufacturer’s instructions (Bioo Scientific Corp, Austin, TX). Libraries were prepared on a Perkin Elmer’s Sciclone G3 NGS Workstation Liquid Handling System ...
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No products found
because this supplier's products are not listed.
Sung-Eun Choi, et al.,
bioRxiv - Pathology 2020
Quote:
... Total protein in the hydrolysed sample was also measured using the Total Protein Assay Kit (QuickZyme) and the relative amount of collagen per protein was analysed.
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No products found
because this supplier's products are not listed.
Ayan Rajgarhia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and protein extraction was carried out using a total protein extraction Kit (BioChain Institute, Inc. Hayward, CA). Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Yingyan Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
C1-INH protein levels were measured using the SPAplus automated protein analyzer (The Binding Site Group Ltd, Birmingham, UK) – which utilizes turbidimetric measurement to determine protein level – according to manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Paresh P Kulkarni, et al.,
bioRxiv - Cell Biology 2023
Quote:
Protein C activity in WT and Apoh-/- plasma was performed using the Chromogenix Coamatic Protein C activity kit (Diapharma). Briefly ...
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
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No products found
because this supplier's products are not listed.
J. Ignacio Gutiérrez, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 100 uM Gal4–VP16 (Protein One, P1019-02) and 100 uM ATP or AMP-PNP ...
-
No products found
because this supplier's products are not listed.
Liina Hannula, et al.,
bioRxiv - Microbiology 2022
Quote:
... RBD wt (aa 319-541 of the S protein) and S1 wt (aa 14-681 of the S protein) from Medix Biochemica; RBD single mutants K417N ...
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No products found
because this supplier's products are not listed.
Jyoti Kundu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Pre-stained protein ladder was procured from Real Biotech Corporation ...
-
No products found
because this supplier's products are not listed.
Claire Marquilly, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Purified protein was injected into guinea pigs by Squarix GmbH (Marl ...
-
No products found
because this supplier's products are not listed.
Silvia De Cicco, et al.,
bioRxiv - Neuroscience 2020
Quote:
... plus bone morphogenetic protein 4 (BMP4, 50 ng/mL, Immunotools), vascular endothelial growth factor (VEGF ...
-
No products found
because this supplier's products are not listed.
Ugo Sardo, et al.,
bioRxiv - Physiology 2023
Quote:
Liver proteins were extracted by physical dissociation (Ultra-turrax, IKA) in PEB Buffer (150 mM NaCl ...
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No products found
because this supplier's products are not listed.
Xavier Martiáñez-Vendrell, et al.,
bioRxiv - Microbiology 2019
Quote:
... vivax pLDH proteins expressed in insect cells (3001, ReliaTech GmbH, Germany) were used as reference material ...
-
No products found
because this supplier's products are not listed.
Daniel Neumeier, et al.,
bioRxiv - Immunology 2021
Quote:
... protein extracts from commercial bone marrow tissue (Zyagen, MT-704-C57) as well as self-made extracts from spleen and bone marrow were tested at a 100ug/ml coating concentration ...
-
No products found
because this supplier's products are not listed.
Megha Subramanian, et al.,
bioRxiv - Genomics 2022
Quote:
... TTR protein levels were measured with mouse prealbumin kit from ALPCO, 41-PALMS-E01 (serum diluted 1:4000) ...
-
No products found
because this supplier's products are not listed.
Dennis S. Metselaar, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... glial fibrillary acidic protein (GFAP) (1:500; BT46-5002–04, BioTrend), S100 (1:1000 ...
-
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Leander Rohr, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Proteins were transiently expressed using the AGL1 Agrobacterium tumefaciens strain (Lifeasible), as previously described (Hecker et al. ...
-
No products found
because this supplier's products are not listed.
Clay D. Jackson-Litteken, et al.,
bioRxiv - Microbiology 2022
Quote:
... Purified protein was then used by Antibody Research Corporation (St. Peters, MO) to generate polyclonal rabbit antibody.
-
No products found
because this supplier's products are not listed.
Sunada Khadka, et al.,
bioRxiv - Biochemistry 2022
Quote:
... hygromycin resistance marker and red fluorescent protein (RFP) were purchased from Cellecta. For transfection ...
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Inc., Cat# CKH113).
-
No products found
because this supplier's products are not listed.
Chance J. Cosgriff, et al.,
bioRxiv - Microbiology 2019
Quote:
... Precipitated proteins were washed with 1 mL of 100% ethanol (Decon Laboratories Inc.) and incubated at 4°C for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Mayu Okada, et al.,
bioRxiv - Biophysics 2021
Quote:
... alignment of the protein was achieved using 20.0 mg/ml Pf1 phage (ASLA Biotech) in the NMR buffer ...
-
No products found
because this supplier's products are not listed.
Robert S. Kellar, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The proteins were dissolved into 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Oakwood Chemical, Estill, SC). The biomimetic WHDs were prepared using a ratio of 9:1 collagen to rhTE ...
-
No products found
because this supplier's products are not listed.
Elena A. Andreeva, et al.,
bioRxiv - Biophysics 2022
Quote:
... the protein solution (∼ 5 ml) was circulated with a peristaltic pump (Gilson Minipuls 3) through a 2 mm X-ray quartz capillary in a closed loop ...
-
No products found
because this supplier's products are not listed.
Tsuyoshi Hattori, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The neurons were cultured with or without SPARCL1 recombinant protein (80 nM, ATGP3891, NKMAX) from day 8 to day 14 as previously done (34) ...
-
No products found
because this supplier's products are not listed.
Xiaofeng Zheng, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Insulin and protein content of the lysate were measured using Mouse Insulin ELISA (Mercodia) and BCA assay (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Jeremy Ariey-Bonnet, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... The reagents (compound, protein, and fluorophore) were dispensed using an Echo550 acoustic dispenser (Labcyte): 100 nL of compound (from a 100% DMSO stock at 10 mM ...
-
No products found
because this supplier's products are not listed.
Zhouyi Rong, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Mouse Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit (RD-IP10-Mu, Reddot biotech) were used in this study.
-
No products found
because this supplier's products are not listed.
Stijn De Munter, et al.,
bioRxiv - Immunology 2023
Quote:
... 10 μM Rho-associated coiled–coil containing protein kinase (ROCK) inhibitor Y-27632 (Abmole) and 5 μM A83-01 (Tocris Bioscience)) ...
-
No products found
because this supplier's products are not listed.
Sarah G. Seman, et al.,
bioRxiv - Immunology 2024
Quote:
... These beads were either coated with purified protein derivative (PPD, AJ Vaccines, Copenhagen, Denmark) or left uncoated ...
-
No products found
because this supplier's products are not listed.
David S. Yang, et al.,
bioRxiv - Biophysics 2020
Quote:
... Protein and incubation buffer were filtered with 0.22 μm Polyethersulfone (PES) (CELLTREAT Scientific Products, 229746) syringe filters ...
-
No products found
because this supplier's products are not listed.
Mengqi Luo, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Membranes with protein samples subsequently reacted with anti-ACTB (1:5000, pAb, ZEN BIO, China), anti-SCRN2 (1:500 ...
-
No products found
because this supplier's products are not listed.
Elodie Billey, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Antibodies against the LARP6C protein were custom made (at Agro-Bio, La Ferté St Aubin, France) through immunization of rabbits with peptide “KRTSQFTDRDREELGQ” (amino acids 220-235) ...
-
No products found
because this supplier's products are not listed.
Xi Chen, et al.,
bioRxiv - Bioengineering 2021
Quote:
... hFIX protein was detected by a goat-anti-hFIX antibody (1:2000; Affinity Biologicals, GAFIX-AP). Mouse-anti-β-actin antibody (1:5000 ...
-
No products found
because this supplier's products are not listed.
Shima Shahbaz, et al.,
bioRxiv - Immunology 2020
Quote:
... The SARS-CoV-19 spike receptor binding domain protein was purchased from VIROGEN (Cat#00224-V), conjugated with dye using Fluorescent protein labeling kit according to the manufacturing protocol (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Carmanah Hunter, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Polysialylated proteins were eluted by incubating twice with 200 μL 25 mg/mL colominic acid (Carbosynth) at room temperature for 1 – 2 h and collecting the supernatant ...
-
No products found
because this supplier's products are not listed.
Machmouchi Dana, et al.,
bioRxiv - Microbiology 2024
Quote:
... ZIKV infectivity was assessed using the mouse anti-E protein mAb 4G2 (RD-Biotech, Besançon, France). Antibody donkey anti-mouse Alexa Fluor 488 IgG (Invitrogen ...