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No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
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The recombinant human MSLN is expressed as a 317-amino acid protein consisting of Glu296 -...
Cat# MSLN-001H,
100ug , USD $699
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Jonathan H. Shrimp, et al.,
bioRxiv - Biochemistry 2020
Quote:
Recombinant Human TMPRSS2 protein expressed from Yeast (human TMPRSS2 residues 106-492, N-terminal 6x His-tag) (Cat # TMPRSS2-1856H) was acquired from Creative BioMart (Shirley, NY). Peptides obtained from Bachem include ...
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No products found
because this supplier's products are not listed.
Sharon R. Stevens, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR(Ho et al. ...
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No products found
because this supplier's products are not listed.
Angela M. Getz, et al.,
bioRxiv - Neuroscience 2021
Quote:
... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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No products found
because this supplier's products are not listed.
Hans-Georg Sprenger, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... MITO-Tag mice (The Jackson Laboratory #032290) were described earlier 16 and to generate whole body-expressing MITO-Tag mice ...
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No products found
because this supplier's products are not listed.
Niklas Byczkowicz, et al.,
bioRxiv - Neuroscience 2019
Quote:
... the non-recorded ON was transferred in a different incubation chamber (Leica HI 1210) that provided similar incubation conditions to the recorded nerve while preventing exposure to ZD7288 and 8-Br-cAMP ...
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No products found
because this supplier's products are not listed.
Hui Chen, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
-
No products found
because this supplier's products are not listed.
Jürgen G. Okun, et al.,
bioRxiv - Physiology 2020
Quote:
... or protein-enriched (80%E protein, 10% carbohydrate, 10% fat; D16080303, Research Diets, USA) diets for a period of 3 weeks with various metabolic phenotyping tests ...
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No products found
because this supplier's products are not listed.
James N. Sleigh, et al.,
bioRxiv - Neuroscience 2023
Quote:
... cells were resuspended in 320 μl of fresh DRG media (F12, 10% hi-FBS, 1% Pen/Strep, 20 ng/ml mouse GDNF (Peprotech #450-44), 50 ng/ml mouse NGF (Peprotech #450-34) ...
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No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
-
No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
-
No products found
because this supplier's products are not listed.
Feng Long, et al.,
bioRxiv - Genomics 2020
Quote:
... SMOC2 EC domain and Mut SMOC2 EC domain containing the His tag were synthesized by Bioss Biotechnology ...
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No products found
because this supplier's products are not listed.
Ashok Pabbathi, et al.,
bioRxiv - Biophysics 2021
Quote:
... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
Cristina M. Ostalé, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and after standard treatments the Salm protein was eluted from Histidine columns and visualized by western blot using mouse anti-His (1:1000; Dianova) and anti-Salm (1:200 ...
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No products found
because this supplier's products are not listed.
David M. Zuckerman, et al.,
bioRxiv - Microbiology 2022
Quote:
... The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD, Cocalico, Reamstown, PA; His-PilQOlut, Eurogentec, Seraing, BE). Sera were tested for cross-reactivity by immunoblotting lysates from wildtype M ...
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No products found
because this supplier's products are not listed.
Qiannan Zhang, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and MBP (PurKine MBP-Tag Dextrin Resin 6FF, Abbkine) in turn ...
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No products found
because this supplier's products are not listed.
Andrea Toledo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and the V5 tag recombinant Fab fragment (Abnova, RAB00032) were coupled NHS-derived dyes using the same protocol as above but without the size-exclusion chromatography purification step.
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No products found
because this supplier's products are not listed.
Kui K. Chan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Kari H. Ecklund, et al.,
bioRxiv - Cell Biology 2021
Quote:
Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
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No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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No products found
because this supplier's products are not listed.
Lee Admoni-Elisha, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... His-SLUG or BSA diluted in PBS were added to a 96-well plate (Greiner Microlon) and incubated for 1 hour at room temperature followed by blocking with 3% BSA for 30 min ...
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No products found
because this supplier's products are not listed.
Maxime Batsch, et al.,
bioRxiv - Microbiology 2024
Quote:
... a 20x objective (Leica, HI PLAN I 20x/0,30 PH1, with P. putida-P. veronii, or a Nikon CFI S Plan Fluor ELWD 20XC MRH08230 ...
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No products found
because this supplier's products are not listed.
Felix O’Farrell, et al.,
bioRxiv - Genetics 2023
Quote:
... TAGs were assessed through colorimetric assays using 96-well microtiter plates and an Infinite M200Pro multifunction reader (TECAN). The assays were performed as previously described 18 ...
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No products found
because this supplier's products are not listed.
Meagan N. Esbin, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The media was then replaced with 100µLper well supplemented F12-K medium with 500nM JF646 Halo-tag ligand and 1:1000 MembraneSteady-488nm (Biotium).
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No products found
because this supplier's products are not listed.
Terry R. Suk, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and 36 µg rabbit anti-HA-tag (Cell Signalling Technology #3724) followed by fluorescently conjugated secondary antibodies in a 3:2 primary:secondary molar ratio (Jackson ImmunoResearch). Samples were incubated in EasyIndex (LifeCanvas Technologies ...
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No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
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No products found
because this supplier's products are not listed.
Gaurav Kumar, Sharmistha Sinha,
bioRxiv - Biophysics 2022
Quote:
Protein-protein interaction studies are carried out using Fortebio OctateK2 (Molecular Devices, USA). 0.5 mg/ml of PduBB’ or PduCDE (PduCDE in case of enzyme-shell interaction study ...
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No products found
because this supplier's products are not listed.
He Liu, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
The cytoplasmic and nucleic protein were extracted separately using the protein extraction kit (Boster) according to the manufacture’s introduction ...
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No products found
because this supplier's products are not listed.
Lindsay G. Serene, et al.,
bioRxiv - Microbiology 2023
Quote:
L929 fibroblasts were rapidly thawed in a 37°C water bath before being washed once in DMEM and 10% HI-FBS and transferred to a T-25 tissue culture treated flask (Sarstedt, Nümbrecht, Germany) in 10 mL DMEM and 10% HI-FBS with 100 U/mL penicillin/streptomycin ...
-
No products found
because this supplier's products are not listed.
Jingqi Zhou, et al.,
bioRxiv - Genomics 2021
Quote:
Zebrafish’s bone morphogenetic protein (BMP) and bone morphogenetic protein receptor genes were used as query to search the BMP and BMP receptor gene families in P ...
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No products found
because this supplier's products are not listed.
Balakrishnan Solaimuthu, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
-
No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
-
No products found
because this supplier's products are not listed.
Krishnakanth Kondabolu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... guinea pig anti-neuronal nuclei protein (NeuN, also known as hexaribonucleotide-binding protein 3; 1:500, 266004, Synaptic Systems, RRID:AB_2619988); goat anti-nitric oxide synthase (NOS ...
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With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
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Ethan L. Morgan, et al.,
bioRxiv - Microbiology 2020
Quote:
... Proteins were detected using WesternBright ECL (Advansta, USA) and visualised on X-ray film.
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No products found
because this supplier's products are not listed.
Changrui Lu, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Boyang Zhao, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
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No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
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No products found
because this supplier's products are not listed.
Ana Zúñiga, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... into 500μL of Azure Hi-Def medium (Teknova, 3H5000) supplemented with 0.4% of glycerol ...
-
No products found
because this supplier's products are not listed.
Haruna Fujioka, Yasukazu Okada, Masato S. Abe,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... we attached 2D barcode tags (2.0 mm × 2.0 mm) to the individuals with instant glue (3M Scotch ...
-
No products found
because this supplier's products are not listed.
Satoshi Watanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
The PiggyBac-cumate-ERGIC-53-PA tag plasmid along with the Super PiggyBac Transposase Expression Vector (System Bioscience) was transfected to generate a stable HEK293T cell line with cumate inducible ERGIC-53 expression ...
-
No products found
because this supplier's products are not listed.
Jonathan M Downie, et al.,
bioRxiv - Genetics 2019
Quote:
... HEK293T cells were incubated in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (HI FBS, Genesee Scientific), and 1% pen/strep (P/S ...
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No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
-
No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... VHHs were enzymatically biotinylated in their BAP tag by incubating 1 mg of purified VHHs with 10 µM of ATP (Alfa Aesar, Haverhill, MA, Cat#CAAAJ61125-09), 100 µM of D-(+)-biotin (VWR ...