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No products found
because this supplier's products are not listed.
Jinqiang Liu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Anti-Flag M2 affinity gel (Sigma), anti-Myc (9E10 from Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Gianmatteo Vit, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Anti-FLAG M2 Affinity gel (Sigma-Merck) was equilibrated in lysis buffer and gently shaked at 4°C for 3 hours with the lysate ...
-
No products found
because this supplier's products are not listed.
Danielle L. Tomasello, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using an anti-FLAG affinity resin (Genscript). Lysates were incubated with 40 µl of beads overnight at 4°C with rotation ...
-
No products found
because this supplier's products are not listed.
Sha Sun, Xia Li, Malaiyalam Mariappan,
bioRxiv - Cell Biology 2022
Quote:
... Rat anti-FLAG L5 affinity gel (Biolegend, #651503), Protein A agarose (Repligen ...
-
No products found
because this supplier's products are not listed.
Ambrocio Sanchez, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 100 μl of M2 anti-Flag affinity gel (Thermo Fisher Scientific) was added to the soluble extract for 2 hours 30 min at 4°C ...
-
No products found
because this supplier's products are not listed.
Shu Yang, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... followed by incubation with anti-FLAG or anti-HA antibody coupled beads (Anti-M2 Affinity Matrix from Sigma; Anti-HA Affinity Matrix from Roche) overnight at 4 °C ...
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Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently...
Cat# B23101, SKU# B23101-1 ml Kit,
1 ml Kit, $319.00
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Chieh-Hsiang Tan, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... anti-Flag Affinity Gel (Bimake, B23101), or anti-ITFG1 antibody-conjugated agarose ...
-
No products found
because this supplier's products are not listed.
Dipan Roy, et al.,
bioRxiv - Plant Biology 2024
Quote:
... The immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with anti-HA antibodies (Anti-HA High Affinity Roche 3F/10, Sigma-Aldrich, and anti-HA Abcam). At least three independent biological replicates were performed per experiment ...
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No products found
because this supplier's products are not listed.
Caillan Crowe-McAuliffe, et al.,
bioRxiv - Microbiology 2020
Quote:
100 µL of well mixed anti-FLAG M2 Affinity Gel aliquots were loaded on columns (Micro Bio-Spin Columns, Bio-Rad) and washed two times with 1 mL of cell lysis buffer by gravity flow ...
-
No products found
because this supplier's products are not listed.
Georgios Batsios, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... FLAG (Cell Signaling, anti-FLAG M2, # 14793). β-Actin (Cell Signaling ...
-
No products found
because this supplier's products are not listed.
Wei Peng, et al.,
bioRxiv - Biophysics 2020
Quote:
... The supernatant was loaded into gravity column containing anti-FLAG M2 affinity gel (GE Healthcare). The resin was washed with wash buffer (25 mM HEPES pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Shu Zhang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Pre-cleared samples were incubated with ANTI-FLAG® M2 Agarose Affinity Gel or Anti-V5 Agarose Affinity Gel (Bethyl Group) for 4 hours at 4 °C ...
-
No products found
because this supplier's products are not listed.
Zhihao Wu, et al.,
bioRxiv - Cell Biology 2019
Quote:
... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 5U Enterokinase (cat#: P8070S, NEB) in the 1x EK reaction buffer (20 mM Tris-HCl ...
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No products found
because this supplier's products are not listed.
Wenying Yu, et al.,
bioRxiv - Microbiology 2020
Quote:
... anti-Flag (Clontech, USA) and anti-GFP (Sigma ...
-
No products found
because this supplier's products are not listed.
Yi Liu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... were then incubated with EZview™ Red Anti-HA Affinity Gel (30 μL; MilliporeSigma, MA) or ChromoTek Myc-Trap® Agarose (30 μL; Proteintech, IL) at 4°C overnight ...
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No products found
because this supplier's products are not listed.
Tengyu Xie, et al.,
bioRxiv - Biophysics 2021
Quote:
... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
-
No products found
because this supplier's products are not listed.
Mark A Gutierrez, Brett E Dwyer, Santos J Franco,
bioRxiv - Neuroscience 2020
Quote:
... Samples were incubated for 3 hours at 4°C with Anti-DYDDDDK Affinity Gel (Rockland, RRID:AB_10704031). After incubation ...
-
No products found
because this supplier's products are not listed.
Hongchang Zhao, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-FLAG (Abclonal, AE005), Goat anti-mouse AlexaFluor 680 secondary antibody (A21057 ...
-
No products found
because this supplier's products are not listed.
Joshua J. Black, Arlen W. Johnson,
bioRxiv - Molecular Biology 2021
Quote:
... rat anti-FLAG (Agilent), mouse anti-GFP (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Gahyang Cho, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... mouse anti-FLAG (1:500, Santa Cruz), rabbit anti-AMDA (1:500 ...
-
No products found
because this supplier's products are not listed.
Hyun-Soo Kim, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... was incubated with Anti-Flag M2 Affinity Gel or Ubiquitination Affinity Beads (UAB, Cytoskeleton UBA01-Beads) overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Lei Yang, et al.,
bioRxiv - Physiology 2022
Quote:
... affinity purified anti-rabbit antibody (Jackson Immunoresearch). For selection of HCN3+ cells ...
-
No products found
because this supplier's products are not listed.
Yang Su, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-ALPPL2 affinity-purified rabbit polyclonal antibody (Origene) or anti-ALPPL2 mouse antibody (Clone SPM593 ...
-
No products found
because this supplier's products are not listed.
Ik-Jung Kim, et al.,
bioRxiv - Microbiology 2022
Quote:
... Flag-Spike-Flag (Addgene #: 156418), and David Nemanzee [32] (SARS-CoV Spike ΔC28 (Addgene # ...
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No products found
because this supplier's products are not listed.
Inês M.A. Ribeiro, et al.,
bioRxiv - Genetics 2022
Quote:
... anti-FLAG rat antibody dilution 1:300 (Novus Biologicals, catalogue # NBP1-06712 ...
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No products found
because this supplier's products are not listed.
Izabela Wojciechowska, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Affinity Analysis software (NanoTemper) was used to analyze binding affinities from changes in fluorescence ...
-
No products found
because this supplier's products are not listed.
Zhihao Wu, et al.,
bioRxiv - Cell Biology 2019
Quote:
... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
-
No products found
because this supplier's products are not listed.
Pooja Pandey, et al.,
bioRxiv - Plant Biology 2020
Quote:
... anti-HA and anti-FLAG antibodies produced in rat (Chromotek, UK) were used as primary antibodies ...
-
No products found
because this supplier's products are not listed.
Madison A. DeWinter, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... Anti-FLAG donor beads (Perkin Elmer) were used to immobilize the peptide hormone and either Protein A (Fc tagged GLP-1 receptor ...
-
No products found
because this supplier's products are not listed.
Krishna Vasant Mutanwad, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 50 μL of anti-FLAG beads (Miltenyi Biotec) were added to the remaining plant extract and the samples were incubated on a rotor at 4°C for 30 minutes ...
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No products found
because this supplier's products are not listed.
Skye A. Edwards, et al.,
bioRxiv - Bioengineering 2023
Quote:
... specifically donkey anti-goat IgG NL493 affinity purified PAb (R&D Systems Inc), was introduced following washing with PBS ...
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No products found
because this supplier's products are not listed.
Lucas M. Demey, Ritam Sinha, Victor J. DiRita,
bioRxiv - Microbiology 2022
Quote:
... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
-
No products found
because this supplier's products are not listed.
Roman Generalov, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... High-affinity FcγRI (Sino Biological) and AP-streptavidin were added separately ...
-
No products found
because this supplier's products are not listed.
Xiao-Ru Chen, et al.,
bioRxiv - Biophysics 2022
Quote:
... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
-
No products found
because this supplier's products are not listed.
Sarah Krieg, et al.,
bioRxiv - Microbiology 2020
Quote:
... TALON metal affinity resin (BD Bioscience), GFP-Trap magnetic agarose beads (Chromotek ...
-
No products found
because this supplier's products are not listed.
Coralie E. Salesse-Smith, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Anti-FLAG (Agrisera) and anti-Actin (Agrisera ...
-
No products found
because this supplier's products are not listed.
Valerio Marino, et al.,
bioRxiv - Biochemistry 2023
Quote:
... rabbit anti-DDDDK (FLAG) tag (Genetex), rabbit anti-histone H3 (R&D) ...
-
No products found
because this supplier's products are not listed.
Jie Shi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... acceptor: mAb anti FLAG M2-d2 (61FG2DLB, Cisbio). Further HTRF measurement against ‘No Target’ allowed for discrimination of myosin VI isoform 1-specific hits ...
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No products found
because this supplier's products are not listed.
Simon Bressendorff, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The anti-AGO1-N-coil antibody was affinity purified by Eurogentec from sera collected from a rabbit immunized with a 16-amino acid peptide from the N-coil of AGO1 (F177-C192 ...
-
No products found
because this supplier's products are not listed.
Lesia Rodriguez, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
-
No products found
because this supplier's products are not listed.
Àngels Mateu-Regué, et al.,
bioRxiv - Cell Biology 2020
Quote:
Total lysate and FLAG immunoprecipitation samples were loaded into a 10% RunBlueTM SDS protein gel (Expedeon) and separated by electrophoresis ...
-
No products found
because this supplier's products are not listed.
Nobuyuki Oguri, et al.,
bioRxiv - Pathology 2023
Quote:
... anti-platelet glycoprotein (GP) IIb/IIIa (Sheep monoclonal, Affinity Biologicals Inc., Ancaster, Canada), glycophorin A (Dako/Agilent) ...
-
No products found
because this supplier's products are not listed.
Otoya Kitaoka, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and anti-vesicular glutamate transporter 1 (anti-vGLUT1) at 1:2,000 (rabbit polyclonal, affinity-purified, Synaptic Systems, Göttingen, Germany). Secondary antibodies were applied using Alexa Fluor 488 or 594 (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Scott P. Souza, et al.,
bioRxiv - Immunology 2021
Quote:
High-affinity protein binding microplates (Corning) were coated overnight goat anti-mouse IgM (1mg/ml ...
-
No products found
because this supplier's products are not listed.
Tim Nicholson-Shaw, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... FLAG peptide (ApexBio, A6002) was added to lysates to a concentration of 1 µg/ml to reduce non-specific interactions ...
-
No products found
because this supplier's products are not listed.
Yimiao Qu, et al.,
bioRxiv - Systems Biology 2019
Quote:
... The PVDF membrane with the anti-FLAG and anti-α-tubulin antibody were then incubated with 1:10000 anti-mouse IgG (C50721-02; LI-COR) and anti-rabbit IgG (C50618-03 ...
-
No products found
because this supplier's products are not listed.
Thomas S. McAlear, Susanne Bechstedt,
bioRxiv - Biophysics 2021
Quote:
... followed by Streptactin affinity chromatography (IBA Lifesciences, Germany). Purified CKAP2 was eluted with BRB80 (80 mM PIPES-KOH pH 6.85 ...
-
No products found
because this supplier's products are not listed.
Laura Miguel-Romero, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Gels were stained with Gel Red (Biotium) for 10min in shaking conditions and analysed by ChemiDoc (BioRad).
-
No products found
because this supplier's products are not listed.
Farhana Rahman, et al.,
bioRxiv - Cell Biology 2020
Quote:
... anti-FLAG (Genecopoeia) and anti-KDEL (Enzo Life Sciences ...
-
No products found
because this supplier's products are not listed.
Yisong Qian, et al.,
bioRxiv - Immunology 2021
Quote:
Flag-NP and Flag-control vectors were transfected to HLMEC by electroporation using Nucleofector device (Lonza) and Nucleofector kits (Lonza ...