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No products found
because this supplier's products are not listed.
Ashok Pabbathi, et al.,
bioRxiv - Biophysics 2021
Quote:
... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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Recombinant Human CD28 (P10747-1) extracellular domain (Met 1-Pro 152), fused with a...
Cat# CD28-3910H,
50ug , USD $298
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Jonathan H. Shrimp, et al.,
bioRxiv - Biochemistry 2020
Quote:
Recombinant Human TMPRSS2 protein expressed from Yeast (human TMPRSS2 residues 106-492, N-terminal 6x His-tag) (Cat # TMPRSS2-1856H) was acquired from Creative BioMart (Shirley, NY). Peptides obtained from Bachem include ...
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No products found
because this supplier's products are not listed.
Jason Derks, Nikolai Slavov,
bioRxiv - Systems Biology 2023
Quote:
... and mTRAQ-Δ8 mass tags (SciEx, 4440015, 4427698 and 4427700), respectively ...
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No products found
Svenja Fritzlar, et al.,
bioRxiv - Microbiology 2023
Quote:
... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
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No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... virus containing supernatants were clarified using a 0.45 µM PDVF filter (Pall Corp, NY, USA # 4614) and pelleted by ultracentrifugation on a 15% sucrose cushion before resuspension in culture media to achieve a 20X concentration over the original volume ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
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No products found
because this supplier's products are not listed.
Liu He, et al.,
bioRxiv - Biochemistry 2022
Quote:
... coli strain BL21 (DE3) with an N-terminal (His)6-SUMO tag from a pE-SUMO-pro expression vector (LifeSensors). Cells were grown in 2YT media at 24°C to an OD600 of 0.8 and then induced with 0.1 mM isopropyl β-D-thiogalactopyranoside (IPTG ...
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No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Microbiology 2019
Quote:
... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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No products found
because this supplier's products are not listed.
Hoa Quynh Do, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and lipid-protein nanodiscs (MSP1D1-His-POPC, MSP1D1-His-DMPG, MSP1D1-His-DMPC, Cube Biotech) were used to solubilize in-situ synthesized PCFT ...
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No products found
because this supplier's products are not listed.
Kristin S. Fitzpatrick, et al.,
bioRxiv - Immunology 2022
Quote:
... and Vaccinia virus B8R (TSYKFESV, Biomatik).
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No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
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No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
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No products found
because this supplier's products are not listed.
Hans-Georg Sprenger, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... MITO-Tag mice (The Jackson Laboratory #032290) were described earlier 16 and to generate whole body-expressing MITO-Tag mice ...
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No products found
because this supplier's products are not listed.
Qiannan Zhang, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and MBP (PurKine MBP-Tag Dextrin Resin 6FF, Abbkine) in turn ...
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No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
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No products found
because this supplier's products are not listed.
Justin J. Botterill, et al.,
bioRxiv - Neuroscience 2021
Quote:
Virus was delivered using a 500nL Neuros Syringe (#65457-02, Hamilton Company) attached to the stereotaxic apparatus with a probe holder (#751873 ...
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No products found
because this supplier's products are not listed.
Alexander J. Finney, et al.,
bioRxiv - Microbiology 2019
Quote:
... Nitrocellulose membranes were challenged with an anti-His-HRP antibody (Alpha Diagnostics) and a GeneGnome instrument (SynGene ...
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No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
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No products found
because this supplier's products are not listed.
Timothy P. Sheahan, et al.,
bioRxiv - Microbiology 2020
Quote:
... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
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No products found
because this supplier's products are not listed.
Leena Hussein Bajrai, et al.,
bioRxiv - Microbiology 2021
Quote:
... the plates were analyzed by observing virus-induced CPE by light microscope (Nikon-ECLIPSE-Ti), and plaque formation was determined by crystal violet staining (C0775 ...
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No products found
because this supplier's products are not listed.
M. Bormann, et al.,
bioRxiv - Microbiology 2021
Quote:
... the infectious virus was recovered by vortexing the carriers placed in plastic containers (SARSTEDT, Nümbrecht, Germany) filled with 2 mL DMEM for 1 minute ...
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No products found
because this supplier's products are not listed.
Youssouf Sereme, et al.,
bioRxiv - Microbiology 2020
Quote:
... The virus-infected MRC5 cells were collected and centrifuged at 700 × g for 15 minutes (Eppendorf Centrifuge 5810R). Supernatant was filtered by serial filtration steps to eliminate cells and cellular debris ...
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No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
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No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
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No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
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No products found
because this supplier's products are not listed.
Sabal Chaulagain, et al.,
bioRxiv - Microbiology 2020
Quote:
Swine PBMCs were obtained from swine whole blood through density gradient centrifugation using Sepmate15 (Stem cell technologies) and lymphocyte separation media (Corning) ...
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No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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No products found
because this supplier's products are not listed.
Feng Long, et al.,
bioRxiv - Genomics 2020
Quote:
... SMOC2 EC domain and Mut SMOC2 EC domain containing the His tag were synthesized by Bioss Biotechnology ...
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No products found
because this supplier's products are not listed.
Heithem M. El-Hodiri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Pigs were fed a swine-specific feed (Harlan Teklad Miniswine Diet 8753; Envigo, Hackensack, NJ) twice daily and provided water ad libitum ...
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No products found
because this supplier's products are not listed.
Boyang Zhao, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and σNS-ΔN17 were subcloned into the bacterial expression vector pET28 with an N-terminal His tag and a TEV protease cleavage site (Epoch Life Science). Escherichia coli DE3 cells (Novagen ...
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No products found
because this supplier's products are not listed.
Mukesh Kumar, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and M3 is M1 food supplemented with equal amounts of three saturated TAGs (TAG 42:0; TAG 48:0; TAG 54:0; Larodan).
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No products found
because this supplier's products are not listed.
Roberto Espinoza-Corral, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 150 mM NaCl and 0.01% tween-20) overnight at 4°C (anti-PsbA; AS05 084A; anti-PsaB; AS10 695; anti-APC; AS08 277; Agrisera, anti-His tag; TA150087; OriGene). The membrane was washed 3 times in TBST-T at room temperature for 15 minutes each wash followed by incubation with secondary polyclonal anti-rabbit antisera HRP for one hour at room temperature in TBS-T (Jackson ImmunoResearch ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
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No products found
because this supplier's products are not listed.
Chris Y. Cheung, et al.,
bioRxiv - Cell Biology 2021
Quote:
... His-KPNA5 protein was obtained from LifeSpan BioSciences, Inc ...
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No products found
because this supplier's products are not listed.
Sandra D. Chanez-Paredes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... mouse anti-6-His (Bio X Cell, AB_2687790) was used for western blot (3 µg/ml) ...
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No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
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No products found
because this supplier's products are not listed.
Kerstin Voelkl, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti-His (Dianova, DIA-900-100, 1:1,000), chicken anti-EGFP (Invitrogen ...
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No products found
because this supplier's products are not listed.
Andrea Toledo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and the V5 tag recombinant Fab fragment (Abnova, RAB00032) were coupled NHS-derived dyes using the same protocol as above but without the size-exclusion chromatography purification step.
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No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
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No products found
because this supplier's products are not listed.
Stanley E Strawbridge, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Cells were plated on laminin coated Hi-Q4 dishes (Ibidi 80416) at single-cell density (500 cells/ 10 cm2 ...
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No products found
Yuanyuan He, et al.,
bioRxiv - Microbiology 2023
Quote:
Imaging plates for virus quantification were prepared by coating coverslip-bottom wells (Cellvis) with 0.18 mg/ml BSA-biotin in PBS overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Kari H. Ecklund, et al.,
bioRxiv - Cell Biology 2021
Quote:
Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
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No products found
because this supplier's products are not listed.
Advika Kamatar, et al.,
bioRxiv - Biophysics 2023
Quote:
... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Immunology 2023
Quote:
... Heat Inactivated fetal bovine serum (HI-FBS) was purchased from Atlanta Biologicals. IFN-α cytokine enzyme-linked immunosorbent assay (ELISA ...
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No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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No products found
because this supplier's products are not listed.
Liam Hudson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
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No products found
because this supplier's products are not listed.
Maria Kuzikov, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plates were transported to the CAPS-IT robotic system for the addition of virus dilution (MOI = 0.001) using a liquid handler (EVO 100, Tecan) to a final well volume of 60 μl ...
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No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...