-
No products found
because this supplier's products are not listed.
Giulia Manigrasso, et al.,
bioRxiv - Microbiology 2024
Quote:
The full-length human RANBP2 sequence was codon optimized for expression in Sf9 insect cells (Epoch Life Sciences). Residues 2148-2240 fused to a N-terminal GCN4 dimerization tag were subcloned into a pACEBac1 vector with a N-terminal 6xHis-ZZ tag followed by a TEV cleavage site ...
-
No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2020
Quote:
... the original stock of this virus (wild type, WT) was prepared by inoculation of Vero-TMPRSS2 cells with Mynox mycoplasma elimination reagent (Minerva Biolabs) [11] ...
-
No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
-
No products found
because this supplier's products are not listed.
Julie M. Button, Suchetana Mukhopadhyay,
bioRxiv - Microbiology 2021
Quote:
Five microliters of purified virus or pelleted cores were placed on a Formvar and carboncoated 300 mesh grid (Ted Pella Inc., Redding, CA) and stained with 1% uranyl acetate ...
-
No products found
because this supplier's products are not listed.
Magen E. Francis, et al.,
bioRxiv - Microbiology 2021
Quote:
... to confirm stability of the SARS-CoV-2 virus after culture in vDMEM (DMEM (Dulbecco’s Modified Eagle Medium) (Wisent Bioproducts (Cat # 319-005-CL)) ...
-
No products found
because this supplier's products are not listed.
Guodong Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Cells were harvested by cell lifter (Biologix, 70-2180) 72-h after co-transfection with PHP.eB (Addgene ...
-
No products found
because this supplier's products are not listed.
Hope M. Healey, Susan Bassham, William A. Cresko,
bioRxiv - Genetics 2021
Quote:
... we filtered cells through a 40uM cell strainer (Thomas scientific 1181X52) then washed the original tube twice with 100ul of 0.04% BSA in PBS and poured over the same cell strainer.
-
No products found
because this supplier's products are not listed.
Immacolata Porreca, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... T cells were lysed using DirectPCR (cell) (Viagen Biotech, LA, USA) lysis buffer supplemented with proteinase K (10 µg/mL ...
-
No products found
because this supplier's products are not listed.
Laura Maria Florez, et al.,
bioRxiv - Immunology 2023
Quote:
... composed of mouse endothelial cell medium with the addition of endothelial cell medium supplement kit (from Cell Biologics, Euromedex) containing fetal bovine serum ...
-
No products found
because this supplier's products are not listed.
Adham Safieddine, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cells were maintained in anti-bleaching live cell visualization medium (DMEMgfp; Evrogen), supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Ameya P. Jalihal, et al.,
bioRxiv - Cell Biology 2020
Quote:
... For live cell imaging cells were seeded on Delta T dishes (Bioptechs, 04200417C) and imaged on a Bioptechs temperature control module (Bioptechs ...
-
No products found
because this supplier's products are not listed.
Aline Pfefferle, et al.,
bioRxiv - Immunology 2019
Quote:
... The labeled NK cells were resuspended in Stem Cell Growth Medium (SCGM; CellGenix) supplemented with 10% human serum (Sigma) ...
-
No products found
because this supplier's products are not listed.
Anthony J. Domma, et al.,
bioRxiv - Microbiology 2023
Quote:
... cell culture media and cells were collected and the cells were homogenized using 10 strokes of a Dounce homogenizer (Wheaton Cat # 357542). The media/homogenate was cleared of cell debris by centrifugation at 2000 rpm for 10 min ...
-
No products found
because this supplier's products are not listed.
Kim S. Friedmann, et al.,
bioRxiv - Immunology 2020
Quote:
... 75 μg of total proteins were separated by 15 % SDS–polyacrylamide gel electrophoresis and afterwards transferred onto polyvinylidene difluoride membranes using a transblot transfer chamber (X-Cell SureLock™, Invitrogen Novex Mini-cell, or Mini-PROTEAN Tetra Cell, Biorad). Western blots were probed with anti-MART-1 antibodies (1:1000) ...
-
No products found
Doris Roth, et al.,
bioRxiv - Biophysics 2024
Quote:
... Cells were then seeded at a density of 30K cells/cm2 on PureCol (Advanced Biomatrix #5005) coated tissue culture dishes in airway epithelial growth media (Promocell #C-21160 ...
-
No products found
because this supplier's products are not listed.
Yurika Ito, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Single iPS cells carrying an inducible MYOD1 activation system were expanded in Primate ES cell medium (Reprocell) without bFGF and with 10µM of Y-27632 (Nacalai tesque ...
-
No products found
because this supplier's products are not listed.
Dinesh K Deochand, et al.,
bioRxiv - Immunology 2024
Quote:
... Cell-bead antibody complex was then washed with cell permeabilization buffer and incubated with pAG-MNase (EpiCypher), followed by addition of 2 mM CaCl2 and incubation for 2 h at 4°C ...
-
No products found
Fia B. Larsen, et al.,
bioRxiv - Biochemistry 2023
Quote:
Cells were treated with bortezomib (LC Laboratories) and chloroquine (Sigma ...
-
No products found
because this supplier's products are not listed.
Ibrahim Hawwari, et al.,
bioRxiv - Immunology 2023
Quote:
... cytokines were detected in cell-free supernatants or whole cell lysates by homogeneous time-resolved fluorescence technology (HTRF, Cisbio). A Human ProcartaPlexTM (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Rory Hennell James, et al.,
bioRxiv - Biochemistry 2022
Quote:
... protein complexes were overexpressed in BL21(DE3) cells and then extracted from cell membranes using lauryl maltose neopentyl glycol (LMNG; Anatrace). Protein complexes were then affinity-purified using StrepTactin XT resin (IBA ...
-
No products found
because this supplier's products are not listed.
Logan R. Hurst, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and cells were grown on YPD agar (RPI) plates containing 200 mg/L hygromycin B (Goldbio) ...
-
No products found
because this supplier's products are not listed.
Alexander Kirillov, et al.,
bioRxiv - Microbiology 2022
Quote:
... Cells were grown in standard LB medium (Amresco) with antibiotics and inducers (if necessary) ...
-
No products found
because this supplier's products are not listed.
Julian R. Smith, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were stimulated with recombinant IFNβ (PBL Assay Science) and IFNλ3 (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Constructs were amplified from insect cell codon-optimized DNA fragments (Bio Basic Inc., Canada) for protein production in High Five insect cells ...
-
No products found
because this supplier's products are not listed.
Megan Clapperton, et al.,
bioRxiv - Biophysics 2023
Quote:
... Two photomultiplier tubes (PMTs) (P30-01, Senstech, UK) were used for detection of reflection (PMT 1) and fluorescence (PMT 2).
-
No products found
because this supplier's products are not listed.
Camille Boudreau-Pinsonneault, et al.,
bioRxiv - Neuroscience 2021
Quote:
P30-40 Glast-CreER;R26R-EYFP mice received tamoxifen diluted in canola oil (Toronto Research Chemicals or Cedarlane Labs) by intraperitoneal injections for four consecutive days at 90µg/gram of body weight ...
-
No products found
because this supplier's products are not listed.
Xueyan Zhang, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 400 μg total RNA from virus-infected Vero-E6 cells was incubated with an anti-m6A antibody (Synaptic Systems, Goettingen, Germany) or an IgG antibody in 300 μL IP buffer (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
-
No products found
Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
-
No products found
because this supplier's products are not listed.
Norbert S. Hill, Matthew D. Welch,
bioRxiv - Microbiology 2021
Quote:
... CVCL_0549) were also obtained from the UCB-CCF and grown at 28°C in in Grace’s insect media (Gemini Bio-Products, 600-310) supplemented with 2% FBS (Gemini Bio-Products ...
-
No products found
because this supplier's products are not listed.
Dániel Molnár, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... The cells were disrupted using a cell disruptor (Scientific Industries SI-DD38 Digital Disruptor Genie Cell Disruptor ...
-
No products found
because this supplier's products are not listed.
Andrés López-Perrote, et al.,
bioRxiv - Biophysics 2020
Quote:
... coli cells (NZYTech) grown in LB medium ...
-
No products found
because this supplier's products are not listed.
Kashish Singh, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... coli cells (Genlantis) were used and the expression was induced overnight at 18°C with 0.1-0.25 mM IPTG added at O.D of 0.6-0.8 ...
-
No products found
because this supplier's products are not listed.
Ralf Schmidt, et al.,
bioRxiv - Immunology 2021
Quote:
... bulk T cells were frozen in Bambanker Cell Freezing Medium at 50e6 cells/ml (Bulldog Bio cat BB01) and stored at −80°C for short term or liquid nitrogen for long term storage ...
-
No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells at 2 × 106 cells/ml in BalanCD medium (Irvine Scientific) were transfected with 1 microgram plasmid/106 cells in 100-ml batches ...
-
No products found
because this supplier's products are not listed.
Chunyun Qi, et al.,
bioRxiv - Molecular Biology 2021
Quote:
cell viability was evaluated with the Cell Counting Kit-8 (AR1160, BOSTER) according to the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Joanne Kite, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cell-to-cell spread assays were carried out using pooled human serum (BioIVT) at a concentration of 10% to neutralise extracellular virus ...
-
No products found
because this supplier's products are not listed.
M. Chighizola, et al.,
bioRxiv - Biophysics 2020
Quote:
... and plated in low concentration of 4.000 cells/cm2 (to guarantee the presence of single separated cells) on Ø 40 mm glass-bottom dishes for cell culture (Willco Wells) the day before the experiment ...
-
No products found
because this supplier's products are not listed.
Kritika Khanna, et al.,
bioRxiv - Microbiology 2021
Quote:
... MEXi 293E cells (IBA Lifesciences) were cultured in MEXi culture medium (IBA Lifesciences ...
-
No products found
because this supplier's products are not listed.
Ty S. Maughon, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and one induced pluripotent stem cell derived MSC cell-line (Cellular Dynamics International, Madison WI) (Lot #0003 ...
-
No products found
because this supplier's products are not listed.
Sara A. Thannickal, et al.,
bioRxiv - Microbiology 2023
Quote:
BHK-21 cells (20,000 cells/well) were pretreated with increasing concentrations of either Imipramine (TCI America), Atorvastatin (TCI America) ...
-
No products found
because this supplier's products are not listed.
Niyati Jhaveri, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The flow cell (Akoya Biosciences, #240204) was assembled onto the slide as described in the user guide ...
-
No products found
because this supplier's products are not listed.
Sakura Kamiya, et al.,
bioRxiv - Pathology 2022
Quote:
... cells were seeded in 96-well plates (5000 cells/well) and treated with cisplatin (Adipogen AG, Liestal, Switzerland) and gefitinib (Cayman Chemical ...
-
No products found
because this supplier's products are not listed.
Rosario Distefano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... anti-Cox2 (ABclonal Technologies and Cell Signaling), anti-DSC2 (ABclonal Technologies) ...
-
No products found
because this supplier's products are not listed.
Nazila V. Jafari, Jennifer L. Rohn,
bioRxiv - Microbiology 2022
Quote:
HBLAK human bladder progenitor cells (CELLnTEC, Switzerland) were grown according to the CELLnTEC protocol ...
-
No products found
because this supplier's products are not listed.
Florian Wernert, et al.,
bioRxiv - Cell Biology 2023
Quote:
All live-cell experiments were performed in live-cell imaging medium (Hibernate E low fluorescence, Brainbits/Thermo Fisher Scientific #NC0285514) supplemented with 3% glucose ...
-
No products found
because this supplier's products are not listed.
Alberto Perez-Alvarez, et al.,
bioRxiv - Neuroscience 2019
Quote:
... inside a cell culture incubator (New Brunswick; Eppendorf) set to ∼37° C and ∼5% CO2 ...
-
No products found
because this supplier's products are not listed.
Jeffrey S. Bourgeois, et al.,
bioRxiv - Microbiology 2020
Quote:
... cells were stained with 7-aminoactinomycin D (Biomol) and analyzed on a Guava Easycyte Plus flow Cytometer (Millipore) ...
-
No products found
because this supplier's products are not listed.
Sandra Schwarz, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 20,000 IFNγ-treated tumor cells were seeded with 200,000 T cells in a 24-well plate coated with anti-human CD28 (Immunotools, Friesoythe, Germany) in DMEM/Ham’s F12 medium ...
-
No products found
because this supplier's products are not listed.
Cristiana Lungu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 24 h later the cells were imaged on a Zeiss Cell Observer SD Spinning Disk microscope equipped with an EMCCD camera (Photometrics Evolve 512). GFP was visualized with a 488 nm diode laser in combination with a FE01-520/35 nm emission filter ...