-
No products found
because this supplier's products are not listed.
Adil Mohamed, et al.,
bioRxiv - Microbiology 2019
Quote:
... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
-
No products found
because this supplier's products are not listed.
Oda C. Krokengen, et al.,
bioRxiv - Biochemistry 2024
Quote:
Sf9 insect cells cultured in Insect-XPRESS media (Lonza) were transfected with 2 µg of bacmid using Fugene 6 transfection reagent (Promega) ...
-
No products found
because this supplier's products are not listed.
Sarah W. Cai, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the P3 virus was used to infect Tni suspension insect cell culture (Expression Systems; Cat. 94-002S) for protein expression.
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No products found
because this supplier's products are not listed.
Thomas Labadie, Polly Roy,
bioRxiv - Microbiology 2020
Quote:
... KC cells (A Culicoides insect derived cell line) were maintained in Schneider′s Insect Medium (Sigma Aldrich), supplemented with 10% FCS and antibiotics (100 units/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Niloufar Ansari, et al.,
bioRxiv - Cell Biology 2020
Quote:
... A secondary antibody (swine anti-rabbit, Dako) was applied for 30 min at 1:300 ...
-
No products found
because this supplier's products are not listed.
Mariah Hassert, et al.,
bioRxiv - Microbiology 2020
Quote:
... Each virus was passaged in African green monkey kidney epithelial cells (Vero-WHO) that were purchased from American Type Culture Collection (ATCC CCL-81). The supernatants of these cultures were clarified of cellular debris by centrifugation at 3,500 RPM prior to being aliquoted and frozen at −80°C ...
-
No products found
because this supplier's products are not listed.
Desingu Ayyappa Raja, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... and 20% PANSERA (Pan Biotech; P30-2602) at 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Christopher Deich, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... insect Spodoptera frugiperda Sf21 cell line extract (Promega), and rabbit reticulocyte extract (Promega) ...
-
No products found
because this supplier's products are not listed.
Elizabeth Heyes, et al.,
bioRxiv - Cancer Biology 2023
Quote:
RNA from Cebpap30/p30 cell lines was isolated using RNeasy Plus Mini Kit (#74134 Qiagen) according to the manufacturer’s instructions and converted to cDNA with RevertAid First Strand cDNA Synthesis Kit (K1622) ...
-
No products found
because this supplier's products are not listed.
Dehao Huang, et al.,
bioRxiv - Immunology 2021
Quote:
... NKp30 (Biolegend, P30-15), NKp44 (Biolegend ...
-
No products found
because this supplier's products are not listed.
Joanna Domagala, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... clone p30-15 (BD Biosciences); anti-NKp44 ...
-
No products found
because this supplier's products are not listed.
Lydia Hadjeras, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... RNA was purified using Bio-Spin P30 columns (Bio-Rad). The RNAs were precipitated with 1 volume of absolute ethanol ...
-
No products found
because this supplier's products are not listed.
Romel Rosales Ramirez, Juan E. Ludert,
bioRxiv - Molecular Biology 2019
Quote:
The NS1 sequence from ZIKV (Mexican isolate, Asiatic linkage) and YFV NS1 (Brazilian yellow fever virus isolate) were synthesized de novo (GenScript, Piscataway, NJ). The synthetic ZIKV NS1 gene (GenBank accession number KY631493.1 ...
-
No products found
because this supplier's products are not listed.
Manon Laporte, et al.,
bioRxiv - Microbiology 2020
Quote:
... and mouse anti-MLV p30 antibody (Abcam, ab130757) as primary antibodies.
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No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
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No products found
because this supplier's products are not listed.
S.A. Codeluppi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and pZac2.1 gfaABC1D-lck-GCaMP6f virus (Addgene virus #52924).
-
No products found
because this supplier's products are not listed.
Sabina A. Kondratyeva, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... We obtained insect Sf9 cells from Merck and cultivated them in the Sf900 medium (Gibco ...
-
No products found
because this supplier's products are not listed.
Evelyn Ramberger, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... p30 or p30 R3L-BirA Ligase fragments were cloned into the pENTRY2B vector (Clontech #3064) using BamHI and XhoI restriction sites and subsequently introduced into the pInducer21 GFP lentiviral vector (Clontech #3044 ...
-
No products found
because this supplier's products are not listed.
Haig Yousef Babikian, et al.,
bioRxiv - Pathology 2020
Quote:
... We also prepared red blood cells from EDTA-treated swine blood by using Percoll (GE Healthcare, USA) and kept it in an RPMI 1640 medium (Gibco ...
-
No products found
because this supplier's products are not listed.
Alexandra L. Chang-Graham, et al.,
bioRxiv - Microbiology 2019
Quote:
African green monkey kidney (MA104) cells were cultured in DMEM supplemented with 10% (v/v) fetal bovine serum (Corning). Ito RV (G3[P8] ...
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No products found
because this supplier's products are not listed.
Uddhav Timilsina, et al.,
bioRxiv - Microbiology 2020
Quote:
... Concentrations of MLV p30 in the supernatants were determined using QuickTiter™ MuLV Core Antigen ELISA Kit (MuLV p30; Cell Biolabs). DNA was isolated from the infected cells using the DNeasy Blood and Tissue Kit (Qiagen ...
-
No products found
because this supplier's products are not listed.
Ann Logan, et al.,
bioRxiv - Immunology 2022
Quote:
... and yellow fever E antibody 3576 (Santa Cruz, sc-58083, for YF17D). The primary antibodies were detected with an Alexa-488 conjugate secondary antibody (Life Technologies ...
-
No products found
because this supplier's products are not listed.
Ferdos Abid Ali, et al.,
bioRxiv - Cell Biology 2023
Quote:
Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
-
No products found
because this supplier's products are not listed.
Ryoji Amamoto, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... retinas of adult CD1 mice (>P30) from Charles River Laboratories were dissected ...
-
No products found
because this supplier's products are not listed.
María Laura Kevorkian, et al.,
bioRxiv - Microbiology 2022
Quote:
... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
-
No products found
because this supplier's products are not listed.
Mohini Bhupathi, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Typhi (the bacterial pathogen that causes typhoid fever) using 2X Warmstart colorimetric LAMP (M1800, NEB, US). The RNA template of the SARS-CoV-2 N gene was synthesised using in-vitro transcription (Hiscribe ...
-
No products found
because this supplier's products are not listed.
Sijie Yang, et al.,
bioRxiv - Immunology 2024
Quote:
... the 293T cells were infected with G*ΔG-VSV virus (VSV-G pseudotyped virus, Kerafast) in the cell culture supernatant ...
-
No products found
because this supplier's products are not listed.
Yidong Wang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... ZR Tissue & Insect RNA MicroPrep™ (Zymo Research Corp. ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Jill E Hunter, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... followed by 20% swine serum in PBS and then incubated with primary antibodies overnight at 4 °C (γH2AX ((#9718, Cell Signaling Technologies). The following day ...
-
No products found
because this supplier's products are not listed.
Xin Sheng, et al.,
bioRxiv - Genomics 2022
Quote:
... These four datasets were ELLIPSE OncoArray (African ancestry, 4231 cases/3953 controls on Illumina Consortium-OncoArray ...
-
No products found
because this supplier's products are not listed.
Sabal Chaulagain, et al.,
bioRxiv - Microbiology 2020
Quote:
Swine PBMCs were obtained from swine whole blood through density gradient centrifugation using Sepmate15 (Stem cell technologies) and lymphocyte separation media (Corning) ...
-
No products found
because this supplier's products are not listed.
Shuo Du, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Sf21 insect cells grown in SIM SF medium (Sino Biological Inc.) were used to generate and amplify the baculoviruses ...
-
No products found
because this supplier's products are not listed.
Yoshitomo Kurogi, et al.,
bioRxiv - Physiology 2022
Quote:
... The dissected tissues were held in a glass-bottom dish (35 × 10 mm, IWAKI #3910-035) with an insect pin (φ0.10 mm, Ento Sphinx Insect Pins) and silicone grease (Beckman), and 20 μL of SDM was added to cover the tissue ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
Huy-Dung Hoang, et al.,
bioRxiv - Molecular Biology 2019
Quote:
Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ...
-
No products found
because this supplier's products are not listed.
Jonathan Leis, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
-
No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated with anti-Dengue virus NS3 protein antibody (GeneTex: GTX124252) at 1:1000 for two hours ...
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... the virus-resistant cell population was transferred to fresh 150-mm dishes (Greiner Bio-One) and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Susie S. Cha, Mark E. Bucklin, Xue Han,
bioRxiv - Bioengineering 2020
Quote:
... The micropipette was then frontloaded with virus or cells using a controller (WPI, Sys-Micro4). In general ...
-
No products found
because this supplier's products are not listed.
Jessie J.-Y. Chang, et al.,
bioRxiv - Immunology 2023
Quote:
... propagation of the virus was carried out in Vero (African green monkey kidney epithelial – ATCC: CCL-81) cells cultured in MEM (MP Biomedicals), supplemented with 1 µg/mL TPCK-Trypsin (Trypsin-Worthington) ...
-
No products found
because this supplier's products are not listed.
Lin Xie, et al.,
bioRxiv - Cell Biology 2023
Quote:
... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
-
No products found
because this supplier's products are not listed.
Stamatina Tzanoulinou, et al.,
bioRxiv - Neuroscience 2021
Quote:
... C57Bl/6j wildtype pups or >P30 mice were placed on a stereotaxic frame (Angle One; Leica, Germany). For the pups ...
-
No products found
because this supplier's products are not listed.
Sayumi Oishi, et al.,
bioRxiv - Microbiology 2023
Quote:
The insects were dissected under a stereomicroscope (SZ61, Olympus, Japan) in phosphate buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Alexander Gusev, et al.,
bioRxiv - Genetics 2019
Quote:
... Virus transduced cells were maintained for 2 weeks under blasticidin (10 μg/ml, Invivogen) selection ...
-
No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
-
No products found
because this supplier's products are not listed.
Victor Calbiague Garcia, et al.,
bioRxiv - Physiology 2023
Quote:
Retinal slices from p30 ex vivo retinas were visualized with an upright microscope (Nikon Eclipse FN1) equipped with a 40x water-immersion objective ...
-
No products found
because this supplier's products are not listed.
Dawei Zhou, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Percentages of virus-infected cells was quantified by using the Gen5 Image+ software (BioTek).
-
No products found
because this supplier's products are not listed.
Enakshi Roy, Wen Shi, Bin Duan, St Patrick Reid,
bioRxiv - Microbiology 2020
Quote:
... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
-
No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...