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No products found
because this supplier's products are not listed.
Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Shan Jiang, et al.,
bioRxiv - Microbiology 2023
Quote:
... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
-
No products found
because this supplier's products are not listed.
Abby V. McGee, et al.,
bioRxiv - Genomics 2023
Quote:
B2M: APC anti-human B2M antibody (Biolegend, 316312)
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No products found
because this supplier's products are not listed.
Mélanie Bodnar-Wachtel, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
-
No products found
because this supplier's products are not listed.
Carina Shiau, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... B2M (Abcam, EP2978Y), PanCK (Novus ...
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No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Recombinant cynomolgus IL 11 (cIL11, 90925-CNCE, Sino Biological), human IL11 (hIL11 ...
-
No products found
because this supplier's products are not listed.
Kun Meng, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and 6×His-Flag-Ub was conducted using Ni-NTA agarose (Qiagen), following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Thomas Mandel Clausen, et al.,
bioRxiv - Microbiology 2020
Quote:
... and SARS-CoV-2 spike protein (ECD, His & Flag Tag) (GenScript Z03481). Proteins were biotinylated using EZ-Link™ Sulfo-NHS-Biotin ...
-
No products found
because this supplier's products are not listed.
Devin Dersh, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-B2M (Dako); anti-TAP1 (EMD Millipore) ...
-
No products found
because this supplier's products are not listed.
Maya Shofa, et al.,
bioRxiv - Microbiology 2023
Quote:
... and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB ...
-
No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and cynomolgus IL11 were measured on Biacore T200 (GE Healthcare). VVB011 was immobilised on an anti-mouse capture chips and a concentration range of each of the analytes (hIL11 ...
-
No products found
because this supplier's products are not listed.
Daniele Tedesco, et al.,
bioRxiv - Biophysics 2021
Quote:
... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
-
No products found
because this supplier's products are not listed.
Parishmita Sarma, et al.,
bioRxiv - Biochemistry 2022
Quote:
... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
-
No products found
because this supplier's products are not listed.
Sieglinde De Cae, et al.,
bioRxiv - Microbiology 2023
Quote:
... His-tagged recombinant human FcRn (FCGRT-B2M heterodimer) protein was purchased from ACROBiosystems. Bebtelovimab biosimilar and a human IgG1 isotype control antibody were used as controls in the assay ...
-
No products found
because this supplier's products are not listed.
Katherine Warre-Cornish, et al.,
bioRxiv - Neuroscience 2020
Quote:
B2M was knocked down using lentiviral particles containing B2M-targeting shRNA (OriGene, TL314543V, Virus A). Non-targeting scramble shRNA from the same kit was used as control ...
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No products found
because this supplier's products are not listed.
Catherine M. Mageeney, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibodies (Anti-FLAG: Sigma, F3165; Anti-His: Cell Signaling Technologies, Danver, MA, 2366S) were used at 1:1000 ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Ravi Jagasia, et al.,
bioRxiv - Neuroscience 2022
Quote:
NPCs derived from cynomolgus macaque (Cips_BC671A_NSC line generated at Roche) were differentiated in NPC differentiation medium (42) ...
-
No products found
because this supplier's products are not listed.
Jisun So, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... The tissues were incubated with (1) anti-B2M (Proteintech) or (2 ...
-
No products found
because this supplier's products are not listed.
Christian L. Egly, et al.,
bioRxiv - Biochemistry 2024
Quote:
... All HEK293 cells were cultured in HEK293 media consisting of Minimum Essential Media (MEM, Corning) containing 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Amy Q. Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and male Cynomolgus monkey plasma (BioIVT) using a Pierce Rapid Equilibration Dialysis (RED ...
-
No products found
because this supplier's products are not listed.
Yuichi Tsuchiya, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
-
No products found
because this supplier's products are not listed.
Ignasi Casanellas, et al.,
bioRxiv - Bioengineering 2021
Quote:
... as well as B2M (Bio-Rad, qHsaCID0015347) and RPL24 (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Adrian Westhaus, et al.,
bioRxiv - Bioengineering 2022
Quote:
Cynomolgus and Rhesus macaque primary hepatocytes were purchased from Lonza (Cat# MKC118 and Cat# MKR103). Human hepatocytes were also purchased from Lonza (Cat# HUM181971 ...
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No products found
because this supplier's products are not listed.
Nithya Sivaram, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... αKO cells with reduced levels of B2m (referred to as αKO/shB2m) were generated by infecting αKO cells with B2m shRNA lentiviral particles (Santa Cruz Biotechnology, sc-29593-V). 48 hours after infection ...
-
No products found
because this supplier's products are not listed.
Noelle Anderson, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... and sequencing (Illumina Hi-Seq) were performed by Iridian Genomes ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Amit Laxmikant Deshmukh, et al.,
bioRxiv - Genetics 2021
Quote:
... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
-
No products found
because this supplier's products are not listed.
Lu Cui, et al.,
bioRxiv - Immunology 2020
Quote:
... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
-
No products found
because this supplier's products are not listed.
Alexandre B. Leitão, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... Sensifast Hi-Rox SyBr kit (Bioline) was used to perform the RT-qPCR on a StepOnePlus system (ThermoFisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
-
No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
No products found
because this supplier's products are not listed.
Krishnendu Chakraborty, et al.,
bioRxiv - Immunology 2021
Quote:
... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
-
No products found
because this supplier's products are not listed.
Allen G. Sanderlin, et al.,
bioRxiv - Microbiology 2022
Quote:
... and precipitated culture supernatants (equivalent to 2 mL of culture supernatant prior to precipitation) were analyzed by western blot using rabbit anti-FLAG and HRP-conjugated mouse anti-His (ABclonal #AE028).
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Flag-PFDN2 and Flag-VIM) and the bacterial expression vectors pReceiver-B11 (His-NSMCE2 and His-PFDN2) were purchased from Genecopoeia, Inc ...
-
Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1...
Cat# B26102, SKU# B26102-5 mL,
5 mL, $999.00
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Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... Immunoprecipitation was performed with anti-His or Flag magnetic beads (Bimake, Texas, US), according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Elizabeth D. Luczak, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... FLAG (Rockland), GAPDH (Cell Signaling) ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Tim Nicholson-Shaw, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... FLAG peptide (ApexBio, A6002) was added to lysates to a concentration of 1 µg/ml to reduce non-specific interactions ...
-
No products found
because this supplier's products are not listed.
Tomotaka Okamura, et al.,
bioRxiv - Immunology 2020
Quote:
One hundred microliters of whole blood from each of the cynomolgus macaques was stained with combinations of fluorescence-conjugated monoclonal antibodies: anti-CD3 (clone SP34-2, Alexa700; BD), anti-CD4 (clone L200 ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Masaya Yamaguchi, et al.,
bioRxiv - Microbiology 2019
Quote:
Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial ...
-
No products found
because this supplier's products are not listed.
Chrissie E. B. Ong, et al.,
bioRxiv - Immunology 2020
Quote:
... B2M negative cells were selected and bulk-sorted using cell sorter Moflo Astrios EQ (Beckman Coulter).
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
because this supplier's products are not listed.
Shreya Ahuja, Iulia M. Lazar,
bioRxiv - Immunology 2021
Quote:
... TGFβ1 (HEK293 derived) and TGFβ2 (HEK293 derived) were acquired from Peprotech (Rocky Hill, NJ). Primary antibody [mouse monoclonal IgG anti-Vimentin (V9)] ...